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1.
    
目的利用PCR-DGGE方法分析应用变性梯度凝胶电泳技术,分析比较未经治疗和根管治疗失败的慢性根尖周炎患牙根管内的菌群结构。方法收集临床诊断为慢性根尖周炎的病例19例,包括未治疗患牙9例和根管治疗失败患牙10例。利用变性梯度凝胶电泳技术比较分析不同临床疾病状态患牙根管内细菌菌群结构的不同。结果每个样本中均可检测到细菌菌群的存在。未经治疗和根管治疗失败的慢性根尖周炎DGGE条带数差异有统计学意义(P0.05)。聚类分析以及PCA分析结果发现治疗后和未经治疗的根尖周病患牙感染根管内的微生物菌落结构相互之间是有着明显区别的。结论未经治疗与根管治疗失败的慢性根尖周炎根管内细菌菌丛存在明显差异;不同种属细菌的联合作用是导致根管治疗失败的主要原因。  相似文献   

2.
赵军 《吉林医学》2009,30(17):1939-1940
目的:探讨导致根管治疗失败的相关因素,为临床采取相应对策提供依据。方法:按照纳入标准选择根管治疗失败病例125例,分析根管治疗失败患牙146颗。记录主诉、临床表现和根尖周X线检查。分析根管治疗失败与相关影响因素的相关性。结果:125例146颗患牙,欠填84颗,恰填27颗,超填35颗。结论:根管治疗失败的主要原因是根管内的再次感染,主要是技术原因,患牙根尖周病变程度、根管解剖结构、牙体充填或修复的质量也可对根管治疗效果产生影响。  相似文献   

3.
目的:探讨临床根管治疗失败病例的常见原因.方法:回顾两年来疗效观察期内173例根管治疗失败病例,记录就诊原因、临床表现和X线检查. 总结分析治疗失败和根尖病变、充填程度的关系.结果:173例病例 179颗患牙,治疗失败病例发生根尖周炎的总共137颗,其中因不完善充填或不完善修复牙冠致冠渗漏49颗(27.37%),术前有窦道36颗(20.11%),根尖欠填33颗(18.44%), 牙周牙髓联合病变12颗(6.70%),根尖超填7颗(3.91%);发生牙折42颗(23.46%).结论:根管治疗失败的常见原因是治疗技术缺陷如不完善充填或修复牙冠、欠填、牙周牙髓联合病、超填、牙折造成根管内的继发感染,并与患者年龄、牙位及患牙的根管解剖情况等因素有关.  相似文献   

4.
张武  张莉  郭王敏 《医学综述》2009,15(17):2712-2713
目的分析基层医院根管治疗失败的原因,以进一步提高根管治疗成功率。方法回顾161例(174颗牙)根管治疗失败病例资料,根据症状、体征和辅助检查分析治疗失败与充填程度的关系。结果161例(174颗牙)失败病例发生原因为:欠充106例(116颗牙),超充21例(22颗牙),遗漏根管22例(24颗牙),冠折、冠部充填物松动或脱落等12例(12颗牙);根管治疗失败最多的牙位为磨牙74例(80颗),其次为前磨牙52例(56颗)和前牙35例(38颗)。结论根管治疗失败的主要原因是技术操作欠妥造成根管内再次感染。认真仔细地操作,有助于避免根管治疗失败。  相似文献   

5.
目的:分析显微超声技术去除根管内阻塞物的临床疗效。方法:对常规方法不能取出的38个根管内异物的病例,使用根管显微镜进行根管再处理,统计患牙的治疗成功率,并于术后6个月复查。结果:38颗患牙根管内的29支折断器械被完全取出,成功率76.3%。未发生根管侧穿或牙根纵裂。经复查无1颗患牙出现治疗失败。结论:显微超声技术的使用,为处理根管内折断器械提供了更有效的手段。  相似文献   

