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1.
目的:标定自制人胎盘凝知酶并应用于临床病人测定。方法:根据WHO参比方法,用已知国际敏感指数的凝血活酶标定自制人胎盘凝血活酶;用进口试剂和自制品测定60例口服华法令抗凝治疗病人和40例肝病患者的PT,求得PTR和INR并进行比较。结果:自制品ISI为1.39,与进口品测定同一标本所得INR比较的回归方程为Y=0.9836X+.06249,两种试剂测定60例抗凝治疗病人所得PT,PTR有明显差异而I  相似文献   

2.
目的:比较三种组织凝血活酶试剂对口服华法令抗凝治疗及肝病患者作凝血酶原时间(PT)测定所得的PT(秒)、PTR、INR间相关性,探讨成都地区用PT监测抗凝治疗的INR参考范围。方法:分别用三种试剂测定60例华法令抗凝治疗病人和40例肝病病人标本的PT(秒),计算出PTP、INR值并作比较。结果:三种试剂测得抗凝治疗病人的PT(秒)、PTR值,进口品与自制猪肺和胎盘绒毛凝血活酶比较有显著性差异。三种试剂测得INR值均无差异。肝病组PT(秒)、PTR、INR均有差别。结论:三种PT试剂均可用于口服抗凝剂治疗的监测,且必须用INR报告结果。成都地区心脏换瓣术后病人口服抗凝剂治疗监测INR参考范围为1.15~3.09。  相似文献   

3.
目的评价东菱克栓酶治疗急性脑梗死的效果。②方法对48例发病3d内的脑梗死病人使用东菱克栓酶进行治疗,并予以临床评分及检测D-二聚体、血液流变学、血小板计数和出、凝血时间。③结果用药后临床症状明显改善(R=0.679,P<0.01);D-二聚体明显升高(t=3.36,P<0.01);血液流变学各项指标均有明显变化(t=4.168~17.774,P<0.01);而血小板计数和出、凝血时间无变化(F=0.418~0.790,P>0.05)。④结论东菱克栓酶对急性脑梗死有较大的治疗价值。  相似文献   

4.
①目的 评价东菱克栓酶治疗急性脑梗死的效果。②方法 对48例发病3d内的脑梗死病人使用东菱克栓酶进行治疗,并予以临床评分及检测D-二聚体、血液流变学、血小板计数和出、凝血时间。③结果 用药后临床症状明显改善(R=0.679,P〈0.01);D-二聚体明显升高(t=3.36,P〈0.01);血液流变学各项指标均有明显变化(t=4.168 ̄17.774,P〈0.01);而血小板计数和出、凝血时间无变化  相似文献   

5.
脂质体包封的肝素喷雾于健康自愿者皮肤上,通过观察凝血参数研究脂质体肝素的透皮吸收能力。结果:15名受试者在给药5000u或者10000u后0.5、1.5h的凝血酶凝固时间(tTC)、激活的部分凝血活酶时间(tAPT)、凝血酶原时间(tP)均比给药前延长,统计学处理差异有显著性(P<0.001~0.05),15例中4名主诉性功能增强,无其他不良反应。提示:本实验所用的脂质体肝素,通过开放式给药,可以穿透皮肤,进入血循环,产生抗凝效果。  相似文献   

6.
目的:观察急性实验性弥散性血管内凝血(DIC)家兔血清NO水平和血管紧张素转换酶(ACE)活性的变化及其关系。方法:用4%兔脑粉(组织凝血活酶)浸液(2ml/kg+生理盐水稀释至30ml)静脉注射复制家兔急性实验性DIC动物模型,用硝酸还原酶比色法测定NO含量,紫外分光光度法测定ACE活性。结果:家兔血清NO在注射浸液开始后15min下降,30、60min升高,与注射前比较均无显著性差异(P>0.05)。15、30min家兔血清ACE活性变化无显著性(P>0.05),但60min明显下降(P<0.01)。DIC组血清NO水平与血清ACE活性呈显著负相关(γ=-0.841,P<0.01)。结论:在组织凝血活酶所致的急性DIC发病机制中,血液NO可能不起重要作用,其变化与血清ACE活性有关  相似文献   

7.
急性脑血管病发病的日节律现象探讨   总被引:1,自引:0,他引:1  
本文对192例共259发病例次的急性脑血管病发病日节律与病灶大小进行了探讨,发现脑血管病在一日24小时中有2个发病高峰,其第1个较大的高峰在5~12时,尤以5~9时、特别5~7时为最高峰,第2个较小的高峰在15~19时,对发病的周期性函数曲线拟合方程是:Y=21.22+9.46sin(0.17πt+303)+8.81sin(0.33πt+238)+l.57sin(0.50πt+58),式中Y为发病频数,t为时间,方程P<0.05,拟合效果良好,还进一步对发病形成的病灶大小进行了多元相关与逐步回归分析,获得脑梗塞病灶大小的方程为:T=63.62+109.70x1-79.97x2+2.15x3,式中T为体积(cm3),x1为血小板聚集率,x2为血浆粘度,x3为发病时间,方程R=0.5034,P<0.2,这些结果均显示了在脑血管病发病中时间因素的重要性。  相似文献   

8.
目的:比较三种组织凝血活酶试剂对口服华法令抗凝治疗及肝病患者作凝血酶原时间测定所得的PT,PTR,INR间相关性,探讨成都地区用PT监测抗凝治疗的INR参考范围。方法:分别用三种试剂测定60例华法令抗凝治疗病人和40例肝病病人标本手PT,计算出PTP,INR值并作比较。结果;三种试剂测得抗凝治疗病人的PT,PTR值,进口品与自制猪肺和胎盘绒毛凝血活酶比较有显著性差异。  相似文献   

9.
自1935年Quick创立了凝血酶原时间(PT)测定一期法以来,作为外源性凝血系统的过筛试验和口服抗凝药物治疗的监测指标,PT测定为临床广泛使用。但由于测定PT所采用的主要试剂组织凝血活酶来源、质量等不同,造成PT测定结果差异,从而影响抗凝治疗效果〔...  相似文献   

10.
钒酸盐氧化法测定总胆红素及直接胆红素的实验评价   总被引:2,自引:0,他引:2  
为了解钒酸盐氧化法测定血清胆红素的可行性,进行方法学评价。结果:总胆红素测定平均回收率为100-6 % ,线性达620μmol/L,批内CV% 为2-1% ,批间CV为3-9 % ,与咖啡因法比较,Y=0-991x+ 2.01,t= 2-11,P> 0-2 ,r= 0.998。直接胆红素线性达340μmol/L,批内CV2-3% ,批间CV4-1 % 与改良Doumas 法比较,r= 0.993x+ 0.99,t= 1.47 ,P> 0 .2,r=0 .997。轻度乳糜血,Hb4g/L以下对测定无干扰,试剂至少稳定两个月。结果表明钒酸盐氧化法测定血清胆红素具有灵敏、准确、重复性好等特点,可作为常规法使用。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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