无排卵月经周期血及腹腔液中β－EP，L－EK，SS的含量变化

本文用放射免疫法测定了有排卵月经周期及无排卵月经周期各２４例妇女的血浆及腹腔液中β－内啡肽（β－ＥＮ，亮氨酸脑啡肽（Ｌ－ＥＫ）和生长抑素（ＳＳ）的含量。结果显示：无排卵月经周期的妇女ＬＨ峰值前后血浆、腹腔液β－ＥＰ含量及腹腔液中Ｌ－ＥＫ含量均显著高于有排卵月经周期妇女，无排卵月经周期中期的β－ＥＰ含量显著升高提示可能参与调控ＬＨ的分泌，抑制排卵前ＬＨ峰，阻断排卵，本文为神经肽类药物以抑制ＬＨ峰，阻断排卵，控制生育，提供一定的理论参数。     

GnRH-a在体外受精—胚胎移植中的应用

目的：探讨促性腺激素释放激素激动剂（GnRH-a)在体外受精－胚胎移植（IVF－ET）中的作用。方法：2001年5月－2002年7月共行IVF－ET39例，对已获成功的20例患者进行回顾性研究。用达菲林或阿拉瑞林进行降调节，其中长方案从治疗周期前一周期的黄体中期开始应用，月经第3天用促性腺激素（Gn)；短方案自治疗周期月经第2天开始应用，第3天开始用促性腺激素（Gn)。结果：长方案组用达菲林全量10例，半量6例，用阿拉瑞林1例；短方案组达菲林1例，阿拉瑞林2例。结论：实施IVF－ET，GnRH-a在控制性超排卵（COH）中具有至关重要的意义，可有效防止过早LH峰、减少取消周期，提高了妊娠率。     

尿蛋白—1与糖尿病肾病关系的研究

目的：为了探讨尿蛋白－1（UP－1）与糖尿病肾病的关系。方法：用酶联免疫法测定了90例非胰岛依赖型糖尿病（NIDDM）患者的UP－1，同时测定尿N－乙酰－β-D－氨基酸糖苷酸（NAG）、β2－微球蛋白（β2-mG)。结果：糖尿病患者UP－1均较正常对照组增高，UP－1与尿A1b、尿NAG和β2-mG呈正相关。结论：在糖尿病早期阶段，肾小管会受到损害，UP－1可作诊断早期糖尿病肾病的敏感指标。     

经前综合征催乳素水平分析（附70例报告）

报告70例经前综合征及18例正常月经周期妇女，用放射免疫法测定其催乳素（PRL），促卵泡素（FSH），促黄体生成素（LH），雌二醇（E2）及孕酮（P）水平。结果表明，两组FSH，E2均值无显著差异（P＞0．05），经前综合征组PRL和LH明显高于正常月经组（P＜0．01）P均值前者显著低于后者（P＜0．01）。用溴隐亭加“经前安”汤剂治疗前综合征对控制症状，巩固疗效，优于单用溴隐亭疗法。     

老年女性心脑血管疾病患者雌激素与血脂测定

目的：研究老年妇性心脑血管疾病患者体内雌激素与血脂的变化。方法；用化学发光法测定68例患才心脑血管疾病（CCVD）的老年女性（病例组）、73例正常老年女性（对照组）的血清雌二醇（E2）、促卵泡刺激素（FSH）和促黄体生成素（LH）；用酶法测定血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL－C）、高密度脂蛋白胆固醇（HDL－C）；用免疫比浊法测定脂蛋白（Lp(a))、载脂蛋白A（apo-A）及载脂蛋白B(apo-B）。结果：病例组与对照组相比，E2、HDL－C和apo-A水平显著降低，FSH、HL、TC、TG、LDL－C和apo-B水平则显著升高（P均＜0.01）。2组间Lp(a)差异无显著性（P＞0.5）。E2与TC、TG、LDL－C及apo-B水平呈显著负相关，相关系数分别为－0.52、－0.41、－0.39和－0.37；E2与HLD－C及apo-A水平呈显著正相关，相关系数为0.39和0.43，P均＜0.01。结论：老年女性雌激素失调与脂代谢异常显著相关，可能是老年女性CCVD发病的主要原因之一。     

