Minimally modified low-density lipoprotein induces monocyte chemotactic protein-1 expression in vivo and a novel model for monocyte adhesion to arterial intima

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Human monocyte-derived dendritic cells expressing both chemotactic cytokines IL-8, MCP-1, RANTES and their receptors, and their selective migration to these chemokines

Ｄｅｎｄｒｉｔｉｃｃｅｌｌｓ(ＤＣｓ)ａｒｅｔｈｅｍｏｓｔｐｏｔｅｎｔａｎｔｉｇｅｎｐｒｅｓｅｎｔｉｎｇｃｅｌｌｓａｎｄｓｐｅｃｉａｌｉｚｅｉｎｓｔｉｍｕｌａｔｉｎｇｒｅｓｔｉｎｇＴｃｅｌｌｓａｎｄｉｎｉｔｉａｔｉｎｇＴｃｅｌｌｄｅｐｅｎｄｅｎｔｉｍｍｕｎｅｒｅｓｐｏｎｓｅｓ1ＤＣｐｒｅｃｕｒｓｏｒｓｏｒｉｇｉｎａｔｅｉｎｔｈｅｂｏｎｅｍａｒｒｏｗａｎｄｓｕｂｓｅｑｕｅｎｔｌｙｍｉｇｒａｔｅｉｎｔｏｐｅｒｉｐｈｅｒａｌｔｉｓｓｕｅｓａｎｄｐｒｉｍａｒｙｌｙｍｐｈｏｉｄｏｒｇａｎｓｗｈｅｒｅｔｈｅｙ…     

Discernment of the sensitized inner ear by peripheral immunocompetent lymphocytes

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Insulin-like growth factor binding protein 1 and human embryonic development during 6-10 gestational weeks

Theinsulin likegrowthfactor (IGF)systemplaysanimportantroleinfetaldevelopment ThefollowingelementscomprisetheIGFsystemunderconsideration :IGFs ,includingIGF I,IGF II;Insulin likegrowthfactorreceptors(IGF Rs) :IGF R1andIGF R2 ;Insulin likegrowth factorbindingproteins (IGFBPs) ,involving 6homologousbindingproteins ,IGFBP 1,a 2 5 3kDproteinwith 3- 4phosphorylatedforms ,isthemostimportantcomponent DuringpregnancyIGFBP 1isfound ,notonlyinliverandmammarygland ,butisalsosynthesize…     

Immunogenecity and tumor-inhibiting effects of HSP-antigen peptide complex in melanoma B16 cells in mice

目的:黑色素瘤B16细胞热休克蛋白-抗原肽复合物(HACs)及其粗提物(HAC-CEs)的制备,以及它们的免疫原性和抑瘤效应的研究.方法:应用Sephacryl S-200凝胶过滤制备HAC-CEs,应用亲和层析纯化HACs,并测其免疫功能和抑瘤效应.结果:应用凝胶过滤制备的HAC-CE3、HAC-CE4、HAC-CE5和应用亲和层析纯化的HAC60,HAC75和HAC97均不同程度地降低肿瘤发生率、延迟肿瘤发生时间和减少移植黑色素瘤C57BL/6J小鼠死亡率;同时,伴有小鼠脾细胞IFN-γ和IL-2分泌活性及CTL杀伤率的增加.结论:分子量为60000～97000的HACs具有免疫原性和抑瘤效应,本研究为制备肿瘤疫苗提供重要的实验依据.     

Immunogenecity and tumor-inhibiting effects of HSP-antigen peptide complex in melanoma B16 cells in mice

目的:黑色素瘤B16细胞热休克蛋白-抗原肽复合物(HACs)及其粗提物(HAC-CEs)的制备,以及它们的免疫原性和抑瘤效应的研究.方法:应用Sephacryl S-200凝胶过滤制备HAC-CEs,应用亲和层析纯化HACs,并测其免疫功能和抑瘤效应.结果:应用凝胶过滤制备的HAC-CE3、HAC-CE4、HAC-CE5和应用亲和层析纯化的HAC60,HAC75和HAC97均不同程度地降低肿瘤发生率、延迟肿瘤发生时间和减少移植黑色素瘤C57BL/6J小鼠死亡率;同时,伴有小鼠脾细胞IFN-γ和IL-2分泌活性及CTL杀伤率的增加.结论:分子量为60000～97000的HACs具有免疫原性和抑瘤效应,本研究为制备肿瘤疫苗提供重要的实验依据.     

