伤寒杆菌内毒素诱导的SD大鼠全葡萄膜炎

[目的]用伤寒杆菌内毒素在SD大鼠建立葡萄膜炎模型.[方法]将200μg伤寒杆菌内毒素注射于SD大鼠双足底部,观察眼部临床改变和组织学改变,并测定房水中蛋白浓度.[结果]内毒素注射4h后出现炎症,以后炎症加重,表现为瞳孔缩小、前房明显闪辉、细胞和渗出,甚至出现后房积脓,于24h发现房水蛋白浓度显著增高,虹膜睫状体、视网膜以及脉络膜有大量的白细胞及一定量的单核细胞和淋巴细胞浸润.[结论]伤寒杆菌内毒素在SD大鼠诱导出严重的葡萄膜炎,它可作为人类全葡萄膜炎的动物模型.     

伤寒杆菌内毒素诱导的SD大鼠全葡萄膜炎

目的：用伤寒杆菌内毒素在SD大鼠建立葡萄膜炎模型。方法：将200μg伤寒杆菌内毒素注射于SD大鼠双足底部，观察眼部临床改变和组织学改变，并测定房水中蛋白浓度。结果：内毒素注射4h后出现炎症，以后炎症加重，表现为瞳孔缩小、前房明显闪辉、细胞和渗出， 甚至出现后房积脓，于24h发现房水蛋白浓度显增高，虹膜睫状体、视网膜以及脉络膜有大量的白细胞及一定量的单核细胞和淋巴细胞浸润。结论：伤寒杆菌内毒素在SD大鼠诱导出严重的葡萄膜炎，它可作为人类全葡萄膜炎的动物模型。     

氢饱和生理盐水对内毒素诱导的大鼠葡萄膜炎的影响

目的 探讨氢饱和生理盐水(hydrogen rich saline,HRS)对内毒素诱导的大鼠葡萄膜炎(endotoxin-induced uveitis,EIU)是否有减轻作用.方法 健康成年雄性SD大鼠按随机数字表法分为正常组、模型组、氢饱和生理盐水(氢水)组和激素组,于后3组大鼠足垫部皮下注射0.125 mg/kg内毒素诱导葡萄膜炎.氢饱和生理盐水组大鼠于造模后0、0.5、1、2、6、8、12、24 h和3周内每天1次,腹腔注射氢饱和生理盐水20 mL/kg;激素组大鼠于造模后立即腹腔注射地塞米松磷酸钠注射液1 mg/kg.4组于造模后12、24、48、72、96 h在裂隙灯下观察葡萄膜炎表现,并进行量化评分;并于造模后1、4、7、10、14 d和21 d行视网膜电图检查、房水蛋白定量及病理组织学检查.结果 氢饱和生理盐水组葡萄膜炎程度与模型组相似,裂隙灯临床表现量化评分和炎症细胞计数差异均无统计学意义(P＞0.05);内毒素诱导的大鼠葡萄膜炎模型的视网膜功能有改变,暗适应3.0反应b潜伏期延长;与模型组相比,氢饱和生理盐水组b波潜伏期有缩短趋势,但差异无统计学意义(P＞0.05);氢饱和生理盐水组房水蛋白总量较模型组略低,差异有统计学意义(P＜0.05).结论 氢饱和生理盐水对内毒素诱导的大鼠葡萄膜炎无明显减轻作用.     

特异性免疫核糖核酸对内毒素诱导的实验性葡萄膜炎的抑制作用

目的：探讨特异性免疫核糖核酸（iRNA）对内毒素诱导的大鼠葡萄膜炎（EIU）是否有抑制作用。方法：用大肠杆菌（E．Coli)免疫家兔后,提取特异性iRNA,将其用于大肠杆菌内毒素诱导的大鼠葡萄膜炎模型,通过生化检测、细胞计数评价其对实验性葡萄膜炎的影响。结果：在内毒素注射后30min及3h,分别经腹腔注射iRNA,可以使EIU的发生推迟3h,炎症的严惩程度明显下降,尤其表现为炎症高峰期,前房内白细胞 出量下降27．62％,蛋白渗出量减少58．34％。结论：特异性iRNA对内毒素诱导的大鼠葡萄膜炎有明显的抑制作用。     

