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1.
In this study, total blood lymphocytes were prepared from patients with hemorrhagic fever with renal syndrome (HFRS) by a density gradient on Ficoll-Hypaque. T and B cells were then purified by passing the total lymphocytes over a nylon wool column. The purities of total lymphocytes, T cells and B cells were 97.8 +/- 2.3%, 91.6 +/- 4.5% and 74.2 +/- 12.1%, respectively. Also, after modification of cell fixation and smear drying, the number of cells were increased and the time needed for slide preparation was shortened. Detection of viral antigen by immunofluorescence assay using monoclonal antibodies to Hantavirus (HTNV) showed that the total lymphocytes. T cells and B cells were infected by HTNV during the early stages of HFRS and no specific fluorescence was seen in the cells from the late diuretic phase to convalescent phase. The results suggest that virus replication in blood lymphocytes may partly contribute in the early stages to the impairment of cell immune response and in vivo spread of HTNV to its target sites.
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2.
Background Cryptotanshinone (CT) was originally isolated from the dried roots of Salvia militorrhiza, an herb that is used extensively in Asian medicine and the extracts of this herb have been used in the treatment of several pathologies, including cardiovascular diseases, hematological abnormalities, hepatitis, and hyperlipidemia, but no studies had been carried on the treatment for rheumatic diseases with it. This study aimed to investigate the effects of cryptotanshinone on immune functions in rats with adjuvant arthritis (AA). Methods Complete Freund's adjuvant was used to induce AA in rats. Thymus and spleen was aseptically taken from normal rats and the AA rats. Then a thymus lymphoid cell suspension, splenic lymphoid cell suspension and peritoneal macrophage cell suspension were prepared. After adding CT (0.1 ug/ml, 1.0 ug/ml, 10 ug/ml, 100 ug/ml, 1000 ug/ml) into the suspension, T and B lymphocytes proliferation was determined by 3-(4,5-2 dimethylthiazal-2yl)2,5- diphenyltetrazoliumbromide (MTT) assay. And the activities of interleukin-1 (IL-1) and IL-2 were measured by the mouse lymphocytes proliferation assay. Results Thymic T and splenic B lymphocyte proliferation of the AA rat was significantly lower, and could be stored through using CT in vitro. CT (100ug/ml and 1000ug/ml) increased T or B lymphocytes proliferation in vitro (P 〈0.01). In AA rats, the levels of IL-1 released by abdominal PMφ significantly increased whereas the level of IL-2 released by T cells decreased in vitro. CT (1000 pg/ml) decreased the production of IL-1 and promoted production of IL-2 in vitro (P 〈0.05). Conclusions CT can ameliorate the abnormal immunological functions in AA rats.  相似文献   

3.
Background Epstein-Barr virus (EBV) is a herpesvirus commonly associated with several malignant diseases including nasopharyngeal carcinoma (NPC), which is a common cancer in Southeastern Asia. Previous studies showed that plasma levels of EBV-DNA might be a sensitive and reliable biomarker for the diagnosis, staging and evaluating of therapy for NPC. There are a few analyses of the levels of EBV-latent membrane protein 2 (LMP2)-specific cytotoxic T-lymphocytes (CTLs) in patients with NPC. This study was conducted to investigate the levels of EBV-LMP2-specific CTLs, EBV-DNA load and the level of CD4^+CD25^+T cells in such patients.
Methods From February 2006 to April 2006, 62 patients with NPC, 40 healthy virus carriers positive for EBV viral capsid antigen (EBV-IgA-VCA) and 40 controls were enrolled in the study. We used a highly sensitive ELISPOT assay, real-time polymerase chain reaction (PCR) and flow cytometry to measure the EBV-LMP2-specific CTL response, the EBV DNA load and the level of CD4^+CD25^+T cells, respectively.
Results The EBV-LMP2-specific CTL responses of the samples from the control, healthy virus carriers and patients with NPC were significantly different from the LMP2 epitopes, with the control and healthy virus carrier samples displaying a stronger response in three cases. There were significant differences in EBV DNA load in serum between NPC and the healthy groups; patients with NPC at stages Ⅲ or Ⅳ had significantly higher viral loads compared with those at stages Ⅰ or Ⅱ. A significantly higher percentage of CD4^+CD25^+ T lymphocytes were detected in the patients, compared with healthy virus carriers and healthy controls. Moreover, patients with advanced stages of NPC (Ⅲ and Ⅳ) had significantly higher percentages than the patients with early stages (Ⅰ and Ⅱ). Conclusions Patients with NPC are frequently unable to establish or maintain sufficient immunosurveillance to control proliferating B cells harboring EBV and to destroy the tumor cells that express immunodominant LMP2 proteins.
Controlling the activity of CD4^+CD25^+T cells and elevating CD8^+ cells specific for LMP2 epitopes could be an effective immunotherapy for patients with NPC.  相似文献   

