经方小建中汤对老龄鼠胃衰老影响的实验研究

目的观察小建中汤的抗胃衰老作用。方法 45只老龄Wistar大鼠随机分为空白组、模型组、小建中汤组,每组15只。除空白组外其余各组大鼠以10%D-半乳糖腹腔注射(剂量为140mg·kg-1.d-1)造成衰老模型;小建中汤组造模同时予小建中汤灌胃。8周后检测各组大鼠胃黏膜、肝组织SOD活性及MDA含量,摘取大鼠胸腺、脾和胃称重并计算脏器指数。结果给药后小建中汤组脾、胃和胸腺的脏器指数显著增加,与模型组比较,差异有统计学意义(P0.01);小建中汤组胃黏膜、肝组织中SOD活性升高,MDA含量降低,与模型组比较,差异有统计学意义(P0.05)。结论小建中汤具有抗氧化、清除自由基、修复胃黏膜损伤等作用,具有一定的延缓胃衰老作用。     

葡萄籽原花青素对2型糖尿病大鼠认知障碍影响

目的:探讨葡萄籽原花青素(Grape seed proanthocyanidin extracts,GSPE)对2型糖尿病大鼠认知障碍的影响。方法:Wistar大鼠随机分为对照组、实验组。实验组大鼠采用链脲菌素联合高脂饮食复制2型糖尿病大鼠模型。造模成功后,随机分成模型组,二甲双胍组(metformin,MET,140 mg·kg-1·d-1,灌胃),GSPE低、中、高剂量组(GSPE1、GSPE2、GSPE3分别为20、40、80 mg·kg-1·d-1,灌胃)。2个月后,观察各组大鼠Morris水迷宫学习能力、海马丙二醛(MDA)水平及超氧化物歧化酶(SOD)活性。结果:与对照组比较,模型组定位航行和空间探索能力下降(P0.05);MET组、GSPE3组定位航行学习能力和空间探索能力明显高于模型组(P0.05)。与对照组比较,模型组海马SOD活性明显降低(P0.05),MDA含量明显增加(P0.05);与模型组比较,MET组和GSPE3组海马SOD活性显著增加(P0.05),MDA含量显著降低(P0.05)。结论:葡萄籽原花青素能改善2型糖尿病大鼠认知障碍,机制可能与增强SOD活性、清除过多的氧自由基、减少MDA的生成有关,并且葡萄籽原花青素的作用具有剂量依赖性。     

EGCG对2型糖尿病大鼠认知障碍影响

目的:观察表没食子儿茶素没食子酸酯(Epigallocatechin gallate,EGCG)对2型糖尿病大鼠认知功能的影响。方法:雄性Wistar大鼠,除对照组外均采用链脲菌素联合高脂饮食复制2型糖尿病模型。造模成功后,随机分成模型组,二甲双胍组(140 mg·kg-1·d-1,灌胃),EGCG低、中、高剂量组(分别为10、20、40 mg·kg-1·d-1,灌胃)。给药4周后,采用Morris水迷宫法进行行为学检测,测定大鼠空腹血糖(FBG)、血甘油三酯(TG)、总胆固醇(TC)、海马丙二醛(MDA)、一氧化氮(NO)水平及超氧化物歧化酶(SOD)活性。结果:与模型组比较,EGCG高剂量组寻台时间缩短,穿台次数增多(P0.05);FBG、TG、TC、海马MDA和NO含量显著降低,SOD活性显著增加(P0.05)。结论:EGCG可改善2型糖尿病大鼠认知障碍,可能与其降低动物体内血糖、提高抗氧化能力有关。     

