白川胶囊对清醒大鼠血压的影响

目的 观察白川胶囊的急慢性降压作用。方法 以间接测压法测量清醒大鼠尾动脉血压;①测量自发性高血压大鼠（SHR）多次灌胃白川胶囊的血压变化,观察其慢性降压作用;②测量肾性高血压大鼠1次灌胃白川胶囊后1、2、4、6、8h的血压值;观察其急性降压作用。结果 ①连续20d灌胃白川胶囊0.6或0.3g/kg均明显降低SHR的血压,白川胶囊0.15g/kg也有一定降压作用;②1次性灌胃白川胶囊0.6或0.3g/kg均明显降低肾性高血压大鼠血压,白川胶囊0.15g/kg也有降压作用。结论 白川胶囊有明显的急慢性降压作用。     

杜仲叶提取物对清醒大鼠血压的影响

目的观察杜仲叶提取物急慢性降压作用。方法以间接测压法测量清醒大鼠尾动脉血压：①测量自发性高血压大鼠（SHR）多次灌胃杜仲叶提取物的血压变化，观察其慢性降压作用；②测量肾性高血压大鼠1次灌胃杜仲提取物后1、2、4、6、8、h血压，以观察提取物急性降压作用。结果①连续18d灌胃杜仲叶提取物4．2g／kg或6．3g／kg均明显降低SHR血压；②1次性灌杜仲叶提取物4．2g／kg或6．3g／kg均明显降低肾性高血压大鼠血压。结论杜仲叶提取物有明显的降低急慢性血压作用。     

血灵的抗高血压实验研究

目的：证实血灵的抗高血压作用。方法：采用iv给药、一次ig给药和多次ig给药，观察血灵是自发性高血压大鼠（spontaneously hypertensive rat strain,SHR)血压及心率的影响；采用iv给药，观察血灵对Wister大鼠、犬正常血压的影响；通过降压作用快速耐受试验，观察血灵在短时间内连续静脉注射有无快速耐受现象。结果：血灵三个剂量组（0．25g/kg、0．3g/kg、0．50g/kg)iv给药或（0.5g/kg、1.0g/kg、2.0g/kg)ig给药，均能使SHR自发性高血压大鼠的血压显著下降，一次ig给药后1h血压已有下降，2h降压作用最强，维持时间约为6h；多次ig给药则至14d仍有降压作用；（0.02g/kg、0.04g/kg、0.1g/kg）iv给药能使犬的正常血压显著下降，且作用随着剂量的增大而增强，多次反复注射也不产生耐受性，并对心率无明显影响。结论：血灵具有显著的降血作用。     

妇血宁药理作用初探（Ⅱ）

妇血宁3mg/ml有兴奋大鼠离体子宫平滑肌作用,生物效价测定表明1mg妇血宁收缩大鼠和小鼠子宫肌的作用强度与5×10~(-5)u和1.8×10~(-5)u的垂体后叶素相当。2mg/ml妇血宁松弛豚鼠气管条和家兔回肠平滑肌,对蟾蜍心脏有兴奋作用。猫静脉注射妇血宁500mg/kg血压先升后降,心率加快,未见其致敏作用。     

甜菊甙抗高血压作用的实验研究

清醒血压正常WKY大鼠、自发性高血压大鼠、DOCA-NaCl和肾性高血压大鼠给予甜菊式50或100mg/kg,ip后可引起与剂量有关的收缩压和舒张压平行降低,除WKY大鼠50mg/kg组外,其余各组血压变化值和变化百分数与各盐水对照组对应值比差异均有显著性意义。同剂量甜菊甙ip后作用相比,药物在高血压大鼠的作用较正常血压夫鼠明显,给予WKY大鼠、SHR甜菊甙100或200mg/kg灌胃,SHR长期饮用甜菊式液均引起血压降低。收缩压与舒张压药后变化值的相关分析表明:甜菊甙的降压作用主要与舒张压降低有关,这在抗高血压作用中尤为重要。     

