阿霉素肾病大鼠肾组织中核因子—kB活化与肾小管间质损害的关系

目的：探讨阿霉素肾病大鼠肾组织中核因子－kB（NF－kB）活化及其与蛋白尿、肾小管间质损害和趋化因子表达之间的关系。方法：阿霉素肾病模型采用尾静脉注射阿霉素（6mg/kg）制备。应用免疫组化和图像分析系统观察肾皮区小管间质损害程度、NF－kB活化及单核细胞趋化蛋白－1（MCP－1）表达。结果：阿霉素肾病大鼠出现大量蛋白尿及明显的皮质区肾小管间质损害。肾皮质区NF－kB活化及MCP－1表达显著增强,且与蛋白尿及肾小管间质损害程度显著相关。结论：NF－kB活化介导出非免疫性慢性肾小球炎时皮质区肾小管间质损害及诱导趋化因子的产生。     

白三烯受体拮抗剂对阿霉素肾病大鼠肾间质炎症的影响

目的 探讨白三烯受体拮抗剂对肾小管间质炎症损伤的影响.方法 采用阿霉素肾病大鼠模型,2周成模后用高、低两个剂量的白三烯受体拮抗剂--孟鲁司特(Montelukast)进行治疗,用泼尼松治疗作为对照.HE染色观察肾脏病理;免疫组化检测细胞间黏附分子-1(ICAM-1)和单核细胞趋化因子-1(MCP-1)在肾小管间质中的表达;24h尿蛋白测定采用考马亮蓝方法.结果 模型组大鼠肾脏病理.肾小管上皮浊肿、严重的空泡变件;肾小管不同程度扩张,可见较多蛋白管型;肾间质小灶性一灶性炎细胞浸润.白三烯受体拮抗剂高、低剂量治疗均可显著减少肾小管间质炎细胞浸润,小管上皮浊肿、变性等程度减轻,蛋白管型也较模型组减少.模型组大鼠肾小管问质ICAM-1、MCP-1的表达较正常大鼠显著增强,白三烯受体拮抗剂和激素治疗可显著降低ICAM-1、MCP-1的表达.结论 白三烯受体拮抗剂可显著改善阿霉素肾病大鼠肾小管间质病理损伤和炎细胞浸润,其机制可能通过其抑制白三烯作用而减少了ICAM-1、MCP-1等炎症因子有关.     

肾毒血清性肾炎大鼠肾组织中核因子-кB活化及单核细胞趋化蛋白-1表达

目的:探讨核因子-κB(NF-κB)活化在肾毒血清性肾炎发病机制中的作用。方法:肾毒血清肾炎应用兔抗鼠肾小球基底膜肾毒血清制备。应用凝胶电泳迁移率检测肾组织中NF-κB活化;采用免疫组化及彩色病理图文分析系统观察肾小球及肾小管中单核细胞趋化蛋白-1(MCP-1)表达,并分析其与蛋白尿和肾小球细胞数之间的关系。结果:模型组肾组织中NF-κB活化较正常对照组显著上调;肾小球及肾小管中MCP-1表达分别为(24.37±7.06)个/肾小球横切面(gcs)和(54.78±11.49)%,较正常对照组显著升高(P<0.01),肾组织中NF-κB活化和MCP-1表达与单核细胞浸润和蛋白尿密切相关。结论:NF-κB活化在肾小球肾炎发病机制中发挥重要作用。     

六味地黄汤对5/6肾切除大鼠NF-κB、MCP-1、Col-Ⅲ表达的影响

目的 观察六味地黄汤对5/6肾切除大鼠残肾转录因子-核因子κB (NF-κB)、单核细胞趋化因子(MCP-1)及Ⅲ型胶原(Col-Ⅲ)表达的影响,探讨六味地黄汤抑制肾间质纤维化进展的作用机制.方法 按照随机数字表法将60只SD雄性大鼠分为5组:空白组、假手术组、模型组、依那普利组、六味地黄汤组,每组12只,后三组行5/6肾切除术诱导大鼠肾衰模型,假手术组同期手术,但不损及肾脏.、造模术后3d进行灌胃干预.灌胃8周后处死各组大鼠,观察各组大鼠残肾组织形态学变化并用免疫组化法检测大鼠残肾NF-κB 、MCP-1 、Col-Ⅲ的表达.结果 (1)观察5/6肾切除大鼠残肾组织细胞形态学,与模型组相比六味地黄汤、依那普利组可减轻大鼠肾间质损害及纤维化程度;(2)免疫组化半定量分析显示六味地黄汤组肾皮质的NF-κB、MCP-1及Col-Ⅲ表达均明显低于模型组(P＜0.05),与依那普利组差异无统计学意义(P＞0.05).结论 六味地黄汤可能通过下调NF-κB 、MCP-1及Col-Ⅲ的表达,减少细胞外基质的积聚,减轻炎症反应和纤维化程度,抑制5/6肾切除大鼠肾间质纤维化进展,可作为慢性肾衰竭的辅助用药、     

