反相HPLC法测定血清中氯化水杨醛呋喃硫羰基腙合铜

应用Ｓｈｉｍ－ｐａｃｋＣＬＣ－ＯＤＳ柱设计了测定家兔血清中新型杀菌剂氯化水杨醛呋喃硫羰基腙合铜浓度的反相ＨＰＬＣ方法。活性化合物的平均绝对和相对回收率分别为８４．３６％和９９．８０％；日内（ｎ＝５）和日间（ｎ＝１５）的平均变异系数分别为４．８９％和５．７０％；最低检出浓度为０．０２ｍｇ／Ｌ，线性范围为０．０５～２０ｍｇ／Ｌ（ｎ＝９，ｒ＝０．９９９９）。     

大黄素对大鼠腹腔巨噬细胞生物合成白三烯B4的影响

研究不同浓度大黄素对大鼠腹腔巨噬细胞（ＭΦ）生物合成白三烯Ｂ_４（ＬＴＢ_４）的影响。结果表明大黄素在５×１０￣（－７）～５×１０￣（－６）ｍｏｌ／Ｌ浓度范围内（ｎ＝４），可抑制大鼠腹腔ＭΦＬＴＢ_４的生物合成，量效关系良好（ｒ＝０．９５９１，ｎ＝４）；大黄素对大鼠腹腔ＭΦ生物合成ＬＴＢ_４的抑制率范围为２８．４±６．７％～８８．０±５．３％。大黄素５×１０￣（－６）ｍｏｌ／Ｌ，相当于氟灭酸１×１０￣（－４）ｍｏｌ／Ｌ抑制作用；ＬＴＢ_４的半数抑制率（ＩＣ_（５０））为１．２×１０￣（－６）ｍｏｌ／Ｌ。总之ＥＭＯ的抗炎机制与抑制ＭΦ生物合成ＬＴＢ_４有关。     

H-point计算分光光度法研究及其在复方盐酸丁卡因注射液中的应用

介绍几种Ｈ－ｐｏｉｎｔ法,并与常见的计算分光光度法进行了比较。将Ｈ－ｐｏｉｎｔ法用于复方盐酸丁卡因注射液测定的数据处理,表明Ｈ－ｐｏｉｎｔ法有效地消除干扰,有较高的精密度和准确度。盐酸丁卡因回收率为：Ｈ－ｐｏｉｎｔ法１０１．０％,ＲＳＤ为２．７％（ｎ＝７）,Ｋ系数Ｈ－ｐｏｉｎｔ法９９．１％,ＲＳＤ为１．８％（ｎ＝７）,三波长Ｈ－ｐｏｉｎｔ法９９．６％,ＲＳＤ为１．４％（ｎ＝７）。     

高效液相色谱法同时测定制马钱子中士的宁和马钱子碱的含量

建立了用高效液相色谱法同时测定制马钱子中士的宁和马钱子碱的含量。氯仿－浓氨溶液（９０∶１０）作溶剂，超声波振荡提取。硅胶柱，氯仿－甲醇－浓氨溶液（９０∶１０∶０．３）为流动相，紫外２５４ｎｍ波长检测。平均加样回收率（ｎ＝５）士的宁为９８．２％，ＲＳＤ＝１．４％；马钱子碱为９９．３％，ＲＳＤ＝３．３％。日内、日间精密度（ＲＳＤ）士的宁分别为０．６％和１．３％；马钱子碱分别为１．１％和２．４％。     

用高专属性酶分析法测定果糖─1，6－二磷酸及其水针剂的稳定性

利用酶分析法测定果糖－１，６－二磷酸（ＦＤＰ）及其水针剂的稳定性。结果：Ｙ＝０．００８＋１．１０３ｃ，ｒ＝０．９９９８（ｎ＝３）：准确度为９９．９９±２．５７％（ｎ＝５）：天内、天间的ＲＳＤ分别为０．７０％和１．１０％（ｎ＝４）：且不受葡萄糖、果糖的干扰；经４０℃加速试验，强光照射试验及高温试验，ＦＤＰ水针剂的稳定性均好。结论：本法的准确度高、专一性好；我们试制的ＰＤＰ水针剂稳定性好，具有应用价值，值得开发。     

高效液相色谱法测定康咳复方片中盐酸伪麻黄碱和美沙芬的含量

报道了新药康咳复方片中盐酸伪麻黄碱和美沙芬的高效液相色谱测定法。固定相为Ｎｕｃｌｅｏｓｉｌ－ＯＤＳ柱，流动相为１０ｍｍｏｌ／Ｌ＋二烷基硫酸钠（ＳＤＳ）水溶济－乙腈（６５：３５，ｐＨ３．５），检测波长为２６０ｎｍ。盐酸伪麻黄碱和美沙芬回归方程及线性范围分别为：１．２～６．２μｇ，Ｙ＝１．０１６×１０５Ｘ＋１．０８７×１０２（ｎ＝５），ｒ＝０．９９９８；０．６～３．０μｇ，Ｙ＝２．００７×１０４Ｘ－１．０２４×１０２（ｎ＝５），ｒ＝０．９９９９。平均回收率分别为９８．９０％±０．９１％，ＲＳＤ＝０．９５％（ｎ＝７）；９９．０４±１．１２％，ＲＳＤ＝１．００％（ｎ＝７）。     

