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1.
Objective To identify the changes in serum insulin like growth factor-Ⅰ (IGF-Ⅰ) and IGF binding proteins (IGFBPs) in children with nephrotic syndrome (NS) and the effect of glucocorticoid on serum IGF-Ⅰ and IGFBPs. Methods We measured serum IGF-Ⅰ and IGFBPs levels by radioimmune assay and immune radiomagnetic assay in 36 children with NS, consisting of an active stage group (ANS, n=12), a remission stage group (RE, n=12), an active stage group with glucocorticoid treatment (GNS, n=12), and a normal control group (NC, n=10). Results 1) Compared to NC, serum levels of IGF-Ⅰ and IGFBP-3 were decreased (P<0.01); serum levels of IGFBP-1 and IGFBP-2 were increased (P<0.01) in the ANS group. 2) Serum levels of IGF-Ⅰ and IGFBP-3 were higher and IGFBP-1 and IGFBP-2 were lower in the RE Group than in theANS Group (P<0.01). 3) Compared to the ANS group, serum levels of IGF-Ⅰ and IGFBP-3 were increased (P<0.01) and serum levels of IGFBP-1 and IGFBP-2 were decreased (P<0.01) in the GNS group. 4) A correlation was found between serum levels of IGFBP-3 and albumin in the active stage group (r=0.76 P<0.01). There was also a correlation between serum levels of IGF-Ⅰ and IGFBP-3 and an inverse correlation between the serum level of IGF-Ⅰ and serum levels of IGFBP-1 and IGFBP-2 in the ANS group. No other correlations were observed.Conclusions The serum levels of IGF-Ⅰ and IGFBPs are altered in children in the active stage of NS, but return to normal in the remission stage. GC treatment may influence serum IGF-Ⅰ and IGFBPs in children with NS. Changes in IGF-Ⅰ and IGFBPs levels may play a role in the growth retardation of NS children.  相似文献   

2.
An epidemiological survey on neonatal jaundice in China   总被引:2,自引:0,他引:2  
Objective To provide epidemiological data for revising the diagnostic criteria of neonatal hyperbilirubinemia in China. Methods A survey was performed among full-term infants in multiple centers throughout the country. From less than 24 hours after birth, the infants’ bilirubin levels were measured every day until the peak level fell to less than 68.4 μmol/L. Auditory brainstem responses were assessed in 56 infants randomly chosen from those with serum bilirubin levels of higher than 220.5 μmol/L. Results Jaundice in most infants was detected at 2-3 days after birth. The bilirubin level usually reached a peak level of 204±54.69 μmol/L at 5 days after birth and then fell. Among the 875 infants, the serum bilirubin levels in 34.4% of neonates were higher than 220.5 μmol/L. The mean serum bilirubin level of the infants during the first week after birth varied with geography (P<0.001) and season (P<0.001). The serum bilirubin level was significantly associated with gestation age (P<0.01), delivery method (P<0.01), weight loss (P<0.001), and PCV elevation (P<0.001) during the first three days after birth. Conclusions The start time of neonatal jaundice was similar to that reported elsewhere, but the mean peak level in our study was higher than the reported. It is suggested that the diagnostic criteria for neonatal hyperbilirubinemia in China should be strict.  相似文献   

3.
Objective To elucidate the airway responsiveness and its relative factors in asthma with natural remission at puberty. Method The airway responsiveness was evaluated by provocation of methacholine (PC20). Blood sIL-2R, IL-4 and IFN-γ values in the cultural supernatant of mononuclear cells, eosinophil count, basophil count and basophil releasability were detected in 18 asthmatic cases with remission at puberty (group Ⅰ), in 20 asthmatics without remission at puberty (group Ⅱ), and in 30 healthy controls (group Ⅲ). Result The mean value of PC20 in group Ⅰ was significantly lower than that in group Ⅲ (P<0.01), but still higher than that in group Ⅱ (P<0.01). The blood sIL-2R, and eosinophil counts dropped almost to those of group Ⅲ, significantly lower than those of group Ⅱ. IL-4 in the culture supernatant of mononuclear cells was similar to group Ⅲ, but significantly lower than that in group Ⅱ. However IFN-γ was also similar to group Ⅲ, significantly higher than that of group Ⅱ. The basophil count and positive rate of human basophil degranulation test stimulated by mannitol with hyper-osmolarity were still abnormal, significantly higher than those of group Ⅲ, and the basophil level was significantly related to the airway responsiveness. Conclusion The asthmatics with remission at puberty have a certain degree of airway hyper-responsiveness that is related to the abnormality of basophil.  相似文献   

