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1.
目的 探讨红毛五加多糖-Ⅰ的抑癌机制。方法 采用DNA电泳和流式细胞术检测红毛五加多糖-Ⅰ对胃癌细胞的影响。结果 该多糖作用36小时后,电泳可见胃癌细胞DNA的梯状图谱,流式图上出现凋亡峰。结论 表明红毛五加多糖-Ⅰ可能通过诱导胃癌细胞凋亡而起到抑癌作用。  相似文献   

2.
红毛五加多糖-Ⅰ诱导体外人胃癌细胞凋亡的研究   总被引:1,自引:0,他引:1  
目的 探讨红毛五加多糖 - 的抑癌机制。方法 采用 DNA电泳和流式细胞术检测红毛五加多糖 - 对胃癌细胞的影响。结果 该多糖作用 36小时后 ,电泳可见胃癌细胞 DNA的梯状图谱 ,流式图上出现凋亡峰。结论 表明红毛五加多糖 - 可能通过诱导胃癌细胞凋亡而起到抑癌作用  相似文献   

3.
红毛五加多糖对体外人胃癌细胞的抑制作用   总被引:4,自引:0,他引:4  
目的 研究红毛五加多糖对人胃癌细胞、人胚腱细胞及正常人外周血淋巴细胞的选择性抑制作用特点。方法 采用细胞形态学方法和MTT法。结果 该多糖选择性地抑制了胃癌细胞的活性和功能状态,而对人胚腱细胞影响甚微,对正常人外周血淋巴细胞无影响。结论 多糖选择性地抑制了人胃癌细胞,用于肿瘤的防治可能较为安全。  相似文献   

4.
目的 :研究反义c myc寡核苷酸抑制大鼠气道平滑肌增殖与细胞凋亡的关系 .方法 :大鼠气道平滑肌细胞原代培养 ,4~ 12代用于实验 .利用Lipofectin将正义、反义及错配c myc寡核苷酸导入大鼠气道平滑肌细胞 ,姬姆萨染色查找凋亡小体 ,流式细胞技术观察有无凋亡峰出现 ,琼脂糖电泳观察凋亡细胞特有的梯状图谱 ,免疫组织化学染色检测凋亡相关基因bax和bcl 2 .结果 :反义c myc寡核苷酸处理大鼠气道平滑肌细胞 2 4h后 ,细胞生长明显受到抑制 (0 .12± 0 .0 3vs 0 .19± 0 .0 5 ,P <0 .0 1) ,但无凋亡小体发现 ,流式细胞技术没有检测到凋亡峰 ,细胞总DNA琼脂糖电泳没有观察到典型的梯状凋亡图谱 ,免疫组织化学染色显示凋亡相关基因产物Bax及Bcl 2在各组表达无明显差异 .结论 :反义c myc寡核苷酸抑制大鼠气道平滑肌增殖与细胞凋亡无关  相似文献   

5.
目的 :探讨肝细胞生长因子对肿瘤坏死因子α(TNF -α)诱导的肝细胞凋亡的保护作用以及可能的机制。方法 :选择第 3代人类肝细胞系 (L0 2细胞 ) ,分成三组 :Ⅰ组 ,正常L0 2细胞 ;Ⅱ组 ,10 0 0 0u/mlTNF -α损伤L0 2细胞 2 4h ;Ⅲ组 ,重组肝细胞生长因子 (r -hHGF ,5ng/ml)与L0 2细胞共同孵育 30min后加入 10 0 0 0u/mlTNF -α ,2 4h后处理细胞。观察“Ladder”条带、细胞DNA断裂百分率和用Westernblot印迹分析法检测HSP70和IκB -α的表达。结果 :Ⅱ组DNA电泳出现典型的“梯状”条带 ,而Ⅲ组则未见明显的“梯状”条带。DNA断裂百分率Ⅱ组比Ⅰ组高 (分别为 17.12± 4 .0 2和 5 .0 5± 1.6 7,P <0 .0 1) ,Ⅲ组比Ⅱ组低 (分别为 8.77± 2 .0 1和 17.12± 4 .0 2 ,P <0 .0 1)。重组肝细胞生长因子预处理后再用TNF -α损伤的L0 2细胞 ,1h即可检测到HSP 70的表达 ,且持续到 4 8h。肝细胞生长因子还抑制了TNF -α引起的L0 2细胞IκB -α量的减少。结论 :重组肝细胞生长因子可减轻TNF -α诱导的L0 2细胞凋亡 ,它通过诱导L0 2细胞HSP 70的表达和抑制IκB-α的降解达到减轻L0 2细胞的凋亡。  相似文献   

