雷公藤内酯醇对牛晶状体上皮细胞增殖的抑制及其胞浆内cGMP浓度的影响

目的:探讨雷公藤内酯醇(Triptolide, Tri)抑制体外培养的牛晶状体上皮细胞(bovine lens epithelial cell, BLECs)增殖及其细胞内cGMP浓度变化的机制.方法:取第4代BLECs,分3组培养.空白对照组:以DMEM培养;增殖对照组:以DMEM 50 μg/L rhEGF培养;雷公藤内酯醇组:增殖对照组 20 μg/L雷公藤内酯醇.用MTT比色法检测增殖抑制率来测定Tri 20 μg/L对细胞增殖的影响,用放射免疫法测定BLECs内cGMP浓度.结果:浓度为20 μg/L的Tri、作用在处于增殖状态的BLECs时间分别为6,12,24,48,72 h,均出现明显的增殖抑制作用, 其增殖抑制率升高呈现明显的时间依赖性.而雷公藤内酯醇组细胞内cGMP含量比增殖对照组明显降低.结论:Tri对晶状体上皮细胞增殖有明显的抑制作用,而该作用可能通过降低细胞内cGMP浓度来完成.     

雷公藤内酯醇对牛晶状体上皮细胞增殖及胞浆内cAMP浓度的影响

目的:探讨雷公藤内酯醇(triptolide, Tri)抑制体外培养的牛晶状体上皮细胞(bovine lens epithelial cell, BLECs)增殖及其机制.方法:取第4代BLECs,用MTT比色法检测增殖抑制率来测定Tri 20 μg/L对细胞增殖的影响,用放射免疫法测定BLECs内cAMP浓度.结果:Tri 20 μg/L,时间6,12,24,48,72 h作用在处于增殖状态BLECs,出现明显的增殖抑制作用, 其增殖抑制率升高呈现明显的时间依赖性.而增殖抑制组细胞内cAMP含量明显增加.结论:Tri对晶状体上皮细胞增殖有明显的抑制作用,而该作用可能通过影响细胞内cAMP等第二信使物质浓度来完成.     

DIM对胃癌细胞SGC-7901体外增殖与侵袭的抑制作用

目的 探讨3,3-二吲哚基甲烷(3,3-Diindolylmethane,DIM)对胃癌细胞SGC-7901增殖和侵袭的抑制作用.方法 用MTT法检测不同浓度(0、10、20、40、80 μmol/L) DIM对胃癌细胞SGC-7901生长抑制情况;Transwell小室检测40 μmol/L的DIM对胃癌SGC-7901细胞侵袭力的影响,Western blot检测胃癌细胞中血小板源性生长因子-D(PDGF-D)和β-catenin蛋白表达的变化,ELISA法检测培养液上清中MMP-2、MMP-9的浓度.结果 不同浓度的DIM对胃癌细胞SGC-7901均有抑制作用(P＜0.05),随着药物浓度增加,抑制作用增强;但40 μmol/L和80 μmol/L差异无统计学意义(P＞0.05).40μmol/L的DIM作用24 h后胃癌细胞SGC-7901侵袭力下降75.2％(125.4 ±10.8 vs 31.1 ±4.5,P＜0.05),PDGF-D和β-catenin的表达分别下降了55.8％和52.5％(P＜0.05),培养液上清中的MMP-2、MMP-9的浓度明显下降(23.5 ±2.6 vs 13.2 ±1.9;30.4 ±2.4 vs 15.8±2.1,P＜0.05).结论 DIM对胃癌细胞的增殖和侵袭有抑制作用,其机制可能与PDGF-D、β-catenin、MMP-2、MMP-9的表达下降有关.     