4.
The function of CD4+CD25+ regulatory T lymphocytes (Treg) in patients with acute coronary syndrome (ACS) and the effects of atorvastatin were investigated. Forty-eight patients with ACS were randomly divided into two groups: group C receiving conventional therapy (n=24), and group C+A receiving conventional therapy+atorvastatin (10 mg/day, n=24). T lymphocytes from ACS patients (before and 2 weeks after the treatment) or 18 healthy subjects were separated and the flow cytometry was used to measure the percentage of Treg. The inhibitory ability of Treg on effector T cells was determined by mixed lymphocyte reaction (MLR). ELISA was used to measure the serum levels of cytokines (IL-10, TGF-β1 and IFN-γ) before and after treatment. The results showed that as compared with normal control group, Treg percentage was decreased significantly (P〈0.01), the inhibitory ability of Treg on the T lymphocytes proliferation was reduced (P〈0.01), IFN-γ levels were increased and IL-10 and TGF-β1 levels were lowered in ACS patients. After treatment with atorvastatin, Treg percentage and the inhibitory ability of Treg on T lymphocytes proliferation were significantly increased in ACS patients. Serum IFN-γ was decreased significantly, while IL-10 and TGF-β1 were elevated significantly as compared with the non-atorvastatin group. The number of Treg was positively correlated with serum TGF-β1, but negatively with serum IFN-γ and CRP. It was concluded that ACS was associated with decreased number and defected function of Treg, which may play an important role in initiating immune-inflammatory response in ACS. The inhibitory effects of atorvastatin on inflammation in ACS may be due to its beneficial effects on Treg and restoration of immune homeostasis.  相似文献   

5.
Background Chemoresistance is common among patients with esophageal squamous cell carcinoma (ESCC).We investigated the effect and mechanism of insulin on enhancing anticancer functions of cisplatin in human esophageal cancer cell line EC9706.Methods The viability of EC9706 cells exposed to cisplatin was assessed using MTT assay.The times T1,when the number of living cells reached a plateau and T2,when the number of living cells reached a new plateau after the addition of insulin were found.T1 and T2 plateau cells were stained by Annexin V-FITC/PI and monodansylcadaverin (MDC).Fluorescent microscopy was used to observe the expression of apoptosis and autophagy intuitively.Apoptotic ratio and fluorescent intensity were analysed by flow cytometry (FCM) quantitatively.Western blotting analysis was used to estimate the protein expression levels of AKT,mTOR,PI3K,PTEN,autophage related indicator LC3-Ⅱ and autophage related protein Beclin1 changes that occurred in the course of treatment.Results A larger number of typical autophagosomes were detected in EC9706 cells exposed to cisplatin.Insulin can increase the apoptosis induced by cisplatin.Apoptotic ratio of T1 plateau cells ((32.6±4.3)%) is significantly less than T2 plateau ((47.5±5.6)%).MDC fluorescent intensity at T1 plateau (104.9±13.2) was significantly higher than intensity at T2 plateau (82.6±10.3).After cotreatment with insulin,the expression level of LC3-Ⅱ,Beclin1 and PTEN in T2 plateau cells were significantly downregulated,but AKT,mTOR and PI3K expressions significantly upregulated compared with T1 plateau.Conclusions Insulin could enhance cisplatin-induced apoptosis in human esophageal squamous cell carcinoma EC9706 cells related to inhibition of autophagy.The activation of PI3K/Akt/mTOR signaling pathway induced by insulin resulted in the suppression of autophagy in EC9706 cells,which may be attributed to the anticancer effects of cisplatin.  相似文献   

