空气中苯、甲苯和二甲苯的直接热解吸气相色谱测定法

本法特点是灵敏、快速、简便和不使用溶剂。用泵或注射器将含有待测物质的空气以3～5ml/s的速度通过Tenax GC吸附管。采好样后将Tenax GC管放入热解吸器中,在250℃下解吸,同时由氮气将释放以来的物质运送到色谱柱中,以氢水焰离子化检定器进行测     

潜艇空气污染物定性分析

我国对潜艇空气中的有害气体曾作过现场定量测试,但未见有系统的定性分析结果的报道。为了全面了解我国潜艇舱室空气中污染物的组成,为今后潜艇有害气体毒理学研究及艇用空气净化,装置设计提供依据,我们开展了潜艇空气污染物定性分析。取样和分析方法 1.取样和样品予处理用多孔聚合物作为空气取样吸附剂,操作简便,效果好,适于潜艇环境下使用。我们对使用的三种吸附剂(Chromosorb-103、GDX-101、Tenax GC)的活化温度、解吸温度、适用性、回收率进行摸索,对不同的污染物,应选用不同的吸附剂。取样管用长20cm,内径4mm的U形玻璃管制成,内装多孔聚     

吸唾管道两种清洁消毒方法对口腔诊室空气质量的影响

目的 口腔诊疗中含有大量细菌及病毒的气溶胶易造成周围环境及患者创口的感染,及时有效的吸唾是控制口腔诊室气溶胶污染的有效方法。本研究旨在探讨口腔门诊综合治疗台吸唾管道2种清洁消毒方法对空气质量的影响。 方法 2016年7月-2017年6月合肥市口腔医院采用2种方法清洁吸唾管道,2016年7-12月为对照组使用常规方法,2017年1-6月为实验组在对照组的基础上,第2步冲洗时增加1:200多酶清洗剂3 000 ml进行管道清洁。每月对诊室空气及吸唾器接口处进行采样,并观察吸唾管道的清洁度及异味情况。 结果 清洁消毒后,2组空气菌落数培养分别为(2.667±0.970) CFU/(皿·5 min)、(5.222±2.579) CFU/(皿·5 min),实验组低于对照组,差异有统计学意义(t=-3.934,P<0.05);吸唾器接口菌落数培养分别为(1.889±1.745) CFU/cm2、(4.667±3.125) CFU/cm2,实验组低于对照组,差异有统计学意义(t=-3.293,P<0.05);吸唾软管清洁度、异味程度结果比较,实验组优于对照组,差异有统计学意义(χ2=10.395,P<0.05)。 结论 改进后清洁消毒方法能有效降低诊室空气及吸唾器接口菌落数,同时有效增加吸唾管道清洁度并降低管道异味。改进后的清洁消毒方法简单易行,取材方便且效果可靠,值得在口腔门诊推广应用。      

用固相微萃取和气-质联用技术分析菘蓝叶中的挥发性成分

异硫腈酸盐是芥属蔬菜中典型的成分,具有化学防癌作用。由于菘蓝Isatis tinctoria L.叶中可能存在挥发性异硫腈酸盐,且对该植物中挥发性成分的研究鲜有报道,因此作者采用顶空固相微萃取(HS-SPME)和气-质(GC-MS)联用技术分析菘蓝叶中的挥发性成分。将3.5g样品溶于含5mL水的管形瓶中,平衡30min,在750r/min条件下搅拌萃取25min,萃取温度30℃。用于分析物热解吸的固定相纤维保持在260℃,共3min,观察多项参数对萃取最佳化的影响。在GC-MS分析中,采用带有Varian2000离子阱质谱仪的Varian3800气相色谱仪。使用2种不同的溶凝硅石毛细管柱:1)…     

稻秆热解固体产物的性能

稻秆热解的目的是为了脱除氧元素,增加能量密度,并将热解的固体产物作为生物质气流床气化原料,从而提高气化合成气的热值。稻秆在管式电阻炉中以5℃/min和15℃/min的升温速率进行慢速热解,热解温度为200~800℃。利用自动量热仪和元素分析仪对热解后固体产物进行热值和元素分析。结果表明:同一热解温度下,升温速率越快,半焦的热值越高;碳元素的含量随着热解温度的升高逐渐增加,而氢和氧两种元素的含量随着热解温度的升高逐渐降低。同时,推导出热值与半焦中各元素含量的关系式。     

HS－SPME/GC/MS法测定水中土臭素和2-甲基异崁醇的条件优化

目的:探索顶空固相微萃取-气相色谱/质谱法(HS－SPME/GC/MS)测定水中嗅味物质土臭素(GSM)和2-甲基异崁醇(2－MIB)的最佳实验条件?方法:应用L32(49)正交表安排顶空固相微萃取-气相色谱/质谱法测定水中嗅味物质时的萃取温度?萃取时间?搅拌子转速?盐浓度?解吸时间和解吸温度并进行实验,优化各项操作参数?结果:顶空固相微萃取-气相色谱/质谱法测定水中嗅味物质的优化后实验条件是:萃取温度和解吸温度分别为65℃和260℃,萃取时间和解吸时间分别是40 min和7 min,搅拌子转速是600 r/min,盐浓度为30%(W/V)?分别测定高低加标样,回收率为91%~107%,检出限(S/N=3)为0.04 ng/L,回收率为91%~104%,检出限(S/N=3)为0.13 ng/L?结论:通过正交实验设计优化实验条件,所建立的分析方法准确?简便?可靠,符合水中嗅味物质的测定要求?     

