Detection of hepatitis C virus RNA sequences in hepatic portal cholangiocarcinoma tisue by reverse t

ＨｅｐａｔｉｔｉｓＣｖｉｒｕｓ（ＨＣＶ）ｉｓａｐｏｓｉｔｉｖｅｓｔｒａｎｄＲＮＡｖｉｒｕｓａｓｏｃｉａｔｅｄｍｏｓｔｌｙｗｉｔｈｐｏｓｔｔｒａｎｓｆｕｓｉｏｎｎｏｎＡ，ｎｏｎＢｈｅｐａｔｉｔｉｓ．１ＰｅｒｓｉｓｔｅｎｔＨＣＶｉｎｆｅｃｔｉｏｎ...     

Nine Cases of Herpes Zoster with HIVInfection Treated with Rhubarb Powder

ＦｒｏｍＭａｙ１９９７ｔｏＭａｙ１９９８,ｗｈｉｌｅｔｈｅａｕｔｈｏｒｓｔｒｅａｔｅｄＨＩＶｉｎｆｅｃｔｅｄｐａｔｉｅｎｔｓｗｉｔｈｔｒａｄｉｔｉｏｎａｌＣｈｉｎｅｓｅｍｅｄｉｃｉｎｅ（ＴＣＭ）ｉｎＴａｎｚａｎｉａ,９ｃａｓｅｓｏｆｈｅｒｐｅｓｚｏｓｔｅｒｗｉｔｈＨＩＶｉｎｆｅｃｔｉｏｎｗｅｒｅｔｒｅａｔｅｄｗｉｔｈｔｈｅｐｏｗｄｅｒｏｆＲａｄｉｘｅｔＲｈｉｚｏｍａＲｈｅｉ（ＲＲＲ）．ＭＥＴＨＯＤＳＣｌｉｎｉｃａｌＤａｔａ　　ＮｉｎｅｃａｓｅｓｗｅｒｅｄｉａｇｎｏｓｅｄＨＩＶｐｏｓｉｔｉ…     

Prevention and therapy of hepatitis B

ＨｅｐａｔｉｔｉｓＢ（ＨＢ）ｉｓａｒａｔｈｅｒｈａｒｍｆｕｌｄｉｓｅａｓｅｔｏｔｈｅｈｕｍａｎｈｅａｌｔｈ－ＩｔｉｓｃａｕｓｅｄｂｙｈｅｐａｔｉｔｉｓＢｖｉｒｕｓ（ＨＢＶ）ｉｎｆｅｃｔｉｏｎ－ＰｅｏｐｌｅｉｎｆｅｃｔｅｄｗｉｔｈＨＢＶｗｉｌｌｎｏｔｏｎｌｙｄｅｖｅｌｏｐａｃｕｔｅｈｅｐａｔｉｔｉｓ,ｂｕｔａｌｓｏ２５％ｏｆｔｈｅｓｅＨＢＶｉｎｆｅｃｔｅｄｐａｔｉｅｎｔｓｗｉｌｌｔｅｎｄｔｏｓｕｆｆｅｒｆｒｏｍｃｈｒｏｎｉｃｈｅｐａｔｉｔｉｓ,ａｎｄｓｏｍｅｃｈｒｏｎｉｃｐａｔｉｅｎｔｓｅｖｅｎｃ…     

Prevalence of purified protein derivative positivity in human immunodeficiency virus infected individuals in Hong Kong

ＢｏｔｈｔｈｅＵＳＣｅｎｔｅｒｓｆｏｒＤｉｓｅａｓｅＣｏｎｔｒｏｌ (ＣＤＣ) 1　ａｎｄｔｈｅＷｏｒｌｄＨｅａｌｔｈＯｒｇａｎｉｚａｔｉｏｎ (ＷＨＯ )ｒｅｃｏｍｍｅｎｄｔｈａｔｉｓｏｎｉａｚｉｄｐｒｅｖｅｎｔｉｖｅｔｈｅｒａｐｙ (ＩＰＴ)ａｇａｉｎｓｔｔｕｂｅｒｃｕｌｏｓｉｓ (ＴＢ)ｂｅｒｏｕｔｉｎｅｌｙｇｉｖｅｎｔｏｈｕｍａｎｉｍｍｕｎｏｄｅｆｉｃｉｅｎｃｙｖｉｒｕｓ (ＨＩＶ)ｉｎｆｅｃｔｅｄｐｅｒｓｏｎｓｗｉｔｈｐｏｓｉｔｉｖｅｓｋｉｎｔｅｓｔｓｔｏｔｈｅｐｕｒｉｆｉｅｄｐｒｏｔｅｉｎｄｅｒｉｖａ…     