牙骨质附着蛋白对牙周膜细胞黏附、伸展及增殖的影响

目的 观察牙骨质附着蛋白(CAP)对体外培养的人牙周膜细胞(hPDLCs)黏附、伸展和增殖活性的影响.方法 组织块培养法获得人hPDLCs.培养液中分别加入不同浓度的CAP(0、0.125、0.25、0.5、1.0、2.0 μg/mL).细胞计数法检测不同浓度CAP对hPDLCs黏附的影响;同时通过计数检测视野中伸展的细胞数,计算hPDLCs在培养1、4、7 h 后的伸展率;MTT比色法测定、比较不同浓度CAP处理组hPDLCs细胞的增殖活性.结果 0.5、1.0 μg/mL CAP处理组hPDLCs的黏附力明显强于空白对照组(CAP浓度为0)(P＜0.01);不同浓度CAP处理组与空白对照组对细胞伸展影响的比较无明显差异.CAP对hPDLCs的促增殖作用呈浓度和时间依赖性,0.5～2.0 μg/mL的CAP均能显著促进hPDLCs的增殖(P＜0.05).结论 CAP对体外培养的hPDLCs具有促黏附及促增殖作用,但对其细胞伸展无显著影响.     

蛇葡萄素对肾癌786-O细胞增殖和凋亡的作用

目的 探讨蛇葡萄素(AMP)对人肾癌786-O细胞株增殖与凋亡的作用.方法 将不同浓度的AMP作用于体外培养的肾癌786-O细胞株,采用MTT法检测不同浓度的AMP对肾癌786-O细胞增殖的作用的影响;采用Annexin V/PI双染法检测不同浓度的AMP对肾癌786-O细胞凋亡的作用.结果 MTT结果显示AMP能显著抑制肾癌786-O细胞增殖,半数抑制浓度(IC50)为17.23 μg/mL;经不同浓度的AMP作用7h后,肾癌786-O细胞凋亡率明显增加,AMP浓度为10 μg/mL和20 μg/mL作用于肾癌786-O细胞后,细胞凋亡率升高(P＜0.05);而AMP浓度为40μg/mL和80 μg/mL作用细胞后,细胞凋亡率显著升高(P＜0.01).结论 AMP在体外能显著抑制肾癌786-O细胞增殖,且能促进肾癌786-O细胞凋亡.     

全反式维甲酸对恶性黑素瘤A375细胞增殖及Fas蛋白表达的影响

目的 观察全反式维甲酸(ATRA)对体外培养恶性黑素瘤A375细胞增殖的抑制作用并探讨其作用机制.方法 设置溶剂对照及ATRA不同浓度组,采用四甲基偶氮唑蓝(MTT)法检测ATRA不同浓度、作用不同时间各组细胞存活和生长情况,分析量效、时效关系;Western blot检测10 μmol/L ATRA作用不同时间Fas蛋白的表达.结果 ATRA在0.01～0.1 μmol/L浓度对A375细胞增殖抑制作用不明显;在1～100 μmol/L浓度有抑制作用;药物作用72 h时各浓度组的细胞抑制率均达到高峰,以100 μmol/L浓度组抑制率最高;10 μmol/L ATRA可上调A375细胞Fas蛋白的表达.结论 ATRA对A375细胞增殖的抑制作用呈时间和剂量依赖关系.ATRA可能通过Fas信号转导途径诱导A375细胞凋亡.     

rhEGF对人皮肤成纤维细胞的促增殖作用

目的 研究重组人表皮生长因子(rhEGF)在不同浓度下对人皮肤成纤维细胞增殖的影响,为临床准确定量应用这两种因子修复创面、加速创面愈合提供理论依据. 方法 体外培养人皮肤成纤维细胞,分别用浓度为0,10,30,50,80,100,150 μg/L的rhEGF干预细胞,48 h后四甲基偶氮唑盐比色法(MTT)检测细胞的活性.选择最适浓度生长因子干预的细胞,用流式细胞仪检测细胞的DNA倍体情况. 结果 不同浓度的rhEGF分别对成纤维细胞干预48 h后,显示rhEGF在80μg/L浓度时MTT值最大;并检测出在最适浓度生长因子干预24 h后细胞大多处于DNA合成前期和合成期. 结论 rhEGF浓度在30-100μg/L之间对人皮肤成纤维细胞有明显的促增殖作用,其中以80μg/L较显著.     

