古钩藤水提液对小鼠实验性高血糖影响的实验研究

目的探讨古钩藤水提液对小鼠实验性高血糖的影响。方法建立链脲佐菌素和四氧嘧啶诱导小鼠高血糖模型,按高、中、低剂量的古钩藤水提液灌胃给药,空白对照组和模型对照组给予等体积的生理盐水,阳性对照组给予盐酸二甲双胍、格列本脲,观察给药后小鼠血糖的变化。结果给药前模型对照组、格列本脲组、古钩藤高剂量组、中剂量组、低剂量组5组之间血糖值比较,差异无统计学意义（P＞0．05）;给药后古钩藤高剂量组、中剂量组、低剂量组血糖值均明显低于链脲佐菌素模型对照组（P＜0．05）。给药前模型对照组、二甲双胍组,以及古钩藤高剂量组、中剂量组、低剂量组5组之间血糖值比较,差异无统计学意义（P＞0．05）;给药后古钩藤高剂量组、中剂量组、低剂量组血糖值均明显低于四氧嘧啶模型对照组（P＜0．05）。结论古钩藤水提液对链脲佐菌素致高血糖小鼠和四氧嘧啶致高血糖小鼠都有显著降血糖作用。     

糖尿病大鼠肝脏过氧化物增殖体受体γ辅激活子-1的表达及其意义

目的 探讨糖尿病肝脏过氧化物增殖体受体γ辅激活子-1(PGC-1)与糖尿病及高血糖的关系.方法 将大鼠分为3组:链脲佐菌素诱导的糖尿病大鼠11只为糖尿病组(A组);链脲佐菌素诱导的糖尿病大鼠经胰岛素治疗6只为胰岛素治疗组(B组);正常雄性Wistar大鼠9只为对照组(C组).测定大鼠肝脏PGC-1蛋白及mRNA的表达.同时测定血脂、空腹血糖(FBG)水平.结果 链脲佐菌素诱导的糖尿病大鼠FBG明显高于正常组大鼠(P<0.01).链脲佐菌素诱导出糖尿病后,其肝脏PGC.1蛋白表达高于正常大鼠(P相似文献   

岩藻多糖对2型糖尿病的预防作用

目的研究岩藻多糖对链脲佐菌素诱导实验性2型糖尿病大鼠的预防作用。方法建立2型糖尿病大鼠动物模型,预防给药岩藻多糖（600mg/kg、400mg/kg、200mg/kg）5周后,测定大鼠、空腹血糖、血脂和血清胰岛素水平。结果高脂高糖饮食联合腹腔注射小剂量链脲佐菌素（30 mg/kg）可诱导大鼠2型糖尿病模型。岩藻多糖可降低大鼠空腹血糖,提高基础胰岛素水平,升高胰岛素敏感指数,改善胰岛素抵抗。并能降低血清胆固醇、甘油三酯、低密度脂蛋白胆固醇含量,升高高密度脂蛋白胆固醇水平。结论岩藻多糖对实验性2型糖尿病的发生有预防作用。     

当归多糖对STZ诱导的糖尿病大鼠的降血糖作用及其机制

目的研究当归多糖(angelica polysaccharide,AP)对实验性高血糖大鼠血糖、胰岛素水平、体重、饮水量、饮食量和尿量的影响,旨在研究当归多糖对糖尿病大鼠降糖和改善临床症状的作用效果,探讨发现治疗糖尿病的新途径。方法链脲佐菌素(Streptozotocin,STZ)诱导的糖尿病Wister大鼠50只(体重200±20g)随机分成5组,分别用生理盐水、低剂量(20mg/kg)AP、中剂量(60mg/kg)AP和高剂量(200mg/kg)AP以及盐酸二甲双胍(150mg/kg)灌胃给药,给药周期为28天。同时采用正常的Wister大鼠为健康对照,观察AP对糖尿病大鼠的降糖作用,同时在给药0、7、14、21、28天测定体重和饮水量、饮食量和尿量,考察AP对改善糖尿病临床症状的影响。结果与模型组相比AP有明显的降血糖作用(P<0.05),呈现时间和剂量依赖关系,且给药4周时AP高剂量组的降糖效果与阳性药物对照组相当,没有统计学差异;AP给药2周后糖尿病的“三多一少”症状明显改善,且高剂量组与二甲双胍组相当;然而给药各组的胰岛素水平在实验前后没有统计学变化。结论AP能明显的降低Wister糖尿病大鼠的血糖和改善糖尿病的临床症状,但是其作用机制并不是通过增加胰岛素的浓度。     

藏药降糖舒胶囊降血糖作用的实验研究

目的:观察藏药降糖舒胶囊对多种动物模型的降血糖作用。方法:采用肾上腺素引起的高血糖小鼠模型,测定各模型小鼠的血糖水平;采用一次性腹腔注射链脲佐菌素的方法复制大鼠糖尿病模型,用降糖舒胶囊连续治疗7d。结果:藏药降糖舒胶囊对肾上腺素引起的高血糖小鼠也有明显的降血糖作用;对链脲佐菌素所致的糖尿病大鼠降糖舒胶囊高、中、低剂量组和模型对照组比较血糖显著下降(P<0.01),MDA含量明显降低(P<0.05),血清SOD水平、血清INS水平显著升高(P<0.05)。结论:藏药降糖舒胶囊对多种动物模型具有较好的降血糖作用。     

