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1.
大鼠深Ⅱ度烫伤模型的病理研究   总被引:5,自引:0,他引:5  
封闭群非清洁级和清洁级SD大鼠79只,80℃15s水烫后造成深Ⅱ度烫伤,分别于烫伤后2、4、8、24、48及72h,局部皮肤取材,组织学观察,结果显示表皮细胞及毛囊上皮细胞核固缩,皮脂腺崩解,真皮胶元纤维肿胀融合,深部毛囊残留,确认为深Ⅱ度烫伤,两组动物比较,非清洁级部分大鼠烫伤24h后,烫伤区筋膜内有大量中性白细胞浸润,有的累及肌层,而清洁级大鼠则基本上无炎症细胞浸润。故后作为烫伤模型更为适宜。  相似文献   

2.
目的:研究深Ⅱ°烫伤后早期大鼠空肠P物质(SP)阳性神经变化与血管通透性变化,探讨其相关性。方法:运用免疫组化技术和改良伊文思蓝渗出法。结果:烫伤后1h大鼠空肠SP阳性神经密度达到最高值,后明显下降,烫伤后4h ̄8h达到最低值,随后逐渐恢复;烫伤后1h大鼠空肠血管通透性最高,后缓慢下降,烫伤后8h ̄24h达到最低值,而后略有升高,烫伤后72h接近正常值。深Ⅱ°烫伤后早期大鼠空肠SP阳性神经变化与血  相似文献   

3.
烫伤对大鼠下丘脑视上核内皮素1的影响袁文俊蒋应明向正华△雄性SD大鼠24只,体重280~320g,随机分为烫伤后15min、1,3h及对照组,每组6只。经20%乌拉坦腹腔麻醉(5ml/kg)后,各烫伤组沸水烫背部7s,造成占体表面积20%的Ⅱ度烫伤;...  相似文献   

4.
大鼠浅Ⅱ度烫伤实验模型的病理研究   总被引:1,自引:0,他引:1  
研究高温与烫伤深度之间的关系,热源相同而接触时间不同所致的皮肤浅Ⅱ度烫伤同的发展规律。两组大鼠均以70℃水烫,实验Ⅰ和实验Ⅱ的接触时间分别为15s和10s,两组在烫后8h或6h,创面均属Ⅱ度烫伤,至烫后1d,局部病变加重而累及真皮浅层毛囊,实验组1至烫后2周,除1例创面修复愈合外余4例因创面坏死并发感染而未愈合;而实验组Ⅱ在烫后7d,5例大鼠的创面已基本愈合,说明其热源虽相同,但因接触时间较甜美,  相似文献   

5.
[目的]研究单用虎杖煮液对大鼠深Ⅱ°烫伤的治疗作用。[方法]取大鼠30只,制成深Ⅱ°烫伤模型,随机分成生理盐水对照组、烧烫灵对照组和虎杖煮液组。暴露疗法,无菌技术操作,创面局部擦洗药物。现察药后创面愈合情况,以创面愈合率及时间长短评价药物疗效。[结果]虎杖组第10天、第18天创面愈合率分别是同期生理盐水对照组的3.2倍和2.9倍,25天后虎杖组念合率达97.4%,而生理盐水对照组只有61.6%,虎杖组与各同期烧烫灵对照组无显著差别。[结论]单用虎杖煮液对大鼠深Ⅱ°烫伤有较好的治疗作用。  相似文献   

6.
目的:探索大鼠烫伤后心肌舒缩功能变化及其规律。方法:复制大鼠TBSA30%Ⅲ°烫伤模型,56只随机分成烫伤前及烫伤后 1、3、6、12、24、48及72h 8组,观察各时段左心室力学参数(LVSP、LVEDP、±dp/dtmax)变化。结果:动物烫伤后1h,-dp/dtmax显著下降,LVEDP明显升高; LVSP、+dp/dtmax伤后3h显著下降。最显著的变化是伤后12h,72h仍较明显(P<0.01)。结论:大鼠烫伤后左心室舒缩功能明显下降,且舒张功能障碍的发生早于收缩功能障碍。  相似文献   

7.
小鼠深Ⅱ°烫伤创面愈合过程中成纤维细胞凋亡的意义   总被引:3,自引:0,他引:3  
刘毅  陈璧  贾赤宇  王建波 《医学争鸣》1999,20(5):455-456
目的:了解深Ⅱ°烫伤创面愈合过程中创面成纤维细胞的凋亡及其意义。方法:雄性Balb/c小鼠20只,采用恒温恒压电烫仪于其背部造成直径2.5cm的深Ⅱ°烫伤创面。分别于烫伤前及烫伤后7,14和21d各取5只,拉颈活杀取材。光镜观察计数创面成纤维细胞,利用末端标记技术检测成纤维细胞凋亡数量,同时,利用电镜观察凋亡细胞的形态。结果:创面成纤维细胞数量自伤后7d即较正常对照组明显增加,至伤后14d达到最高  相似文献   

8.
巴曲酶对大鼠深Ⅱ度烫伤创面加深的治疗   总被引:3,自引:0,他引:3  
在深Ⅱ度烫伤8%体表面积的大鼠伤后2,24,48h腹腔注射去纤维蛋白原剂巴曲酶,观察该酶对大鼠深Ⅱ度烫伤创面早期加深是否有缓解作用。结果表明,烫伤大鼠伤后增高的血纤维蛋白原水平降至正常,血和创面内的纤维蛋白(原)降解产物与正常一致或高于正常,创面组织标本Masson胶原染色显示伤后72h内的创面加深现象一定程度的缓解,巴曲酶可通过降解血纤维蛋白原使血液粘稠度降低而改善大鼠深Ⅱ度烫伤创面的微循环损害  相似文献   

9.
烫伤对大鼠下丘脑视上核内皮素1mRNA的影响蒋应明向正华△袁文俊雄性SD大鼠24只,体重280~320g,随机分为烫伤后15min、1,3h及对照组,每组6只。经20%乌拉坦腹腔麻醉(5ml/kg)后,各烫伤组鼠用沸水烫背部7s,造成占体表面积20%...  相似文献   

10.
用黄连、黄柏、黄芩和金银花复方水煎剂治疗烧伤大鼠创面感染,测定血浆中白细胞介素6(IL-6)的含量和混和淋巴细胞培养上清液中的肿瘤坏死因子TNF诱生量。大鼠随机分成假烫组、烧伤创面感染对照组(烧感组)和烧伤创面感染十中药治疗组(中药组)。烧感组和中药组在伤后8、24和48h抽血查上述指标。结果示烧伤创面感染的大鼠自烧伤后24h起血浆IL-6均高于正常,烫伤后48h烧感组血浆IL-6显著高于中药组(P<0.01)。TNF的诱生量从伤后24h起,中药组显著高于烧感组,中药组伤后24和48hTNF的诱生量分别为4.2±0.9ng/ml和2.61±1ng/ml;烧感组分别为1.1±0.3ng/ml和0.9±0.3ng/ml(P<0.01)。由此说明抑制内源性致热因子IL-6和TNF的产生是中药清热解毒的重要机制。  相似文献   

11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

12.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

13.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

14.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

15.
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.  相似文献   

16.
Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.  相似文献   

17.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

18.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

19.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

20.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

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