可显影固体栓塞剂硫酸钡海藻酸钠微球的研制

目的:研制可显影固体栓塞剂硫酸钡海藻酸钠微球.方法:采用乳化-离子交联法制备微球,正交设计优化工艺,并考察微球的粒径、形态、分布、稳定性、悬浮性、包封率及X线显影性等理化特性.结果:微球平均粒径为(53.08±32.72) μm,分散良好,形态圆整;微球100℃水浴加热2 h、-4℃冰冻24 h、37℃振摇24 h、钴60(10 kGy)照射0.5 h各组的破损率分别为(2.0±1.1)%、(86.0±19.2)%、(39.0±14.7)%和(10.3±3.2)%;0.25%海藻酸钠微球混悬液的沉降体积比为(0.92±0.018);硫酸钡投料量为2.0 g时包封率为(69.2±13.2)%,且显影效果佳.结论:海藻酸钠、壳聚糖可作为硫酸钡微球的包封材料,乳化-离子交联法适用于此微球的制备.     

不透X射线栓塞微球的制备及其性质评价

目的：研究包载碘油的海藻酸钙微球（lipiodol-containing calcium alginate microspheres,LAMs）的制备方法、理化性质、栓塞性能和不透X射线的效果。方法：采用滴制法制备LAMs。通过正交实验考察乳剂处方中碘油与水相的比例、滴制操作中气流大小、砝码重量这3个因素对微球的数均粒径、粒径多分散性及碘油包封率的影响。以光学显微镜观察微球的形态,以物性测定仪考察微球的弹性,以视频旋转滴张力仪考察微球在导管内的推注性能,以X射线摄影系统考察微球在大鼠血管内的显影效果。结果：制备微球的最佳条件是碘油与水相的比例为3∶10,气流速度为40 g/mL,所用砝码质量为100 g。按正交实验优化工艺制备的微球,平均数均粒径为(493.9±42.6) μm,多分散性系数为1.02,碘油包封率为(88.97±1.09)%,微球圆整、表面光滑,微球变形60%时可承受的最大力平均值为(1.09±0.18)N,导管内推注性能良好。微球注入大鼠血管后,在X射线下显影效果清晰。结论：研制出的不透X射线微球外观圆整、粒径在栓塞所需范围内、弹性良好、导管内易推注、动物体内显影清晰,为介入栓塞治疗提供了一种新型的栓塞剂。     

二甲双胍缓释明胶微球的制备及其性质考察

制备二甲双胍缓释明胶微球并对其进行包衣,同时对其相关性质进行考察.以天然可生物降解的明胶为载体材料,液体石蜡为油相,司盘-80为乳化剂,用乳化-交联法制备二甲双胍明胶微球并考察了微球形态、粒径大小和分布、载药量和包封率等性质.此工艺制得的明胶微球粒径分布良好,包衣微球达到了缓释口服微球制剂学的要求.制备工艺简便、重现性好.     

可显影碘化油-氟尿嘧啶聚乳酸微球的研制

目的制备用于肝动脉栓塞的可显影碘化油-氟尿嘧啶(iodized oil-5-fluorouracil,iodized oil-5-Fu)聚乳酸(polylactide acid,PLA)微球。方法选择具有良好生物相容性及生物可降解性的聚乳酸为载体,加入阳性造影剂碘化油,采用复乳法制备可显影碘化油-氟尿嘧啶聚乳酸微球。以外观、粒径、载药量、包封率为考察指标,采用正交设计确定可显影碘化油-氟尿嘧啶聚乳酸微球的制备工艺。结果显微镜下微球球形圆整,粒径分布均匀,扫描电镜下微球表面有孔,平均粒径约100μm;载药量(10.78%±0.14%),包封率(63.34%±0.545%),微球显影效果好。结论以可生物降解的PLA材料可制备显影效果好的氟尿嘧啶微球,并且微球具有一定的缓释作用。     

正交法筛选莪术油微球制备工艺

目的通过正交设计法筛选出制备符合肝动脉栓塞要求的莪术油明胶微球的最佳工艺。方法以微球粒径、分布、栽药量、包封率等为指标,采用正交设计法优化微球的制备工艺。结果按优化工艺参数制备的微球外观形态良好,粒径符舍肝动脉栓塞的要求,平均载药量为9．45％,包封率为32．34％,平均收率为85,19％,重现性良好。结论确立的莪术油微球的制备工艺切实可行。      

正交设计优化吗啡明胶微球的制备工艺

目的通过正交设计筛选出吗啡明胶微球的最佳制备工艺。方法以微球粒径分布、载药量、包封率等为指标,采用正交设计法优化微球的制备工艺。结果微球形态良好,粒径符合要求,平均载药量为20．94％,包封率为86．27％,重现性良好。结论采用正交实验完成了吗啡明胶微球的制备工艺优化。     