槲皮素对宫颈癌细胞C33A增殖和凋亡的影响

目的 观察槲皮素对人宫颈癌细胞C33A增殖和凋亡的影响,初步探讨其相关作用机制.方法 用不同浓度槲皮素(空白对照、20、40、80 μmol/L),顺铂(空白对照、5、10、15、20 μg/mL)以及两者联合(槲皮素40 μmol/L+顺铂10 μg/mL)分别作用于C33A细胞24 h和48 h后,噻唑蓝(MTT法)检测细胞活力的变化;流式细胞术检测槲皮素对C33A细胞周期的影响;Western blot检测槲皮素对C33A细胞Cyclin D1、Caspase-3和Caspase-9蛋白表达的影响.结果 MTT结果显示,经过槲皮素处理24 h后,各组细胞活力分别为86.92 ±3.953)%(20 μmol/L组)、(66.54 ±3.932)%(40 μmol/L组)和(52.21 ±2.970)%(80 μmol/L组),均低于空白对照组的(98.35 ±1.230)%;经过槲皮素处理48 h后,各组细胞活力分别为(65.19 ±7.071)%(20 μmol/L组)、(47.04 ±8.881)%(40 μmol/L组)和(29.71 ±6.505)%(80 μmol/L组),均低于空白对照组的(96.97 ±1.788)%.;此外,槲皮素40 μmol/L+顺铂10 μg/mL联合作用于C33A细胞24h后,细胞活力下降为(31.12 ±2.835)%;48 h后,细胞活力下降为(17.86 ± 3.182)%,同空白对照组相比均出现了明显的下降,(31.12 ±2.835)%;48 h后,细胞活力下降为(17.86 ±3.182)%(P<0.05).细胞周期检测结果显示,槲皮素可增加C33A细胞G0/G1期数量,减少S期数量.Western blot 结果显示,槲皮素可下调C33A细胞Cyclin D1蛋白的表达,还可上调Caspase-3和Caspase-9蛋白的表达.结论 槲皮素通过下调Cyclin D1蛋白的表达可以抑制C33A细胞的活力和增殖,槲皮素通过上调Caspase-3和Caspase-9蛋白的表达,可以诱导C33A细胞的凋亡.     

Inhibitory Effect of Quercetin on Activity of SW1353 Cells

目的 研究槲皮素对人软骨肉瘤细胞(SW1353)增殖的抑制作用.方法 用体外细胞培养技术与显微镜观察50、100、200、400 μmol/L浓度的槲皮素溶液作用于SW1353细胞,MTT法检测细胞生长抑制率.结果 加药48 h后,随着槲皮素浓度的增高,对SW1353增殖的抑制作用增强,具有显著的浓度依赖性.结论 槲皮素可促进人软骨肉瘤细胞凋亡,具有抗肿瘤作用.     

大黄素对人结肠癌Lovo细胞增殖周期影响的实验研究

目的 观察大黄素对人结肠癌Lovo细胞增殖及周期的影响.方法 采用MTT法检测大黄素不同浓度梯度(20、40、80 μmol/L)和时间梯度(12、24、48 h)处理后Lovo细胞的增殖活力;采用流式细胞仪检测Lovo细胞周期变化.结果 大黄素对Lovo细胞的增殖抑制作用呈明显的浓度和时间依赖性,流式细胞仪分析发现在大黄素作用下Lovo细胞被阻滞于G0/G1期,阻止细胞进入S期,并成剂量依赖性.结论大黄素对结肠癌Lovo细胞增殖具有显著的抑制作用;且使Lovo细胞增殖周期停滞于G0/G1期,而阻止其进入S期,具有良好的结肠癌临床应用前景.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

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