不同剂量链脲佐菌素建立妊娠糖尿病小鼠模型稳定性比较

目的探讨链脲佐菌素方法建立妊娠糖尿病小鼠模型中不同给药剂量对成模率及稳定性的影响。方法将40只C57BL/6J孕鼠随机分为对照组（10只）和实验组（链脲佐菌素低、中、高剂量组,每组10只）,对照组一次性腹腔注射等剂量的柠檬酸缓冲液,实验组分别予一次性腹腔注射现配链脲佐菌素溶液20 mg/kg、50 mg/kg、100 mg/kg,之后均给予普通饲料喂养,自由进水。分别于妊娠第3、10、18天测空腹血糖与体质量,并观察饮水量与尿量变化,比较孕鼠成模情况。结果 20 mg/kg STZ注射组成模率为40%,50 mg/kg链脲佐菌素组成模率为70%,100 mg/kg STZ注射组成模率为50%。空腹血糖与对照组相比较其高血糖状态持续时间长,且体质量明显下降,差异具有统计学意义（P〈0.05）。结论链脲佐菌素50 mg/kg腹腔注射C57BL/6J孕鼠是建立妊娠糖尿病小鼠模型的最佳剂量,具有较好的稳定性。     

链脲佐菌素诱导制备糖尿病大鼠模型方法学考察

目的 对腹腔注射链脲佐菌素制备糖尿病大鼠模型的方法学进行考察.方法 健康雄性Wistax大鼠,按60 mg/kg剂量一次性腹腔注射链脲佐菌素溶液,72 h后,断尾采集空腹静脉血,用葡萄糖氧化酶法测定血糖.结果 一次性腹腔注射链脲佐菌素制备糖尿病大鼠模型成功率达到75%,其血糖平均值为(19.46±2.53)mmol/L.模型大鼠血糖值在14 d内RSD小于1129%.结论 链脲佐菌素建立糖尿病动物模型过程中动物死亡率低、造模成功率高、稳定性好,是目前较好的一种建立糖尿病模型的药物.     

两种方法建立的妊娠期糖尿病小鼠模型

目的比较链脲佐菌素一次高剂量注射法与多次低剂量注射法建立妊娠期糖尿病小鼠模型的成模率及稳定性。方法将30只C57孕鼠随机分为对照组(l0只)和实验组(STZ 50mg/kg组、30mg/kg组,每组10只)。对照组一次性腹腔注射等剂量的柠檬酸缓冲液,实验组分别予一次性腹腔注射现配链脲佐菌素溶液50mg/kg和30mg/kg间隔24h腹腔注射,共注射3次。之后均给予普通饲料喂养,自由进水。分别于妊娠第0、6、9、19天测空腹血糖与体重,并观察饮水量与尿量变化,比较孕鼠成模情况。结果 50mg/kg链脲佐菌素组成模率为50%,30mg/kg组成膜率70%,空腹血糖与对照组相比较其高血糖状态持续时间长,且体重增加明显下降,差异具有统计学意义(P0.05)。结论链脲佐菌素30mg/kg,间隔24h腹腔注射,共注射3次是建立妊娠期糖尿病小鼠模型相对较好的方法,具有较好的稳定性。     

高脂喂养联合低剂量链脲佐菌素诱导糖尿病大鼠模型建立及其代谢特征分析

目的:建立高脂喂养联合低剂量链脲佐菌素(STZ)诱导的2型糖尿病大鼠模型并探讨其代谢特征。方法:35只6～7周龄雄性Wistar大鼠随机分为两组。正常对照组(NC)喂以基础饲料,糖尿病组(DM)喂以高脂高热量饲料,5周后给予DM组一次性腹腔注射STZ(30mg/kg),1周后测血糖,以空腹血糖>11.1mmol/L,并出现多饮、多尿等症状为成模标准。成模大鼠共15只,继续喂以高脂饮食。17周末处死所有大鼠,收集标本,记录体重、肝重,测定空腹血糖(FBG)、甘油三酯(TG)、总胆固醇(TC)、糖化血红蛋白(HbA1c)、糖化血清蛋白(GSP)等生化指标,放免法测定血清肿瘤坏死因子-α(TNF-α),胰岛素浓度(FIN),计算胰岛素敏感指数(ISI)及Homa胰岛素抵抗指数(Homa-IR)。结果:采用高脂喂养联合低剂量STZ一次性腹腔注射可以制作糖尿病大鼠模型,成功率75%,死亡率25%,该模型FBG、HbA1c、TG、TC均显著高于正常对照组,而胰岛素敏感性显著低于正常对照组。结论:高脂喂养联合低剂量链脲佐菌素注射的糖尿病大鼠模型可以模拟2型糖尿病发生的病理生理过程,具有高血糖、胰岛素敏感性下降及血脂异常等代谢特征。     

红景天乙醇提取液对实验性糖尿病大鼠血糖及肾脏结构变化的影响

[目的]研究长白山红景天乙醇提取液对实验性糖尿病大鼠血糖变化及肾脏结构改变的保护作用.[方法]取Wistar雄性大鼠,腹腔注射链脲佐菌素诱导制作糖尿病大鼠模型,随机分为模型对照组、红景天提取液大、中、小剂量组,另设正常对照组.分别灌胃给予红景天提取液大、中、小剂量组大鼠5.00,2.50,1.25g/kg红景天乙醇提取液,正常对照组及模型对照组大鼠分别灌胃给予等体积的蒸馏水,每日1次,周期为5周.末次给药后检测各组大鼠血糖,剖取肾脏,观察肾组织结构.[结果]红景天提取液各剂量组大鼠给药后血糖均显著低于给药前;肾脏组织化学检查结果显示,红景天提取液治疗5周后肾小球基底膜增厚得到明显改善.[结论]长白山红景天乙醇提取液对链脲佐菌素所致实验性糖尿病大鼠血糖升高及肾脏结构改变具有保护作用.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.