4.
Objective To explore the relationship of β-adrenoreceptor density and function with the condition of patients with essential hypertension.Methods In the present study, 69 male patients with essential hypertension at different stages were compared with a group of age-matched normotensive controls. β-ad renoreceptor maximum bound volume (B(max)) in peripheral lymphocytes was mea sured by (3)H-dihydroalprenolol ((3)H-DHA) radio ligand binding. β- adrenoreceptor responsiveness was determined by Salbutamol (injection). Results In patients with essential hypertension at stages Ⅰ and Ⅱ, B(max) was si gnificantly higher (P<0.01 and P<0.001, respectively) and the chronot ropic doses of Salbutamol required to increase the heart rate by 30 beats/min (CD(30)) were significantly lower (P<0.01 and P<0.001, respect ively) than in age-matched normotensive control subjects. In patients with ess ential hypertension at stage Ⅲ, B(max) was significantly lower and CD(30)was significantly higher (both P<0.01) than those in the age-matched n ormotensive control subjects. B(max) was significantly higher and CD(30) was significantly lower (both P<0.001) in patients with essential hyper tensi on and with left ventricular hypertrophy (LVH) than that in patients with essent ial hypertension but without LVH. In patients with essential hypertension and heart failure, B(max) was significantly lower and CD(30) was significant ly higher (both P<0.001) than those in patients with essential hypertension without heart failure.Conclusions The changes of β-adrenoreceptor density and function were related to hypertens ion, hypertension complicated with ventricular hypertrophy, and heart failure. They may be viewed as indexes of the condition in the patients with essential hy pertension.  相似文献   

5.
Objective To discuss the roles of serum interleukin-18 (IL-18), interleukin-10 (IL-10) and soluble interleukin-2R (sIL-2R) in the pathogenesis of chronic hepatitis C and to observe the effects of interferon (IFN) on the above- mentioned serum cytokines. Methods The levels of above- mentioned cytokines were detected in 10 healthy individuals, 24 asymptomatic hepatitis virus C (HCV) carriers and 27 patients with chronic hepatitis C ( before and after IFN treatment) using enzyme linked immunosorbent assay (ELISA). Results The levels of the cytokines in patients with chronic hepatitis C are higher than in healthy people (P<0.05) and in asymptomatic HCV carriers(P<0.05). The values of the cytokines show a significant positive correlation to ALT (P<0.05). Levels of tested cytokines decreased observably after IFN treatment (P<0.05). The grades of the serum levels for sIL-2R and IL-10 before IFN treatment (from high to low) were categorized accordingly: non-response group> partial- response group >complete- response group (P<0.05). Conclusions The tested cytokines co-participate in the pathogenesis of chronic hepatitis C, and can be used to evaluate the effect of IFN on the immune state of organisms. Furthermore, sIL-2R and IL-10 are important for predicting the anti-viral efficacy of IFN.  相似文献   