糖尿病肾病患者单核细胞趋化蛋白-1与小管间质损害关系的研究

目的探讨局部单核细胞趋化蛋白-1(MCP-1)表达和分泌与人糖尿病肾病(DN)病变的关系.方法采用免疫组化和原位杂交检测人DN肾组织MCP-1蛋白和mRNA表达,并用免疫组化检测CD68;尿液和血清MCP-1水平测定采用ELISA法.结果DN肾小管间质MCP-1表达明显增高(P＜0.01),小管间质病变越重,MCP-1表达越高(P＜0.01).DN肾小管间质CD68表达明显增高(P＜0.01),小管间质病变间差异明显(P＜0.01).DN肾小球MCP-1、CD68表达较正常对照明显增高(P＜0.05).DN尿液MCP-1水平明显较正常对照组增高(P＜0.01),与间质纤维化密切相关,且与临床蛋白尿程度呈正相关.结论DN患者肾组织内MCP-1表达与肾病变发生发展密切相关,尤其与肾小管间质病变的形成有关,这可能与MCP-1具有强烈趋化和激活单核/巨噬细胞而致肾小管间质损害有关,尿液MCP-1水平可能是反映肾小管间质病变程度的最佳指标之一.     

原发性肾病综合症患者肾组织及尿液MCP-1的表达及意义

【目的】：探讨原发性肾病综合症患者(PNS)肾组织及尿液单核细胞趋化因子(MCP-1)表达水平及其在肾小管间质损伤中的意义。【方法】用免疫组化法检测56例PNS患者肾组织MCP-1的蛋白表达，ELISA法检测治疗前及治疗后3个月时尿液MCP-1质量浓度。【结果】正常肾组织无MCP-1表达，肾病患者均有不同程度表达，表达部位主要在肾小管上皮细胞与肾间质，肾组织及尿液MCP-1表达水平均显著高于对照组。强的松或强的松 环磷酰胺治疗3个月后尿液MCP-1表达水平显著降低。微小病变患者肾组织及尿液MCP-1表达水平显著低于其它病理类型患者，膜增殖性性肾炎患者显著高于其它类型患者。治疗后未完全缓解患者尿液与肾小管间质MCP-1表达水平显著高于完全缓解患者。肾组织及尿液MCP-1表达水平与肾脏病理类型、肾小管间质病变程度及血清肌酐水平、肌酐清除率显著相关。【结论】MCP-1在PNS患者肾小管间质病变发生机制中起重要作用，检测肾组织及尿液MCP-1表达水平对PNS患者病情、治疗反应、预后判断及病理诊断有一定价值。     

原发性肾病综合症患者肾组织及尿液MCP-1的表达及意义

[目的]探讨原发性肾病综合症患者(PNS)肾组织及尿液单核细胞趋化因子(MCP-1)表达水平及其在肾小管间质损伤中的意义.[方法]用免疫组化法检测56例PNS患者肾组织MCP-1的蛋白表达,ELISA法检测治疗前及治疗后3个月时尿液MCP-1质量浓度.[结果]正常肾组织无MCP-1表达,肾病患者均有不同程度表达,表达部位主要在肾小管上皮细胞与肾间质,肾组织及尿液MCP-1表达水平均显著高于对照组.强的松或强的松+环磷酰胺治疗3个月后尿液MCP-1表达水平显著降低.微小病变患者肾组织及尿液MCP-1表达水平显著低于其它病理类型患者,膜增殖性性肾炎患者显著高于其它类型患者.治疗后未完全缓解患者尿液与肾小管间质MCP-1表达水平显著高于完全缓解患者.肾组织及尿液MCP-1表达水平与肾脏病理类型、肾小管间质病变程度及血清肌酐水平、肌酐清除率显著相关.[结论]MCP-1在PNS患者肾小管间质病变发生机制中起重要作用,检测肾组织及尿液MCP-1表达水平对PNS患者病情、治疗反应、预后判断及病理诊断有一定价值.     

