首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 46 毫秒
1.
《海南医学院学报》2017,(2):240-243
目的:探讨不同肺炎支原体(MP)感染病期婴幼儿免疫功能及炎症因子水平动态及其对患儿临床治疗意义。方法:选取2014年9月~2015年5月期间住院治疗的100例肺炎支原体肺炎(MPP)患儿为观察组,依据其感染程度分为MPP急性期和恢复期两组,同时选取同期健康体检儿童50例为对照组,对3组婴幼儿的免疫球蛋白(IgG、IgA、IgM)及补体(C3、C4)、外周T淋巴细胞亚群(CD3~+、CD4~+、CD8~+)及细胞炎症因子(IL-2、IL-6、TNF-α、IL-8、IL-10、IL-13)进行检测。结果:免疫球蛋白IgG恢复期水平明显高于急性期和对照组(P<0.05),急性期与恢复期IgA水平较对照组显著降低(P<0.05),急性期IgM及补体C3水平较对照组显著升高(P<0.05),在恢复期下降趋于正常,补体C4急性期水平均显著高于恢复期,均高于对照组(P<0.05)。MPP急性期和恢复期的CD3~+、CD4~+及CD4~+/CD8~+比值均明显低于对照组,且上述指标的恢复期数值显著高于急性期(P<0.05),CD8~+水平急性期高于恢复期,均显著高于对照组(P<0.05)。细胞炎症因子IL-2水平较对照组而言,急性期和恢复期水平均显著下降,且恢复期明显高于急性期(P<0.05),IL-6、TNF-α、IL-8、IL-10、IL-13水平检测结果急性期与恢复期水平均显著高于对照组,恢复期水平显著低于急性期(P<0.05)。结论:婴幼儿感染MP后,机体的体液免疫功能和细胞免疫功能损伤,使得机体免疫系统发生紊乱,同时炎症细胞因子水平表达异常。因此,实时监测各项指标的水平动态,可明确婴幼儿的病情,提高康复率,对婴幼儿临床诊断具有一定的指导意义。  相似文献   

2.
肺炎支原体肺炎免疫功能动态变化及临床意义   总被引:1,自引:0,他引:1  
目的探讨肺炎支原体肺炎(MPP)急性期和恢复期免疫功能动态变化及临床意义。方法肺炎支原体IgM检测采用ELISA法,免疫球蛋白IgA、IgG、IgM检测采用琼脂免疫扩散法检测。T淋巴细胞亚群采用APAAP法检测。sIL2R、IL6、IL8检测均采用双抗体夹心法。结果与对照组比较,MPP患儿急性期IgG、IgM、CD 8、sIL2R、IL6、IL8值均明显升高(P<0.01),CD 4、IL2值均明显下降(P<0.01),IgA、CD 3值均无明显改变(P>0.05);与急性期比较,MPP患儿恢复期IgA、IgG、IgM及T淋巴细胞亚群值无明显改变,而IL2值均明显升高(P<0.01),sIL2R、IL6及IL8值均明显下降(P<0.01);与对照组比较,MPP患儿恢复期IgG、IgM、CD 8仍均明显升高(P<0.01),CD 4明显下降(P<0.01),IL2、IL2R、IL6、IL8均无明显改变(P>0.05)。结论MPP患儿存在细胞免疫功能低下及体液免疫紊乱,IgG、IgM和T淋巴细胞的亚群恢复较慢,而IL2、sIL2R、IL6及IL8值恢复较快,检测MPP患儿免疫功能,特别是IL2、sIL2R、IL6和IL8水平对疗效及预后的判定有重要价值。  相似文献   

3.
儿童重型与轻型支原体肺炎免疫变化及临床意义   总被引:2,自引:0,他引:2  
目的 检测儿童重型肺炎支原体肺炎(MPP)与轻型MPP的体液免疫及细胞免疫状况,为临床免疫治疗寻找依据.方法 157例MPP患儿按病情分为轻症组(132例)和重症组(25例),以同期儿科门诊体检的健康儿童25例为对照组,检测上述所有患儿急性期免疫指标.结果 轻症组及重症组患儿IgG、IgM水平均高于对照组(均P <0.05),重症组患儿IgM较轻症组患儿水平高(P<0.05).两组MPP患儿IgA与对照组比较,差异均无统计学意义(P<0.05);轻症及重症组惠儿CD4+T细胞比例、CD4+/CD8+T细胞比值均低于对照组(均P<0.05),两组MPP患儿CD19+、CD8+T细胞比例均高于对照组,差异有统计学意义(P<0.05),且重症组CD19+T细胞比例高于轻症组,差异有统计学意义(P<0.05);重症组患儿CD3+、CD16+CD56+T细胞比例明显低于轻症组(均P <0.05).结论 MPP感染患儿B淋巴细胞异常活化且T淋巴细胞和自然杀伤细胞(NK)免疫功能低下,重症组更明显.免疫制剂对MPP患儿临床治疗有效,对重症MPP疗效更明显.  相似文献   