6.
Changes in intestinal microflora in patients with chronic severe hepatitis   总被引:14,自引:0,他引:14  
Objective To investigate changes in intestinal microflora in patients with chronic severe hepatitis (CSH), and their role in this life-threatening disease.Methods We classified nineteen patients with chronic severe hepatitis as the CSH group, thirty patients with chronic hepatitis (CH) as the CH group and thirty-one heal thy volunteer as the control group. Fecal flora from all subjects were analyzed . Concentrations of plasma endotoxin, serum cytokines tumor necrosis factor alp ha (TNF-α) and interleukin-1 beta (IL-1β) and liver function were assessed .Results The number of fecal bifidobacterium (P<0.001, P<0.05 respectively), as well as bacteroidaceae (P<0.001, P<0.01 respectively) were significan tly deceased in patients with chronic severe hepatitis compared with the CH and control groups, while the number of enterobacteriaceae (P<0.001, P< 0.05 respectively) and yeasts (P<0.01, P<0.05 respectively) were sign ificantly increased. Levels of plasma endotoxin, serum TNF-α, IL-1β and tot al bilirubin (TBiL) were significantly increased in the CSH group. The concentr ation of endotoxin positively correlated with levels of both TNF-α, IL-1β an d TBiL (P<0.001, respectively). Levels of plasma endotoxin were positivel y correlated with the number of fecal enterobacteriaceae and negatively correlat ed with bifidobacterium (P<0.05, P<0.001, respectively).Conclusion Intestinal flora in patients with chronic severe hepatitis were severely disturb ed and gut mircobiological colonization resistance was impaired. Changes in int estinal flora may have a pivotal role in both the elevation of plasma endotoxin and further hepatic lesions resulting in liver failure.  相似文献   

7.
8.
Objectives To study the influence of insulin on IGF-Ⅰ and IGFBP-Ⅰ secretion of the human endometrial stromal cells. Methods Late proliferative phase endometrial stromal cells were isolated from endometrium tissues and then cultured for 24 h in Hams F-12 only as a control and in Hams F-12 with different concentrations of estradiol (E2) and insulin (INS) as treated groups. Simultaneously, the endometrial stromal cells from late secretory phase endometrium were cultured for 24 h in Hams F-12 only as a control and in Hams F-12 supplemented with different concentrations of progesterone (P) and insulin as treated groups. After 24 h of culturing, the mediums were collected for either IGF-Ⅰ or IGFBP-Ⅰ assays. Result The concentrations of IGF-Ⅰ in medium from cultured endometrial stromal cells in the proliferative phase were 0.78±0.47 ng/ml in the hormone-free control group; 1.44±0.59 ng/ml and 1.39± 0.33 ng/ml in 100 pg/ml E2 group and 20 μU/ml INS group, which was higher than that of the control group (P<0.05 and P<0.01, respectively). The IGF-Ⅰ concentration in the 100 μU/ml INS group was 2.03±0.53 ng/ml, which was higher than that of the 20 μU/ml INS group (P<0.01). Levels of IGF-Ⅰ in the 100 pg/ml E2 plus 20 μU/ml INS group was 2.18±0.36 ng/ml, which was significantly higher than that of the 20 μU/ml INS and 100 pg/ml E2 group (P<0.01), but lower than that of the 100 pg/ml E2 plus 100 μU/ml INS group (3.42±0.75 ng/ml), P<0.01. The concentration of IGFBP-Ⅰ in medium from cultured endometrial stromal cells in the secretory phase was 2.50±1.39 ng/ml in the hormone-free control group and 5.44±2.09 ng/ml in the 10 pg/ml P group, which was significantly higher than that of the control (P<0.01). IGFBP-Ⅰ concentration in 20 μU/ml INS group was 0.16±0.58 ng/ml, which was lower compared with control, but higher compared with the 100 μU/ml INS group (P<0.01). The level of IGFBP-Ⅰ in the 10 ng/ml P plus 20 μU/ml INS group was 2.10±1.17 ng/ml, lower compared with the 10 ng/ml P group, but higher compared with the 10 pg/ml P plus 100 μU/ml INS group, P<0.01. Conclusions Insulin can stimulate basal (without hormone) and E2-stimulated IGF-Ⅰ secretion in cultured stromal cells from human late proliferative endometrium in a dose-dependent manner. Insulin can suppress basal (without hormone) and P-stimulated IGFBP-Ⅰ secretions in cultured stromal cells from human secretory endometrium in a dose-dependent manner.  相似文献   