6.
Objective To investigate the possibility of using melanoma antigen- 1 (MAGE- 1) peptide as a tumor vaccine to treat hepatocellular carcinoma (HCC). Methods The expressions of MAGE- 1 in 8 HCC cell lines and in liver cancer tissue from a patient were detected using RT- PCR. The type of human leucocyte antigen Ⅰ (HLAⅠ) of both 8 HCC cell lines and peripheral blood mononuclear cells of the patient was detected using a microcytotoxicity method to screen out target cell lines for the cytotoxicity assay. Peripheral blood mononuclear cells from the HCC patient pulsed with an MAGE- 1 peptide (NYKCRFPEI) were used as antigen presenting cells. Autogenous peripheral blood mononuclear cells were stimulated with antigen presenting cells every 7 days for 4 times to elicit cytotoxic T lymphocytes. The phenotype of effector cells was analyzed using flow cytometry. The cytotoxicity of effector cells was detected with a lactate dehydrogenase releasing assay. Results The expressions of both MAGE- 1 and HLA- A24 were detected in BEL7405 cell line which were used as the positive target cell line in the cytotoxicity assay. The expression of MAGE- 1 alone was detected in HLE, BEL7402, BEL7404, QGY7703 and SMMC7721 cell lines, and the expression of neither MAGE- 1 nor HLA- A24 was shown in QGY 7701 and HpG2 cell lines. The last 7 cell lines could be used as negative target cell lines in the cytotoxicity assay. Peripheral blood mononuclear cells expanded 32 folds during 28- day culture. The ratio of CD3[ + T cells in creased by 16% (from 54% to 70%), and the ratio of CD8[ + T cells increased by 20% (from 36% to 56%) during 28- day culture. When the ratio of effector cells to target cells was 10∶1,effector cells exhibited 62. 5% cytotoxicity against autogenous lymphoblasts pulsed with the peptide (NYKCRFPEI) of MAGE- 1 antigen, 40. 25% cytotoxicity against BEL7405 cells, compared with 17. 88% cytolysis observed against autogenous lymphoblasts, 19. 55% against HLE cells, and 1. 6% against QGY7701 cells. When the ratio of effector cells to target cells was 3. 3∶1, the cytotoxicity of effector cells against the peptide pulsed autogenous lymphoblasts was 53. 6%, which was much higher against autogenous lymphoblasts, HLE cells and QGY7701 cells at 15. 6%, 13% and 1%, respectively. Conclusion The results demonstrate that cytotoxic T lymphocytes with the ability to specifically lyse target cells expressing both MAGE- 1 and HLA- A24 could be successfully induced by the MAGE- 1 peptide NYKCRFPEI in vitro. This indicates that a good result might be anticipated if this peptide is used as a tumor vaccine to treat HLA- A24 HCC patients.  相似文献   

7.
The immune mechanism of Graves' diseases (GD) and the roles of regulator T cells were investigated. In 32 patients with GD (GD group) and 20 healthy volunteers (control group), flow cy-tometry was used to detect the proportion of CD4 CD25 cells, MACS to isolate CD4 CD25 cells, RT-PCR to assay the expression of FOXP3, and ELISA to test the level of IL-10, respectively. It was found that there was no significant change in the proportion of CD4 CD25 T cells between GD group and control group (P>0.05), while secretion of IL-10 and expression of FOXP3 in GD group were lower than control group (P<0.01 and P<0.05, respectively). In conclusion, though the proportion of regulatory T cells of peripheral blood lymphocytes in the patients with GD, the functions of them were significantly weakened, which might be a pathogenic factor in GD.  相似文献   