4.
目的探讨初诊肺炎支原体肺炎(MPP)患儿机体免疫功能指标的改变及临床意义。方法收集2018年6月至2019年3月在南京医科大学附属无锡人民医院儿童住院部初诊172例MPP患儿作为研究对象,其中轻症105例重症67例;同期选取性别、年龄相匹配的63名健康体检者作为对照组。流式细胞术检测各组外周全血T淋巴细胞亚群分子指标CD3~+、CD4~+、CD8~+、CD4~+/CD8~+,并采用免疫比浊法测定各组血清免疫球蛋白IgG、IgM、IgA的水平。结果轻症和重症MPP组CD3~+、CD4~+、CD4~+/CD8~+水平低于对照组,且重症MPP组CD3~+、CD4~+、CD4~+/CD8~+水平低于轻症组[(43.1±16.7)%比(55.3±18.8)%、(16.1±6.8)%比(24.3±8.1)%、0.64±0.28比0.97±0.29](P <0.05),各组CD8~+比较差异无统计学意义(P>0.05)。轻症和重症MPP组血清IgG、IgM、IgA水平均较对照组明显升高(P <0.05)。结论未接受药物治疗的初诊重症MPP患儿T淋巴细胞亚群CD3~+、CD4~+、CD4~+/CD8~+水平较健康对照和轻症患儿明显降低,Ig水平升高。初诊MPP患儿的免疫状态检测可为临床诊疗提供参考依据。  相似文献   

5.
目的 探究肺炎支原体感染后患儿血清免疫球蛋白及T淋巴细胞亚群的动态变化分析情况.方法 便利选取该院2014年4月-2016年4月期间确诊的肺部支原体感染患儿70例为观察组,选取同时期于该院体检的健康小儿45名为对照组,抽取两组研究对象外周血以免疫散射比浊法和流式细胞术进行检测.比较两组在IgG﹑IgM﹑IgA血清免疫球蛋白与CD3+﹑CD4+﹑CD4+/CD8+T淋巴细胞亚群的变化情况.结果 观察组患儿血清免疫球蛋白检测中IgG﹑IgM及IgA水平均低于对照组健康小儿.观察组急性期患儿血清免疫蛋白中IgG(9.12±1.48)g/L﹑IgM(0.76±0.41)g/L﹑IgA(1.03±0.19)g/L与对照组相比差异有统计学意义(P<0.05),且观察组急性期IgG﹑IgM﹑IgA水平略低于恢复期(P<0.05).②观察组患者各时期T淋巴细胞亚群中CD3+﹑CD4+﹑CD4+/CD8+均分别低于对照组,差异有统计学意义(P<0.05).观察组各时期CD8+(34.27±3.48)%均高于对照组,且观察组急性期CD3+(59.56±7.34)%﹑CD4+(40.38±7.23)%﹑CD4+/CD8+(1.14±0.53)%与恢复期比较差异有统计学意义(P<0.05).结论 小儿肺炎支原体感染后,在急性期与恢复期的血清免疫球蛋白及T淋巴细胞亚群均出现不同幅度的变化,MP与机体免疫反应存在相关,可据此做出相对应的MP诊治方案.  相似文献   

6.
目的探讨高敏C-反应蛋白(hs-CRP)对肺炎支原体感染患儿病情的评估价值。方法选取该院儿科收治的肺炎支原体肺炎(MPP)患者115例,按是否全身炎症反应综合征(SIRS)分为SIRS组和非SIRS组,另取同期30例健康体检儿童作为对照,检测白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、高敏C-反应蛋白(hs-CRP)、黏膜局部抗体(IgA)、免疫球蛋白G(IgG)和免疫球蛋白M(IgM)、T淋巴细胞CD3~+、CD4~+、CD8~+及CD4~+/CD8~+等指标。结果 MPP患儿的IL-6、IL-8、TNF-α、hs-CRP、IgG、IgM和CD8~+水平显著高于对照组(P<0.01),且SIRS组的IL-6、IL-8、TNF-α、hs-CRP、IgG和IgM水平显著高于非SIRS组(P<0.05)。所有MPP患儿的IgA、CD3~+、CD4~+及CD4~+/CD8~+水平显著低于对照组(P<0.05),且SIRS组的IgA、CD3~+、CD4~+及CD4~+/CD8~+水平显著低于非SIRS组(P<0.05)。结论 MPP患儿的免疫功能受到损害,其程度与MPP患儿病情严重度密切相关;血清hs-CRP水平与细胞因子和免疫蛋白水平密切相关,可以反映机体免疫功能的状况,协助判断MPP患儿病情程度。  相似文献   

