常年性鼻炎患者血清和鼻分泌物总IgE测定及其临床意义

应用ＥＬＩＳＡ法对２０例常年性变应性鼻炎、２４例可疑变应性鼻炎、１９例常年性非变应性鼻炎患者的血清及鼻分泌物总ＩｇＥ含量进行检测，并与１０例健康者对照。结果常年性变应性鼻炎患者血清总ＩｇＥ显著高于对照组（Ｐ＜０．０５），可疑变应性鼻炎组和常年性非变应性鼻炎组血清总ＩｇＥ与对照组无显著性差异（Ｐ＞０．０５）。各组鼻分泌物总ＩｇＥ与对照组无显著性差异（Ｐ＞０．０５）。同时讨论了影响因素。     

变应性鼻炎筛前神经射频治疗前后IgE变化观察

目的　了解变应性鼻炎治疗前后血清总 Ｉｇ Ｅ、特异性 Ｉｇ Ｅ水平变化情况。方法　对８８ 例应用鼻内窥镜筛前神经射频治疗的变应性鼻炎患者进行治疗前后血清总 Ｉｇ Ｅ、特异性 Ｉｇ Ｅ 检测对比。结果　本组病人治疗前后血清总 Ｉｇ Ｅ、特异性Ｉｇ Ｅ均有显著性差异（ Ｐ＜ ００１）。结论　破坏鼻内筛前神经既可明显缓解变应性鼻炎的症状体征,也可使血清总 Ｉｇ Ｅ、特异性 Ｉｇ Ｅ水平降低。     

变应性鼻炎特异性免疫对细胞因子的影响

目的 探讨特异性免疫治疗前后变应性鼻炎患者血清中白细胞介素（ＩＬ－６，ＩＬ－８，ＩＬ－１２）的变化及意义。方法 用ＥＬＩＳＡ法检测健康体检者１９例，变应性鼻炎患者３１例血清中ＩＬ－６，ＩＬ－８，ＩＬ－１２水平。结果 变应性鼻炎患者特异性免疫治疗前与正常对照组比较，ＩＬ－６差异有显著性（Ｐ＜０ ０５）；ＩＬ－８，ＩＬ－１２差异有非常显著性（Ｐ＜０．０００１）；特异性免疫治疗前后相比，ＩＬ－８差异有非常显著性（Ｐ＜０．０００１），ＩＬ－６差异有显著性（Ｐ＜０．０５），ＩＬ－１２差异则无显著性（Ｐ＞０．０５）。特异性免疫治疗后与正常对照组比较，无显著性差异（Ｐ＞０．０５）。特异性免疫治疗前ＩＬ－６，ＩＬ－８与ＴＩｇＥ呈正相关，ＩＬ－１２与ＴｌｇＥ呈负相关。结论 特异性免疫治疗使变应性鼻炎者细胞固子ＩＬ－６，ＩＬ－８，ＩＬ－１２的分泌趋于正常，证实其为治疗变应性鼻炎较为有效的方法之一。     

类风湿关节炎患者的类风湿因子分类和IL—8

目的 探讨类风湿因子（ＲＦ）分类和白细胞介素－８（ＩＬ－８）在类风湿关节炎（ＲＡ）发病机制中的作用。方法 运用ＥＬＩＳＡ法检测１８例活动期ＲＡ及２５例缓解期ＲＡ患者血清中ＩｇＧ－ＲＦ、ＩｇＭ－ＲＦ和ＩＬ－８水平。结果 ①活动期ＲＡ组血清ＩｇＧ－ＲＦ、ＩｇＭ－ＲＦ与正常对照组有显著差异（Ｐ〈０．０５）,而ＩｇＡ－ＲＦ无统计学意义。②活动期ＲＡ组血清ＩｇＧ－ＲＦ、ＩｇＭ－ＲＦ与正常对照组有显著差异（Ｐ     

儿童变应性鼻炎与细胞因子的相关性研究

目的：探讨儿童变应性鼻炎与细胞因子及免疫球蛋白的关系。方法：采用ＥＬＩＳＡ双抗体夹心法及单向免疫扩散试验,对４０例变应性鼻炎患儿进行了血清白细胞介素１（ＩＬ１）、白细胞介素２（ＩＬ２）及免疫球蛋白的测定,同时设健康同龄儿３０例为对照组。结果：变应性鼻炎组ＩＬ１、ＩｇＥ明显增高,ＩＬ２降低,与对照组比较有非常显著差异（Ｐ＜０．０１）,ＩｇＧ、ＩｇＡ、ＩｇＭ无显著性差异（Ｐ＞０．０５）。结论：检测ＩＬ１、ＩＬ２、ＩｇＥ能对变应性鼻炎的诊断、治疗提供客观指标。     