Clinical Study of Jinmaitong Composita(复方筋脉通) on Diabetic Peripheral Neuropathy

Ｄｉａｂｅｔｉｃｐｅｒｉｐｈｅｒａｌｎｅｕｒｏｐａｔｈｙ（ＤＮ）ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｃｏｍｍｏｎｄｉａｂｅｔｉｃｃｏｍｐｌｉｃａ ｔｉｏｎｓ．Ｉｔｓｐａｔｈｏｇｅｎｅｓｉｓｉｓｓｔｉｌｌｕｎｃｌｅａｒ,ｓｏｎｏｄｅｆｉｎｉｔｅｌｙｅｆｆｅｃｔｉｖｅｍｅｄｉｃｉｎｅｈａｓｂｅｅｎｆｏｕｎｄ．ＣｌｉｎｉｃａｌｓｙｍｐｔｏｍｓｏｆＤＮｃｏｕｌｄｂｅｓｉｇｎｉｆｉｃａｎｔｌｙｒｅｌｉｅｖｅｄｂｙＪｉｎｍａｉｔｏｎｇｃｏｍｐｏｓｉｔａ（ＪＭＴＣ,复方筋脉通）,ａｔｒａｄｉｔｉｏｎａｌＣｈｉｎｅｓｅ…     

Therapeutic Effect of Shengmai Injection(生脉注射液) on Respiratory Function in Chronic Obstructive Pulmonary Disease

Ｓｈｅｎｇｍａｉｉｎｊｅｃｔｉｏｎ（生脉注射液,ＳＭＩ）ｉｓｐｒｅｐａｒｅｄｏｎｔｈｅｂａｓｉｓｏｆａｔｒａｄｉｔｉｏｎａｌｃｌａｓｓｉｃｒｅｃｉｐｅ,ｗｈｉｃｈｃｏｕｌｄｓｔｒｅｎｇｔｈｅｎｄｉａｐｈｒａｇｍｃｏｎｔｒａｃｔｉｏｎ．（1）ＦｒｏｍＮｏｖ．1996ｔｏＡｕｇ．1997,ｔｈｅａｕｔｈｏｒｓｔｒｅａｔｅｄ20ｐａｔｉｅｎｔｓｏｆｃｈｒｏｎｉｃｏｂｓｔｒｕｃｔｉｖｅｐｕｌｍｏｎａｒｙｄｉｓｅａｓｅ（ＣＯＰＤ）ｗｉｔｈＳＭＩａｎｄｔｈｅｅｆｆｅｃｔｏｆＳＭＩｏｎｔｈｅｉｒｒｅｓｐｉｒａｔｏｒｙｆ…     

Carbohydrates in Helicobacter pylori infection

ＣａｒｂｏｈｙｄｒａｔｅｓｉｎＨｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉｉｎｆｅｃｔｉｏｎＪｉｎＣｈｅｎｇ金城ＯｂｊｅｃｔｉｖｅＴｏｓｔｕｄｙｔｈｅｒｏｌｅｏｆｃａｒｂｏｈｙｄｒａｔｅｓｉｎＨｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ（Ｈ．ｐｙｌｏｒｉ）ｉｎｆｅｃ...     

Studies on Immunotherapy against Melanoma in MiceUsing IL2 Gene－Transfected B16 Melanoma VaccineCombined with Specific CTL Ce

ＳｔｕｄｉｅｓｏｎＩｍｍｕｎｏｔｈｅｒａｐｙａｇａｉｎｓｔＭｅｌａｎｏｍａｉｎ　ＭｉｃｅＵｓｉｎｇＩＬ２Ｇｅｎｅ－ＴｒａｎｓｆｅｃｔｅｄＢ１６ＭｅｌａｎｏｍａＶａｃｃｉｎｅＣｏｍｂｉｎｅｄｗｉｔｈＳｐｅｃｉｆｉｃＣＴＬＣｅｌｌｓＮｉｕＧｕｉｌｉａｎ；...     