8.
Persistent hepatitis B virus (HBV) infection is the most important reason for chronic hepatitis B,hepatic cirrhosis, and hepatocellular carcinoma. T lymphocytes, including CD4^+ and CD8^+ T cells, are major composition of host cellular immunity. Furthermore, CD8^+ cells play a primary role in host immune reaction of anti-tumor and anti-infection. It has been confirmed that HBV infection leads to disorder of cellular immune function in patients, especially disorder in regulative function of T lymphocyte subgroups and cytokine. It has been paid more attention to the expression of HLA class I on hepatocytes infected by HBV and tumor cells, but less to the expression of HLA class I on peripheral blood lymphocytes in the past. In this study, we evaluated the expression of HLA class I and II on peripheral blood lymphocytes in patients with chronic hepatitis B, hepatic cirrhosis, and hepatocellular carcinoma and tried to provide new thought for the mechanism of disorder of celluar immunity.  相似文献   

9.
Background To investigate the expression of negative costimulatory molecule B7-H3 on CD3 T lymphocytes in colorectal cancer, and its relationship with clinic-pathological variables and patients’ prognosis. Methods The expression of B7-H3 was detected in 98 patients of colorectal cancer by using immunohistochemistry. Then the expression of B7-H3 was further conformed by flow cytometry on CD3 T lymphocytes isolated from the fresh cancer tissues of 12 colorectal cancer patients. Then analyzed the relationship between the expression of B7-H3 on CD3 T lymphocytes and patients’ clinic-pathological variables. Results The survival time of patients with more CD3 T cell infiltration are longer than those with less CD3 T cell infiltration(P<0.05), B7-H3 was highly expressed on the infiltrating CD3 T lymphocytes in colorectal cancer tissues. The expression of B7-H3 was found to be significantly related with the lymph node metastasis(P<0.05), but not with the patient's gender, age, tumor size, differentiation degree, depth of tumor invasion, Dukes' stage, distant metastasis and whether or not mucinous adenocarcinoma(P>0.05). Moreover, the survival time of patients with low expression of B7-H3 is longer than those of high B7-H3 expression patients, but the patients’ seven-year survival rate has no statistical difference between the high expression of B7-H3 and low expression of B7-H3(P>0.05). Conclusions The negative costimulatory molecule B7-H3 expresses on infiltrating CD3 T lymphocytes in colorectal cancer and plays an important role in lymph node metastasis and prognosis.  相似文献   

10.
Objective To detect the response of lymphocytes to radiation in untreated breast cancer patients with three different genetic assays. Methods Blood samples were collected from 25 untreated patients and 25 controls. Each blood sample was divided into two parts: one was irradiated by 3-Gy X-ray (irradiated sample), the other was not irradiated (non-irradiated sample). The radiosensitivity of lymphocytes was assessed by comet assay, cytokinesis-block micronucleus (CBMN) assay and 6-TG-resistant cells scored (TG) assay. Results The baseline values of micronucleated cell frequency (MCF) and micronucleus frequency (MNF) in the patients were significantly higher than those in the controls (P〈0.01), and 3-Gy X-ray induced genetic damage to lymphocytes in the patients increased significantly as compared with that in the controls as detected with the three genetic assays (P〈0.01). The proportion of radiosensitive cases in the patient group was 48% for the mean tail length (MTL), 40% for the mean tall moment (MTM), 40% for MCF, 44% for MNF, and 48% for mutation frequencies of the hprt gene (Mfs-hprt), respectively, whereas the proportion of radiosensitive cases in the control group was only 8% for all the parameters. Conclusion The difference in the lymphocyte radiosensitivity between the breast cancer patients and the controls is significant. Moreover, there are wide individual variations in lymphocyte radiosensitivity of patients with breast cancer. In some cases, the radiosensitivity of the same patient may be different as detected with the different assays. It is suggested that multiple assays should be used to assess the radiosensitivity of patients with breast cancer before therapy.  相似文献   