阿奇霉素明胶微球制备工艺研究

目的对阿奇霉素明胶微球的制备工艺和包封率、粒径等特性进行初步研究。方法以生物降解材料明胶为载体,采用乳化聚合法制备阿奇霉素明胶微球,并采用正交试验优化制备工艺。结果所制备的阿奇霉素明胶微球外观圆整,平均粒径为58.34μm,载药量为20.11%,包封率为59.55%。结论所制备的阿奇霉素明胶微球重现性良好,粒径范围窄,载药量较高,制备工艺可行。     

白及微球的研制及其肝动脉栓塞实验研究

用乳化－冷凝技术首次制备出白及微球,并通过正交实验,确定了制备特定粒径微球的最佳工艺条件。对微球的外观、粒径与分布、溶胀度及血液相容性等进行了研究。微球平均粒径为５４．３６μｍ,粒径范围５０～９０μｍ,占总数的７８．３７％;微球在ｐＨ＝７．４的缓冲液中有一定的溶胀性;血液相溶性良好。实验小猪微球栓塞后的肝动脉造影表明白及微球可致肝右动脉一级、二级分支完全栓塞;病理检查表明梗死区可见肝组织呈肝硬化改变,大量的假小叶形成。微球栓塞后,ＧＰＴ有一过性升高,３周后恢复正常。实验结果表明,以白及胶为材料制备的微球将为癌症的治疗提供一种新型的栓塞治疗剂     

新型可显影复合栓塞微球的制备与评价

【立论依据】 我国肝癌发病率高,确诊时多为晚期,而且伴有严重的肝硬化,手术切除率低,迫切需要非手术治疗方法。化疗药物的疗效与肿瘤所在部位药物的有效血浓度及药物与肿瘤接触的时间呈正相关关系。而原发性肿瘤的血液供应90%~95%来自肝动脉,这就为TACE(transcatheterarter arterial chemoembolization)治疗肝癌提供了解剖学基础。肝癌的局部化疗使肝癌组织内药物浓度高出常规给药的10~30倍,全身副作用明显减少,疗效增加。如能与栓塞结合,使肝癌组织缺血,则两者之间有协同作用。因此,TACE是对不能手术切除的肝癌最有效的治疗方法之一。鉴于TACE疗法在现今临床的治疗比重逐步提升,栓塞化疗开始在肝癌治疗中普及,我们认为研发一款成本较为低廉、更宜被人体耐受且能自带显影功能的栓塞微球,能够在肝癌介入治疗的广泛普及中起到重要的作用。 【设计思路】 利用水相油相互不相溶的原理,选取价格低廉的生物医学上常用的胶质明胶和卡拉胶,以油-水-油结构制备可显影复合栓塞微球,并通过原位家兔肝癌模型评价栓塞微球的疗效。 【实验内容】 (1)制备结构为油-水-油/水,对应功能为稳定隔水-塑型载药-显影的两种带有不同显影剂和不同复合水溶胶的三相栓塞微球;(2)体外测定栓塞微球的性能:包括载药率、溶胀率、显影强度、生物利用率以及体外释药曲线;(3)建立原位家兔肝癌模型;(4)进行栓塞微球TACE试验,观察家兔肿瘤变化及存活率,进行体内栓塞微球自然降解时间、释药曲线、家兔对微球排异反应强度等的测定,并根据动物试验情况对栓塞微球的制备条件进行微调整。 【材料】 明胶、卡拉胶、石蜡、乳化剂、交联剂、脱水剂、碘化物显影剂。 【可行性】 (1)前期实验基础:已成功研制出油-水-油相微球,并且进行了多次载药率、溶胀率的测定,并将微球直径筛分成0~200 μm、200~300 μm、300~450 μm、450~1 200 μm四组微球。(2)实验平台:纳米生物纳米材料江苏省重点实验室。 【创新性】 本研究制备的具有自主知识产权新型可显影复合栓塞微球,成本低廉、自备显影功能、为人体可降解材料,具有一定的临床应用前景。     

阿霉素明胶微球的制备及体外释药特性

目的对阿霉素明胶微球(ADM-GMS)的制备工艺及体外释药特性进行研究.方法用乳化交联法制备阿霉素明胶微球,采用正交试验设计法考察影响制备工艺的各因素.用扫描电镜观察微球表面形态.并对阿霉素明胶微球的粒径大小及其分布、载药量、包封率、体外释药、稳定性等进行了测定.结果微球形态圆整,大小均匀、表面光滑,平均粒径为69.154μm,87.32%的微球粒径分布在50～120μm.微球平均载药量为2.13%,包封率为42.62%.微球体外降解和释药特性满足要求,且37℃下性质稳定.结论该制备工艺稳定,制得的阿霉素明胶微球有望成为临床用动脉栓塞术治疗癌症的新制剂.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.