Studies on Treatment of Psoriasis with Integrative Medicine

Ｐｓｏｒｉａｓｉｓｉｓａｃｏｍｍｏｎ，ｅａｓｙｔｏｒｅｃｕｒ，ｃｈｒｏｎｉｃｉｎ ｆｌａｍｍａｔｏｒｙｄｉｓｅａｓｅｏｆｔｈｅｓｋｉｎ．Ｔｈｅｅｘａｃｔｃａｕｓｅｏｆｉｔｉｓｓｔｉｌｌｕｎｋｎｏｗｎ，ａｎｄｔｈｅｒｅａｒｅｍａｎｙｈｙｐｏｔｈｅｓｅｓｆｏｒｉｔ，ｓｕｃｈａｓｈｅｒｅｄｉｔｙ，ｉｎｆｅｃｔｉｏｎ，ｍｅｔａｂｏｌｉｃｄｉｓｏｒｄｅｒ，ｅｎ ｄｏｃｒｉｎｅｉｎｆｌｕｅｎｃｅ，ｎｅｕｒｏ -ｐｓｙｃｈｉｃｆａｃｔｏｒｓ，ｉｍｍｕｎｏ ｌｏｇｉｃｄｉｓｏｒｄｅｒ．Ｔｒｅａｔｍｅｎｔｏｆｐｓｏｒ…     

Effects of Rapeseed Oil Fume on Phagocytosis of Leucocytes in Mice

ＥｆｆｅｃｔｓｏｆＲａｐｅｓｅｅｄＯｉｌＦｕｍｅｏｎＰｈａｇｏｃｙｔｏｓｉｓｏｆＬｅｕｃｏｃｙｔｅｓｉｎＭｉｃｅ￥ＣｈｅｎＹｏｎｇｊｕａｎ；ＣｈｅｎＹｕｌｉａｎ；ＷｅｎｇＮｉａｎｎｏｎｇ．ＡＣＴＡＡＣＡＤＥＭＩＡＥＭＥＤＩＣＩＮＡＥＮＡＮＪＩＮＧ．１...     

A comparative study on the therapeutic effect of Astragaloside () and Perindopril in treating CVB3-infected cardiomyocytes in rats) and Perindopril in treating CVB3-infected cardiomyocytes in rats

Objective: To compare the therapeutic effects of Astragaloside and Perindopril on myocardial sarco/endoplasmic reticulum Ca²⁺ -ATPase (SERCA) activity and the SERCA type 2 mRNA level in Coxsack-ievirus B₃ (CVB₃)-infected cardiomyocytes.Methods: Cultured cardiomyocytes of rats were divided into normal, model, Astragaloside and Perindopril groups. The model, Astragaloside and Perindopril groups were infected with CVB₃. Meanwhile, the Astragaloside and the Perindopril groups were treated with Astragaloside (10 μg/ml) and Perindopril (1.3 μg/ml) respectively. Cytopathic effect (CPE), cardiac troponin I (cTnI), the SERCA activity and mRNA level of the SERCA type 2 were observed after 96 hours.Results: The CPE and cTnI of model group were significantly higher than those of normal, Astragaloside and Perindopril groups (P< 0.01). The activity and the mRNA expression of myocardial SERCA of model group were significantly lower than those of normal, Astragaloside and Perindopril groups (P < 0.01-0.05). Compared with Astragaloside group, the CPE, cTnI of Perindopril group were higher and the activity and mRNA level of Perindopril group were lower. But there were no significant difference between the two groups (P >0.05).Conclusion: Astragaloside and Perindopril were able to reverse the down-regulations of cardiac SERCA activity and mRNA expression caused by virus infection to alleviate the cardiomyocyte injury. This study was supported by National Natural Science Foundation (96-906-02-13), Science Commission Social Development Foundation of Jian-gsu Province (BS97028 and BS98039) and Health Bureau of Jiangsu Province (H9701).     

