乳腺导管癌淋巴转移拟态组织芯片的构建

目的 建立一套模拟乳腺导管癌淋巴结转移过程的组织芯片.方法 选用16例微小浸润性导管癌标本中的癌旁组织、导管原位癌和微小浸润癌组织;32例无淋巴结转移的浸润性导管癌标本中的非浸润性癌、浸润性癌和淋巴管癌栓以及32例淋巴结转移性浸润性导管癌标本中的非浸润性癌、浸润性癌、淋巴管癌栓和淋巴结转移性癌组织.按肿瘤发生发展的顺序制成组织芯片.常规石蜡切片,HE染色及免疫组织化学染色.结果 组织芯片所包含的信息和供体蜡块一致,组织芯片免疫组织化学染色结果与普通切片一致.结论 成功构建了乳腺导管癌淋巴结转移拟态组织芯片,为乳腺癌淋巴结转移机制的深入研究提供了良好的平台.     

Smad4和转化生长因子-β1在肝细胞癌组织芯片中的表达和意义

目的 研究Smad4和转化生长因子-β1(TGF-β1)在肝细胞癌组织芯片中的表达和意义.方法 使用组织芯片技术,选取200例肝细胞癌患者癌组织及癌旁肝组织,32例肝血管瘤患者瘤旁正常肝组织进行对照,分别进行Smad4和TGF-β1免疫组织化学染色,在显微镜下观察其表达的阳性率.结果 Smad4在对照组肝组织、癌旁肝组织、肝细胞癌中的表达呈逐渐下降的趋势,即Smad4在癌组织中表达有缺失,差异有统计学意义.在表达强度上,癌旁肝组织以强阳性表达为主,肝细胞癌中主要以弱阳性表达为主.TGF-β1在癌旁肝组织中的阳性表达率为96％,高于对照组,但在肝细胞癌中的阳性表达率降低为49.5％.对照组肝组织中的TGF-β1表达以弱阳性为主,占40.6％,在癌旁肝组织中以中度阳性为主,占51.5％,在肝细胞癌组,以阴性表达为主,占50.5％.由对照组到癌旁组织到肝细胞癌组,TGF-β1的表达呈现先升后降的趋势.结论 Smad4、TGF-β1与肝细胞癌的发生和肿瘤的生物学行为密切相关,TGF-β1可能作为肿瘤抑制因子和促进因子,发挥双向调节作用,缺失导致肝细胞癌发生,但过高表达可能使肿瘤细胞侵袭能力加强.     

精氨酸代谢酶ASS1/CAT1与结肠癌相关性研究

目的 探究精氨酸内源性合成酶精氨酸琥珀酸盐合成酶-1( argininosuccinate synthetase, ASS1)及外源性转运载体阳离子氯基酸转运蛋白-1(cationic amino acid transporter, CAT1)与结肠癌的相关性.方法 通过TCGA数据库和组织芯片探究非配对和配对样本中 ASS1/CAT1 在癌及癌旁组织中表达及与结肠癌相关性.结果 TCGA数据库分析提示非配对样本中结肠癌组织ASS1和CAT1表达均明显高于癌旁,ASS1在癌组织中的表达与TNM分期无关,CAT1的表达与TNM分期相关.组织芯片显示在配对样本中结肠癌组织及癌旁组织均有ASS1、CAT1表达.癌及癌旁组织ASS1表达无差异,CAT1则在癌组织表达明显高于癌旁组织(P=0. 000);癌组织ASS1、CAT1表达均与临床病理特征无关,但癌与癌旁组织ASS1表达量比值与淋巴转移正相关(P=0. 035).结论 结肠癌组织中,ASS1、CAT1均具有阳性表达,提示其同时存在精氨酸的内源性合成和外源性摄取途径.结肠癌淋巴转移和结肠癌组织及癌旁组织的ASS1表达比值正相关,但癌组织ASS1、CAT1表达与其他临床病理特征无关.结肠癌高表达CAT1,提示结肠癌精氨酸外源性摄取亢进.     