SHR,WKY大鼠心,肾及胸主动脉ACE,ACE2表达的差异与高血压的关系

目的观察SHR,WKY大鼠心,肾,胸主动脉及体外培养的VSMCs中的ACE,ACE2表达的差异,探讨ACE与ACE2在自发性高血压大鼠高血压形成的机制。方法选取6个月的雄性自发性高血压大鼠SHR(Spontanous Hypertensive Rat,SHR)及与之配对的正常大鼠WKY。尾袖法测定大鼠血压,断头取血,称量全心与左心重量,计算心体比与左室重指数。免疫组化法检测肾脏ACE,ACE2的表达。放免法测定血浆中AII水平。RT-PCR法比较SHR与WKY大鼠心,肾,胸主动及体外培养VSMC细胞ACE、ACE2 mRNA的表达水平。结果(1)6月龄SHR大鼠血压显著高于WKY(196.42±18.90,116.35±14.48,P<0.05),而WKY大鼠心体比及左室指数明显低于SHR(2.84±0.28,3.79±0.41,P<0.05;2.17±0.18,2.95±0.28,P<0.05),SHR大鼠血浆中AII水平明显增高(1675.35±305.16,694.38±112.56,P<0.05)。(2)肾脏ACE2免疫染色SHR明显低WKY(0.054±0.013,0.205±0.043,P<0.05)。与WKY相比,SHR心,肾,胸主动脉ACE2 mRNA表达水平分别减少49.84%4、0.67%3、6.17%,,而ACEmRNA表达水平分别增加33.66%、29.83%、42.16%。在体外培养的胸主动脉平滑肌细胞(thorax aorta vascularsmooth mulscle cell,TAVSMC)SHR大鼠ACE mRNA表达水平明显高于WKY,ACE2 mRNA的表达水平显著降低。结论(1)ACE与ACE2表达失调可能是高血压形成的重要原因。(2)ACE与ACE2表达差异可能大鼠遗传背景有关。     

自发性高血压大鼠肾血管收缩功能改变

观察14周及28周龄自发性高血压大鼠（SHR）及同基因正常血压WKY鼠离体灌注肾装置对去甲肾上腺素（NE）及氯化钡的升压反应，结果表明，SHR肾血砭呈超常收缩，剂量-压力曲线左移，且随周龄增加而明显；对氯化钡的升压反应时间明显延长而对NE反应时间无明显变化，揭示SHR肾血管平滑肌细胞清除氯化钡的能力下降。     

通心络对高血压、高血糖合并高胆固醇血症大鼠动脉的影响及机制

目的 评价通心络对高血压、高血糖合并高胆固醇血症大鼠模型动脉功能的影响及机制.方法 将体重300～400 g的成年自发性高血压大鼠(SHR)32只随机分为普食组(SHR组)、对照组(模型组)、低剂量通心络(0.2 g/kg)干预组(通心络低组)、高剂量通心络(0.8 g/kg)干预组(通心络高组),另取8只正常血压大鼠作为正常组,高脂喂养4周建立动物模型.通心络干预4周后,行主动脉离体血管功能检查,用荧光定量PCR方法测定过氧化物酶体增殖物受体γ(PPAR-γ)mRNA表达.结果 通心络干预后大鼠离体主动脉对乙酰胆碱、硝普钠引起的舒张反应增强,通心络高组对A23187的舒张反应增强(P＜0.05),通心络剂量依赖性增加PPAR-γ基因表达.结论 通心络干预具有保护血管内皮功能及逆转血管平滑肌功能障碍等作用,其机制可能与激活PPAR-γ基因表达有关.     

EMD56431对高血压大鼠的降压作用

胃的 探讨ATP敏感钾通道(KATP)开放剂EMD56431对清醒自发性高血压大鼠(SHR)和肾性高血压大鼠(RHR)血压的影响。方法 采用静脉注射和灌胃给药的方式，经颈总动脉插管，动态监测动脉收缩压(SAP)，舒张压(DAP)及心串(HR)的变化。并测定小鼠灌胃给予EMD56431时的半数致死剂量(LD50)。结果 静注或灌胃给予EMD56431，25、50、100μg/kg均可使SHR血压产生剂量依赖性的下降，同时反射性加快HR。前者降压维持时间分别为45min、6h和6h以上，后者可分别维持45min、lh和6h以上。静注和灌胃EMD56431产生的效应相当。灌胃EMD5643l亦可使RHR血压产生剂量依赖性的下降，降压维持时间分别为30min、45min和6h以上。小鼠LD50为8．54mg/kg，95％可信区限为7．87—9．93mg/kg。结论 EMD56431对SHR和RHR有显著而持久的降压作用，安全有效，且该药生物利用度较高，具有广阔的临床开发前景。     

清肝降压饮对自发性高血压大鼠心肌eNOS表达的影响

目的：观察清肝降压饮防治自发性高血压大鼠（SHR）心肌肥厚的作用,并探讨其作用机制。方法：将SHR随机分为模型组、清肝降压饮低剂量组、清肝降压饮高剂量组和西药组,并用Wistar大鼠作空白对照。灌胃4周后,利用免疫组化方法检测大鼠心肌内皮型一氧化氮合酶（eNOS）的表达。结果：各给药组大鼠血压、左室重量指数均较模型组显著降低（P〈0.01）,同时各给药组大鼠心肌细胞eNOS的表达均较模型组显著升高（P〈0.01）,其中清肝降压饮高剂量组效果最显著。结论：清肝降压饮可通过促进SHR心肌细胞eNOS的表达,降低SHR的血压和左心室重量指数,产生防治高血压心肌肥厚的效应。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.