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1.
目的:探讨自然周期成熟卵体外成熟培养(In vitromaturation,IVM)、受精和胚胎移植技术在治疗不育症中的应用.方法:选取30对接受体外授精/卵胞浆内单精子显微注射-胚胎移植(In vitrofertilization/intracytoplasmic sperminjection-embryotransfer,ⅣF/ICSI-ET)助孕的小育夫妇为对象,在自然月经周期卵泡直径>10 mm、内源性LH(Luteinizing hormone)峰出现之前采卵.采卵当日成熟卵(Metaphase Ⅱ,M Ⅱ)行IVF授精,未成熟卵进行体外培养24~48 h,成熟至M Ⅱ期后行ICSI授精,受精后72 h行胚胎移植.结果:30个ⅣM周期中,2个周期取卵末获卵.另28个周期共获卵113个,其中成熟卵29个,退化卵10个,未成熟卵74个,体外培养后成熟印个,成熟率81.08%.20个周期有胚胎移植,6例临床妊娠.其中5例成功分娩足月健康婴儿(1例来自成熟卵、4例来自未成熟卵),1例临床妊娠流产.结论:自然周期未成熟卵IVM、受精和胚胎移植技术可以用于治疗不育患者.  相似文献   

2.
对13头成年母猴,用黄体酮 前列腺索(PGF_(2a))处理,诱发月经周期,并用三种不同的处理方案,注射外激性促性腺激素诱发等超数排卵。对171个大滤泡(直径≥4mm)实行外科手术采卵,滤泡穿刺吸卵共获147个卵细胞,平均每头猴采卵11.31±6.47个,卵细胞回收率为85.96%。精液用直肠电刺激法采集,精子经TALP培养基洗涤二次,并在含10%灭活猴血清的TALP液中,加入1mM CAMP和lmM咖啡因,预孵育6~3h。卵细胞在含10%灭活猴血清的TALP或Ham''sF-10培养基中作体外成熟的培养,经约4~12h孵育,卵细胞体外成热率为28.57%。成熟的卵细胞(n=42,中期Ⅱ)和已预孵育的精子一起12~24h,并有部分在前次输精后5~12h再次输精,在42~56h后有9个受精卵继续发育至2细胞期和桑椹期阶段,体外受精率为35.71%(15/42)。  相似文献   

3.
生儿育女是家庭生活的一个重要组成部分,然而在所有结婚的夫妇中大约有10%的夫妇由于某种原因而无法获得小孩,为了解决这些人的烦恼,人工授精与体外授精技术便应运而生。 人工授精是用人工的方式将精子注入子宫内,促使女性怀孕;体外授精是以人工方式将取自母亲的卵子和取自父亲的精子先在试管内混合授精,之后再将授精卵移植到子宫内使它着床。两种授精方式相比,体外授精比人工授精在技术操作上要复杂得多,手术的第一步是排卵前卵子的吸取,在这个步骤中妇女必须全身麻醉,并由医生以腹腔镜在完全无菌的手术室中进行。第二步是将取出的数个卵子按不成熟  相似文献   

4.
虽然哺乳动物的精子可以在体外妥善保存,但尚不知这样的精子是否能在辅卵菅中受精,也不知道它们的受精能力是否与保存在体内的精子相同.本文用保存过的精子进行体外受精(方法前已报道,即在37℃,380mOsm/千克的高离子强度的培养剂HIS中置放20分钟),结果精子不能在输卵菅中渗入卵细胞,除非让精子在雌兔输卵管中有较长时间的适应过程.因此,尽管精子可能适应保存的环境,HIS处理显然是不能完成这一过程的.然而,精子在另一组限定条件下培养12小时(37℃,无牛血蛋白培养剂 20%在油下用5%CO_2与8%O_2平衡过的血清),将此种精子输入12——15小时后检查,在14次试验中,有7次使卵细胞受精.虽然如此,体外保存了12小时的精子,其授精能力(131个卵的29%受精;0.3卵周精子/卵(比在对侧输卵管中的子宫内精子要小10倍(43个卵的81%受精;15个卵周精子/卵).而且经过后一种处理过的精子,若与许多没有处理过  相似文献   

