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1.
板蓝根抗内毒素作用研究 总被引:16,自引:1,他引:15
刘云海 《中国药科大学学报》1995,26(5):297-299
1%板蓝根氯仿提取物溶液(药液)有抗大肠杆菌(E.Coli)O111B4内毒素(内毒素)作用。家兔热原法实验结果显示:实验组(药液2ml/kg+内毒素40EU/kg)、阳性对照组(内毒素40EU/kg)、阴性对照组(药液2ml/kg)平均升温分别为0.08,0.77,0.10℃。用电子显微镜观察内毒素结构形态,发现经药液作用后的内毒素由链状变为片状。鲎试验法证实,该药液稀释32倍仍有抗内毒素作用。 相似文献
2.
免疫吸附清除肿瘤坏死因子对内毒素休克兔肾脏的作用 总被引:3,自引:0,他引:3
应用抗肿瘤坏死因子(TNF)单克隆抗体免疫吸附方法,特异性清除循环TNF,观察对内毒素休克肾脏的作用。方法以致死剂量内毒素(8×109cfu/kg)制成内毒素休克动物模型,1小时后开始体外循环免疫吸附,监测平均动脉压(MAP)变化,循环TNF及尿素氮(BUN)、肌酐(Cr)水平。6小时后活杀动物,留取肾脏行组织病理检查。结果(1)免疫吸附组血液灌流后30分钟MAP开始回升(11.6±1.4kPa),明显高于对照组(8.8±2.3kPa,P<0.01);(2)免疫吸附组2小时的TNF活性(44±10U/ml)较对照组(1448±226U/ml)明显降低(P<0.05);血清BUN、Cr水平较对照组降低(P<0.05);(3)免疫吸附组肾小球充血,白细胞浸润及肾小管坏死程度和线粒体的损害均较对照组明显减轻。结论抗TNF单克隆抗体特异性免疫吸附方法,能有效清除血循环TNF。早期降低TNF水平,有效地缓解内毒素休克时的低血压状态,减轻肾脏病理损害,改善肾功能 相似文献
3.
石楠藤提取物对急性出血坏死性胰腺炎大鼠治疗作用的实验研究 总被引:2,自引:0,他引:2
作者报道中药石楠藤提取物对急性出血坏死性胰腺炎(AHNP)大鼠治疗作用的实验研究。将100只SD大鼠随机分为三组:①假手术组(SO组);②AHNP组(AP组),该组动物在AHNP诱发后,不做特殊治疗;③石楠藤提取物治疗组(SN组)。该组动物在AHNP诱发后10分钟经腹腔内注射石楠藤提取物(1%石楠藤提取物5ml/kg体重)。结果表明:SN组血清淀粉酶值明显下降(SN组16130±5030U/L6h、19290±5380U/L12hvsAP组23780±3960U/L、28470±4150U/L,P<0.01)。血中PAF含量明显降低(SN组1.3±0.30ng/ml1h、2.0±0.34ng/ml6h、2.1±0.32ng/ml12hvsAP组2.2±0.25ng/ml、6.1±0.42ng/ml、10.1±0.53ng/ml,p<0.01)。血浆内毒素含量明显下降(SN组69.9±7.2EU/L、6h、91.1±8.4EU/L、12hvsAP组98.4±6.9EU/L、151.7±9.1EU/L,P<0.01)。石楠藤提取物治疗组术后平均存活时间明显长于AP组(SN组:43.5h,AP组11.5h,p<0 相似文献
4.
恶性梗阻性黄疸内、外引流术后血浆内毒素含量变化与死亡率的关系 总被引:1,自引:0,他引:1
目的:了解内、外引流在治疗恶性阻梗性黄疸的疗效。方法:对150例恶性梗阻性黄疸患者分为内引流组110例,外引流组10例,比较两组在引流术后70天内血浆内毒素的变化和死亡率关系。结果:内引流组在术后血浆内毒素与术前相比有下降趋势,内毒素值从(0.60±0.22)Eu/ml下降到(0.21±0.03)Eu/ml,病死率1.8%(2/110)。而外引流组结果则相反,术后血浆内毒素与术前相比呈上升趋势,内毒素值从(0.61± 0.20)Eu/ml上升到(1.19±0.08)Eu/ml,病死率70%(28/40)。结论:内毒素血症在恶性阻梗性黄疸引流术后早期是导致死亡的主要原因,对恶性梗阻性黄疸患者应尽可能施行胆管内引流术。 相似文献
5.
目的:研究ZG对烧伤后肠道免疫功能的调节。方法:40%体表面积(TBSA)Ⅲ度烫伤大鼠灌喂复方紫归(ZG)3g/kg,2/d,共3d(ZG组),灌喂0.9%生理盐水8ml/kg,2/d,共3d(对照组)。结果:ZG组肠道内容物IgA含量明显高于对照组(P<0.05);ZG组肠固有层CD3+T淋巴细胞活性明显增强(P<0.05);ZG组血浆LPS、MDA明显低于对照组(P<0.05)。结论:复方紫归对烫伤大鼠肠道免疫功能有一定的保护作用,可减轻肠道缺血再灌注损伤和肠源性内毒素血症。 相似文献
6.