6.
薛雯  安涓  郭晓玫 《中外医疗》2013,32(17):57-58
目的探讨根管内器械折断后,患牙应用旁路通过法行根管治疗后的临床疗效。方法选择30颗根管内器械折断不能完全取出的患牙为实验组,应用从折断器械周围建立通路到达根管工作长度的方法进行根管预备和充填,完成根管治疗;对照组30颗牙常规根管治疗,随访观察2年,比较两组根管治疗后的疗效。结果实验组30颗患牙2年后回访牙数26颗,回访率86.67%,成功22颗,根管治疗成功率84.6%,对照组30颗患牙2年后回访牙数25颗,回访率83.33%,成功23颗,根管治疗成功率92.0%,两组2年后的根管治疗成功率差异无统计学意义(χ2=0.147,P>0.05)。结论根管内折断器械不能取出时,可以从折断器械周围建立旁路,经旁路完成根管治疗。  相似文献   

7.
薛雯  安涓  郭晓玫 《中外医疗》2013,32(16):51-52
目的探讨根管内器械折断后,患牙应用旁路通过法行根管治疗后的临床疗效。方法选择30颗根管内器械折断不能完全取出的患牙为实验组,应用从折断器械周围建立通路到达根管工作长度的方法进行根管预备和充填,完成根管治疗;对照组30颗牙常规根管治疗,随访观察2年,比较两组根管治疗后的疗效。结果实验组30颗患牙2年后回访牙数26颗,回访率86.67%,成功22颗,根管治疗成功率84.6%,对照组30颗患牙2年后回访牙数25颗,回访率83.33%,成功23颗,根管治疗成功率92.0%,两组2年后的根管治疗成功率差异无统计学意义(χ2=0.147,P>0.05)。结论根管内折断器械不能取出时,可以从折断器械周围建立旁路,经旁路完成根管治疗。  相似文献   

8.
慢性根尖周炎患牙根尖外表面的细菌生物膜   总被引:2,自引:0,他引:2  
目的:对慢性根尖周炎患牙根尖外表面的生物膜进行细菌学研究和形态学观察,了解生物膜中细菌的种类、组成及其超微结构.方法:选择临床诊断为慢性根尖周炎且不能保留的患牙10例,包括未经治疗及根管治疗失败的患牙各5例,同时将5例因正畸减数拔除的健康前磨牙作为对照组.所有样本的牙冠部无菌消毒后进行根管内取样、接种、厌氧培养后检测根管内的细菌种类.息牙拔除即刻3%(体积分数)戊二醛固定,取距根尖5 mm的牙根经梯度脱水、临界点干燥、喷金处理后进行扫描电镜观察.结果:10例慢性根尖周炎患牙拔除前根管内细菌培养均为阳性,其中微小消化链球菌和具核梭杆菌检出率较高.在5例未治疗的慢性根尖周炎患牙中仅有1例根尖外表面存在生物膜结构,主要由球菌组成;而在5例根管治疗失败的根尖周炎患牙的根尖外表面均可观察到浓密的细菌膜状结构,由球菌、杆菌及/或丝状菌组成,并且在超填的牙胶尖表面还观察到由球菌和丝状菌组成的典型的"玉米棒芯"样结构.对照组中则未有细菌存在.结论:慢性根尖周炎患牙的根尖外表面存在细菌生物膜结构,该生物膜的存在与临床上难治性慢性根尖周炎密切相关.  相似文献   

9.
目的从根管治疗失败患牙的感染根管内分离、鉴定粪肠球菌;建立粪肠球菌根管内感染体外模型,观察根管内粪肠球菌生物膜的形成。方法收集感染根管样本,培养鉴定粪肠球菌,测定生长曲线;收集离体前牙24颗,截冠保留牙根,完成根管预备,对照组置入无菌水培养,观察组进行粪肠球菌培养,鉴定并以扫描电镜观察。结果感染根管样本可分离到粪肠球菌;观察组8个根管内可见基质包裹细菌形成栅栏样结构粘附在根管壁,密集于根管下1/2段。结论感染根管内可分离到粪肠球菌,该菌在预备后牙根管下1/2段内壁形成生物膜。  相似文献   

10.
甘波 《大家健康》2013,(3):167-168
目的分析根管治疗失败的原因,以求从中吸取教训,进一步提高根管治疗治疗水平。方法选择根管治疗失败105例105颗患牙,根据临床检查及诊断对其原治疗失败原因加以分析。结果 105颗根管治疗失败的患牙中46颗为欠充;35颗为遗漏根管;18颗为超充;6颗为其他因素。结论以上各因素是根管治疗失败的主要原因。提高技术操作水平,严格掌握根管治疗的适应证,可提高根管治疗的成功率。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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