5.
倪丰  姜宏  裴红  朱杰 《安徽医学》2006,27(3):F0003-F0003
患者,女,29岁,4年前自然流产1次,因双侧输卵管梗阻入我中心行体外授精-胚胎移植术,采用长方案进行控制性超排,体外授精后培养2天,于2005年4月11日选择3个胚胎(8/Ⅰ×2,6/Ⅱ×1),在超声引导下于距宫底1cm处植入胚胎,术后平卧6小时。移植后15天查尿HCG( ),继续黄体支持治疗。12天  相似文献   

6.
950071人卵、受精那及前期狂胎染色体初步分析刘平一产中华妇产科杂志一19叫.2,(1如一功~·12 应用36例妇女进行体外授精一胚胎移值研究淘汰的未受精卵,未分裂的受精卯及形态不好的前期胚胎,制成染色体标本44个,进行染色休分析。结果:正常未受精卵标本13个.染色体数目正常.卵母细咆处于第二次减数分裂中期,体外授精失败,结合病史分析,与精子质量和卵成熟度有关;异常未受精标本4个,正常卵受精标本7个.染色体数目正常的卵母细胞虽受精,但有的未抛出第二极体,精卵发育不同步;异常卵受精标本8个,染色体数目异常的卵母细胞虽受精,但发育停滞,多精…  相似文献   

7.
目的 分析本中心开展体外授精 -胚胎移植 (IVF- ET)治疗不孕症 6 1例 6 4个周期的治疗结局。方法 接受治疗患者年龄 2 5岁至 41岁 ,共进行 6 4个周期的常规 IVF- ET治疗 ,于取卵后 48~ 5 0 h进行胚胎移植。结果 本组治疗周期 6 4个 ,取消周期 4.6 % ,取卵周期 6 1个 ,平均周期取卵数 (9.6 1± 6 .6 3)个 ,受精率 71.32 % ,卵裂率 90 .78% ,每周期胚胎移植数 (3.86± 1.0 9)个 ,临床妊娠率为 2 8.0 7%。结论  IVF- ET治疗输卵管梗阻性及原因不明不孕症在临床已获成功 ,提高妊娠率有待改善各种有关条件  相似文献   

8.
将21只健康恒河猴随机分成两组,分别用Polio病毒Ⅱ型MEF_1株小剂量(2×10~(7.5)TCD_(50))及大剂量(20×10~(7.5)TCD_(50))经腓肠肌感染。治疗组猴在感染后24小时静注10万~30万效价/公斤体重的抗Polio Ⅱ型病毒McAb(小鼠腹水)。感染前及感染后21天内定期采猴血2~3毫升,用原代猴肾细胞分离病毒及测定血清抗体效价,并作临床观察。感染后21天剖杀,进行组织病理学检查。初步实验结果:①小剂量病毒组中有2/6对照猴发生临床麻痹,治疗猴无一麻痹,大剂  相似文献   

9.
1991年在对本中心264只1岁半左右的幼年猕猴常规检疫中,发现37只感染了阔腹猴虱。本文报告对这批幼猴感染虱的调查情况,及以速灭杀了治疗的效果。材料与方法 1.动物 264只自繁幼年猕猴,1岁半左右;分群饲养在28个笼中,每笼7~14只,已达1年。 2.方法在常规检疫中逐只对幼猴进行体外寄生虫检查。发现有虱感染者,观察记录虱分布部位、虱及卵寄生密度。采集猴虱成虫及卵,并送贵阳医学院金大雄教授鉴定。以1%速灭杀丁浸浴已感染幼猴(除面部外)。7d后,检查药浴效果。结果 1.猴虱感染率 264只幼猴分养于28个猴笼中。其中3个笼的幼猴(37只)全部感染虱,而其它25笼幼猴则无1只感染。感染率  相似文献   

10.
我们于1985年引进食蟹猴疟原虫B株,观察其生物学特性,建成食蟹猴疟原虫B株—斯氏按蚊—恒河猴猴疟模型。B株原虫对斯氏按蚊感染性强,斯氏按蚊叮咬输血感染后7~11天的猴,其卵囊阳性率可达80~100%;第8天卵囊成熟,第10天子孢子进腺率平均52.7%。猴感染后第7天肝切片中红外期原虫不分叶,无  相似文献   

11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

12.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

13.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

19.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

20.
FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…  相似文献   

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