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1.
本文检测了一组再生障碍性贫血患者血浆和细胞诱生的肿瘤坏死因子(TNFa)和细胞诱生上清的白细胞介素2(IL-2)。与正常对照比较,再障病人血浆和细胞诱生上清的TNFa活性水平明显增高(P<0.01),IL-2的诱生水平也明显增高(P<0.05),本研究提示TNFa和IL-2在再障的发病中可能有一定的作用。  相似文献   

2.
为了解甘草甜素对慢性活动性肝炎(CAH)患者外周血单个核细胞(PBMC)产生的肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)的影响,用强力宁(主要成分为甘草甜素)对18例CAH患者PBMC产生IL-6、TNF-α的能力进行了研究。结果显示在体外培养条件下,强力宁可使PBMC产生IL-6、TNF-α的能力减弱;而且PBMC产生IL-6、TNF-α的能力降至最低(接近正常)时所需的剂量在CAH活动期明显低于稳定期(P<0.01)。提示用强力宁治疗CAH时应根据不同病程调节剂量。研究尚发现强力宁可能具有诱生PBMC膜产生可溶性IL-6受体的作用,从而中和了培养上清液中的IL-6。上述结果可部分解释其治疗CAH的机理。  相似文献   

3.
目的:探讨血清中可溶性白细胞介素-2受体(sIL-2R)和肿瘤坏死因子α(TNF-α)水平及其在检查丙肝病毒感染中的价值。方法:应用ELISA方法检测42例正常人及87例丙肝病毒感染者血清中sIL-2R和TNF-α水平。另外,将感染者分为3组,即A组(抗-HCV-Ab(-),HCV-RNA(+)),B组(抗-HCV-Ab(+),HCV-RNA(-)),C组(抗-HCV-Ab(+),HCV-RNA(+))。结果:87例感染者血清中sIL-2R和TNF-α水平较正常人都明显增高P<0.001),所有组的血清中sIL-2R和TNF-α水平也与正常人差异显著(P<0.01)。不同组彼此间比较sIL-2R和TNF-α水平,除A组与C组外,A组和B组之间、B组和C组之间都有显著差异(P<0.001~0.05)。结论:对sIL-2R和TNF-α水平的检测可作为丙型肝炎辅助化验检查指标之一。  相似文献   

4.
目的 观察HDV(+)重型肝炎外周血中TNF、IL-1及IL-6变化状况。方法 分离16例HDV(+)/HBV(+)重型肝炎血清和PBMC培养上清,EIA法则TNF,IL-1参照Mizel法,IL-6参与OkudaMuraguchi,以21例HDV(-)/HBV(+)重型肝炎为疾病对照,20例献血员为正常对照。结果 两且重型肝炎患者血浆及PBMC上清中TNF、IL-1及IL-6均非常显著升高,与献  相似文献   

5.
体重2.5~3.0kg日本大耳白兔,分为输精管结扎组(VG)和假手术组(SOG)。术后4、8、12、18、22月经耳静脉采血,玻璃平皿吸附法纯化巨噬细胞,经LPS诱生,测巨噬细胞培养上清白细胞介素1(IL-1)活性,及血浆肿瘤坏死因子(TNF)和溶菌酶含量。结果表明,VG8月IL-1活性和TNF、溶菌酶含量均明显高于SOG8月(P<0.02,<0.0l,<0.05),其它各月检测结果组间比较均无显著差异(P>0。05)。IL-1活性和溶菌酶含量呈明显的正相关关系(r=0.576,P<0.001)  相似文献   

6.
用黄连、黄柏、黄芩和金银花复方水煎剂治疗烧伤大鼠创面感染,测定血浆中白细胞介素6(IL-6)的含量和混和淋巴细胞培养上清液中的肿瘤坏死因子TNF诱生量。大鼠随机分成假烫组、烧伤创面感染对照组(烧感组)和烧伤创面感染十中药治疗组(中药组)。烧感组和中药组在伤后8、24和48h抽血查上述指标。结果示烧伤创面感染的大鼠自烧伤后24h起血浆IL-6均高于正常,烫伤后48h烧感组血浆IL-6显著高于中药组(P<0.01)。TNF的诱生量从伤后24h起,中药组显著高于烧感组,中药组伤后24和48hTNF的诱生量分别为4.2±0.9ng/ml和2.61±1ng/ml;烧感组分别为1.1±0.3ng/ml和0.9±0.3ng/ml(P<0.01)。由此说明抑制内源性致热因子IL-6和TNF的产生是中药清热解毒的重要机制。  相似文献   