寻找内毒素和培养的内皮细胞直接结合的证据及对胞内连接蛋白α-Catenin的影响,观察内毒素对内皮细胞的损伤特性。方法:(1)荧光标记的内毒素(2μm/ml)和培养的内皮细胞在无血清下共同孵育后用荧光分光光度计进行检测;(2)内毒素(2μg/ml)刺激无血清培养的内皮细胞后用ELISA测定内皮细胞胞内连接蛋白α-Catenin表达的变化。结果:内皮细胞和内毒素孵育10、20、40和60分后荧光强度分别为对照组的1.87、3.97、3.41(P<0.01)和2.02(P<0.05)倍;胞内连接蛋白的表达各时相点和对照组比均明显降低( P<0. 05和<0. 01)。结论:内毒素在无血清情况下可以和内皮细胞结合,同时可以影响胞内连接蛋白α-Catenin的表达状态,并不依赖是否有血清的存在。 相似文献
7.
黄显荣 《广西中医学院学报》2000,17(4):63-64
「目的」探讨清热解毒药的抗内毒素作用。为临床寻找高效低毒的抗内毒素药物。「方法」选10种清热解毒分别经鉴别、提取、浓缩。配成含原生药400mg/ml、20mg/ml,10mg/ml,5mg/ml的药液,分别与等体积1Eu/ml细菌内毒素作用2小时,然后再分别为特异性鲎试剂进行试验,「结果」具有抗内毒素作用的药物及浓度分别为:金银花、玄参、苦参、黄柏(5mg/ml);黄芩、黄连、七叶一枝花(10mg/ml);连,鱼腥草,生地(20mg/ml)。「结论」金银花、玄参、苦参、黄柏等有较强抗内毒素活性。 相似文献
8.
家蚕细胞基因工程人α-干扰素注射液(IHIFN-α)具有抑制肿瘤生长的作用。对于离体培养的HL60和KB细胞,IHIFN-α有直接抑制肿瘤细胞生长的作用,且呈剂量依赖性关系。在体研究中,IHIFN-α2.0×106IU/kg对S180有较明显的抑制趋向(P<0.01),抑瘤率达21.0%;而对于EAC,IHIFN-α4.0×105IU/kg及2.0×106IU/kg两个剂量都无明显延长生存时间的作用;IHIFN-α与5-Fu10mg/kg合用对S180小鼠抑瘤率以及EAC小鼠存活天数并无增效作用;但是5-Fu10mg/kg与2.0×106IU/kgIHIFN-α合用却能明显减小EAC小鼠第9天的腹围(P<0.05,与5-Fu比较)。 相似文献
9.
甲状旁腺全切加前臂移植术对红细胞生成素治疗肾性贫血的作用 总被引:2,自引:2,他引:0
目的:研究10例患者行“甲状旁腺全切(PTX)加前臂移植术”对红细胞生成素治疗肾性贫血的有益作用。方法:观察10例继发性甲状旁腺功能亢进(SHPT)患者手术前、后3~56个月平均血细胞比容(HCT)和重组人类红细胞生成素(rHuEPO)的使用剂量,同时检测血清全段甲状旁腺素(iPTH)并与术前3个月的HCT、rHuEPO的使用剂量作相关分析。结果:8例患者PTX1个月后,血HCT开始上升,术后3个月,10例患者的平均HCT由术前(21.4±1.8)%升至(29.1±1.9)%,rHuEPO的平均剂量由术前每周(100.0±8.3)U/kg(60~150U/kg)降至(70.4±6.1)U/kg(60~85U/kg)。随访2~5年,疗效稳定可靠。PTX前、后rHuEPO的剂量及HCT的各自改变差异均有显著性(%P%<0.05)。iPTH也由术前(1484.75±72.40)pg/ml降为(50.13±9.30)pg/ml。HCT与iPTH呈负相关。结论:PTX能明显改善慢性肾功能衰竭(CRF)患者合并SHPT的贫血,提高rHuEPO的疗效。 相似文献
10.
灰树花多糖以2000,100O,500mg/kg/d的剂量给NIH小鼠灌胄7天,然后腹腔注射1280HAU/ml的NDV(新城鸡瘟病毒)0.5ml。6h后放血,分离血清,以L929细胞、VSV病毒检测鼠干扰素。结果发现,灰树花多糖能够促进NDV诱生干扰素。各实验组、NDV对照组和生理盐水对照组的干扰素活性分别为44.5,45.0,29.0,12.0和2.0U/ml,据此认为,灰树花多糖抗病毒感染的作用可能由干扰素系统介导,但也可能有其他机理参与。 相似文献
11.
Tao Ye * Zhi-quan Liu Jian-jun Mu Xi-han Fu Jun Yang Bao-lin Gao and Xiao-hong Zhang Department of Cardiology First Hospital of Xi'an Jiaotong University Xi'an Hanzhong Cardiovascular Institute Hanzhong 《中国医学科学杂志(英文版)》2004,19(4):248-251
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure. 相似文献
12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD. 相似文献
13.
Jiageng Zhu Jun Li Ruipeng Ji Jianghao Su Mingshun Shen Zhigang Cao 《南京医科大学学报(自然科学版)》2007,21(2):125-128
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment. 相似文献
14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center. 相似文献
15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine. 相似文献
16.
Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine 下载免费PDF全文
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes. 相似文献
17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P. 相似文献
18.
Journal of Nanjing Medical University 《南京医科大学学报(英文版)》2007,21(1):I0001-I0002,F0003
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China. 相似文献
19.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险. 相似文献
20.
Jingming Zhu Ningning Hui Xiuhong Jiang Huaxing Zhang Chengzhang Fu 《南京医科大学学报(自然科学版)》2007,21(6):386-389
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients. 相似文献