Expression of glycoprotein non-metastatic melanoma protein B in cutaneous malignant and benign lesions: a tissue microarray study

Background  Glycoprotein non-metastatic melanoma protein B (GPNMB) plays an important role in the pathogenesis of inflammatory and malignant diseases. We investigated the expression of GPNMB in benign and malignant skin diseases.

Methods  Tissue microarray was performed in the skin tissues of 102 cases including malignant melanoma (MM), squamous cell carcinoma (SCC), basal cell carcinoma (BCC), and benign dermatosis. The expression of GPNMB in the tissues was detected by immunohistochemistry. Twenty cases of normal skin and adjacent neoplastic normal skin tissues were selected as controls.

Results  GPNMB was positively stained in skin malignancies (38/50, 76%), which was significantly higher than that in the control and the benign skin tissues (P=0.001 and <0.001 respectively). GPNMB was positively stained in MM (13/15, 87%) and SCC (16/20, 80%) (P <0.001). Significant higher expression of GPNMB was observed in patients aged ≥65 years than those less than 65 years (n=11 and n=9 respectively, P=0.027). No significant difference of the expression rates was observed between normal control and BCC; however, stronger intensity was detected in the latter. Negative or weak expression was observed in the controls.

Conclusion  Over-expression of GPNMB correlated strongly and might play an important role in the pathogenesis of MM and SCC.

     

Interleukin-1β,Tumor Necrosis Factor-α and Lipopolysaccharide Induce Expression of Monocyte Chemoattractant Protein-1 in Calf Aortic Smooth Muscle Cells

The focal influx and accumulation of lipoprotein at arterial lesion--prone sites and the recruitment of blood monocytes to those sites are two early key events in atherogenesis. After entering thesubendothelial space of the arterial wall, monocytes undergo activation--differentiation to becomemacrophages which subsequently internallze oxidatively modified lipoproteins through scavenger receptors and become foam cells. Therefore, tostudy the mechanism of the recruitment of bloodmonocytes to the…     

Effect of oxymatrine on specific cytotoxic T lymphocyte surface programmed death receptor-1 expression in patients with chronic hepatitis B

Background  Oxymatrine has certain antiviral effects in the treatment of chronic hepatitis B (CHB), but its exact mechanism is unclear. The objective of the present study was to explore oxymatrine’s antiviral mechanism by studying its effect on the hepatitis B virus (HBV) specific cytotoxic T lymphocyte (CTL) surface programmed death receptor-1 (PD-1) expression in CHB patients.

Methods  Sixty-five CHB patients who had HBV DNA³10⁴ copies/ml, positive HBeAg, positive human leukocyte antigen (HLA)-A2, alanine aminotransferase (ALT) >2´upper limit of normal value (ULN) were randomly divided into two groups: treatment group (n=33), treated with an intravenous infusion of 600 mg oxymatrine in glucose solution once a day for a month, then with a 200 mg oxymatrine oral capsule three times a day, and a 200 mg silibin meglumine tablet three times a day; control group (n=32) patients were treated only with silibin meglumine tablet, method and dosage were the same as those of treatment group. Three months later, peripheral blood HBV-specific CTL surface PD-1 expression, HBV-specific CTL level, HBV DNA, HBeAg, and results of liver function tests were analyzed and compared.

Results  Three months post-treatment, in the treatment group, peripheral blood HBV-specific CTL surface PD-1 expression ((19.42±15.94)%) decreased significantly compared to the pretreatment level ((31.30±24.06)%; P <0.05), and decreased significantly compared to that of control group three months after treatment ((29.45±21.62)%; P <0.05). HBV-specific CTL level ((0.42±0.07)%) significantly increased compared with the pretreatment ((0.29±0.15)%; P <0.01), and the control group posttreatment level was (0.31±0.15)% (P <0.05). HBV DNA level in 11 cases became negative (HBV DNA<500 copies/ml, 33.33%), which was higher than that of the control group after treatment (two cases, 6.25%; χ²=7.45, P <0.01), HBeAg of nine cases turned negative (27.27%), which was higher than that of the control group after treatment (one case, 3.13%; χ²=7.27, P <0.01).

Conclusion  Oxymatrine could downregulate peripheral blood HBV-specific CTL surface PD-1 expression in CHB patients, increase HBV-specific CTL level, which may be one of the possible mechanisms by which oxymatrine clears or inhibits HBV in CHB patients.