11.
目的:研究较大样本寻常型天疱疮(pemphigus vulgaris,PV)患者,血清抗桥粒芯蛋白(desmoglein,Dsg)1和抗Dsg3特异性抗体酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)指数长期的变化情况,观察其与病情变化的相关性,探讨其用于病情监测、预测疾病复发和指导治疗的可行性。方法:对20例确诊PV的患者进行长期随访,收集患者在各随访时间点的血清,记录每次随访时的病情并进行评分(autoimmune bullous skin disorderintensity score,ABSIS);检测患者血清的间接免疫荧光(indirect immunofluorescence,IIF)滴度、Dsg3 ELISA指数和Dsg1 ELISA指数;利用统计学方法和做图方法分析病情评分与Dsg3 ELISA指数、Dsg1 ELISA指数和IIF滴度之间的关系。结果:PV患者皮肤和口腔的病情评分,分别与Dsg3 ELISA指数、Dsg1 ELISA指数和IIF滴度均具有显著性关联(P<0.01),患者疾病活动期和临床缓解期两组间Dsg3 ELISA指数、Dsg1 ELISA指数和IIF滴度差异均有统计学意义(P<0.01)。各组患者相关性分析发现,Dsg3 ELISA指数、Dsg1 ELISA指数和IIF滴度几乎均与病情平行波动变化,ELISA指数优于IIF的平行性,并且ELISA指数可以预测病情是否会反复,从而指导治疗。结论:PV患者血清抗Dsg3抗体ELISA指数与病情变化平行波动,可以反映疾病的活动程度,可用于病情监测,并可为临床上预测疾病复发,指导临床调整治疗提供有利的实验室证据。  相似文献   

12.
目的 扩增人皮肤组织桥粒芯糖蛋白4(desmoglein 4,Dsg4)胞外区域EC1、EC2、EC3和EC4的核酸序列,为寻常型天疱疮(pemphigus vulgaris, PV)发病机制的研究提供依据.方法 根据GenBank中的Dsg4序列(登录号为AY177664)分析,通过逆转录聚合酶链反应(RT-PCR)法从人正常皮肤组织中抽提RNA,逆转录合成cDNA,聚合酶链反应(PCR)扩增皮肤组织桥粒芯糖蛋白4胞外区域EC1、EC2、EC3和EC4目的基因;应用基因重组技术将目的基因分别与质粒pET32a在T4 DNA连接酶作用下相连接,转化E.coli DH5α感受态细菌,用含氨苄青霉素的LB培养基平板筛选转化菌,阳性重组子DNA序列测定鉴定;重组融合蛋白经ELISA法与PV患者、正常对照组血清进行反应.结果 RT-PCR扩增产物经凝胶电泳得到均约为350 bp的4条条带;质粒载体pET32a连接的目的基因经序列测定后与在GenBank登录的Dsg4胞外区域EC1、EC2、EC3和EC4核酸序列完全一致,4个Dsg4胞外区域cDNA读码框架正确;Dsg4 EC1、EC2、EC3和EC4与患者血清反应,而不与正常对照组血清反应.结论 Dsg4胞外区域多肽片断在PV发病中可能有作用.  相似文献   

13.
高岩 《现代医学》2014,(6):648-651
目的:探讨天疱疮患者血清抗桥粒芯糖蛋白(Dsg)抗体与疾病临床特征的相关性。方法:选择49例天疱疮患者,比较其抗Dsg类别、亚型、滴度与疾病特征的相关性。结果:寻常型天疱疮(PV)患者活动期抗Dsgl、Dsg3抗体阳性率明显高于稳定期(P〈0.05),落叶型天疱疮(PF)患者活动期抗Dsgl抗体阳性率高于抗Dsg3抗体(P〈0.05);活动期患者两种抗体滴度随病情加重而明显升高(P〈0.05);活动期患者血清两种抗体IgG4亚型滴度明显高于IgG1,稳定期患者两种抗体IgG4亚型滴度明显下降且远低于IgG1(P〈0.05)。结论:天疱疮患者血清抗Dsg抗体与疾病表型、病情、活动性存在明显相关性,测定其存在和滴度对临床诊断与治疗有重要指导意义。  相似文献   

