固相萃取逐步分离果蔬保鲜防腐剂噻苯咪唑、邻苯基苯酚残留检测的研究

目的建立固相萃取逐步分离测定果蔬中残留的噻苯咪唑、邻苯基苯酚的检测方法。方法样品经石油醚∶乙酸乙酯(2∶1)提取,30%甲醇溶液上样,C18固相柱富集、净化、分离2种残留防腐剂,分别采用30%乙醇-酸溶液(pH 2.5)、55%甲醇-碱溶液(pH 11.5)依次洗脱分离固相柱中被保留的噻苯咪唑、邻苯基苯酚,对洗脱液进行紫外分光光度法检测。结果这2种防腐剂在1～10μg/mL的范围内呈良好线性关系,相关系数r>0.999 2。样品加标回收率为83.3%～105.5%,相对标准偏差为1.2%～7.3%,最低检测限分别为0.09、1.0μg/mL。结论本方法适用于果蔬中残留防腐剂的检测。     

骨关节结核与ABO血型的相关性研究

目的探讨骨关节结核与ABO血型间的相关性,为骨关节结核的预防和治疗提供依据。方法选取2011年3月～2016年10月住院的骨关节结核病人433例进行系统的ABO血型分布分析,以同期体检中心5 878名健康体检人群为对照,分析ABO血型与骨关节结核的相关性及相对危险度。结果①健康人群ABO血型分布为:A型25. 5%(1499/5 878)、B型34. 7%(2 042/5 878)、O型29. 9%(1 751/5 878)、AB型10. 0%(586/5 878),各血型分布比例顺序为B> O> A> AB; 433例骨关节结核病人的ABO血型分布为:A型22. 2%(96/433)、B型31. 0%(134/433)、O型34. 9%(151/433)、AB型12. 0%(52/433),各血型分布比例顺序为O> B> A> AB,组间分布差异有统计学意义(P=0. 036 <0. 05);②相对危险度分析:O型血的相对危险度最高,AB型血的相对危险度次之,A型、B型血的相对危险度最低。结论骨关节结核病人中O型血比例及相对危险度均高于健康人群,O型血是骨关节结核的风险因子。     

丙烯酸酯共聚物改性聚氨酯胶粘剂性能的研究

以2402酚醛树脂／聚氨酯为胶粘剂组分，得到具有优秀粘接强度的胶粘剂体系。通过自由基无规共聚合的方法合成出一系列不同玻璃化温度(-40～80℃)的聚丙烯酸酯，并对胶粘剂进行改性。结果表明：2402酚醛树脂的适量加入不仅可以改善胶粘剂对被粘面的浸润性、调节胶粘剂的粘度，还可以提高胶粘剂的粘接性能；使T剥离强度达到45N／cm；而丙烯酸酯共聚物的加入虽然降低了胶粘剂的粘接性能，使T剥离强度降至20～30N／cm，却可拓宽胶粘剂的阻尼温域，达到50℃。用SEM研究了不同组分对胶粘剂微观形貌的影响，用DMA研究了聚丙烯酸酯的改性对胶粘剂的阻尼性能的影响。     

国产BHP6601型SPECT的性能测试与质量控制

通过测试,评估国产BHP6601型SPECT仪的产品质量和各项性能指标.参照NEMA规范和厂方技术标准,以常规测量模型以及相应的放射源(99mTc、131I)对SPECT系统进行全面的性能测量,亦包括系统的物理、机械性能等.固有空间分辨率3.8mm,固有非均匀性±(2.1～3.2)%,固有空间非线性(0.1～0.6)mm,系统空间分辨率(CFOV):FWHM:9.3 mm,FWTM:17.0mm;系统非均匀性:积分±2.3%,微分±1.9%,系统灵敏度(LEGP):12.1s-1(kBq/cm2).结果表明,测量指标达到国家标准.仪器性能稳定可靠,能满足临床需要.     

61例儿童紫癜性肾炎临床病理分析

目的 :通过对 6 1例紫癜性肾炎 ( HSPN)患儿临床病理分析 ,以探讨儿童 HSPN的特点 ,并提高其认识。　方法 :对 HSPN患儿进行临床分型及病理分级。临床上分为 U ab型、I- GH型、R- GH型、NS型和 HT型 ;病理分为 ～ 级。以此对临床表现及病理改变特点进行综合分析 ,阐明其临床与病理之间联系。　结果 :临床分型结果显示 ,6 1例患儿中以 U ab型为最多 ,HT型最少。病理分级中以 级为最多 ;R- GH型和 NS型患儿病理分级在 ～ 级者的比例高于 Uab型和 I- GH型 ;免疫复合物沉积以 Ig A Ig G Ig M同时存在者比例最高 ,R- GH型和 NS型中Ig A Ig G Ig M沉积者的比例高于 U ab型。激素治疗有效组患儿血清白蛋白水平明显高于无效组 ( P<0 .0 5 ) ,无效组患儿蛋白尿水平高于激素治疗有效组。　结论 :儿童 HSPN中临床表现为 NS型和 R- GH型者病理改变相对较重 ,U ab型和 I- GH型患儿病理改变相对较轻 ;免疫复合物沉积为 Ig A Ig G Ig M者病理改变相对较重 ;大部分HSPN患儿激素治疗有效 ,血清白蛋白低者激素疗效较差     