阿魏酸钠对D-半乳糖衰老模型小鼠抗氧化活性的研究

目的　探讨阿魏酸钠 (sodiumferulate ,SF)对D -半乳糖诱导衰老模型小鼠抗氧化能力的影响。方法 :D -半乳糖 (15 0mg·kg-1)诱导衰老模型小鼠为实验对象 ,造模同时及造模第 5周始同时ipSF (2 5、 5 0mg·kg-1) ,以体重、免疫器官重量 ,血清、脑SOD ,血清、肝脏MDA ,全血GSH -Px活力和脑MAO含量为指标 ,全面考察SF抗衰老作用。结果 :造模同时注射SF高低剂量组均能显著对抗D -半乳糖所致小鼠血清、肝脏MDA、脑MAO的增加 ,提高血清、脑SOD的活力 ,对抗小鼠体重、胸腺指数下降 ;SF高剂量组显著抑制脾指数降低 ,提高GSH -Px活力。造模第 5周开始同时给药高剂量组显著抑制血清MDA的增加。结论 :SF具有良好的抗氧化能力 ,预防性给药能在一定程度上延缓D -半乳糖引起的小鼠体内的拟衰老变化 ,而治疗性给药抗衰老作用不明显。     

菟丝子多糖抑制衰老小鼠模型中氧自由基域的作用

目的研究菟丝子多糖的抗衰老作用及其机制.方法用D-半乳糖造衰老小鼠模型,同时分别灌胃给予100、200、400 mg·kg-1·d-1菟丝子多糖,观察其对衰老模型小鼠免疫器官及抗氧化系统的影响.结果衰老模型小鼠胸腺指数和脾脏指数下降,组织中脂褐质(lipofuscin,LF)升高,肝、肾组织中丙二醛(malondialdehyde,MDA)升高及超氧化物歧化酶(superoxide dismutase,SOD)及谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)活力下降.分别灌胃给予100、200、400mg·kg-1·d-1菟丝子多糖能使衰老模型小鼠胸腺指数和脾脏指数不同程度升高;肝、肾组织中MDA不同程度下降;SOD及GSH-PX活力不同程度提高;脑组织中LF不同程度下降.结论菟丝子多糖有抗衰老作用,其机制可能与提高免疫功能,清除氧自由基及抗脂质过氧化有关.     

1型糖尿病大鼠模型的建立及糖尿病并发症相关指标的测定

目的 为了研究链脲佐菌素(Streptozotocin,STZ)诱导大鼠1型糖尿病模型的方法并观察糖尿病并发症指标的变化.方法 采用一次性腹腔注射STZ(60mg·kg-1),监测不同时点大鼠的空腹血糖、体重、饮水量及血浆中的丙二醛(maleic dialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)和一氧化氮(nitric oxide,NO)等指标.结果 大鼠注射STZ 60mg·kg-1 72 h后血糖值达到成模标准,并逐渐出现糖尿病表现,观察7周,始终满足成模标准,未见转复.糖尿病组大鼠肾脏及肝脏肥大指数较对照组增加(P相似文献   

大豆异黄酮与葡萄籽原青花素对2型糖尿病大鼠抗氧化功能的影响

目的探讨大豆异黄酮(SIF)与葡萄籽原青花素(GSPC)对2型糖尿病大鼠抗氧化功能的影响。方法30只SD大鼠随机分成普食对照组(A组,6只,喂养基础饲料)和高脂饲料组(24只,高脂饲料喂养)。用链脲佐菌素将高脂饲料组大鼠制备成糖尿病大鼠,将模型制备成功的18只大鼠随机分成3组:糖尿病对照组(B组)、SIF GSPC250mg·kg-1·d-1剂量干预组(C组)和SIF GSPC50mg·kg-1·d-1剂量干预组(D组)。A组和B组大鼠用消毒后的自来水灌胃,C组和D组大鼠以两种剂量的SIF GSPC分别灌胃。检测6周、10周后各组大鼠空腹血糖水平以及10周后大鼠血清超氧化物歧化酶(SOD)活性及还原型谷胱甘肽(GSH)、丙二醛(MDA)、一氧化氮(NO)水平。结果10周后C组和D组大鼠空腹血糖较B组均下降,但只有C组大鼠空腹血糖水平与B组相比,差别均有统计学意义(P<0.05)。C组大鼠血清SOD活性和GSH、MDA、NO水平与B组比较,差别均有统计学意义(P<0.05);D组大鼠SOD活性和NO水平与B组比较,差别有统计学意义(P<0.05)。结论大豆异黄酮协同葡萄籽原青花对于改善2型糖尿病大鼠的抗氧化能力有作用。     