9.
Objective To understand the interaction between surfactant proteins and pneumocys tis carinii pneumonia (PCP), and the impact of corticosteriods on surfactant proteins.Methods We established rat models of PCP and bacterial pneumonia induced by subcutaneous injection of 25mg cortisone acetate. At 8-12 wk, the bronchoalveolar lavage f luid (BALF) of rats was collected. Total nucleated cells of BALF were counted a nd differentiated, and the concentrations of surfactant protein A (SP- A) and su rfactant protein D (SP- D) were measured by immunoblotting assay. The rats were divided into three immunosuppressive groups and a normal control group. Group Ⅰ, normal control (n=6), consisted of healthy SD rats; group Ⅱ, negative cont rol (n=6), consisted of rats with cortisone acetate injection for over 8 wk with out lung infection; group Ⅲ, bacterial pneumonia (n=11), rats were injected wit h cortisone acetate over 8 wk that resulted in bacterial pneumonia without other pathogens isolated; and group Ⅳ, PCP (n=14), rats with injected cortisone acet ate for 8-12 wk and developed PCP without other pathogens isolated. Results Our results indicated that the total cell count in BALF in the negative control group was lower than that in the normal control group (P<0.001). During P CP infection, the total cell count and the percentage of polymorphonuclearcytes (PMNs) in BALF were signi ficantly increased (P<0.01), but were lower than those in the bacterial pne umonia group. The concentration of SP- A of BALF in PCP (45.1±22.1 μg/ml) was significantly increased in comparison with that in the negative control ( 16.2±9.9 μg/ml, P<0.05) and bacterial pneumonia groups (6.2±5.6 μ g/ml, P<0.001). We also found that the relative content of SP - D was signi ficantly higher in PCP (24 249±4780 grey values) than that in the negative control (13 384±2887 grey values, P<0.001) and that in bacterial pne u monia (11 989±2750 grey values, P<0.001). SP- A and SP- D were a lso higher in the moderate to heavy group of PCP than those seen in the mild group (P<0.01, P<0.001). SP- A and SP- D were higher in the negative contr ol group than those in the normal control group, but there was no significant di fference between the 2 groups. Conclusion These results suggest that the concentrations of SP- A and SP- D in BALF are inc reased by pneumocystis carinii specific stimulation, but the alteration is n ot related to the corticosteriod usage.  相似文献   

10.
TNF-α and IL-8 of the Patients with Allergic Asthma   总被引:1,自引:0,他引:1  
The levels of serum TNF-α and IL-8 in the patients with allergic asthma during acute attack period and remission period, and the effects of glucocorticoid (GC) on them were investigated. By using ELISA, the levels of TNF-α and IL-8 were detected in the healthy volunteers (group C, n=40), the patients with allergic asthma (n=40) during acute attack period (group A) and remission period (group B) and those taking GC for a week (n=28). The results were compared among them. It was found that the levels of TNF-α and IL-8 in group A were higher than in group B and group C. In the patients subject to GC therapy, the levels of TNF-α and IL-8 were decreased as compared with those in group A. In group B, the level of TNF-α was higher than in group C, but there was no significant difference in the level of IL-8 between group B and group C. It was concluded that the inflammatory cytokines, TNF-α and 11.-8, played important roles in the bronchus allergic inflammation. GC could reduce the levels of serum TNF-α and IL-8 to exert the anti-inflammatory effects.  相似文献   

