三七乙醇提取工艺优选

目的:优选三七的最佳提取工艺。方法:采用正交试验法,以三七总皂苷的含量作为考察指标,优选三七的提取工艺条件。结果:优选的工艺为粉碎成最粗粉,加7倍量60%乙醇浸泡过夜,回流提取3次,每次1.0 h。结论:优选的提取工艺三七总皂苷提取率高,可为三七提取工艺的确定提供实验依据;该优化工艺可为工业生产提供参考。     

复方祛疤凝胶中三七提取工艺的研究

目的：筛选复方祛疤凝胶中三七的最佳提取工艺。方法：通过前期对提取方法的考察,确定了复方祛疤凝胶中三七应采用回流法进行提取,在本研究中通过正交试验设计,以三七皂苷R1、人参皂苷RgMl、人参皂苷Rb1的含量及干膏率为考察指标,进行多指标综合评分,最终筛选出复方祛疤凝胶中三七的最佳提取工艺。结果：三七的最佳提取工艺为：加1...     

参草通脉颗粒治疗心衰的三七成分提取研究

目的：参草通脉颗粒在临床中治疗慢性心衰疗效显著确切,能明显改善病人症状,降低再住院率等。为了进一步明确中药的疗效成分便于推广研究。我们研究其主要药物三七中主要成分三七皂苷的最佳醇提取工艺条件,为参草通脉颗粒治疗慢性心衰中三七药材综合利用提供实验依据。方法：采用正交试验法,以三七总皂苷的提取量及浸膏得率为考察指标,优选回流法提取的最佳工艺条件。结果：最佳乙醇回流工艺为：以8倍量70％乙醇回流提取4次,每次1．5h。结论：参草通脉颗粒三七优选的提取工艺设计合理,三七总皂苷提取率较高。     

正交设计优化三七总皂苷提取工艺

目的优化三七中三七总皂苷的提取工艺。方法采用紫外分光光度法,以提取物中三七总皂苷含量为考察指标,应用正交实验法对提取工艺进行优化。结果以70%的6倍量乙醇为提取溶媒,回流3次,每次60min,经纯化后三七总皂苷含量达70%以上。结论该工艺简便易行,提取率高,生产成本低,适合工业生产。     

正交试验法研究三七提取工艺

目的:优选三七的最佳提取工艺,方法:采用正交试验法,以提取注保三七总皂苷含量为考察指标,对影响三七提取工艺的因素进行了研究.结果:实验设计三因素中提取方式有显著影响.结论:三七的最佳提取工艺为:用７５%乙醇浸渍三七２４h后,以每公斤每分钟１-３mL速度渗漉,收集相当于三七１０倍量的渗漉液.     

正交优选白背三七茎总黄酮提取工艺研究

目的采用正交试验法对白背三七茎总黄酮的提取工艺进行优选。方法以紫外分光光度法为检测方法、总黄酮得率为优选指标,采用L9(34)正交试验法考察料液比、乙醇浓度、提取时间、提取次数4个影响因素对总黄酮提取的影响。结果最佳提取工艺为料液比1∶8、乙醇浓度60%,回流提取3次,每次1 h。结论该工艺可为白背三七的开发利用提供参考。     

酶转化三七叶总皂苷制备人参皂苷C-K的工艺优化

目的 研究以三七叶总皂苷制备人参皂苷C-K的酶转化工艺。方法 利用4种工业酶制剂对三七叶总皂苷进行转化，筛选出最佳酶制剂；以人参皂苷C-K的含量为指标，采用正交试验法进行酶转化工艺的优化；采用TLC及HPLC法检测酶转化产物。结果 酶转化三七叶皂苷的最佳工艺条件为：20％p葡聚糖苷酶，pH5．4，反应温度55℃，反应时间48h。反应时间的影响最大，其次是酶的体积分数。结论 β-葡聚糖苷酶对三七叶总皂苷的转化作用最强。     

三七中人参皂苷Rg1的提取及含量测定

目的：测定三七中人参皂苷Rg1含量,优化三七皂苷的超声提取工艺。方法：高效液相色谱法测定人参皂苷Rg1的含量,色谱条件为：C18柱（4.6 mm×200 mm,5μm）,以乙腈-蒸馏水（24∶76）为流动相,检测波长为203 nm,流速为1.0 ml/min。以人参皂苷Rg1为指标,正交试验优化三七皂苷的提取工艺。结果：人参皂苷Rg1进样量在0.312～2.496μg范围内呈良好线性关系,平均回收率为100.8%,三七的最佳提取条件是10倍量60%乙醇超声30 min。结论：本法提取率较高,含量测定方法准确可靠。     