栽培甘草的黄酮提取物对糖尿病大鼠血糖、尿糖及抗氧化作用的影响

目的观察宁夏地区栽培甘草(Glycyrrhiza uralensis Fisch.)提取物甘草黄酮(licorice flavonoids,LF)对2型糖尿病(T2DM)模型大鼠的降糖作用及抗氧化作用的研究。方法采用高脂高糖饮食喂养后(12周)注射链脲佐菌素(streptozotocin,STZ,35mg.kg-1,ip)制备大鼠2型糖尿病模型,实施腹腔注射300mg.kg-1和100mg.kg-1剂量的LF干预40d后,测定空腹血糖、血浆糖化血红蛋白(HbA1C)、尿糖值、24h尿蛋白值和酮体值,以及血清超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)和总抗氧化能力(T-AOC)。结果 LF降低空腹血糖、HbA1C水平、尿糖值,升高血清SOD和GSH-PX水平的同时,降低血清MDA含量。LF对血浆T-AOC、24h尿蛋白值和酮体值,改善不明显。结论宁夏地区栽培甘草中甘草黄酮具有改善实验性2型糖尿病大鼠糖代谢的作用,其抗氧化活性可能与其作用有关。     

人参皂苷、丹参酮和川芎嗪抗小鼠皮肤衰老作用研究

目的:比较人参皂苷、丹参酮和川芎嗪灌服对衰老小鼠皮肤的抗衰老作用.方法:100只雌性昆明种小鼠,随机分为5组(n=20):正常对照组皮下注射生理盐水;其余4组每日颈背部皮下注射D-半乳糖(1 000 mg·kg-1·d-1)造成小鼠衰老模型,同时各组分别灌胃生理盐水(衰老模型组)、丹参酮(1 500 mg·kg-1·d-1)、川芎嗪(150 mg·kg-1·d-1)或人参皂苷(100 mg·kg-1·d-1).42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶(SOD)活力、过氧化脂质代谢产物丙二醛(MDA)含量、过氧化氢酶(CAT)活力和皮肤羟脯氨酸含量.结果:灌服丹参酮和人参皂苷后,衰老模型小鼠皮肤中SOD活力由衰老对照组的(131.2±21.5) U/ml分别提升为(203.1±11.2) U/ml和(196.8±27.5) U/ml,羟脯氨酸含量由(0.57±0.13) mg/g分别提高到(0.71±0.11) mg/g和(0.76±0.12) mg/g,MDA含量由(9.39±1.5) nmol/g降低到(6.4±1.3) nmol/g和(7.16±2.3) nmol/g,差异均有显著性(P<0.05),且丹参酮和人参皂苷的效果相当.而灌服川芎嗪组上述指标的改变不显著.结论:人参皂苷和丹参酮灌服有显著的抗小鼠皮肤衰老作用,均可明显提高皮肤抗氧化酶活力和增强成纤维细胞活性;川芎嗪灌服抗小鼠皮肤衰老作用不明显.     

暖心胶囊对腹主动脉狭窄诱导心衰模型大鼠的影响

[目的]观察暖心胶囊对腹主动脉狭窄诱导心衰模型大鼠的影响.[方法]选用SD大鼠40只,采用腹主动脉狭窄诱导法复制心衰大鼠模型,造模成功大鼠随机分为模型组,暖心胶囊低、高剂量组(剂量分别为0.825、1.650 g·kg-1·d-1),地戈辛组(剂量为0.091 mg·kg-1·d-1),并设假手术组对照;检测各组大鼠心率(Rheart)、外周收缩压(pSB)、外周舒张压(pDB)、左室收缩压(pLVS)、左室舒张末压(pLVED)、左室内压上升/下降最大速率(V±dp/dt)、血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量.[结果]低、高剂量暖心胶囊可升高模型大鼠pSB、pLVS、V±dp/dt,降低pLVED,增加SOD活性,降低MDA含量,与模型组比较有显著性差异(均P＜0.05),并可不同程度减慢心率,但与模型组比较无显著性差异(P＞0.05).[结论]暖心胶囊治疗心衰的作用可能与其能改善心脏收缩与舒张功能,纠正体内自由基代谢紊乱,抑制心肌脂质过氧化有关.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…