11.
目的 探讨原发性干燥综合征(pSS)患者外周血清中肿瘤坏死因子α(TNF-α)、转化生长因子β1(TGF-β1)表达水平与患者唇腺淋巴细胞浸润程度及肺间质病变(ILD)的相关性.方法 采用酶联免疫吸附测定法(ELISA)测定116例pSS患者及20例健康体检者(对照组)血清TNF-α、TGF-β1水平,同时对pSS患者唇腺标本进行病理检测.根据肺间质纤维化病变分期及唇腺病理分级将116例pSS患者分为单纯pSS组(n=68)、pSS-ILD组(n=48)及无浸润组(n=36)、浸润组(n=80),对血清TNF-α、TGF-β1水平进行统计学分析.结果 pSS-ILD组患者血清TNF-α、TGF-β1水平明显高于单纯pSS组和对照组(P<0.01),单纯pSS组患者血清TNF-α、TGF-β1水平明显高于对照组(P<0.01);唇腺淋巴细胞浸润组患者血清TNF-α、TGF-β1水平明显高于无唇腺淋巴细胞浸润组和对照组(P<0.01),无唇腺淋巴细胞浸润组患者血清TNF-α、TGF-β1水平明显高于对照组(P<0.01).TNF-α、TGF-β1表达与淋巴细胞浸润灶分级及肺间质纤维化病变分期呈正相关(r=0.867、0.613、0.814、0.864,P=0.000);TNF-α与TGF-β1的相对表达量呈正相关(r=0.857,P=0.000).结论 TNF-α、TGF-β1参与了pSS患者唇腺淋巴细胞浸润及ILD的发生、发展,二者表达增加可能是pSS患者唇腺结构破坏及合并ILD的原因.  相似文献   

12.
目的 探讨分泌性中耳炎(secretory otitis media,SOM)患者血清及耳积液内炎性因子和转化因子水平变化,并分析指标水平与SOM的相关性。 方法 选取2015年10月-2017年1月浙江省立同德医院收治的SOM患者作为观察对象,分为急性组、亚急性组和慢性组,每组各抽取患者30例,同时选取30例健康体检者作为对照组;比较各组患者血清、耳积液内的炎性因子和转化因子水平,并分析各指标与SOM的相关性,其中多组间指标水平比较采用单因素方差分析,组间两两比较采用t检验,相关性分析采用Spearman分析。 结果 SOM患者与对照组血清及SOM患者中耳积液内的炎性因子与转化因子水平比较差异均有统计学意义(均P<0.05);与对照组相比,SOM患者血清TNF-α、IL-5、IL-8、TGF-β1和TGF-β2水平均显著升高,差异均有统计学意义(均P<0.05);与急性组相比,亚急性组及慢性组的血清及中耳积液内的TNF-α、IL-5、IL-8、TGF-β1和TGF-β2水平均显著升高,且慢性组各指标水平显著高于亚急性组,差异均有统计学意义(均P<0.05);相关性分析结果表明,SOM患者血清及中耳积液内的TNF-α、IL-5、IL-8、TGF-β1和TGF-β2水平与SOM病情严重程度呈正相关(均P<0.05)。 结论 SOM患者血清炎性因子及转化生长因子水平异常,血清及耳积液内炎性因子、转化生长因子水平与SOM的病情严重程度关系较为密切。   相似文献   

13.
糖肾宁对早期糖尿病肾病细胞因子的影响研究   总被引:1,自引:0,他引:1  
目的:探讨糖肾宁对早期糖尿病肾病(DN)细胞因子的影响。方法:对78例早期DN(Ⅲ期)患者采用中心分层、区组随机化方法分为治疗组(糖肾宁组)与对照组(西药常规组)。对照组38例患者采用替米沙坦80mg,1次/d进行治疗。治疗组在对照组基础上每天加服中药糖肾宁1剂,分2次口服,8周为一疗程。采用双抗体夹心酶联免疫法(ELISA法)检测78例早期DN患者治疗前、治疗4周、治疗8周后血清和尿液中白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)及转化生长因子-β1(TGF-β1)水平的变化。结果:两组治疗前血、尿IL-6、TNF-a、TGF-β1水平明显高于健康组(P<0.01);随着治疗时间的延长两组血、尿中上述细胞因子水平逐步下降,但治疗组在治疗4周和8周后均下降明显(P<0.01);对照组4周时无明显变化;8周后也下降(P<0.05)。对照组TGF-β1治疗4周、8周均无明显变化;治疗组与对照组同期血、尿中IL-6、TNF-α、TGF-β1水平比较,差异有统计学意义(P<0.01)。结论:细胞因子与DN的发病、发展有密切关系,糖肾宁对血、尿中IL-6、TNF-α、TGF-β1有明显的下调作用。  相似文献   

