病毒感染与肺癌发病的关系研究

目的:探讨肺癌患者血液及癌组织中EB病毒的感染状况,研究EB病毒感染与肺癌的相关性。方法:应用ELISA方法和PCR技术对40例肺癌患者的血液及新鲜肺癌组织标本进行EB病毒检测,对数据资料进行统计学处理。结果:肺癌患者血液EB病毒VCA-IgG抗体检出率高于非癌胸部疾病患者(P<0.01)。同病理类型肺癌EB病毒感染率无显著性差异(P>0.05)。结论:EB病毒感染可能与肺癌发生有关。     

病毒感染与肺癌发病的关系研究

目的：探讨肺癌患者血液及癌组织中EB病毒的感染状况，研究EB病毒感染与肺癌的相关性。方法：应用ELISA方法和PCR技术对40例肺癌患者的血液及新鲜肺癌组织标本进行EB病毒检测．对数据资料进行统计学处理。结果：肺癌患者血液EB病毒VCA-IgG抗体检出率高于非癌胸部疾病患者（P〈0．01）。同病理类型肺癌EB病毒感染率无显著性差异（P〉0．05）。结论：EB病毒感染可能与肺癌发生有关。     

肺癌患者咽部和癌组织中EB病毒感染情况及其关系

目的:探讨肺癌患者咽部分泌物、癌组织中EB病毒感染情况与肺癌的关系.方法:应用PCR技术对采自30例进展期肺癌患者的咽部分泌物及新鲜肺癌组织标本进行EB病毒检测.结果:肺癌患者咽部分泌物EB病毒感染率低,差异无显著性(P>0.05);癌组织中EB病毒感染率为43.3%;不同病理类型肺癌EB病毒感染率差异无显著性;肺癌患者咽部分泌物及癌组织EB病毒感染率之间无相关性.结论:进展期肺癌患者咽部EB病毒感染无临床意义;肺癌患者癌组织中EB病毒感染存在增高现象,EB病毒长期感染与肺癌关系密切.     

血浆中EB病毒核酸载量与鼻咽鳞状细胞癌分化的关系

目的 探索血浆中EB病毒的定量与鼻咽鳞状细胞癌分化的关系.方法 收集组织学为鳞状细胞癌的鼻咽癌38例,运用免疫荧光法测定血浆中EB病毒DNA水平和外周血CEA含量.结果 不同分化组患者血浆EB病毒定量有统计学差异（P=0.018）.不同分化组患者血CEA无统计学差异（P=0.806）.结论 血浆中EB病毒DNA水平与鳞状细胞癌分化关系密切,血浆中EB病毒定量与组织中的EB病毒RNA水平可能存在着某种关联,并且由此影响着疾病的转归和发展.     

三种疱疹科病毒潜伏感染与肺癌的关系研究

目的：探讨三种疱疹科病毒与肺癌的关系。方法：取肺癌、癌旁正常组织和非特异性炎症肺组织石蜡包埋,应用PCR方法测定EB病毒（EBV）、巨细胞病毒（CMV）和人类疱疹病毒6型（HHV-6）DNA,分析三种疱疹科病毒与肺癌的关系。结果：PCR检测EBV、CMV、HHV-6结果,肺癌组织分别为58.4%、33.3%、38.9%,癌旁组织为45.5%、36.4%、30.3%,非特异性炎症肺组织为36.4%、18.2%、27.3%。同癌旁组织比较,肺癌组织中EBV具有较高的感染率（P〈0.01）,而CMV及HHV-6无显著性差异（P〉0.05）;同炎症组织比较,三种病毒均有较高的感染率（P〈0.01）,癌旁组织与炎症组织比较,三种病毒亦有较高的感染率（P〈0.01）。结论：EBV感染与肺癌的发生有关,肺癌组织中EBV感染率明显高于癌旁组织及非特异性炎症肺组织。     

肝细胞癌中潜伏膜蛋白-1的表达及意义

目的 探讨EB病毒潜伏膜蛋白-1(LMP1)在肝细胞癌(HCC)中的表达及意义.方法 应用免疫组织化学SP法,对石蜡包埋的50例HCC组织(HCC组)和10例正常肝组织(对照组)标本检测LMP1的表达.结果 (1)LMP1阳性表达率HCC癌组织(38%)和癌旁肝硬化组织(38.9%)均高于癌旁非肝硬化组织和正常对照组(均为0,P＜0.05);(2)HCC癌组织中LMP1阳性表达率在Ⅲ～Ⅳ(51.7%)高于Ⅰ～Ⅱ期(19.0%,P＜0.05),有门静脉癌栓形成(50%)高于无门静脉癌栓形成(16.7%,P＜0.05).结论 EB病毒LMP1与HCC病期进展及有无门静脉癌栓形成密切相关,可作为估计HCC患者预后的参考指标.     