儿童与成年人变应性鼻炎尘螨皮试与血清检测结果分析

目的分析儿童与成人变应性鼻炎对尘螨变应原皮肤试验与血清特异性IgE的检测结果。方法临床诊断变应性鼻炎成人86例,儿童42例。患者均接受尘螨变应性尘螨变应原皮肤试验及血清特异性IgE的检测。结果儿童组尘螨变应原皮肤试验与血清特异性IgE强阳性率（38．10％）高于成人组（20．93％）,弱阳性率（16．66％）低于成人组（29．07％）。结论变应性鼻炎患者通过变应原皮肤试验与血清特异性IgE,可以做到早期诊断、早期干预、早期治疗,特别是儿童对螨过敏患者较成人更适合早期接受免疫治疗。     

应用生物素—亲和素系统检测类风湿因子

目的 研究准确定量检测ＩｇＭ，ＩｇＧ，ＩｇＡ型类风湿因子（ＲＦ）的方法。方法 应用生物素－亲和素结合ＥＬＩＳＡ法（ＡＢＣ－ＥＬＩＳＡ）对５７例类风湿关节炎患者及１００例正常人血清进行检测，并与胶乳凝集试验（ＬＡＴ）及常规ＥＬＩＳＡ法测定的ＲＦ作对比。结果 ５７例病人ＩｇＭＲＦ阳性率在ＡＢＣ，ＥＬＩＳＡ法及ＬＡＴ法分别为８９．５％，８０．７％和６３．２％（Ｐ〈０．０１）。在ＬＡＴ法ＩｇＭＲＦ阴性的１     

变应性鼻炎特异性IgE抗体的测定

本文采用ＥＬＩＳＡ法对常年性变应性鼻炎，屋尘抗原皮肤试验示阳性反应的３６例患者，血清中屋尘特异性ＩｇＥ抗体了测定。３６例中特异ＩｇＥ抗体阳性２３例占６４％，皮肤试验反应强度与特异ＩｇＥ抗体之间无相关。ＥＬＩＳＡ法特异性高，特异ＩｇＥ抗体阳性时便可确定变应原，临床上有很大应用价值。     

哮喘患儿血清ECP,IgE,sIL—2R和IL—5水平及其临床意义的研究

为探讨哮喘患儿各期血清ＥＣＰ,ＩＬ－５,ｓＩＬ－２Ｒ和ＩｇＥ水平及其相关性和临床意义,用３７℃凝血１ｈ的血清,以法马西亚ＣＡＰ系统检测哮喘各期和对照组ＥＣＰ,以双抗体夹心ＥＬＩＳＡ法测定ＩＬ－５,ｓＩＬ－２Ｒ和ＩｇＥ。结果表明ＥＣＰ,ＩｇＥ和ｓＩＬ－２Ｒ均为发作期和稳定期高于缓解期和对照组,发作期不同程度高于稳定期,ＩＬ－５各期阳性率为Ａ５１．４３％,ＡＰ５６．０％,Ｓ３０．７７％,Ｎ１０．０％,     

肾综合征出血热患者血清ALT水平及其与特异性抗体和循环免疫复合物的关系分析

该文分析了肾综合征出血热患者（ ＨＦＲＳ） 血清ＡＬＴ 水平与临床分型及病期的关系,与血清特异性ＩｇＡ,ＩｇＥ,Ｉｇ Ｇ,Ｉｇ Ｍ 抗体、血清抗原特异性Ｉｇ Ｍ ,ＩｇＧ,ＩｇＡ,ＩｇＥ 循环免疫复合物（ ＣＩＣ） 的关系。发现ＡＬＴ 升高率以休克少尿期最高,次之为发热期,从多尿期始明显下降,恢复期最低;ＡＬＴ 水平（ ｘ ±ｓ） 以发热、休克少尿期明显升高而多尿、恢复期则明显下降,其水平以重型病例为最高,在发热期与轻、中型之间有明显差异（ Ｐ ＜ ０ ．０２５ ～０ ．０１） 。特异性Ｉｇ Ｍ ,ＩｇＧ,ＩｇＥ 抗体高滴度组ＡＬＴ 水平高于低滴度组,Ｉｇ Ａ 抗体则相反。检测特异性ＣＩＣ 的结果大致相同。提示ＨＦＲＳ 患者ＡＬＴ 升高主要在病程早期,且与特异性抗体及其ＣＩＣ 水平、病情严重程度密切相关     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…