天然DNA和细胞因子对系统性红斑狼疮患者淋巴细胞体外分泌抗双链DNA抗体的影响

检测６例活动期、６例缓解期系统性红斑狼疮（ＳＬＥ）患者和１０例正常人血清和外周血单个核细胞（ＦＢＭＣ）自发及经天然ＤＮＡ（ｎＤＮＡ）、重组白介素２（ｒＩＬ－２）和重组γ干扰素（ｒＩＦＮ－γ）锈导后分泌ＩｇＧ类抗双链ＤＮＡ（ｄｓＤＮＡ）特异性抗体水平，观察到活动期患者血清中含量和自发分泌量显著高于缓解期，后者又显著高于正常人，且体外细胞分泌水平与血清中含量密切相关（ｒ＝０．９８８２）。ｎＤＮＡ能特异地激发ＳＬＢ患者的ＰＢＭＸＣ分泌抗ｄｓＤＮＡ抗体，但对正常人无作用。ｒＩＬ－２和ｒＩＦＮ－γ皆能增强ＳＬＥ患者ＰＢＭＣ产生抗ｄｅＤＮＡ抗体。ｎＤＮＡ和ｒＩＬ－２联合应用时对活动期患者ＰＢＭＣ分泌抗ｄｓＤＮＡ抗体有协同作用。作者未发现重组白介素６（ｒＩＬ－６）对抗ｄｓＤＮＡ抗体分泌的影响。     

EFFECT OF HYPOXIA ON DNA SYNTHESIS AND C－MYC GENE EXPRESSION OF PULMONARY ARTERY SMOOTH MUSCLE CELLS

ＥＦＦＥＣＴＯＦＨＹＰＯＸＩＡＯＮＤＮＡＳＹＮＴＨＥＳＩＳＡＮＤＣ－ＭＹＣＧＥＮＥＥＸＰＲＥＳＳＩＯＮＯＦＰＵＬＭＯＮＡＲＹＡＲＴＥＲＹＳＭＯＯＴＨＭＵＳＣＬＥＣＥＬＬＳＬｕｏＬａｎ（罗兰）；ＬｉＳｈｉｑｉａｎｇ（李世强）ａｎｄＣａｉＹｉｎｇｎｉａｎ...     

IL—12对新生IFN—γ产生的影响

白细胞介素１２（ＩＬ－１２）是一种重要的细胞因子，由单核／巨噬细胞，Ｂ细胞产生，诱导Ｔ细胞和ＮＫ细胞产生γ干扰素（ＩＦＮ－γ）在细胞免疫反应发挥多种作用，新生儿产生ＩＬ－１２的能力鲜见报道，本用逆转录－聚合酶链式反应（ＲＴ－ＰＣＲ）方法检测８例新生儿脐血单个核细胞（ＣＢＭＣ）ＩＬ－１２Ｐ４０ｍＲＮＡ和ＩＦＮ－γｍＲＮＡ表达，及ＥＬＩＳＡ法新生儿ＣＢＭＣ培养上清ＩＦＮ－γ浓度；并观察重组ＩＬ－１２     

DDPH对缺氧内皮细胞条件培养液致肺动脉平滑肌细胞PDGF mRNA表达的影响

利用地高辛标记的ｃＤＮＡ探针，原位检测ＤＤＰＨ［１－（２，６－二甲基苯氧基）－２－（３，４－二甲氧基苯乙胺基）丙烷酸盐］对缺氧内皮细胞条件培养液致猪ＰＡＳＭＣＰＤＧＦ基因表达的影响。实验分６组：常氧、低氧无血清培养液组（Ｎ、Ｈ组），常氧、低氧内皮细胞条件培养液组（ＮＥＣＣＭ、ＨＥＣＣＭ组），ＮＥＣＣＭ＋ＤＤＰＨ、ＨＥＣＣＭ＋ＤＤＰＨ组。用自动图像分析仪检测各组细胞ＰＤＧＦ－Ａ和－Ｂ链杂交产物的平均光密度（ＯＤ）值。结果：ＨＥＣＣＭ组ＰＤＧＦ－Ａ和－Ｂ链ｍＲＮＡ表达量分别是ＮＥＣＣＭ组的１．４０、１．４９倍（Ｐ＜０．０１），分别是Ｎ组的１．８倍、１．７倍（Ｐ＜０．０１），ＨＥＣＣＭ＋ＤＤＰＨ组ＰＤＧＦ－Ａ、－Ｂ链ｍＲＮＡ表达量分别为ＨＥＣＣＭ的０．７３倍、０．６５倍（Ｐ＜０．０１），与ＮＥＣＣＭ组相比，均无显著差异。提示ＤＤＰＨ在ＰＤＧＦ基因转录水平抑制ＨＥＣＣＭ诱导的ＰＡＳＭＣ的增殖。     