6.
应用相差显微镜、电子显微镜、流式细胞术和DNA电泳等方法观察MGC-803胃癌细胞系凋亡的发生。结果显示30μmoL/L bcl-2反义寡核苷酸、2μg/ml顺铂、20μg/ml卡铂、4μg/ml阿霉素、4μg/ml丝裂霉素处理胃癌细胞,24h后观察到凋亡小体出现、染色质浓缩、凋亡峰及DNA梯状条带等凋亡特征性改变。  相似文献   

7.
红毛五加多糖含量测定及急性毒性实验研究   总被引:4,自引:0,他引:4  
红毛五加为五加科五加属植物,主要分布于四川、甘肃、宁夏等地.<神农本草经>记载红毛五加有"祛风湿,通关节,强筋骨"等作用.我们对甘肃产红毛五加水提取物、醇提物及其总甙、多糖成分的抗炎、解热镇痛、镇静、保肝、对免疫功能的影响等多方面药理作用进行了研究[1~3],现对其多糖的含量及急性毒性进行了测定.  相似文献   

8.
蜂毒素对胃癌细胞系SGC-7901细胞增殖抑制作用及机制   总被引:2,自引:1,他引:1  
目的 研究蜂毒素影响人胃癌细胞SGC-7901增殖及凋亡的机制.方法 使用不同浓度(1、2、4、8、16、32μg/ml)蜂毒素处理SGC-7901细胞不同时间(12、24、36、48、72 h)后,采用MTT法测定肿瘤细胞生长抑制率;透射电镜观察细胞超微结构的改变;琼脂糖凝胶电泳观察凋亡细胞特征性DNA条带;流式细胞仪法检测细胞凋亡和细胞周期;免疫组化观察Bcl-2基因和Bax基因表达情况;RT-PCR检测Bcl-2、Bax、p53 mRNA的表达.结果 蜂毒素对胃癌SGC-7901细胞的生长抑制作用随着其作用时间的延长和浓度的增加而明显增强,作用48 h后,其IC50为2.43 μg/ml;透射电镜现察显示蜂毒素组细胞呈凋亡表现,核固缩、碎裂,染色质浓缩,边集;蜂毒素(4、8μg/ml)组培养48 h后DNA琼脂糖凝胶电泳可见典型的梯状条带,而对照组细胞DNA未见梯状条带;流式细胞DNA直方图上出现典型的亚二倍体"凋亡峰",2、4、8μg/ml浓度蜂毒素作用人胃癌细胞后的细胞凋亡率分别为(5.69±0.74)%、(10.58±1.32)%、(29.57±1.58)%.Bax、p53基因mRNA和蛋白表达降低、Bcl-2 mRNA和蛋白表达升高,并随蜂毒素浓度增加而加强.结论 蜂毒素能诱导人胃SGC-7901细胞凋亡和抑制增殖,且有一定的量效关系,其作用机制可能与调控Bcl-2、Bax、p53基因表达有关.  相似文献   

9.
目的:研究舒林酸对胃癌细胞株BGC一823增殖、凋亡的作用,探讨其作用机制. 方法:体外培养的胃癌细胞株BGC-823加入不同浓度的舒林酸作用不同时间后,用倒置显微镜观察细胞形态学变化,四甲基噻唑氮蓝(MTT)法检测细胞生长衣抑制率,流式细胞术(flow cytometry,FCM)检测细胞周期;透射电镜观察细胞超微结构变化及凋亡形态变化,DNA电泳检测细胞凋亡DNA片段. 结果:舒林酸可以改变胃癌细胞株BGC-823的形态,抑制其生长,影响其细胞周期分布,透射电镜观察到细胞凋亡的形态,DNA电泳检测出典型的细胞凋亡的特征性梯状条带. 结论:舒林酸有抑制胃癌细胞株BGC-823增殖,诱导其凋亡的作用,并且可能与舒林酸影响其细胞周期分布、抑制了环氧合酶-2(COX-2)表达有关.  相似文献   

10.
红毛五加多糖-Ⅰ单糖组成的气相色谱分析   总被引:1,自引:0,他引:1  
目的研究红毛五加多糖-Ⅰ(AHP—Ⅰ)的单糖组成,及它们的摩尔比。方法采用糖腈乙酰酯衍生物气相色谱法,分析多糖中的单糖组成。采用相对校正因子法测定多糖中单糖的摩尔比。结果AHP—Ⅰ含有阿拉伯糖、岩藻糖、甘露糖、葡萄糖、半乳糖,其摩尔比为:0.32:0.45:0.20:1.00:0.67。结论红毛五加多糖AttP-Ⅰ由多种单糖构成,糖腈乙酸酯衍生化适合于红毛五加多糖的分析。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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