14.
利用PCR-Cloning技术和DNA重组技术,构建了两类IL-2-PE40'嵌合基因的表达载体,分别由IPTG和温度(42℃)诱导表达。在对表达产物进行了酶切位点和片段大小、电泳迁移率、分子量的初步鉴定后,又对融合蛋白的白细胞介素2(IL-2)和PE40'组分的抗原性进行了ELISA鉴定。从菌体蛋白中初步分离纯化了重组毒素,并测定其对细胞表面表达IL-2受体的活化T淋巴细胞的靶向杀伤效应及对混合淋巴细胞反应的抑制作用。结果表明,IL-2-PE40'融合蛋白确实对IL-2R+细胞具有明显的靶向杀伤效应并呈剂量依赖关系,与对照组相比,随着保温时间的延长(6、24、48h),杀伤效应趋于增强(P<0.05)。混合淋巴细胞培养结果显示,该融合蛋白对单相和双相反应均有不同程度的免疫抑制作用(P<0.01)。  相似文献   

15.
Zhang Y  Zhou ZG  Yang L  Zhou HF  Lin J  Sun Y  Huang G 《中华医学杂志》2007,87(16):1102-1105
目的探讨特发性1型糖尿病(1B型糖尿病)患者是否存在胰岛自身抗原反应性T细胞。方法选择经典1型糖尿病(1A型糖尿病)患者23例,1B型糖尿病29例,健康对照16例;放射配体法检测胰岛自身抗体;连续密度梯度离心法分离人外周血单个核细胞;酶联免疫斑点法(ELISPOT)检测谷氨酸脱羧酶(GAD65)反应性、分泌γ干扰素(IFN-γ)的T细胞(IFN-γ-T细胞)。结果(1)IFN-γ-T细胞数(中位数及95%CI表示)1A型糖尿病为:12.0个(10.3~20.9个),1B型糖尿病:3.5个(3.0~5.7个),对照:1.0个(0.3~1.8个)。1A型糖尿病的IFN-γ-T细胞数明显高于1B型糖尿病及对照(均P〈0.01)。1B型糖尿病较对照具有更高频率的IFN-γ-T细胞(P〈0.05)。(2)以〉95%CI(对照组)判为IFN-γ-T阳性,1A型糖尿病、1B型糖尿病及对照的阳性率分别为:78.3%(18/23)、48.3%(14/29)及0。(3)3组对植物血凝素非特异性刺激的IFN-γ-T细胞数差异无统计学意义(P〉0.05)。结论部分1B型糖尿病患者存在GAD65反应性IFN-γ-T细胞,即存在T细胞免疫异常,具有与1A型糖尿病相似的病因及发病机制;诊断1B型糖尿病前应排除IFN-γ-T阳性患者,GAD65 IFN-γ-T检测有望成为糖尿病分型诊断的新指标。  相似文献   

16.
人B7-2基因修饰的食管癌细胞瘤苗抗肿瘤的免疫效应   总被引:1,自引:2,他引:1  
目的:研究人B7-2基因修饰的食管癌细胞作为瘤苗的抗肿瘤作用。方法:将真核荧光表达载体pEGFP-C3-B7-2,通过脂质体转染技术转染人食管癌细胞株EC9706。外周血来源的树突状细胞(DC)负载肿瘤抗原后,与自体T淋巴细胞共培养3d,获得细胞毒性T淋巴细胞(CTL)。用四甲基偶氮唑蓝(MTT)法检测CTL对转染和未转染pEGFP-C3-B7-2的人食管癌细胞EC9706的杀伤活性。结果:CTL对转染pEGFP-C3-B7-2的肿瘤细胞的杀伤活性大于转染pEGFP-C3和未转染细胞的杀伤活性(P<0.05)。结论:人B7-2基因修饰的EC9706肿瘤细胞瘤苗,可诱导出明显的抗EC9706细胞的免疫效应。  相似文献   