三七皂苷加压提取工艺优化与抗氧化活性研究

目的考察加压提取三七皂苷的最佳工艺参数,并对其抗氧化活性进行研究。方法以三七皂苷有效成分提取率为指标,采用正交试验法对提取压力、温度、时间、乙醇浓度、粒径、料液比等因素条件进行优选,确定最佳提取工艺,同时采用DPPH法研究加压提取三七皂苷有效成分的体外抗氧化活性。结果加压提取三七皂苷的最佳工艺为:三七药材,粉碎,过60目筛,30倍量80%乙醇,在提取温度为100℃、提取压力为0.6 Mpa条件下,提取30 min,此条件下提取的三七皂苷在30~90μg/m L内,抗氧化能力随着浓度的升高而增强。结论三七皂苷具有很强的自由基清除能力,加压提取三七皂苷经济、合理、简便,且不会影响其抗氧化能力。     

正交实验优选三七总皂苷的提取工艺研究

目的：采用正交实验设计优化三七中三七总皂苷的提取工艺。方法：以总皂苷转移率、提取物中皂苷含量和浸膏得率为指标,采用综合评分法考察提取溶剂、溶剂药材用量比和提取次数三个因素对三七总皂苷提取率的影响。结果：8倍量60％的乙醇提取三七药材粉末2次,每次2h,可将其中的总皂苷提取完全,且所得浸膏皂苷含量较高。结论：该工艺切实可行,可节约了溶剂消耗。     

中西医结合治疗难治性心衰34例——附西药对照组30例

在西药治疗的基础上,加用黄芪注射液和川芎嗪注射液、静脉滴注,治疗难治性心衰３４例,并与西药治疗的３０例相对照。结果表明：治疗组显效１１例,有效１９例,无效４例;对照组显效３例,有效１７例,无效１０例。治疗后在血液动力学、流变学方面,治疗组绝大多数指标均得到显著或极显著改善（Ｐ＜０．０５～０．０１）,对照组各项指标几无明显改善（Ｐ＞０．０５）。     

湖北产四种商品细辛的鉴别

对湖北产四种商品细辛的性状及显微特征进行了比较研究。并将其主要区别点列表说明。为湖北地区细辛的生产，销售及临床应用提供参考。     

珠子参地下及地上部分的显微鉴别研究

目的：通过对珠子参地上部分及地下部分的显微鉴别研究，为珠子参及其同属植物的鉴别提供依据。方法：采用显微鉴别方法对其地下及地上部分作了系统的研究，分别对地下部分的节和节间横切面、粉末，地上部分的茎、叶横切面、粉末及叶片的上下表面观等显微特征进行了描述。结果：详细描述了珠子参各部分的显微特征。结论：本研究不仅提高和完善了珠子参地下部分的显微研究，而且为珠子参地上部分的质量标准研究、开发利用、扩大药用资源提供了理论依据。     

硫氟酰胺的合成和药效观察

以辛烷磺酰氟为原料经电解氟化和胺化反应合成了硫氟酰胺化合物,它们对德国小蠊有良好的杀灭效果。     

一例血红蛋白G—Taipei的结构分析

用醋酸纤维素薄膜电泳在河南新乡普查血红蛋白病时,发现一例慢速血红蛋白变种。先证者21岁,女,河南籍,体健。其父携带有相同泳速的异常血红蛋白。化学结构分析,证明该变异体为HbG—Taipei[β22(B4)Glu—Gly]。     

阻抗心动图在临床及麻醉中的应用

阻抗心动图具有无创伤、简单、安全、重复性好、能连续观察等特点,为临床提供了新的心功能监测方法。本文简述了它的基本原理、波形的意义及在临床和麻醉中的应用。     

某化工总厂铅作业职业危害调查

本文通过对某化工总厂的劳动卫生学调查,探讨了铅对工人健康的危害及其浓度与危害之间的关系。发现作业场所铅尘浓度最高达44.37mg/m~3。超过国家规定的卫生标准886.4倍。平均超标率为84.21％。34名工人尿铅均值为0.116mg/L,超标率为61.76％。铅中毒检出率为22.22％。铅吸收检出率为8.33％。结果表明:(1)尿铅含量与接触空气铅浓度高低有关。铅作业工令越长,铅中毒检出率越高。(2)慢性铅吸入对血压影响不明显。(3)该厂铅危害是比较严重的,在县以下工厂企业中具有一定的代表性,应引起足够的重视。     