重组人Slit2蛋白预处理可减轻内毒素诱导的大鼠葡萄膜炎

目的 探讨重组人Slit2蛋白(recombinant human slit2,rhSlit2)对内毒素诱导的Sprague-Dawley(SD)大鼠葡萄膜炎(endotoxin-induced uveitis,EIU)的作用.方法 给予SD大鼠玻璃体腔内分别注射rhSlit2(10、30、100 ng/眼).24 h后,将2 g/L的脂多糖(lipopolysaccharides,LPS)注射到大鼠双侧后足底肉垫内(200 μg/鼠).LPS注射24 h后观察大鼠前房炎症并进行临床评分;取大鼠房水进行房水蛋白浓度测定和房水细胞计数;LPS注射24 h后摘除大鼠眼球,制作石蜡切片HE染色后观察大鼠眼部形态学变化;采用Real-time PCR检测大鼠眼部炎症因子白介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)mRNA水平的表达;Western blot检测大鼠眼部IL-6、TNF-α和PI3 K/Akt信号通路蛋白的表达.结果 在LPS注射24 h后,rhSlit2对前房炎症有明显的抑制作用,且具有剂量依赖性.LPS+rhSlit2(100 ng/眼)组临床评分明显低于LPS+PBS组[(1.40 ±0.84)vs(5.60 ±0.97)分,P＜0.01];HE染色可见各组大鼠眼内结构层次完好.与LPS+PBS组比较,LPS+rhSlit2(100 ng/眼)组眼内炎症细胞渗出数量明显减少[(65.21±18.38)×106/mL vs(319.60±28.88)×106/mL,P＜0.01];房水蛋白浓度明显降低[(12.59 ±3.04)vs(31.50 ±3.09) mg/mL,P＜0.01];眼内炎症因子IL-6(19.69±5.28 vs 77.09±12.61)、TNF-α(1.78±0.33 vs4.06±0.58)的mRNA表达降低(P＜0.01);大鼠眼内IL-6(1.92 ±0.60 vs 4.42 ±0.11)、TNF-α(1.33 ±0.11 vs 1.69±0.21)和P-Akt(0.18 ±0.37 vs 0.55 ±0.34)蛋白水平也降低(P＜0.01).结论 大鼠玻璃体腔RhSlit2预注射对内毒素诱导的SD大鼠葡萄膜炎具有抑制作用.RhSlit2可通过抑制PI3K/Akt信号通路的活化以抑制LPS诱导炎症反应.     

5-磷酸腺苷诱导低温对大鼠内毒素血症的影响

目的探讨5-磷酸腺苷(5′-AMP)诱导的低温对大鼠内毒素血症的影响。方法选用健康雄性Wistar大鼠60只,随机分为空白对照组、脂多糖(LPS)炎症组、5′-AMP低温治疗组、5′-AMP低温预防组。LPS炎症组采用腹腔注射LPS来制备内毒素血症模型;5′-AMP低温治疗组是在LPS腹腔给药后,大鼠体温升高0.5℃时给予5′-AMP进行低温干预;5′-AMP低温预防组是先注射5′-AMP制备低温动物模型,然后给予LPS进行炎症诱导。应用ELISA法测定LPS注射后3、6和12h大鼠血清中炎症因子的浓度,用苏木精-伊红染色法对大鼠肺组织进行病理分析。结果与LPS炎症组比较,5′-AMP诱导的低温能明显降低血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β和IL-6水平,显著提高血清中抗炎症因子IL-10水平,差异有统计学意义(F=2.50～26.73,q=6.36～214.85,P<0.05)。病理分析显示,5′-AMP诱导的低温能明显抑制LPS诱导的急性肺损伤。结论 5′-AMP诱导的低温对LPS所致大鼠内毒素血症和急性肺损伤有显著的治疗和预防作用。     

前房相关免疫偏离对内毒素诱发的大鼠眼葡萄膜炎的影响

目的　探讨前房相关免疫偏离 (ACAID)形成前后对内毒素诱发的葡萄膜炎 (EIU)的影响及机制。方法　将 5 μl光感受器间维生素A类结合蛋白 (IRBP) ( 10 μg/ μl)注射至SD大鼠前房诱导ACAID的动物模型 ,于前房注射IRBP后 3d( 3d组 ,n =18)、7d( 7d组 ,n =18)行足底注射 2 0 0 μg伤寒杆菌内毒素 (LPS)。同时进行迟发性超敏反应 (DTH)的检测以监测ACAID的形成。酶联免疫吸附分析 (ELISA)法检测血清白细胞介素 10 (IL 10 )水平 ,并取脾组织行逆转录聚合酶链反应 (RT PCR)和Western免疫印迹 ,检测GATA 3基因及蛋白表达的变化 ,进行半定量分析。LPS注射后 2 4h观察EIU的临床表现 ,并摘取眼球行组织学检查。LPS单纯注射组作对照组 (n =2 4 )。结果对照组和 3d组DTH呈阳性反应 ,血清中未测出IL 10 ,GATA 3基因和蛋白表达无增加 ;7d组DTH呈阴性反应 ,血清中IL 10升高 ( 7 1pg/ml± 1 3pg/ml,P <0 0 1) ,GATA 3基因和蛋白表达升高。临床检查可发现 ,对照组和 3d组于LPS注射后 2 4h眼部炎症达高峰 ,瞳孔中央完全被纤维素样渗出膜遮盖 ,7d组大鼠眼部充血、渗出明显减轻 ;组织学检查发现 7d组较对照组和 3d组虹膜及睫状体基质内、前房、后房和玻璃体内细胞渗出明显减少。结论　前房注射抗原 7d后 ,GATA 3及IL 10的表     