7.
目的:探讨肺炎支原体肺炎(MPP)患儿细胞免疫、体液免疫变化,以期能为临床免疫治疗提供理论参考依据。方法:选取急性期MPP患儿196例(MPP组),其中轻型152例,重型44例,选取同期外科择期手术7岁以下儿童50例为对照组,检测IgA、IgG、IgM水平及CD3^+、CD4^+。结果:MPP组IgG、IgM水平高于对照组,比较差异具有统计学意义(P〈0.05),MPP组轻型患儿XgG、IgM水平均高于对照组,重型患儿IgM水平高于轻型患儿及对照组,比较差异具有统计学意义(P〈0.05);MPP组CD3^+、CD4^+均低于对照组,比较差异具有统计学意义(P〈0.05),MPP组轻型患儿CD4^+低于对照组,重型患儿CD4^+低于对照组,CD3^+低于对照组及轻型患儿,比较差异具有统计学意义(P〈0.05)。结论:MPP感染患儿存在B淋巴细胞异常活化及T淋巴细胞低下,重型肺炎患儿表现更明显。  相似文献   

8.
毛国庆  冯丽 《实用医技杂志》2007,14(32):4425-4426
目的:探讨肺炎支原体肺炎(MPP)患儿血清白细胞介素23(IL-23)和免疫球蛋白(immunoglobulin,lg)在支原体肺炎发病过程中的临床意义。方法:IgG、IgA、IgM检测采用速率散射比浊法,IL-23检测采用双抗体夹心ELISA法。结果:与对照组相比,患儿急性期血清中IL-23水平显著升高,P<0.01,恢复期和急性期相比,患儿血清中IL-23含量有所下降但仍高于对照组,P<0.01。IgG、IgM、IgA恢复期高于急性期及对照组,P<0.05,急性期与对照组之间差异无显著性P>0.05。结论:免疫损伤在儿童MPP的发病机制中起着重要作用,IL-23和Ig参与MPP的免疫发病机制。  相似文献   

9.
目的探讨儿童肺炎支原体肺炎外周血T淋巴细胞亚群、细胞因子以及免疫球蛋白的动态变化。方法选取2018年12月至2020年5月本院收治的儿童支原体肺炎患儿50例作为观察组,按病情的发展进程分为急性期组和恢复期组,各25例;另选取同期健康儿童50名作为对照组。3组均进行样本采集,比较3组外周血T淋巴细胞亚群、细胞因子以及免疫球蛋白的动态效果。结果检测后,急性期组CD3+、CD4+、CD4+/CD8+均低于恢复期组和对照组(P<0.05),NK细胞的比例高于恢复期组和对照组(P<0.05),恢复期组CD8+与B细胞的占比与对照组比较差异无统计学意义。检测后,急性期组IFN-γ、IL-4、Th1/Th2含量均高于恢复期组和对照组(P<0.05),恢复期组IFN-γ、IL-4、Th1/Th2与对照组比较差异无统计学意义。检测后,恢复期组血清IgG水平高于急性期组和对照组(P<0.05),急性期组血清IgG水平与对照组比较差异无统计学意义,急性期组和恢复期组血清IgA水平均低于对照组(P<0.05),急性期组血清IgA水平与恢复期组比较差异无统计学意义,急性期组血清IgM水平高于对照组和恢复期组(P<0.05),对照组血清IgM水平与恢复期组比较差异无统计学意义。结论患儿感染支原体肺炎时,机体内免疫功能发生紊乱,随病情的发展,免疫状态发生变化,临床上对幼儿采取免疫监测,可对疾病的诊断以及后期治疗提供依据。  相似文献   

10.
目的 探讨再生障碍性贫血 (再障 )患者T淋巴细胞亚群与免疫球蛋白的变化及其临床意义。方法 对 2 2例再障患者 (再障组 )进行外周血T淋巴细胞亚群及其血清免疫球蛋白检测 ,并与 18例门诊健康体检者 (对照组 )进行比较。结果 再障组CD+ 4 、CD+ 4 /CD+ 8、IgG、IgA、IgM水平比对照组明显降低 (P均 <0 .0 1) ,但CD+ 8明显升高 (P <0 .0 1) ;大部分患者CD+ 3 正常。再障组治疗后 15例有效 ,其IgG、IgA、IgM、CD+ 4 、CD+ 4 /CD+ 8比值升高至正常或接近正常 ,CD+ 8明显降低至正常或接近正常 ,与治疗前相比差异有显著性 (P <0 .0 1或 0 .0 5 )。结论 再障患者发病与免疫功能异常密切相关 ,不仅存在细胞免疫功能的异常 ,也存在着体液免疫功能的异常。用免疫抑制剂治疗有效者 ,T淋巴细胞亚群、免疫球蛋白水平可恢复正常或接近正常  相似文献   

11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

12.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

13.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

19.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

20.
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号