14.
目的探讨炎症细胞因子在慢性阻塞性肺疾病患者治疗前后的变化。方法选择45例急性发作期的COPD患者(观察组),入院后予以吸氧、抗感染、止咳化痰及平喘等常规对症治疗1周。观察治疗前后血浆肿瘤坏死因子-α(TNF-α)、转化生长因子(TGF-β1)和白介素~IO(IL-10)水平的变化。另选择我院体检中一t7正常的健康体检者30例作为对照组。结果观察组的血浆TNF-α和TGF-β1水平明显高于对照组,IL-10水平明显低于对照组(均P〈0.01);治疗1周后,观察组的血浆TNF-α和TGF-β1水平较治疗前明显下降,血浆IL-10水平较治疗前明显上升(均P〈0.01)。结论TNF-α、TGF-β1和IL-10参与了COPD发病过程,通过调节血浆炎症细胞因子的水平,为COPD治疗提供新的思路。  相似文献   

15.
目的:通过对溃疡性结肠炎(Ulcerative Colitis,UC)组织中白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)和转化生长因子-β1(TGF-β1)表达水平的检测,探讨它们在UC发生、发展过程中的作用。方法:①实验分组:UC组(90例);正常对照组(30例)。②IL-8、TNF-α和TGF-β1表达水平的检测:应用放射免疫法(RIA)检测三者在UC组、正常对照组血清中的表达水平。结果:UC组血清中IL-8、TNF-α表达水平与正常对照组相比明显增高(P<0.01),并与临床上病情的严重程度相一致;血清中TGF-β1的含量UC组高于正常对照组(P<0.05)。结论:UC组IL-8、TNF-α和TGF-β1的含量明显升高,提示IL-8、TNF-α和TGF-β1在UC的发病机制中起重要作用。  相似文献   

16.
目的:探讨细胞因子白介素-10(interleukin-10,IL-10)、肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、转化生长因子-β(transforming growth factor-beta,TGF-β)在慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)发病机制及加重过程中的作用。方法:采用ELISA测定COPD急性加重期、缓解期及健康对照组血清IL-10、TNF-α、TGF-β水平。结果:COPD急性加重期及缓解期患者血清IL-10水平均低于正常对照组(P〈0.01);COPD急性加重期及缓解期组TNF-α水平明显升高,均高于正常对照组(P〈0.01);COPD急性加重期血清TGF-β浓度均高于缓解期及正常对照组(P〈0.01),但缓解期与正常对照组差异无统计学意义(P〉0.05)。COPD急性加重期血清TGF-β水平与IL-10呈正相关关系。结论:IL-10、TNF-α、TGF-β参与COPD发病机制及急性加重过程。IL-10作为炎症抑制因子,其血清水平降低,可能在COPD气道炎症反应以及炎症加剧的过程中起着重要作用。  相似文献   

17.
目的::探讨JAK2/STAT3信号通路在白藜芦醇抗肝脏缺血再灌注损伤中的作用及机制。方法:健康雄性SD大鼠40只随机分为4组,假手术对照(SC)组、肝脏缺血再灌注(HIR)组、白藜芦醇处理(Res+HIR)组、JAK2/STAT3通路阻断剂AG490(Res+AG490+HIR)组。建立急性肝脏缺血再灌注损伤模型,于再灌注6h后收集动物血液和肝脏标本。检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(AKP)及炎症因子IL-6、TNF-α、IL-10、TGF-β的变化。肝脏组织HE染色,光镜下观察肝组织形态学变化。结果:与HIR组相比,Res+HIR组大鼠肝组织病理学变化明显改善,血清中反映肝脏功能学的指标ALT、AST及AKP的浓度明显降低,同时血清中抗炎因子IL-10和TGF-β含量升高,而促炎因子IL-6和TNF-α含量降低;使用JAK2/STAT3通路阻断剂AG490后,肝组织病理学改变明显,ALT、AST及AKP的浓度明显升高,IL-10和TGF-β含量降低,而IL-6和TNF-α含量明显升高。结论:白藜芦醇对肝脏缺血再灌注损伤的保护作用可能是通过激活JAK2/STAT3信号通路,改变再灌注过程中炎性反应实现的。  相似文献   