肝细胞癌中podoplanin的表达及其与淋巴道转移关系的研究

目的 观察肝细胞癌组织淋巴管内皮细胞podoplanin的表达,为研究肝细胞癌淋巴道转移的分子机制提供形态学依据.方法 用SP免疫组化检测法对肝细胞癌组织、淋巴结及正常肝组织中podoplanin的表达情况进行观察;用podoplanin来标记淋巴管,检测癌组织中微淋巴管密度.结果 肝细胞癌组织中血管内皮细胞podophnin不染色,淋巴管内皮细胞podoplanin阳性表达.经计数微淋巴管数量,癌组织和癌旁肝组织微淋巴管密度明显高于正常肝组织(P<0.01).结论 podophnin可选择性的在肝细胞癌组织淋巴管内皮表达,能明确的区分血管及淋巴管成分,可作为一个淋巴管内皮细胞较特异的标记物.由于微淋巴管密度在癌组织中的表达明显增高,说明淋巴管数量的增加可能与肝细胞癌的淋巴转移有关.     

结直肠癌瘤内以及血清IL-10和IL-12的检测及意义

目的 检测IL-10和IL-12在结直肠癌组织中含量及术前和术后3周血清浓度并探讨其意义.方法 采用ELISA法检测了46例结直肠癌IL-10和 IL-12在癌组织和癌周组织中含量及术前和术后1,3周血清浓度,以24例良性结肠息肉患者资料为对照.结果 结直肠癌组织IL-10含量高于癌周组织,而癌组织IL-12低于癌周组织;Ⅲ、Ⅳ期癌组织和癌周组织中IL-10含量高于Ⅰ、Ⅱ期,而Ⅰ、Ⅱ期癌组织和癌周组织中IL-12含量高于Ⅲ、Ⅳ.术前和术后1周血清IL-10升高而IL-12降低,术后3周血清IL-10和IL-12浓度接近对照组.瘤内以及血清IL-10和 IL-12呈明显负相关.结论 结直肠癌细胞可分泌IL-10,抑制 IL-12的产生,进而营造免疫抑制微环境.动态检测IL-10和 IL-12可反映结直肠癌病期、判断疗效和估计预后.     

细胞凋亡相关因子Caspase-3、Caspase-9和增殖细胞核抗原在人肾透明细胞癌中的表达

目的 检测人肾透明细胞癌组织中细胞凋亡相关重要因子Caspase-3、Caspase-9和增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)表达的变化,研究这些因子的表达与肾癌的关系.方法 标本离体后分成3组,①肾癌细胞组;②癌旁组织组;③正常肾组织组.均用10%福尔马林固定48 h,常规石蜡包埋、切片5 μm,应用免疫组织化学方法进行观察.结果 PCNA免疫组织化学染色结果,正常肾组织内的细胞核有弱的阳性表达,癌旁组织阳性表达增加,高于正常肾组织;肾癌组织阳性表达明显增强, 明显高于癌旁组织.Caspase-3与 Caspase-9均在正常肾组织中有弱的阳性反应,癌旁组织阳性表达增加,肾癌组织表达明显减少.3组之间两两比较差异均有统计学意义(P<0.01).肾癌4个期之间比较差异无统计学意义(P＞0.05).结论 癌旁组织Caspase-3和Caspase-9蛋白表达明显高于正常肾组织及癌组织,癌组织表达最少;癌组织中Caspase-3和Caspase-9表达下降,说明细胞凋亡减少;PCNA在正常肾组织组、癌旁组织组、肾癌组织组的表达呈明显加强趋势,癌组织细胞增殖旺盛,是肿瘤形成的重要机制.Caspase-3、Caspase-9和PCNA于肾透明细胞癌的表达在肿瘤分期间差异无统计学意义.Caspase-3与Caspase-9蛋白表达呈正相关.     