基质辅助激光解吸/电离飞行时间质谱在鼠伤寒沙门菌同源性分析中的应用价值

目的:研究基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)在鼠伤寒沙门菌同源性分析中的应用价值.方法:利用MALDI-TOF MS对分离于广州市的44株鼠伤寒沙门菌进行分型,与脉冲场凝胶电泳分型(PFGE)进行比较,并分析主要质谱型的质谱图.结果:对PFGE结果聚类分析可以将44株鼠伤寒沙门菌分为15型.在聚类相似度为80%时,44株鼠伤寒沙门菌被分为8个MALDI-TOF MS型,与PFGE分型较一致.其中MS1型(52.3%)为主要的质谱型,MS1型包含多个PFGE型.结论:MALDI-TOF MS方法分型结果显示了广州市鼠伤寒沙门菌相对集中的亲缘关系,是一种简便、快速、高通量的方法.     

Chiari I型畸形手术前后的脑干诱发电位研究

目的:研究Chiari I型畸形患者手术前后脑干诱发电位的变化规律,为Chiari I型畸形手术疗效的判定提供客观而准确的依据.方法:对15例Chiari I型畸形患者行环枕减压术、硬膜成形术,手术前第1～3天和手术后第10～14天行脑干诱发电位检测,术后随访6个月,然后进行脊髓功能JOA评分和MRI复查.结果:15例Chiari I型畸形患者手术后临床症状均有所缓解,脊髓功能JOA改善率为48.58%,MRI复查显示脊髓空洞明显缩小,脑干诱发电位检测Ⅰ～Ⅴ峰间期术后比术前缩短(P＜0.05).结论:脑干诱发电位可以作为评价Chiari I型畸形手术前后效果的客观而相对准确的检查方法.     

小儿肺炎支原体肺炎73例X线诊断体会

目的:了解小儿肺炎支原体(MP)肺炎的胸部X线表现,提高对MP肺炎的认识和诊断水平.方法:将73例MP肺炎X线进行分析总结.结果:将X线表现分Ⅰ～Ⅳ类型,Ⅰ型为肺实质的均质的片状阴影,多累及单一肺段,Ⅱ型为肺纹理增多、模糊及弥漫性网状结节状阴影,Ⅲ型为弥漫性淡片状阴影、界限不清,Ⅳ型为肺门淋巴结肿大.结论:MP肺炎以右肺居多(58%),常侵犯中下肺野(85%).Ⅰ型有相对特异性,易伴肺不张或胸腔积液,对诊断MP肺炎帮助极大.Ⅱ～Ⅳ型无特异性.掌握MP肺炎胸片的类型及特点,可提高临床诊治水平.     

中间相沥青用作镁炭砖粘结剂的可行性研究

提出以中间相沥青替代酚醛树脂作镁炭砖粘剂来提高镁炭砖的质量。通过正交实验选定了用作粘结剂的中间相沥青，并探讨了中间相沥青溶剂分级组分对镁炭砖型块耐压强度的影响。两种粘结对比实验表明，中间相沥青结合型块的高温耐压性能优于酚醛树脂结合型块。上述实验结果具有一致性。     

HLA-DQB1基因多态性与2型糖尿病的关系

目的:探讨中国北方汉族人HLA-DQB1等位基因多态性与2型糖尿病的关系.方法:采用PCR技术和限制性内切酶片段长度多态性分析方法对49例2型糖尿病患者及55例正常对照者进行了HLA-DQB1基因分析.结果:病例组和对照组HLA-DQB1基因突变型等位基因(G)频率在病例组和对照组间差异无显著性(χ2=0.36, P>0.05),GG、GC 、CC三种基因型出现频率差异无显著性.基因型频率的相对风险分析结果表明 GC基因型与CC基因型患2型糖尿病风险度为1.02, GG基因型与CC基因型患2型糖尿病风险度为0.43.结论:HLA-DQB1基因突变型等位基因与2型糖尿病发病无必然的联系,突变基因型并没有增加2型糖尿病的发病风险.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…