厄贝沙坦对AngⅡ介导的肾小球系膜细胞核因子-κB活化及MCP-1表达的影响

目的 观察不同剂量厄贝沙坦(Irb)对血管紧张素Ⅱ(Ang Ⅱ)介导的肾小球系膜细胞(GMC)核因子-κB(NF-κB)活化及单核细胞趋化因子-1(MCP-1)表达的影响,探讨Irb抑制GMC炎症因子表达的可能机制.方法 分离培养大鼠GMC,用10-6mol/L Ang Ⅱ刺激后将其随机分为5组(n=4):对照组、Ang Ⅱ刺激组、Ang Ⅱ和3种不同浓度(10-5mol/L、10-6mol/L、10-7mol/L)的Irb共孵育组.分别采用ELISA法及半定量RT-PCR法检测细胞培养上清液中MCP-1、I κB、NF-κB的表达水平和GMC内MCP-1 mRNA的表达水平,并在酶联免疫激光扫描共聚焦显微镜下观察GMC内NF-κB p65核易位情况,计算核易位阳性率.结果 与Ang Ⅱ刺激组比较,其他各组MCP-1、I κB、NF-κB水平及MCP-1 mRNA表达均明显较低(P<0.05或0.01);与低浓度Irb组比较.高浓度Irb组MCP-1、IκB、NF-κB、MCP-1 mRNA表达及中浓度Irb组MCP-1水平均明显较低(均P<0.05);与中浓度Irb组比较,高浓度Irb组MCP-1、IκB、NF-κB水平及MCP-1mRNA表达均明显较低(均P<0.05).结论 Irb可能是通过抑制NF-κ B的活化,降低GMC MCP-1的表达以减少肾小球系膜区单核细胞的浸润,从而减轻肾小球炎症反应.     

辛伐他汀对大鼠肾小管上皮细胞转分化的作用

目的:观察辛伐他汀对实验性1型糖尿病大鼠肾小管上皮细胞转分化的作用。方法:将实验动物随机分为对照组、糖尿病组及治疗组。治疗组给予辛伐他汀[5 mg/(kg.d)]治疗12周。于实验第6,9,12周检测24 h尿蛋白(24 h TP),12周检测血清肌酐(Scr),内生肌酐清除率(Ccr)和NAG酶。采用免疫组织化学技术检测各组肾小管间质中-αSMA,MCP-1,CD44表达水平。结果:糖尿病大鼠肾组织中-αSMA,MCP-1和CD44表达较对照组明显增加(P<0.01),治疗12周后,辛伐他汀可明显降低1型糖尿病肾病大鼠肾小管间质中-αSMA,MCP-1和CD44表达(P<0.01),并降低蛋白尿(P<0.01),改善肾功能各指标及肾组织病理学损害。-αSMA表达分别与MCP-1,CD44表达及24 hTP呈正相关(r=0.998 5,0.976 3,1.000 0,P<0.01)。结论:辛伐他汀对糖尿病肾小管间质病变具有保护作用,其机制至少部分与抑制肾小管上皮细胞转分化,下调肾小管间质中-αSMA,MCP-1和CD44表达有关。     

阿霉素肾病大鼠肾小管MMP-9, TIMP-1的免疫组织化学观察

目的:观察基质金属蛋白酶-9(matrix metalloproteinase-9, MMP-9)及组织金属蛋白酶抑制剂-1(tissue inhibitor of metalloproteinase-1, TIMP-1)在阿霉素肾病大鼠肾小管中的表达变化. 方法:采用大鼠尾静脉单剂量注射阿霉素7.5 mg/kg建立阿霉素肾病模型,免疫组织化学检测正常对照组、阿霉素肾病组肾组织MMP-9及TIMP-1的表达变化;光镜下观察肾小管间质形态学改变;尿液标本检测尿N -乙酰-βD氨基葡萄糖苷酶(N-acetyl-beta-D-glucosaminidase, NAG),β2-微球蛋白(β2-microglobulin, β2-MG), 24 h尿蛋白量的变化. 结果:阿霉素肾病组大鼠肾小管间质损害明显,24 h尿蛋白定量增多,尿β2-MG及尿NAG酶活力升高;肾小管上皮细胞MMP-9的表达明显上调(P<0.05),于第14日达高峰. 肾小管的TIMP-1的表达上调,第14, 28, 56日与同期对照组相比差异显著(P<0.05). 结论:阿霉素肾病大鼠肾小管上皮细胞MMP-9, TIMP-1表达异常可能参与肾小管病变的发生发展过程.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.