原发性育龄不育不孕妇女实验室诊断及实验结果分析

目的：探讨原发性育龄不育不孕妇女的血清生殖激素水平测定的临床价值。方法：对60例原发性育龄不育不孕患者的生殖激素六项FSH（促卵泡成熟激素）、LH（促黄体生成素）、E2（雌二醇）、P（孕酮）、PRL（催乳激素）、T（睾酮）进行检测）。结果：观察组患者不同时期生殖激素6项测定与正常对照组比较变化较大。在卵泡期,观察组中FSH、PRL、E2、P和T指标均表现异常。与对照组比较差异均有统计学意义（P〈0．05）;在排卵期,观察组中FSH、LH、E2和T指标均表现异常,与对照组比较差异均有统计学意义（P〈0．05）;在黄体期,观察组中LH、PRL和P指标均表现异常,与对照组比较均有显著性差异（P〈0．05）。结论：对原发性育龄不育不孕患者进行血清生殖激素水平测定。对病因诊断十分必要,并建议成为诊断不育的常规项目。     

妊娠滋养细胞疾病患者的β-HCG和性激素水平的实验分析

目的：对450例妊娠滋养细胞疾病（gestational trophoblastic disease,GTD)患者的血清和尿液中人绒毛膜促性腺激素β亚单位（β－HCG）及性激素（LH、FSH、E－2、P、PRL、T）的激素水平进行观察，方法：用放射免疫法（RIA）。结果：绝大部分GTD患者尿液中β－HCG的含量比血清中β－HCG的含量高2．5－5倍，GTD患者的血清中E－2、P、PRL的激素水平与正常妊娠妇女相比有明显差别。结论：对GTD应同时检测其血清和尿液中β－HCG的含量，有条件的实验同时检测GTD患者血清中的E－2、P、PRL。     

不孕症妇女超排卵治疗前后免疫学研究

目的 探讨促性腺激素对生殖内分泌－免疫系统的影响。方法 采用全自动发光法、ELISA法测定20例患者超排卵前后卵泡刺激素（FSH）、黄体生成素（LH）、雌二醇（E2）、抗卵巢抗体（AOA）、抗磷脂抗体（PAP）、抗核抗体（ANA）、抗双链DNA抗体（ds-DND)及血清球蛋白IgA,IgG,IgM。结果 治疗后FSH、LH、E2、IgM、IgG较治疗前无显著性变化，IgA明显高于治疗前，治疗后2例患者出现AOA，1例出现ds-DNA。结论 超排卵不影响血清FSH、LH、E2水平，可能对机体的免疫功能有所影响，但无明显临床表现。     

抗卵巢抗体的检测对卵巢早衰的诊断价值

目的：探讨抗卵巢抗体（AoAb）的检测在卵巢早衰（POF）诊断中的临床应用价值。方法：用酶联免疫吸附（ELISA）间接法测定AoAb。结果：POF患血清中AoAb阳性率显高于正常妇女组（P＜0．01）。结论：检测患血清中的AoAb对POF具有重要辅助诊断价值。尤其可应用于血清促卵泡激素（FSH）、黄体生成素（LH）正常的间歇性卵巢早衰的诊断。     

血浆抗苗勒管激素/苗勒管抑制物浓度预测辅助生育技术结局的价值高于促卵泡激素、抑制剂B及雌二醇

目的:对假说“卵泡早期抗苗勒管激素(A M H)及苗勒管抑制物(M IS)的血浆浓度是卵巢应答及辅助生育技术(A RT)结局的有效标记物进行验证。方法:研究对象为109例年龄在42岁以下行体外受精(IV F)诱导排卵的患者,私人A RT中心对其治疗前月经周期第3天的血样标本进行回顾性分析研究。使用促卵泡激素(FSH)刺激卵泡发育后进行IVF卵泡抽吸术,对使用FSH无效的月经周期使用促性腺激素释放激素激动剂(G nR H-a)治疗。结果:分析月经周期第3天血浆A M H/M IS、雌二醇μg/L(E2)、FSH及抑制剂B的浓度与IVF结局(如获取的成熟卵母细胞数目、…     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…