实验性葡萄膜炎大鼠眼部IL-23mRNA及IL-17mRNA的表达

目的 探讨白细胞介素(IL)-23、IL-17在实验性内毒素诱导葡萄膜炎(EIU)动物模型眼部表达的特点.方法 SD大鼠随机分为两组:正常对照组、EIU模型组.伤寒内毒素致敏诱导大鼠建立EIU模型,于建模后6、12、24、48、60 h用裂隙灯显微镜进行眼前节检查,并根据Hoekzema方法进行眼部炎症评分,RT-PC...     

汉防己甲素对大肠杆菌脂多糖所致急性肺损伤大鼠炎症介质的影响

目的 探讨中药单体汉防己甲素(trandrine,Tet)干预对静脉注射内毒素(LPS)所致急性肺损伤(ALI)的影响及其可能机制.方法 将健康雄性Wistar大鼠32只,随机分为4组:即正常组、模型组、Tet预防组和Tet治疗组.除正常组外,其他3组采用大肠杆菌LPS静脉注射造成急性肺损伤模型,Tet预防组和Tet治疗组分别于注射LPS前后30 min内缓慢注射T注射液(20 mg/kg),正常组给予等量生理盐水.于初始(0)、0.5、2,4、6 h 5个时间点采集动脉血测定动脉血氧分压(PaO2)和动脉血二氧化碳分压(PaCO2).实验结束时测定肺组织湿干重比(W/D),检测支气管肺泡灌洗液(BALF)白细胞总数及总蛋白含量;ELISA法测定外周血及BALF中炎症介质TNF-α及BALF中IL-1β、IL-6浓度;测定肺组织一氧化氮(NO)浓度.结论 模型组在LPS输注完毕后PaO2逐渐下降,PaCO2逐渐下降(P<0.05),Tet预防组0.5、2、4、6 h,Tet治疗组2、4、6 h PaO2高于模型组(P<0.05),PaCO2与模型组差异无统计学意义(P<0.05);模型组肺组织W/D值和NO含量、BALF中自细胞计数、总蛋白含量、IL-1β、TNF-α、IL-6浓度显著性增高(P<0.05),两T组均能减轻上述变化(P<0.05).结论 Tet早期干预能减轻内毒素所致急性肺损伤的炎症介质失衡.     

血必净对内毒素休克大鼠心肌TNF-α变化的影响

目的:本实验通过建立大鼠内毒素休克模型,旨在探讨血必净注射液对休克大鼠心肌细胞肿瘤坏死因子(TNF-α)变化的影响,为临床脓毒症休克的复苏治疗提供相关依据.方法:本研究选用Wistar成年雄性大鼠65只,随机分为四组,即正常对照组(5只)、内毒素组(15只)、治疗组1(15只)和治疗组2(15只);采用腹腔注射大肠杆菌脂多糖(LPS)方法制备大鼠内毒素休克模型,除正常对照组外,其余三组均腹腔注射LPS15mg/kg,注射完毕后即刻内毒素组大鼠予以皮下注射生理盐水10ml/kg抗休克治疗,而治疗组1和治疗组2则分别皮下注射血必净注射液5ml/kg和10ml/kg,正常对照组大鼠经腹腔注入等量生理盐水.除正常对照组外其余四组大鼠按不同时相(腹腔注射后30min、2h、6h)分为3个亚组,在不同时相点留取股静脉血标本以及心室肌标本,通过ELISA法测定TNF-α值.结果:内毒素组大鼠静脉血标本于30min时间点出现TNF-α值高峰,2h和6h时有所下降,其中30min的TNF-α值与正常组及对照组相比存在明显差异(P<0.01).结论:1.内毒素休克可导致心肌细胞炎症信号通路的激活,导致TNF-α的分泌高峰出现.2.炎症信号通路的激活可以加重心肌本身的自身免疫性炎症损伤.3.血必净注射液可以抑制TNF-α的分泌和表达,减轻心脏本身的炎症反应.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.