“急淋”白血病细胞与高内皮小静脉及骨髓基质细胞粘附行为的…

采用改进的小鼠肠系膜淋巴结（ＭＬＮ）冰冻切片粘附结合实验法和ＭＴＴ法，观察白血病患者尤其是Ｂ细胞急性淋巴细胞性白血病（Ｂ－ＡＬＬ）患者外周血单个核细胞（ＰＢＭＣ）体外粘附小鼠ＭＬＮ高内皮小静脉（ＨＥＶ）的活性变化及患者骨髓血单个核细胞（ＢＭＭＣ）体外粘附患者骨髓基质细胞（ＳＣ）的活性变化，结果：１Ｂ－ＡＬＬ患者ＰＢＭＣ粘会ＨＥＶ的阳性率和强阳性率均低于正常组；２．经抗淋巴细胞功能相关抗原－１（ＬＦ     

EXPRESSION OF HLA－CLASS Ⅱ GENES OF IDDM PATIENTS ON THE SURFACE OF THE LTK~－ CELLS

ＥＸＰＲＥＳＳＩＯＮＯＦＨＬＡ－ＣＬＡＳＳⅡＧＥＮＥＳＯＦＩＤＤＭＰＡＴＩＥＮＴＳＯＮＴＨＥＳＵＲＦＡＣＥＯＦＴＨＥＬＴＫ~－ＣＥＬＬＳ￥ＷａｎｇＨｅｎｇ（王姮）ａｎｄＳｕｎＱｉ（孙琦）（ＰＵＭＣＨｏｓｐｉｔａｌ，ＣＡＭＳ＆ＰＵＭＣ，Ｂｅｉｊｉｎｇ１?..     

THE ROLE OF RECOMBINANT HUMAN FUSION PROTEIN IL－6／IL－2（CH925） IN HEPADNA VIRUS INFECTION TREATMENT

ＴＨＥＲＯＬＥＯＦＲＥＣＯＭＢＩＮＡＮＴＨＵＭＡＮＦＵＳＩＯＮＰＲＯＴＥＩＮＩＬ－６／ＩＬ－２（ＣＨ９２５）ＩＮＨＥＰＡＤＮＡＶＩＲＵＳＩＮＦＥＣＴＩＯＮＴＲＥＡＴＭＥＮＴＭｉＺｈｉｂａｏ（米志宝），ＺｈａｏＣｈｕｎｈｕａ（赵春华），ＺｈａｎｇＸｉｔ...     

EFFECT OF CORDYCEPS SINENSIS PREPARATION(ZHI LING,ZL)ON BODY NITROGEN METABOLISM IN CHRONIC RENAL FAILURE

ＥＦＦＥＣＴＯＦＣＯＲＤＹＣＥＰＳＳＩＮＥＮＳＩＳＰＲＥＰＡＲＡＴＩＯＮ（ＺＨＩＬＩＮＧ，ＺＬ）ＯＮＢＯＤＹＮＩＴＲＯＧＥＮＭＥＴＡＢＯＬＩＳＭＩＮＣＨＲＯＮＩＣＲＥＮＡＬＦＡＩＬＵＲＥＺｈｕＨａｎｗｅｉ（朱汉威），ＺｕｏＪｉｎｇｎａｎ（左静南），Ｈ...     

“急淋”白血病细胞与高内皮小静脉及骨髓基质细胞粘附行为的变化

采用改进的小鼠肠系膜淋巴结（ＭＬＮ）冰冻切片粘附结合实验法和ＭＴＴ法，观察白血病患者尤其是Ｂ细胞性急性淋巴细胞性白血病（Ｂ—ＡＬＬ）患者外周血单个核细胞（ＰＢＭＣ）体外粘附小鼠ＭＬＮ高内皮小静脉（ＨＥＶ）的活性变化及患者骨髓班单个核细胞（ＢＭＭＣ）体外粘附患者骨髓基质细胞（ＳＣ）的活性变化．结果：１．Ｂ—ＡＬＬ，患者ＰＢＭＣ粘附ＨＥＶ的阳性率和强阳性率均低于正常组；２．经抗淋巴细胞功能相关抗原一１（ＬＦＡ—１）单克隆抗体（ＭｃＡｂ）封闭后，Ｂ—ＡＬＬ患者ＰＢＭＣ对ＨＥＶ的粘附阳性率低于正常对照ＰＢＭＣ经同样处理组及自身ＰＢＭＣ末处理组。抗ＬＦＡ一１ＭｃＡａｂ封闭也能使正常对照ＰＢＭＣ粘附阳性率和强阳性率下降；３．各型ＡＬＬ患者ＢＭＭＣ与患者骨成ＳＣ的粘附率低于正常对照，各型患者组问没有显著差异。     

哮喘患儿自然杀伤细胞及γ—干扰素水平的变化

目的：探讨哮喘患儿自然杀伤细胞及γ－干扰素水平的变化及意义。方法：应用四甲基偶氮唑盐（ＭＴＴ）比色法、流式细胞术（ＦＣＭ）及生物素－亲合素双抗体夹心酶联免疫吸附分析术（ＡＢＣ－ＥＬＩＳＡ）检测哮喘患儿外周血自然杀伤细胞（ＮＫＣ）活性、ＣＤ５６＾＋细胞数及单个核细胞（ＰＢＭＮＣ）诱生γ－干扰素（ＩＦＮ－γ）水平。结果：哮喘发作期及缓解期ＮＫＣ活性与正常对照比较均无显著差异（Ｐ〉０．０５）；发作期ＣＤ     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.