高压液相色谱法测定安神二号蜜丸和赤芍中芍药甙含量的研究

用高压液相色谱法测定安神二号蜜丸制剂和赤芍中芍药甙的含量，以寻找控制制剂和药材质量的方法。安神二号蜜丸和赤芍饮片经冷浸、温浸、回流、超声处理，芍药甙提取完全。采用ＹＷＧＣ１８不锈钢柱（１５０／４．６／１０），以甲醇／０．０５ｍｏｌ（ｐＨ３．６）磷酸二氢钾（３５：６５）为流动相，在流速０．８ｍｌ／ｍｉｎ、工作压力２２６００ｋＰａ、检测波长２４０ｎｍ的条件下，芍药甙ｔＲ为６．１０ｍｉｎ，单一样本测定时间为１２ｍｉｎ。芍药甙在２～４０μｇ／ｍｌ范围内线性关系良好，ｒ＝０．９９９８（ｎ＝５），回收率为９８．０３％，ＣＶ＝０．４４％（ｎ＝３）。日内误差为２．２５％（ｎ＝６），日间误差为２．２４％（ｎ＝６）。安神二号蜜丸和赤芍中芍药甙的含量均值分别为０．３１８％（ｎ＝３）和４．５２％（ｎ＝３），ＣＶ分别为２．６３％和２．３１％。     

力可生颗粒剂中芦丁及总甙含量测定

用ＲＰ—ＨＰＬＣ法测定力可生颗粒剂中芦丁含量，相关系数ｒ＝０．９９９９，线性范围：０．００２６４～０．０４２２４（ｍｇ／ｍｌ），平均回收率为９９．０％，ＲＳＤ＝１．９０％（ｎ＝９）；采用分光光度法测定本品中总甙含量，相关系数ｒ＝０．９９９４，人参皂甙Ｒｅ的线性范围：０．０４～０．２４（ｍｇ／６ｍｌ），平均回收率分别为１０２．６％、ＲＳＤ＝１．４６％（ｎ＝４），１０３．５％、ＲＳＤ＝０．８７％（ｎ＝４）。由此可见，上述测定方法准确、可行。     

86例小儿气管异物取出术麻醉体会

行小儿气管异物取出术８６例，根据不同麻醉用药分为４组：Ⅰ（ｎ＝２９）氯胺酮５ｍｇ·ｋｇ－１肌注，γ－ＯＨ８１．５～１０８．６ｍｇ·ｋｇ－１静注；Ⅱ（ｎ＝２４）氯胺酮５ｍｇ·ｋｇ－１肌注，安定０．２ｍｇ·ｋｇ－１、γ－ＯＨ３７．５～６５．５ｍｇ·ｋｇ－１静注；Ⅲ（ｎ＝２３）氯胺酮５ｍｇ·ｋｇ－１肌注，安定０．２ｍｇ·ｋｇ－１静注；Ⅳ（ｎ＝１０）单用氯胺酮５ｍｇ·ｋｇ－１肌注。各组优例数率与并发症发生率分别为：Ⅰ８２．８％和６．９％，Ⅱ６６．７％和１２．５％，Ⅲ４３．５％和２１．７％，Ⅳ０和４０％。说明氯胺酮合用γ－ＯＨ较大剂量的Ⅰ组麻醉效果最好，并发症发生率最低；单用氯胺酮的Ⅳ组麻醉效果最差，并发症发生率最高。     

血浆脂蛋白脂酶及肝脂酶的比色测定法

为探讨高脂血症、动脉粥样硬化的发病机理和脂蛋白代谢，作者建立了同时测定肝素化后血浆脂蛋白脂酶（ＬＰＬ）和肝脂酶（ＨＬ）活性的比色测定法。该法以脂肪乳剂为底物，在３７℃、ｐＨ８．３的条件下与一定量肝素后血浆保温３０分钟，血浆中的ＬＰＬ及ＨＬ可将底物中的甘油三酯（ＴＧ）水解为甘油和游离脂肪酸（ＦＦＡ）。用铜试剂法测定生成的ＦＦＡ的量，即可分别计算出ＬＰＬ和ＨＬ的活性。同时，对ＬＰＬ和ＨＬ的动力学进行了观察，ＬＰＬ对ＴＧ的Ｋｍ值为０．９μｍｏｌ／Ｌ，ＨＬ为２．４μｍｏｌ／Ｌ。ＬＰＬ及ＨＬ测定的变异系数分别为４．５％（ｎ＝４），２．９％（ｎ＝６）和６．４％（ｎ＝６），４．８％（ｎ＝６）。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.