荞麦花叶总黄酮抗应激反应的实验研究

①目的研究荞麦花叶总黄酮（TFBFL）对小鼠抗急性应激反应的能力。②方法将小鼠随机分为正常对照组及TFBFL高、低剂量组。TFBFL组按40mg／（kg·d）和20mg／kg·d），灌胃给药7天，正常对照组灌胃等容量的去离子水。未次给药2小时，采用负重游泳法，观察游泳时间；常压耐缺氧和耐低温法，观察小鼠存活时间。③结果TFBFL能延长小鼠负重游泳时间，缺氧存活时闾及提高耐寒能力，TFBFL高剂量作用更为显著。④结论TFBFL具有明显的抗疲劳、耐缺氧、耐低温作用，可增加小鼠应激反应的能力。     

千斤拔的品种调查与质量研究

对千斤拔的品种调查表明,蔓性千斤拨、大叶千斤拔和宽叶千斤拨是当前的主流品种,经对３种千斤拔药材性状,组织结构、化学成分预试,ＴＬＣ、ＵＶ比较,以及浸出物、总黄酮含量测定,急性性与药效学试验,结果表明３才所含成分大致相似,经理作用无明显差异,认为可等同入药。     

53例大型、巨大型蝶骨嵴脑膜瘤临床特征及治疗体会

Background Large and giant medial sphenoid wing meningiomas located deeply in the skull base where cavernous sinus, optic nerve and Internal Carotid Artery(ICA) were closely bounded, which made the gross resection hard to achieve, also, this kind of meningiomas often accompanied with a series of severe complications. Therefore, it was regarded as a formidable challenge to even the most experienced neurosurgeons. Methods In this study, 53 patients with large and giant medial sphenoid wing meningiomas were treated surgically between April 2004 to March 2012, with their clinical features analyzed, management experience collected, treatment result investigated retrospectively, and that allowed us to reach the most preferable and comprehend management way. There were 33 female and 20 male (mean age 47.5 years). The most frequent symptoms were headache, and decreased visual acuity. 35patients (66.0%) had large tumor which meant the average diameter of tumors was around 6.0 cm, the other 18 patients (34.0%) had giant tumor with the mean diameter of 8.1 cm. The bases of these tumors were mainly located around the anterior clinoid process. Image study showed that ICA was affected in all 53 patients. Orbitozygomatic approach was performed on 18 patients (34.0%), while the Frontotemporal (modified pterion) approach was applied on the other patients (66.0%), the medial sphenoid wing crest and anterior clinoid was stripped in 33 patients. Result In this study, Gross Total Resection(GTR)(SimpsonⅠ,Ⅱ) was applied in 44 patients (83.0%). 53 patients had accept the routine Computer Tomography (CT) scan, and a Magnetic Resonance Imaging (MRI) scan as the postoperative neuroradiological evaluation. Their performance showed surgical complications of vascular lesions and helped to evaluate patients’ condition respectively. Meanwhile, the drugs resisting cerebral angiospasm such as Nimodipine was infused for every postoperative patient through vein as routine. As a result, 11 patients (20.7%) with secondary injury of cranial nerve Ⅱ, Ⅲ and Ⅳ and 9 patients got recovered during long-term observing follow-up period. Temporary surgical complication of vascular lesion occurred after surgery, such as cerebral angiospasm and ischemia, edema, 24 patients (45.3%) in total appeared to have infarction and dyskinesia of limbs. Overall visual outcome were improved in 41 patients (77.4%). No patient died in the process. Conclusion These demonstrated that microsurgical treatment was the most effective method for the large and giant medial sphenoid wing meningiomas. And the surgical strategy should focus on survival and postoperative living quality. On this aspect, approaches’ choosing should be based on anatomic characters, doctor’s operative skill, tumor’s position, tumor’s growth direction, tumor’s invasion on adjacent structure, and the experience of neurosurgeons. It is an effective method in decreasing bleeding by dealing with tumor base firstly during the operation. Debulking in giant tumor is also good choice in tumor exposure. Mapping the optic nerve and the ICA was also a key factor in a success operation. If embedded by the tumor, the vessels could be seperated from the tumor by mode of proximal-to-distal along the trunk to the tiny branches, and it is also an effective method in vessel protection. Complete excision is always recommended, but surgeons should acknowledge that they should weigh the recurrence against the risk of causing cranial nerve injuries.