河南食管癌高发区食管癌组织中C-erbB-2蛋白和mRNA的表达

目的:探讨河南食管癌高发区居民食管癌组织中c-erbB-2 mRNA及蛋白的表达.方法:采用RT-PCR和免疫组化法检测食管癌高发区河南林州市正常人食管上皮组织和食管鳞癌手术切除的癌组织和癌旁组织中c-erbB-2的表达情况.结果:癌组织中C-erbB-2蛋白表达高于癌旁组织(P＜0.05);癌组织和癌旁组织中c-erbB-2mRNA表达率与正常人食管胃镜活检组织相比,差异无统计学意义.结论:C-erbB-2蛋白的表达在食管鳞癌的发生过程中可能起一定作用.     

大肠癌组织激光诱导自体荧光光谱规律

目的 探讨大肠癌组织激光诱导自体荧光(LIAF)光谱规律.方法 以Nd:YAG三倍频调Q激光器(波长380 nm)和光学多道分析仪(OMA)系统,自正常大肠组织、癌旁组织至癌组织,分别连续检测18例大肠癌手术标本的LIAF光谱.结果 大肠正常组织、癌旁组织和癌组织的LIAF光谱形状相似,均为双峰结构,主次峰波长分别为475 nm和550 nm;主峰强度由正常大肠组织经癌旁组织至癌组织依次为1413.78±96.46、751.22±76.32和490.84±81.28,癌组织平均降低922.94,与正常组织相比,差异有显著性(P＜0.001).结论 正常大肠组织、癌旁组织至癌组织LIAF光谱强度逐渐降低,光谱形状相似,主、次峰波长一致;癌组织的荧光强度低可能与激发光难于穿透癌组织激发到黏膜下层的荧光物质或(和)黏膜下层荧光物质减弱有关.     

人肾透明细胞癌组织中细胞增殖与凋亡及凋亡相关因子的表达

目的:本课题旨在通过检测人肾透明细胞癌组织中细胞增殖(PCNA)与凋亡(TUNEL法)及凋亡相关因子Caspase-3、Caspase-9表达的变化,研究这些因子的表达与肾癌的关系.方法:标本离体后分成三组,(Ⅰ)肾透明细胞癌组;(2)癌旁组织组;(3)证常肾组织组,应用HE染色法、TUNEL法及免疫组织化学方法进行观察研究.结果:(1)HE染色结粜:肾透明细胞癌组织镜下可见肿瘤细胞体积较大,圆形或多边形,胞质染色浅,透明或颗粒状,核同缩,间质富有毛细血管和血窦.(2)TUNEL检测结果:在肾癌组织、癌旁组织及正常肾组织组均有凋亡发生.凋亡肾组织细胞表现为染色质凝集、边集,核固缩.正常肾组织组细胞核有弱的阳性表达,癌旁组织表达明显增多,肾癌组织阳性表达最少.(3)PCNA免疫组化染色结果:正常肾组织内的细胞核有弱的阳性表达,癌旁阳性表达增加,肾癌组织阳性表达明显增多.(4)Caspase-3、Caspase-9免疫组化结果:Caspase_3 Caspase-9在难常肾组织中可见少量弱的阳性反应,癌旁组织阳性表达增加,肾癌组织表达明显减少.结论:癌旁细胞组织Caspase-3和Caspase-9的蛋白表达明显高于正常肾组织及癌组织,癌组织表达最少;而PCNA则相反,癌组织高于癌旁组织和正常肾组织,正常肾组织表达最少.癌组织中Caspase-3和Caspase-9表达下降,PCNA表达升高说明细胞凋亡减少,癌组织细胞增殖旺盛,是肿瘤形成的重要机制.Caspase-3、Caspase-9、PCNA于肾透明细胞癌的表达在肿瘤分期间无明显差异性.     

结肠癌组织中miR-451表达的检测与分析

目的 研究结肠癌组织和癌旁组织中miR-451的表达差异.方法 分别使用miRNA芯片和Real-time PCR检测结肠癌组织和癌旁组织中miR-451.结果 miRNA芯片检测发现结肠癌组织中miR-451相对于癌旁组织表达显著上调,并被Real-time PCR所证实.结论 结肠癌组织中miR-451表达上调.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.