     

Correlation between T lymphocyte subsets in peripheral blood lymphocytes and 2-year all-cause mortality in an apparently healthy elderly Chinese cohort

Background  Few data have been acquired on the predictive value of age-related T-lymphocyte subsets among older individuals. The present study has determined the distribution of T-cell phenotypes and their correlation to 2-year mortality in a cohort of Chinese male seniors.

Methods  A total of 101 asymptomatic elderly individuals with laboratory homeostasis were enrolled at baseline. Three age subgroups were categorized as young (65–74 years old), middle (75–84 years old ), and old (≥85 years) for age-related comparison. T-cell subsets in peripheral blood were measured by multi-colored flow cytometry.

Results  At baseline, there was a mild negative correlation by age for total lymphocytes and CD3⁺ T-cells. The frequency of CD28 and CD95 demonstrated a “curved” rather than linear tendency by age. At 2-year follow-up, little change of T-cell distribution was found among those who remained alive (as survivors) comparing the data at baseline to the 2-year time point. Immune risk phenotypes were distinctly demonstrated between survivors and non-survivors.

Conclusions  Since few studies have studied on the distribution of T-lymphocyte subsets in an elderly Chinese population, our results have not only provided reference values of T-subsets for aged Chinese men, but confirmed the immune risk phenotypes among elderly Chinese. The inappropriate age-dependent trajectory of CD28^–/CD8⁺ and CD95^–/CD8⁺ by age, which suggested 85 might be an inflexion point of age during T-cell ageing, warrants further exploration of the underlying mechanisms of T-cell ageing.

     

MCP-1在肾小球肾炎中的表达及其意义

目的：探讨肾小球肾炎患者肾组织单核细胞趋化蛋白－１（ＭＣＰ－１）的表达及其与肾间质中浸润的单核细胞（ＭＣ）数、肾小管病理损伤、尿蛋白量之间的关系。方法：用免疫组化技术检测５例正常及３１例原发性肾炎肾组织中ＭＣＰ－１、ＣＤ６８的表达。结果：正常肾组织有极少量ＭＣＰ－１表达及ＭＣ浸润;膜性肾小球肾炎（ＭＮ）组肾小球内未见ＭＣＰ－１、ＣＤ６８表达;系膜增生性肾小球肾炎（ＭｓＰＧＮ）、膜性增生性肾小球肾炎     

Surveillance testing in patients with primary malignant melanoma

There are, at this time, no fully published prospective randomized studies of follow-up strategies for patients with primary malignant melanoma. However, four recent retrospective studies indicated that greater than 90% of melanoma recurrences were detected by history and/or physical examination, and that detection of recurrent melanoma by blood tests, chest X-ray, or CT scan rarely led to long-term survival. Based on the results of the above studies, recommendations for follow-up as developed by the National Institute of Health Consensus Panel on Early Melanoma can be considered. These include: 1) history and physical examination (including total body skin examination) at regular intervals; 2) the consideration of initial screening for first degree family members of patients with melanoma; and 3) on-going education for patients to recognize the signs of local and regional recurrences.     

E-cadherin、MCP-1和MIP-1β在B16黑色素瘤荷瘤鼠中的表达及意义

目的 检测皮肤B16黑色素瘤小鼠体内E-cadherin、MCP-1、MIP-1 β的表达,探讨其与树突状细胞在体内迁移过程中的调控的关系.方法 取C57纯系小鼠30只,分为12 d肿瘤组,25 d肿瘤组及正常对照组,每组10只.肿瘤组将B16黑色素瘤细胞人工植入C57小鼠背部皮下,制荷瘤鼠模型,采用免疫组织化学SABC法检测肿瘤病变中心区及交界区皮肤中E-cadherin、MCP-1、MIP-1β表达.结果 肿瘤接种后第12天的MCP-1和MIP-1 β在淋巴结和肿瘤中心区和交界区表达的面密度和数密度明显增高,分别与对照组和肿瘤第25天组比较差异有显著性(P<0.01).肿瘤组的E-cadherin在淋巴结和肿瘤组织中表达降低,第25天组尤其明显;第25天组与第12天组E-cadherin表达分布面密度和数密度比较差异有显著性(P<0.01),第12天组和对照组比较差异有显著性(P<0.01).结论 在肿瘤状态下,E-cadherin、MCP-1和MIP-1 β有促进树突状细胞向淋巴组织和肿瘤局部迁移和聚集的作用.E-cadherin、MCP-1、MIP-1 β的异常表达在皮肤黑色素瘤的恶性进展过程中起重要作用.     