肾上腺皮质激素对心肌炎小鼠肌钙蛋白Ⅰ的影响

目的:研究肾上腺皮质激素治疗病毒性心肌炎时血清肌钙蛋白I(cTnI)的变化。方法:对不同时期应用激素治疗的心肌炎小鼠采用化学发光法检测血清cTnI水平,并同正常对照组及病毒对照组比较,同时与心肌病理积分进行相关分析。结果:病毒性心肌炎小鼠血清cTnI显著升高,并与病理变化呈直线正相关(r=0.70)。病毒性心肌炎早期和中期使用肾上腺皮质激素可明显降低cTnI水平,中期使用肾上腺皮质激素疗效尤为明显。结论:中期应用激素治疗CVB3心肌炎小鼠可明显阻止炎细胞浸润心脏组织,降低血清cTnI水平,保护心肌细胞,控制心肌炎病情发展,改善预后。     

促红细胞生成素对大鼠心肌缺血再灌注损伤的保护机制

目的探讨促红细胞生成素（EPO）对大鼠缺血再灌注损伤心肌的影响和可能机制。方法将成年健康雄性SD大鼠随机分为假手术组、缺血再灌注组、EPO预处理组,各组再灌注2h后血清检测一氧化氮（NO）含量和内皮型NO合酶（eNOS）活性、血清心肌型肌酸激酶同工酶（CK-MB）和心肌肌钙蛋白（cTnI）水平和心肌肌浆网钙泵（SERCA）活性。结果与缺血再灌注组比较,EPO预处理组血清NO含量升高（P〈0.05）,eNOS活性明显增加（P〈0.05）;血清CK-MB和cTnI水平降低（P〈0.05）;SERCA活性升高（P〈0.05）。结论 EPO能增加NO水平、eNOS和SERCA活性,有效减轻缺血再灌注心肌损伤,其机制可能与保护内皮细胞、减轻细胞内钙超载有关。     

内源性硫化氢对柯萨奇病毒B3性心肌炎小鼠心肌内病毒复制的影响

目的观察内源性硫化氢（H2S）对柯萨奇病毒B3（CVB3）所致病毒性心肌炎（VMC）小鼠心肌内病毒复制的影响,探讨内源性H2S在VMC发病机理中的作用。方法雄性Balb／c小鼠通过腹腔接种CVB,复制VMC模型。75只雄性Balb／c小鼠随机分为5组（每组15只）：正常对照组、VMC组及胱硫醚-1-裂解酶（CSE）不可逆抑制剂-炔丙基甘氨酸（PAG）低、中、高剂量干预组（PAG给药采用皮下注射,PAG低、中、高剂量干预组的PAG剂量分别为：20．0、40．0、80．0mg·kg^-1,1次／d,共6次。PAG的首次给药为腹腔接种CVB,的同时皮下注射PAG）。于腹腔接种CVB,后第7天处死小鼠。用光镜观察心肌组织的病理变化;用分光光度法测定心肌组织的H2S含量;用酶免疫吸附法测定心肌组织的CSE活性及血清的心肌肌钙蛋白I（cTnI）含量;用逆转录．聚合酶链反应方法检测心肌组织CVB,mRNA表达。结果VMC组心肌组织的CSE活性、H2S含量明显低于正常对照组（P〈0．01）,心肌组织的病理积分、CVB,mRNA表达水平及血清的cTn I含量明显高于正常对照组（P〈0．01）;PAG低、中、高剂量3个干预组心肌组织的CSE活性、H2S含量明显低于VMC组（P〈0．01）,心肌组织的病理积分、CVB,mRNA表达水平及血清的cTn I含量明显高于VMC组（P〈0．01）。结论心肌内源性H2S能抑制CVB,在心肌内复制。     

Effects of astragaloside in treating myocardial injury and myocardial sarco/endoplasmic ca2+ -atpase of viral myocarditis mice