7.
目的:观察银耳多糖(TP)对小鼠脾细胞IL-2、IL-6和TNF活性及其mRNA表达的影响。方法:分别用CTLL、7TD1和WEHI-164细胞株测小鼠脾细胞IL-2、IL-6和TNF-α活性,用Northerrnblot印迹杂交法测上述细胞因子的mRNA表达。结果:银耳多糖(TP)100ms/L可促进ConA诱导的脾细胞培养上清1L-2的活性,该作用12h即可出现,24h达到高峰。单用仅呈现类似但较弱的作用。TP100mg/L从12h起明显增强LPS活化的小鼠腹腔巨噬细胞产生IL-6的能力。TP25、100mg/L可明显增强小鼠腹腔巨噬细胞TNF-α的活性。TP可促进小鼠脾细胞中细胞因子IL-2、IL-6TNF-amRNA的表达量,并且具有一定的剂量反应关系和时效关系,最适剂量为200mg/kg,给药9d,表达量最高。结论:TP通过促进IL-2、IL-6和TNF-amRNA表达,而促进上述细胞因子的生成。  相似文献   

8.
为探讨肿瘤坏死因子(TNF)和白介素2(IL-2)在非胰岛素依赖型糖尿病(NIDDM)中的变化,我们检测了80例NIDDM患者血清TNF和IL-2含量,与正常对照组比较,发现NIDDM患者TNF水平显著增高(P<0.0001),IL-2浓度显著降低(P<0.0001)。患者的血清TNF、IL-2水平与其空腹血糖、体重指数、舒张压、眼、肾病变及是否应用胰岛素无显著相关关系。NIDDM患者血清TNF、IL-2水平异常,提示可能有免疫调节功能紊乱。  相似文献   

9.
为探讨中药复方制剂通痹灵(以桂枝芍药知母汤为基础)对佐剂性关节炎大鼠(AA)滑膜细胞分泌白细胞介素1(IL-1)、肿瘤坏死因子α(TNF-α)和前列腺素E2(PGE2)的影响,采用大鼠膝关节滑膜细胞原代培养的方法,检测脂多糖(LPS)诱导关节滑膜细胞培养上清中IL-1、TNF-α的活性和PGE2的含量。结果:AA大鼠滑膜细胞培养上清中IL-1、TNF-α的活性和PGE2的含量高于正常组(P<0.01);通痹灵可使AA大鼠滑膜细胞异常升高的IL-1、TNF-α和PGE2分泌功能恢复正常(与AA组比P<0.01,与正常组比P<0.05);甲氨喋呤(MTX)也在一定程度上下调了滑膜细胞的分泌功能(与AA组比P<0.01,与正常组比P<0.05);消炎痛对PGE2的产生具有明显的抑制作用(与AA组比P<0.01,与正常组比P<0.01),但进一步上调了AA大鼠滑膜细胞分泌IL-1、TNF-α的功能(与正常组比P<0.01)。结论:IL-1、TNF-α和PGE2在类风湿性关节炎(RA)发病机理中占有重要地位;通痹灵对RA的治疗作用可能与其抑制滑膜细胞过度分泌细胞因子及炎症介质有关。  相似文献   

10.
目的探讨可溶性白细胞介素2受体(sIL-2R)、肿瘤坏死因子α(TNFα)及其可溶性受体Ⅰ型(sTNF-RⅠ)在皮肤T细胞性淋巴瘤(CTCL)发病机制中的作用。方法采用单克隆和多克隆双抗体夹心法,测定CTCL患者和对照组血清标本中sIL-2R、TNFα和sTNF-RⅠ,同时测定血清乳酸脱氢酶(LDH)水平。结果CTCL患者血清sIL-2R水平明显高于对照组(P<0.01),同时sIL-2R增高的比例在不同的临床分期中也不同,其中,血清中sIL-2R在Ⅳ期病人中明显高于Ⅰ、Ⅱ期(P<0.05)。治疗有效时,sIL-2R水平下降。sIL-2R水平与血LDH无相关性。TNFα水平明显高于正常对照组(P<0.01),sTNF-RⅠ除个别升高外,大部分在正常范围内,且与分期无相关(P>0.05)。结论测定血清sIL-2R可作为CTCL患者病情判断、疗效观察及估计预后的生物学指标。而TNF的升高,表明细胞因子参与了肿瘤的发生和发展。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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