胃癌患者EB病毒感染与Cyclin D1基因表达的相关性

目的:研究胃癌患者癌组织中EB病毒(EBV)感染与Cyclin D1表达的关系. 方法:原位杂交法检测胃癌患者石蜡标本中EBV编码的小RNA(EBER1);免疫组化法检测EBV阳性胃癌[EBV( )] 及EBV阴性胃癌[EBV(-)]标本细胞周期蛋白Cyclin D1的表达状况. 结果:胃癌患者癌组织标本217例中23例EBER1( ); EBV( )组Cyclin D1蛋白表达显著高于EBV(-)组(P＜0.01). 结论:EBV感染可能上调Cyclin D1表达.     

肺癌组织中EBV感染与P53表达研究

目的　研究肺癌组织中EBV感染与P5 3表达的关系。方法　应用免疫组化方法检测 73例肺癌组织 ,其中鳞癌3 9例 ,腺癌 18例 ,大细胞癌 9例 ,小细胞癌 5例、腺鳞癌 2例 ,另选 10例尸检肺组织作对照。结果　EBV免疫组化结果 :总阳性率为 5 6.1% (4 1/ 73 )。其中鳞癌 3 8.40 % (2 8/ 73 ) ,腺癌 8.2 % (6/ 73 ) ,大细胞癌 5 .5 % (4 / 73 ) ,小细胞癌 1.4% (1/73 ) ,腺鳞癌 2 .7% (2 / 73 )。 10例尸检肺组织中 1例EBV阳性 ,与肺癌组织的阳性率差异有显著性 (P <0 .0 5 )。P5 3免疫组化结果 :总阳性率 67.1% (4 9/ 73 ) ,其中鳞癌 3 9.7% (2 9/ 73 ) ,腺癌 15 .1% (11/ 73 ) ,大细胞癌 6.8% (5 / 73 ) ,小细胞癌 4.1% (3 / 73 ) ,腺鳞癌 1.4% (1/ 73 )。 10例尸检肺组织P5 3蛋白均阴性。在 41例EBV阳性表达的肺癌中有 3 6例P5 3阳性(87.8% ) ,两者间表达具有一致性。结论　EB病毒与肺癌关系密切 ,可能是肺癌的潜在病因之一。     

p53、β-连环蛋白与IL-10联合检测对EB病毒相关性胃癌患者的预后评估的临床研究

目的:以EB病毒相关性胃癌患者为研究对象,探究IL-10作为EB病毒型胃癌筛选生物标志物的可能性以及p53和β-连环蛋白水平对患者治疗后预后评估的价值。方法:纳入2014年1月-2015年12月于本院外科接受手术治疗的EB病毒型胃癌患者80例,采集患者血液和少量肿瘤组织;采用Trizol法从新鲜冷冻的肿瘤组织中提取DNA,检测EBV DNA,扩增EBNA-2基因(巢式PCR)和血清学检测抗体水平;采用免疫组织化学法检测肿瘤组织p53蛋白、β-连环蛋白表达及定位情况;采用双抗体夹心ELISA法检测血样中的IL-10水平;采用COX模型进行生存分析。结果:胃癌患者血清抗EB病毒核抗原(EBNA)、IL-10水平均显著高于对照组(t=12.441、11.832,P0.001);p53的阳性表达与低癌细胞分化状态、高T分期均存在显著相关(P0.01),与有淋巴结转移也有密切联系(P0.01);β-连环蛋白的阳性表达与低癌细胞分化状态、淋巴结转移之间有着密切联系(P0.01);在6~24个月的随访观察期间,p53阳性患者、β-连环蛋白阳性患者的生存时间均显著短于阴性患者(P0.05)。结论:p53、β-连环蛋白与IL-10联合检测对EB病毒相关性胃癌的早期诊断和疾病预后评估具有临床运用价值。     

EBN-1和c-erbB-2在乳腺浸润性导管癌的表达

目的 探讨EB病毒(Epstein-Barr virus,EBV)感染与乳腺浸润性导管癌发生发展的相关性及其与癌基因c-erbB-2表达的相关性.方法 应用免疫组化法(immunohistochemmistry,IHC)检测乳腺浸润性导管癌组织、相应癌旁组织及乳腺良性增生组织石蜡标本中EB病毒核蛋白-1(EBNA-1)的表达状况,随后应用相同方法分别检测EBNA-1阳性及阴性石蜡标本癌基因c-erbB-2的表达状况.结果 80例乳腺浸润性导管癌组织中有24例EBNA-1阳性(28.75%),相应癌旁组织及乳腺良性增生组织均为阴性,EBNA-1检出率的差别有统计学意义(χ2=28.235,P<0.001;χ2=6.336,P=0.012).EBNA-1阳性和EBNA-1阴性乳腺癌在年龄、组织病理学分级、临床分期、淋巴结转移的差异均无统计学意义(P值分别为0.868、0.915、0.748、0.812).在24例EBNA-1阳性乳腺癌标本中,均有人类表皮生长因子受体-2(c-erbB-2)表达,阳性率为100%;而在56例EBNA-1阴性乳腺癌标本中仅有25例表达,阳性率为44.6%,二者差异有统计学意义(P<0.01).结论 EB病毒感染对部分乳腺浸润性导管癌的发生发展可能有影响,且可能上调患者癌基因c-erbB-2的表达,但与患者的年龄、组织病理学分级、临床分期和淋巴结转移无关.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.