Objective: To investigate the effects on myocardial injury and sarcoplasmic reticulum (SR) Ca²⁺ - ATPase of viral myocarditis mice treated with Astragaloside (AS) and Astragalus Injection (AI).Methods: Viral myocarditis model was created by intraperitoneal inoculation with coxsackievirus B_3m (CVB_3m ) solution and were divided into model, AS, AI and normal control groups. The mortality, myocardial pathological changes, serum cardiac troponin I (cTnl) and the activity of myocardial Sarco/Endoplasmic Ca²⁺-ATPase (SER-CA) were observed.Results: The mortality of model was higher than that of the normal control (P = 0. 0042), AS and AI (P<0.05). The serum level of cTnl of model was significantly higher than that of the normal control (P<0.001), AS (P<0.025) and AI (P < 0.05). The myocardial necrosis and inflammatory changes of AS and AI groups were alleviated than that of model (P < 0.01). The activity of myocardial SERCA of model were significantly lower than that of normal control (P<0.001), AS (P<0.01) and AI (P < 0.05).Conclusions: AS and AI have some protecting effects on myocardial injury of viral myocarditis mice. AS is the effective component ofAstragalus membranaceus in treating viral myocarditis. One of the mechanisms ofAstragalus membranaceus and AS for viral myocarditis mice depriving of the myocardial injury may be due to improve the activity of myocardial SERCA in the mice.     

大蒜多糖对小鼠病毒性心肌炎的治疗作用

目的 :探讨大蒜多糖对实验性小鼠病毒性心肌炎的治疗作用。方法 :采用BALB C小鼠腹腔注射柯萨奇病毒B3(CVB3)建立实验性小鼠病毒性心肌炎模型。小鼠随机分为正常对照组、模型组、大蒜多糖组、黄芪组。注射病毒 1h后给药 ,连续 7d ,于第 8d处死存活鼠取材。观察小鼠存活率及心肌病理变化 ,测定血清心肌酶、丙二醛(MDA)、一氧化氮 (NO)含量和谷胱甘肽过氧化物酶 (GSH Px)活力、心肌组织中病毒滴度及诱导型一氧化氮合酶(iNOS)的表达。结果 :大蒜多糖治疗组与模型组相比小鼠存活率明显增高 ,心肌组织病理损伤减轻 ,血清心肌酶、MDA、NO含量显著降低 ,GSH Px活力升高 ,心肌组织中病毒滴度及iNOS表达降低。结论 :大蒜多糖对实验性小鼠病毒性心肌炎具有治疗作用     

miRNA-1在大鼠心搏骤停心肺复苏后心肌焦亡及损伤中的作用

目的：探讨微小RNA-1（miR-1）在大鼠心搏骤停/心肺复苏（CA/CPR）后心肌细胞焦亡与损伤中的作用。方法：心搏骤停/心肺复苏模型的建立和分组：SD大鼠按照随机数字表法分为心搏骤停复苏（CA）组和假手术对照（Sham）组。前者再分为5组：CA组,CA+antago miR-1组,CA+antago miR NC组,CA+ago miR-1组,CA+ago miR NC组,每组n=6。所有6组大鼠在自主循环恢复（ROSC）后6 h取心尖部心肌组织、血样待测。用Real-time PCR法检测各组心肌细胞中miR-1的mRNA水平,Western blot法检测各组心肌细胞中焦亡相关的蛋白NLRP3、Caspase-1、IL-1β、IL-18的表达情况,用TUNEL染色技术检测心肌细胞焦亡率,ELISA技术检测血清中CK-MB、cTnI浓度。结果：各组大鼠体质量、基础平均动脉压（MAP）差异无统计学意义（P＞0.05）。与Sham组比较,CA组大鼠miR-1表达水平、心肌焦亡相关蛋白NLRP3、Caspase-1、IL-1β、IL-18表达、焦亡率、血清CK-MB与cTnI浓度升高（P＜0.05）。与CA组比,CA+antago miR-1组大鼠miR-1表达水平、心肌焦亡相关蛋白NLRP3、Caspase-1、IL-1β、IL-18表达、焦亡率、血清CK-MB与cTnI浓度降低（P＜0.05）,CA+ago miR-1组大鼠miR-1表达水平、心肌焦亡相关蛋白NLRP3、Caspase-1、IL-1β、IL-18表达、焦亡率、血清CK-MB与cTnI浓度升高（P＜0.05）,CA+antago miR NC组与CA+ago miR NC组大鼠miR-1表达水平、心肌焦亡相关蛋白NLRP3、Caspase-1、IL-1β、IL-18表达、焦亡率、血清CK-MB与cTnI浓度差异无统计学意义（P＞0.05）。结论：CA/CPR大鼠ROSC后存在明显心肌焦亡和心肌损伤,静脉使用ago miR-1和antago miR-1可以调节CA/CPR后大鼠心肌miR-1的表达,miR-1促进大鼠CA/CPR后心肌焦亡和心肌损伤,miR-1调节大鼠CA/CPR后心肌损伤机制可能和其促进心肌细胞焦亡相关。     