MCP-1在IgA肾病患者肾组织中的表达

目的:检测IgA肾病患者肾活检组织中的单核细胞趋化蛋白-1(MCP-1)的表达,并对其表达水平与病变类型进行相关性分析。方法:88例IgA患者肾活检组织,根据?plusmn;砀谋浞治嵛⒉”湫汀⑾的ぴ錾汀⒕衷钣不秃兔致不?组,免疫组织化学SP法检测MCP-1和CD68在肾组织中的表达,采用图像分析法对其表达进行半定量分析,同时收集患者的一般临床资料。结果:肾小球中MCP-1表达在4组中无统计学差异,而在肾小管间质中MCP-1的表达分别为轻微病变组1.43±0.60、系膜增生组5.98±0.92、 局灶硬化型组10.60±0.76、弥漫硬化型组11.65±0.39,局灶硬化型组、弥漫硬化型组明显高于轻微病变组、系膜增生组,差异有统计学意义(P<0.01);肾小球内几乎未见CD68＋细胞;而肾小管间质中CD68的表达在4组中分别为:0.75±0.71,5.87±0.96,10.42±0.61,11.40±0.49,差异有统计学意义(P<0.01)。MCP-1在肾小管间质内的表达与CD68在肾小管间质中的表达呈正相关(r=0.688,P<0.01);MCP-1在肾小管间质内的表达与24 h尿蛋白量呈明显正相关(r=0.531,P<0.01)。结论:MCP-1对IgA肾病患者肾组织中单核细胞的浸润起重要作用,MCP-1的表达水平与肾脏损害的程度及临床预后相关。     

警惕老年人以疼痛为首发症状的转移性骨肿瘤

本文报告5例老年患者,首发症状均表现为全身多处疼痛,进一步检查证实为转移性骨肿瘤,其中4例原发病灶未能明确。提示在老年人疼痛疾病诊疗过程中医生应该具备扎实的医学基础理论知识,进行详细、全面的病史询问和体检,采取合理的辅助检查方法及诊治流程,拓展临床思维,积极寻找原发病,以免漏诊、误诊。     

AOPP患者血清MCP-1的表达及意义

目的探讨在急性有机磷农药中毒患者中血清单核细胞趋化蛋白-1的表达及临床意义。方法选取2010年5月-2011年8月来我院人院治疗的急性有机磷农药中毒患者153例,以患者的中毒程度为依据进行分组,其中轻度组、中度组及重度组分别有患者37、61、55例。血清单核细胞趋化蛋白-1的含量采用双抗体夹心酶联免疫吸附法进行检测。结果与对照组比较,急性有机磷农药中毒的各组患者中的MCP-1具有明显较高的含量,CHE较低;与轻度中毒组比较,重度组及中度组的MCP-1均具有明显较高的含量,CHE较低;与中度组比较,重度组明显具有较高的MCP-1含量,CHE较低;且以上差异均具有统计学意义（P〈0．05）。MCP-1含量与血清CHE呈显著的负相关（P〈0．05）。与生存组比较,死亡组患者的MCP-1含量差异有统计学意义（P〈0．05）。结论在急性有机磷农药中毒患者中对其MCP-1含量进行检测,能够使疾病的病情及其预后得到反映,急性有机磷农药中毒患者的病情越重,中毒程度越深,其MCP-1含量越高,并且与胆碱酯酶呈显著的负相关。     