18.
曾剑儿 《甘肃医药》2014,33(1):21-22
目的:观察长期家庭氧疗(LTDOT)对慢性阻塞性肺病(COPD)患者血清中细胞因子及免疫球蛋白的影响.方法:将在我院门诊长期随访的40例COPD患者随机分为两组,常规治疗相同,治疗组于缓解期进行长期家庭氧疗,对照组于缓解期无氧疗.两组患者分别于治疗前及治疗1年后测定血清中细胞因子及免疫球蛋白浓度.结果:COPD患者长期家庭氧疗后血清中细胞因子TGF-β、TNF-α浓度非常显著低于对照组(P<0.01),IFN-γ、IL-4浓度明显低于对照组(P<0.05),免疫球蛋白浓度明显高于对照组(P<0.05).结论:COPD患者长期家庭氧疗可减轻全身性炎症反应,增强机体免疫力.  相似文献   

19.
目的::检测帕金森病( PD)患者血清活化T细胞趋化因子( RANTES)水平及炎症因子白细胞介素( IL)-1β、IL-6、肿瘤坏死因子( TNF)-α的变化,探讨其在PD免疫异常发病机制中的作用。方法:采用ELISA法检测60例PD患者( PD组)和35名健康体检者(对照组)血清RANTES、IL-1β、IL-6、TNF-α水平,采用Hoehn-Yahr、UPDRS量表分别对PD患者进行病情评估,同时对2组年龄、性别、吸烟、饮酒、血糖、血压、血脂差异一般状况进行检查。结果:PD组患者血清RANTES、IL-1β、IL-6和TNF-α水平均显著高于对照组(P<0.01);PD 1~5期患者血清RANTES水平差异均有统计学意义(P<0.05~P<0.01),而1~5期患者血清IL-1β、IL-6和TNF-α水平差异均无统计学意义(P>0.05);PD患者UPDR评分组间血清RANTES水平轻、中和重度组差异均有统计学意义(P<0.01),而IL-1β、IL-6和TNF-α水平轻、中和重度各组间差异均无统计学意义(P>0.05),且PD组血清RANTES水平与UPDRS评分呈显著正相关关系(P<0.01)。结论:PD患者血清RANTES、IL-1β、IL-6、TNF-α水平均较对照组有显著升高,提示炎症因子可能发动并维持了PD的发病进程,可为PD患者早期发现、防治提供优先依据。  相似文献   

20.
目的:观察儿童过敏性紫癜(HSP)患者血清白细胞介素-21(IL-21)、转化生长因子-β(TGF-β)、肿瘤坏死因子-α(TNF-α)和免疫球蛋白A1(IgA1)的变化及意义。方法收集我院儿科2013年6月至2015年8月收治的HSP急性期患儿200例,按照尿常规结果分为HSP组(n=109)和紫癜性肾炎(HSPN)组(n=91)。选取同时期在我院健康体检中心体检的20例健康儿童作为对照组。检测并分析三组儿童的血清IL-21、TGF-β、TNF-α、免疫球蛋白及补体水平变化,比较各组间IgA/C3值变化,并对HSP组患儿血清因子进行Pearson相关分析。结果 HSP组和HSPN组患儿的IL-21水平显著低于对照组,TGF-β、TNF-α、IgA1、IgA水平和IgA/C3显著高于对照组,且HSPN组TNF-α、IgA水平和IgA/C3显著高于HSP组,差异均有统计学意义(P>0.05或P<0.01),而IgG、IgM、C3和C4分别与对照组比较差异均无统计学意义(P>0.05);Pearson相关性分析结果显示,IL-21与TGF-β、TNF-α、IgA1、IgA及IgA/C3均无相关性(P>0.05),TGF-β与TNF-α呈正相关(P<0.05),IgA1与IgA、IgA/C3均呈正相关(P<0.05)。结论检测血清IL-21、TGF-β、TNF-α及IgA1有助于明确儿童过敏性紫癜的发生发展,及早采取措施,可防止病情继续恶化形成紫癜性肾炎。  相似文献   

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