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1.
HPLC法同时测定复方利血平氨苯蝶啶片中3组分的含量   总被引:2,自引:0,他引:2  
目的建立测定复方利血平氨苯蝶啶片中氢氯噻嗪、硫酸双肼屈嗪和氨苯蝶啶含量的方法。方法采用高效液相色谱法,以氰基柱(4.6 mm×250 mm,10μm)为色谱柱,以0.01 mol/L庚烷磺酸钠溶液(用体积分数20%磷酸调节pH值至2.7)-乙腈(体积比85∶15)为流动相,流速1.0 mL/min,检测波长为316 nm。结果氢氯噻嗪、硫酸双肼屈嗪和氨苯蝶啶分别在60~180μg/mL的浓度范围内有良好的线性关系(r=0.999 9);氢氯噻嗪、硫酸双肼屈嗪和氨苯蝶啶平均加样回收率分别为100.2%、100.1%和100.3%,RSD值分别为0.36%、0.85%和0.83%(n=9)。结论本方法简便、快速、准确度高,可用于复方利血平氨苯蝶啶片的质量控制。  相似文献   

2.
HPLC法测定复方四嗪利血平片中氢氯噻嗪的含量   总被引:1,自引:1,他引:0  
目的:建立高效液相法测定复方四嗪利血平片中氢氯噻嗪含量的方法。方法:采用Diamonsil-C18(6.0mm×150mm,5μm)色谱柱;流动相:甲醇-水-磷酸盐缓冲液(30:66:4)(0.05mol/L磷酸二氢钾溶液,用磷酸调节pH至3.0);检测波长:272nm;流速:1.0ml/min。结果:氢氯噻嗪在0.0896-0.0896μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为98.92%,RSD=0.60%(n=6)。结论:本方法简便、准确、重现性好,可用于复方四嗪利血平片中氢氯噻嗪的质量控制。  相似文献   

3.
王光辉 《中国医药导刊》2008,10(9):1437-1438
目的:建立盐酸肼屈嗪中有机溶剂残留量的测定方法。方法:采用顶空进样毛细管气相色谱法,程序升温方式,DM-1毛细管柱,以氮气为载气,用FID检测器测定盐酸肼曲嗪中甲醇、乙醇、正己烷、正庚烷的残留量。结果:盐酸肼曲嗪中有乙醇检出。甲醇、乙醇、止己烷和正庚烷分别在1.19-59.35,1.99-99.26,0.12-5.78和1.98-99A8μg/mL的浓度范围内呈良好的线性关系;平均回收率分别为100.1%,99.78%,99.94%和99.87%。结论:本法灵敏、准确,可用于盐酸肼曲嗪中有机溶剂残留量的测定。  相似文献   

4.
反相高效液相色谱法测定沙棘油中维生素E的含量   总被引:9,自引:0,他引:9  
目的:采用反相高效液相色谱法测定沙棘油中维生素E的含量。方法:C18-ODS柱为色谱柱、甲醇-水(98:2)为流动相,流速为1.5ml/min,检测波长:280nm。结果:线性范围0.9-6.0μg(r=0.9998),平均回收率为99.17%,RSD为1.48%(n=5),结论:本法操作简便,准确可靠。  相似文献   

5.
浅析北京降压0号   总被引:6,自引:0,他引:6  
北京降压0号,具有降压和轻度镇静作用,适用治疗轻、中度高血压。其组成为:利血平0.1mg,硫酸双肼屈嗪12.5mg,氢氯噻嗪12.5mg,氨苯喋啶12.5mg,氯氮3mg。本药配方原理从三方面着手,即基础降压;协同降压;中枢神经调整以改善症状。1基础降压硫酸双肼屈嗪和利血平是基础降压药,抑制细小动脉收缩而使血压下降。其中硫酸双肼屈嗪通过松弛动脉平滑肌,使周围血管舒张,血压下降。而利血平能使交感神经节后纤维末梢贮存的传导介质去甲肾上腺素减少乃至耗竭,使发自中枢的兴奋传导受阻,不能到达效应器,失去收缩血管,兴奋心脏的作用。这两种红色物合用,降压…  相似文献   

6.
目的:建立培美曲塞二钠中有机溶剂残留量的气相色谱分析方法。方法:选用OV-101毛细管色谱柱为分离柱,FID检测器,外标法进行定量,并对分离条件进行了研究。结果:二氯甲烷、四氢呋喃、N,N-二甲基甲酰胺的线性范围分别为5.1~50.6μg/ml(r=0.9997)、8.1-81.2μg/ml(r=0.9996)、7.5-75.2μg,/ml(r=0.9997);平均回收率分别为99.74%、101.94%、100.58%;RSD分别为1.40%、2.07%、2.56%(n=6);检测限分别为0.4、0.4、1.7μg/ml。结论:该方法快速、灵敏、准确,可作为培美曲塞二钠中有机溶剂残留量的测定方法。  相似文献   

7.
高效液相法测定复方樟脑乳膏中葡萄糖酸氯己定含量   总被引:1,自引:0,他引:1  
目的:采用高效液相法测定复方樟脑乳膏中葡萄糖酸氯己定的含量。方法:以Kromasil--C18(250.0mm×4.6mm,5μm)色谱柱,流动相为甲醇-水-三乙胺(44:56:1)(用磷酸调节pH值至3.5±0.1),流速为1.0ml/min,检测波长258am。结果:线性范围为0.08-0.72μg,r=0.9999,平均回收率为99.92%,RSD=0.04%。结论:该方法便捷、灵敏、准确、重复性好,可以控制该药的质量。  相似文献   

8.
目的:建立一种同步测定车前子超微速溶饮片中京尼平苷酸与毛蕊花糖苷含量的高效液相色谱方法。方法:色谱柱为HypersilODS2C18柱(4.6mm×250mm,5p,m),流速:1.0mL/min,检测波长:254nm,柱温:35℃。结果:京尼平苷酸与毛蕊花糖苷的线性范围分别为59.88μg/mL~159.68μg/mL(r=0.9999)和91.26μg/mL~243.36μg/mL(r=0.9999);平均加样回收率分别为100.5%(RSD:1.1%,n=6)和99.7%(RSD=1.3%,n=6)。结论:本方法操作简便,测定结果准确,重复性好,可作为该产品质控的方法。  相似文献   

9.
目的:建立硫酸氢氯吡格雷的含量测定方法。方法:采用HPLC法,色谱柱Sino Chrom ODS-BP(4.6 mm×200 mm,5μm),流动相:1%三乙胺(pH=4.0)-乙腈(25∶75),检测波长:225 nm,流速:1.0 ml/min。结果:硫酸氢氯吡格雷进样量在0.48~0.72μg范围内呈良好的线性关系(r=0.999 9)。结论:本法简便、准确,重复性好,可用于该产品的质量控制。  相似文献   

10.
目的建立检测降压保健食品中非法添加化学成分氢氯噻嗪的定性、定量检测方法。方法采用薄层色谱法、高效液相色谱-二极管阵列检测法、LC/MS/MS联用技术进行定性鉴别,并采用高效液相色谱法测定其中氢氯噻嗪的含量。结果在1批市售保健品中检出氢氯噻嗪,氢氯噻嗪高效液相检测法在1.013~101.3μg/mL范围内线性关系良好(r=1.000 0),平均回收率为99.27%,RSD为1.41%。结论该方法快速、准确、灵敏、可靠,可用于有效监测保健食品中非法添加的氢氯噻嗪。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

20.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

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