单核细胞趋化蛋白-1对67例全身炎症反应综合征患者的诊断价值

目的 评估单核细胞趋化蛋白-1(MCP-1)在全身炎症反应综合征(SIRS)中的诊断价值。 方法 前瞻性研究。收集天津医科大学第二医院重症监护病房(ICU)、泌尿外科及胃肠外科于2020年8月至2021年4月收住院的67例经诊断符合SIRS的患者作为病例组,对照组为同期体检人员44例。采用酶联免疫吸附法(ELISA)检测病例组与对照组血清中的MCP-1浓度并比较其差异。将病例组按是否存在多器官功能障碍(MODS)分为MODS组(n=26)和非MODS组(n=41),比较血清MCP-1浓度,绘制受试者工作曲线,评价血清MCP-1对SIRS伴MODS的诊断效能。根据患者临床表现及检测结果,将患者分为脓毒症组(n=15)和非脓毒症组(n=52),比较两组间血清MCP-1浓度。 结果 SIRS患者血清MCP-1中位水平为180.2(90.9~407.4)pg/mL,对照组血清中位水平为53.4(40.2~83.7)pg/mL(P<0.001)。SIRS患者中MODS组血清MCP-1中位水平为426.2(150.1~733.6)pg/mL,非MODS组血清MCP-1中位水平为135.0(55.6~236.1)pg/mL(P<0.001),以血清MCP-1为变量对SIRS伴MODS诊断效能的ROC曲线下面积为0.74,最大约登指数对应的血清MCP-1临界值为176.0 pg/mL,敏感度为76.9%,特异性为65.9%。脓毒症组与非脓毒症组血清MCP-1比较差异无统计学意义(P=0.832)。 结论 SIRS患者血清MCP-1检测对MODS具有辅助诊断价值,但不能作为SIRS进展为脓毒症的预测指标。     

原发性女性生殖器恶性黑色素瘤16例分析

目的：探讨原发性女性生殖器恶性黑色素瘤的临床病理特点及合理的治疗方法。方法：对16例原发性女性生殖器恶性黑色素瘤的临床资料进行回顾性分析。结果：发生于外阴9例,阴道5例,子宫颈2例。全部病例均行手术加化疗综合治疗,行区域淋巴结清扫11例,4例有转移,转移率36.3％。存活＜2年8例,≥2年8例,其中≥5年仅2例。结论：原发性女性生殖器恶性黑色素瘤预后差,行根治手术辅化疗和免疫治疗,可望提高生存期。     

冠心病患者血浆同型半胱氨酸与单核细胞趋化蛋白-水平的变化

目的观察冠心病患者血浆同型半胱氨酸和MCP-1浓度的变化,并分析Hcy对血浆MCP-1表达的影响.方法所有入选的研究对象均经冠状动脉造影检查,其中冠心病组70例,对照组50例.应用高压液相色谱法测定血浆Hcy浓度,标准方法测定血浆丙二醛(MDA)浓度,ELISA法检测血浆MCP-1浓度.结果①冠心病组血浆Hcy、MDA及MCP-1浓度均显著高于对照组(P<0.05).②冠心病组患者的血浆同型半胱氨酸浓度与MCP-1浓度呈明显线性相关.结论高同型半胱氨酸血症可能通过提高MCP-1的表达、增加脂质过氧化损伤,而促进动脉粥样硬化的发病.     

应用MALDI-TOF-MS技术分析转移和非转移黑色素瘤患者外周血清蛋白的差异表达

目的：应用蛋白质组学技术分析转移性和非转移性黑色素瘤患者外周血清中差异表达的蛋白质,初步探讨其对转移性黑色素瘤患者的诊断及治疗指导意义。方法：共收集101例患者外周血清,包括25例转移性黑色素瘤患者及76例对照者（31例非转移性患者和45例健康者）。从中随机抽取73例（18例转移患者,20例非转移患者和35例健康者）用于建立区分模型,其余28例（7例转移患者,11例非转移患者和10例健康者）用来对区分模型进行盲法验证。运用基质辅助激光解离飞行时间质谱技术,对外周血清蛋白质进行检测,应用Ciphergen蛋白质芯片软件对蛋白质谱进行分析。结果：黑色素瘤转移患者外周血清与对照组外周血清蛋白质谱相比,质荷比为2 598.33、3 646.79、4 605.48、8 603.10、7 756.64 m/z和9 598.82 m/z,共6个蛋白质峰呈表达有明显差异（P均=0.000）,以此构建出转移性黑色素瘤的诊断模型,它的诊断敏感性为83.33%,特异性为74.55%。另外对该模型进行了盲法验证,其诊断转移性黑色素瘤的敏感性为71.43%,特异性为76.19%。结论：黑色素瘤转移患者外周血清蛋白质表达谱与对照组相比有明显变化,相对分子质量为2 598.33、3 646.79、4 605.48、8 603.10、7 756.64 m/z和9 598.82 m/z的蛋白质点可以作为黑色素瘤转移患者诊断的潜在生物学标志物。