硫化氢对脓毒症大鼠心肌损伤的作用及其机制探讨

目的 研究硫化氢（hydrogen sulfide,H₂S）对脓毒症大鼠心肌损伤的作用,并探讨其作用的可能机制。 方法 采用盲肠结扎穿孔法制备大鼠脓毒症（cecal ligation and puncture,CLP）模型,将SD大鼠随机分为6组:假手术组、假手术+外源性H₂S供体硫氢化钠（NaHS）组、假手术+H₂S合成酶胱硫醚-γ-裂解酶（cystathionine-γ-lyase,CSE）抑制剂炔丙基甘氨酸（propargylglycine,PAG）组、CLP组、CLP+NaHS组、CLP+PAG组,每组各24只大鼠。分别于术后6 h、12 h、24 h处死各组大鼠（即各组分6 h、12 h、24 h亚组）取血和心肌标本,检测血清肌钙蛋白I（cTnI）水平,大鼠心肌组织HE染色观察病理变化,测定心肌组织肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）含量,采用RT-PCR方法检测心肌组织CSE mRNA表达,Western blot检测大鼠心肌组织核转录因子NF-κB表达。 结果 假手术各组及不同时点各指标差异均无统计学意义。与假手术12 h及24 h组相比,CLP 12 h及24 h组血清cTnI质量浓度以及心肌组织病理评分、心肌组织CSE mRNA、NF-κB表达、TNF-α及IL-10含量均升高（P均<0.05）;与CLP 12 h及24 h组相比,CLP+NaHS 12 h及24 h组血清cTnI质量浓度以及心肌组织病理评分、NF-κB表达和TNF-α含量降低（P均<0.05）,CSE mRNA表达和IL-10含量升高（P均<0.05）;而CLP+PAG 12 h及24 h组血清cTnI质量浓度及心肌组织病理评分、NF-κB表达和TNF-α含量升高（P均<0.05）,CSE mRNA表达和IL-10含量降低（P均<0.05）。 结论 H₂S在脓毒症所致的心肌损伤中起保护作用,这种保护作用的机制可能是通过抑制心肌组织中NF-κB表达、降低心肌组织TNF-α含量和提高心肌组织CSE mRNA表达、IL-10含量而保护心肌组织。     

白芍总苷对柯萨奇B3病毒感染大鼠原代心肌细胞的血清药理学干预

目的探讨白芍总苷（TGP）对柯萨奇B3病毒（CVB3）感染大鼠原代心肌细胞有无保护作用。方法取SD乳鼠心室肌制备大鼠原代心肌细胞,培养72h后,用不同剂量的TGP含药血清干预100 TCID50 CVB3感染心肌细胞,对比分析干扰素组、双黄连组、正常对照组。观察细胞病变,24h后测定细胞上清液中的cTnⅠ、CK、LDH以及用MTT法检测细胞存活率。结果TGP高剂量组可减轻心肌细胞病变,减少cTnⅠ、CK、LDH的释放。结论TGP对CVB3感染大鼠原代心肌细胞有保护作用。     

B组柯萨奇病毒对心肌细胞感染模式的研究

目的观察B组柯萨奇病毒（Coxsackie virusB,CVB）在原代心肌细胞中的感染特点,从而研究CVB对心肌细胞的致病机制。方法原代培养Balb／c乳鼠心肌细胞,并获得纯化的心肌细胞。使用B组柯萨奇病毒嗜心肌毒株3型（CVB3m）攻击原代心肌细胞,观察病毒感染心肌细胞后心肌细胞的细胞病变、超微结构和心肌酶的变化。结果成功原代培养了Balb／c乳鼠心肌细胞。心肌细胞在病毒感染后,细胞病变（CPE）不明显,与在HeLa等细胞中的典型CPE不同。心肌细胞在CVB3m感染后保持细胞形态和博动超过2周时间。电镜显示心肌细胞结构正常,细胞器数量增加,在心肌细胞胞质中可见晶格样病毒颗粒排列。培养细胞的上清液中心肌酶升高。结论CVB在原代心肌细胞中表现为持续感染,CVB在心肌细胞中的致病机制不同于HeLa细胞。