17.
目的: 检测系统性红斑狼疮(systemic lupus erythematosus,SLE)患者中T和B淋巴细胞中穹窿核糖核酸 2-1(vault ribonucleic acid 2-1,VTRNA2-1)基因的表达水平,初步探索SLE的发病机制。方法: 收集25 例健康对照者 和32 例SLE患者的外周血CD4+ T淋巴细胞,另外再收集62 例SLE患者(其中活动性SLE患者47 例,非活动性患者15 例)和29 例健康对照者的外周血CD19+ B淋巴细胞,采用real-time PCR检测VTRNA2-1 基因的表达水平。采用免疫共 沉淀实验寻找VTRNA2-1 的直接作用蛋白。结果: 通过对SLE 患者和健康对照者外周血T 细胞和B 细胞中的 VTRNA2-1 基因进行检测,发现SLE患者T细胞中的VTRNA2-1 基因表达水平与健康对照者相比较,差异无统计学 意义(P>0.05)。活动性和非活动性的SLE患者外周血B细胞中的VTRNA2-1 基因表达水平与健康对照者相比均显著增 高,差异均有统计学意义(分别P<0.01 和P<0.05)。免疫共沉淀实验证实VTRNA2-1 通过与蛋白激酶R(protein kinase R,PKR)特异性结合而发挥生物学功能。结论: VTRNA2-1 基因在SLE患者的B细胞中呈高表达,其机制可能是通 过调控PKR而发挥生物学功能。  相似文献   

18.
寻常型天疱疮(PV)是一类危及生命的自身免疫性大疱性皮肤黏膜病,以往认为PV的发生与体液免疫有关,目前认为,除了B细胞的功能异常产生致病性抗体外,T细胞所介导的细胞免疫异常介导了PV患者出现病理性免疫应答。文章介绍了与PV发病相关的HLA-II类抗原,Dsg3特异性的自身反应性T细胞,以及调节性T细胞的最近研究发现。  相似文献   

19.
CLONINGANDEXPRESSIONOFTHEGENECODINGFORIL-2(60)-PE40,AMOLECULARTARGETEDPROTEINZhangMeng(张萌)ZhaoXue(赵雪)LiHuanlou(李焕娄)andLuSheng...  相似文献   

20.
目的:探讨何种细胞因子(IL-2或IL-7)及其最佳浓度能够最有效地改善酶联免疫斑点法(enzyme-linked immunospot,ELISPOT)检测1型糖尿病患者谷氨酸脱羧酶65(glutamic acid decarboxylase 65,GAD65)特异性T细胞反 应的效率。方法:选取20例1型糖尿病患者(A组)及年龄和性别均匹配的16例正常对照者(B组);Ficoll法分离外周血 单个核细胞(peripheral blood mononuclear cells,PBMCs),以人GAD65、内对照、巴斯德五合一疫苗为抗原,加入不 同浓度的IL-2[0 U/mL(1组)、0.5 U/mL(2组)、2.5 U/mL(3组)和12.5 U/mL(4组)],ELISPOT检测上述各浓度组分泌干 扰素- γ (interferon-gamma,IFN-γ)的CD4+T细胞,比较各浓度组的GAD65孔(信号)、内对照孔(背景)的斑点数、斑点 净值以及刺激指数(即信噪比,stimulating index,SI);另选取21例1型糖尿病患者(C组)及12例正常对照者(D组),检 测针对GAD65抗原的特异性T细胞反应,比较联合IL-2(2.5 U/mL,上述实验已证明其为最优浓度,5组)与联合IL-7 (0.5 ng/mL,6组)所产的斑点数和SI。结果:1)A组在加入IL-2刺激后,各浓度组中GAD65反应性T细胞数均较A1组增 高,而各浓度下的内对照孔的斑点数亦呈比例升高。各浓度组斑点净值比较显示:A4组与A3组差异无统计学意义 (P>0.05),而SI的比较则提示A3组最高;且仅A3组的SI(2.8)高于B3组(1.5),差异有统计学意义(P<0.05)。2)C6组及D6 组的GAD65孔斑点数均分别略高于C5组及D5组,且C6组及D6组的斑点数增幅也分别高于C5组及D5组,但差异均无 统计学意义(P>0.05)。C5组的斑点净值(5.5)和SI(2.8)均显著高于C6组的斑点净值(4.3)和SI(1.8),差异均有统计学意义 (均P<0.05)。结论:2.5 U/mL为ELISPOT检测1型糖尿病患者GAD65特异性T细胞反应的IL-2最佳浓度;IL-2较IL-7更有 利于改善ELISPOT检测的SI。  相似文献   

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