不同空气消毒方法在供应室无菌区中的应用研究

目的：探讨不同空气消毒方法对供应室无菌区空气消毒效果。方法：采用现场实验法应用紫外线或空气消毒洁净器分别对相同的供应室无菌区（A间和B间）进行空气消毒,比较消毒效果。结果：消毒前A间与B间空气采样细菌培养菌落数相似,差异无统计学意义（ P＞0．05）;消毒结束和消毒后30 min、60 min和120 min,B间空气采样细菌培养菌落数明显低于对照组,差异有统计学意义（ P＜0．05）。消毒结束时,空气消毒洁净器消毒可使空气中自然菌消亡率达到97．58％,而紫外线对空气中自然菌的消亡率为91．62％,差异有统计学意义（ P＜0．05）。结论：紫外线或空气消毒洁净器对供应室无菌区空气消毒均能达到国家要求,但是空气消毒洁净器效果更好,消毒后空气中细菌总数回升缓慢。     

犬冠状动脉支架植入术的模型建立

目的建立稳定的犬冠状动脉支架植入模型并探讨提高其成功率的方法.方法在DSA机器下行经颈动脉路径的冠状动脉支架植入术.结果 25只杂种犬中24例成功完成支架植入,1只因麻醉不当术中死亡,19只长期存活.24例中有3例钢丝在进入主动脉弓后指向降主动脉而需要以左冠Judkin's造影管引导至升主动脉再更换右冠指引导管(GC).24例中右冠脉Judkin's GC直接到位17例,另7例GC需要热塑型或需要在半到位状态下以导引钢丝先进冠脉进而引导到位.GC指向回旋支15例,指向前降支9例.平均透视时间前12例为(18±3) min,后12例为(11±5) min;造影剂平均用量前12例(90±35) mL,后12例(78±28) mL.支架植入前降支11例,回旋支13例,均为直接支架术.其中预装支架9例,自己安上球囊15例,均未发生支架脱落.结论经颈动脉犬冠脉支架植入模型成功率高,稳定性好,其技术关键在于GC的良好到位.     

犬冠状动脉支架植入术的模型建立

目的建立稳定的犬冠状动脉支架植入模型并探讨提高其成功率的方法.方法在DSA机器下行经颈动脉路径的冠状动脉支架植入术.结果 25只杂种犬中24例成功完成支架植入,1只因麻醉不当术中死亡,19只长期存活.24例中有3例钢丝在进入主动脉弓后指向降主动脉而需要以左冠Judkin's造影管引导至升主动脉再更换右冠指引导管(GC).24例中右冠脉Judkin's GC直接到位17例,另7例GC需要热塑型或需要在半到位状态下以导引钢丝先进冠脉进而引导到位.GC指向回旋支15例,指向前降支9例.平均透视时间前12例为(18±3) min,后12例为(11±5) min;造影剂平均用量前12例(90±35) mL,后12例(78±28) mL.支架植入前降支11例,回旋支13例,均为直接支架术.其中预装支架9例,自己安上球囊15例,均未发生支架脱落.结论经颈动脉犬冠脉支架植入模型成功率高,稳定性好,其技术关键在于GC的良好到位.     

气相色谱法同时测定广西莪术挥发油中莰烯、吉马酮、-榄香烯的含量

目的准确测量广西莪术挥发油中莰烯、吉马酮、-榄香烯的含量。方法采用气相色谱法(GC),Elite-WAX ETR(0.25 m×0.25mm×30m)柱;流速为2.0 mL/min,进样量为1 L,进样温度为250℃,分流比为20∶1;FID检测器温度为280℃,氢气流量为45.0 mL/min,空气流量为450 mL/min;程序升温:65℃保持恒温2 min,然后以5℃/min的速率上升到90℃后保持恒温3 min,然后以7℃/min的速率上升到105℃后保持恒温7 min,最后以6℃/min的速率上升到190℃后保持恒温20 min。结果三种物质的方法学考察均符合要求,准确测定了3个批次广西莪术挥发油样品中莰烯、吉马酮、-榄香烯的含量。结论 GC法测定广西莪术挥发油中莰烯、吉马酮、-榄香烯的含量简便、快捷。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.