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1.
《延边医学院学报》2015,(3):174-177
[目的]观察桦褐孔菌多糖对脂多糖(LPS)刺激小鼠巨噬细胞株Raw264.7细胞与小鼠腹腔巨噬细胞促炎性细胞因子表达的影响.[方法]利用桦褐孔菌多糖(40mg/L)处理被LPS(100μg/L)刺激的Raw264.7细胞株和小鼠腹腔巨噬细胞,采用半定量RT-PCR方法测定促炎性细胞因子肿瘤坏死因子(TNF)-α和白细胞介素(IL)-1βmRNA的表达;采用ELISA法测定TNF-α和IL-1β水平.[结果]与给予LPS刺激相比较,给予桦褐孔菌多糖和LPS同时刺激的Raw264.7细胞株和小鼠腹腔巨噬细胞促炎性细胞因子TNF-α和IL-1βmRNA表达水平均明显降低(P<0.05);给予桦褐孔菌多糖和LPS同时刺激的Raw264.7细胞株在48 h后与只给予LPS刺激相比较,细胞所分泌的促炎性细胞因子TNF-α和IL-1β水平均明显下降(P<0.05).[结论]桦褐孔菌多糖对LPS刺激Raw264.7细胞株和小鼠腹腔巨噬细胞促炎性细胞因子TNF-α和IL-1βmRNA表达水平及Raw264.7细胞株促炎性细胞因子TNF-α和IL-1β的分泌均有抑制作用.  相似文献   

2.
【目的】观察黄芩苷对脂多糖(LPS)诱导小鼠巨噬细胞产生抵抗素和白细胞介素-1β(IL-1β)的影响。【方法】体外培养小鼠巨噬细胞RAW 264.7,取生长良好的细胞进行接种。模型组加入LPS(终浓度为0.1μg/mL或5μg/mL)或重组抵抗素(终浓度为100 ng/mL);中药组加入不同浓度黄芩苷(终浓度为50、100μmol/L),预处理1 h,后加入LPS或抵抗素。24 h后吸取上清,采用酶联免疫吸附(ELISA)法检测各组IL-1β和抵抗素浓度,提取细胞总RNA,采用实时荧光定量PCR法检测抵抗素mRNA表达。【结果】与正常组比较,LPS组促进了巨噬细胞分泌抵抗素,抵抗素mRNA表达上调,LPS和重组抵抗素均促进了巨噬细胞产生IL-1β(P<0.05或P<0.01)。黄芩苷高、低剂量组均可显著抑制IL-1β和抵抗素的浓度,且抑制抵抗素mRNA表达(P<0.05或P<0.01)。【结论】黄芩苷可明显降低促炎因子产生的炎症反应,这可能是其抗动脉粥样硬化的机制之一。  相似文献   

3.
【目的】观察四氢黄连碱(THC)的体外抗炎作用及其相关机理。【方法】将体外培养的小鼠腹腔巨噬细胞分为空白对照组,模型对照组,四氢黄连碱低、中、高剂量组。以脂多糖(LPS)刺激小鼠原代腹腔巨噬细胞建立体外细胞炎症模型,作为模型对照组。四氢黄连碱低、中、高剂量组分别以不同浓度(终浓度为1、2、3μmol/L)的四氢黄连碱预培养小鼠巨噬细胞24 h后,加入LPS(终浓度为10μg/mL)。空白对照组加入相同体积的细胞培养液。继续培养12 h后,采用硝酸还原酶法检测细胞上清液中一氧化氮(NO)的含量;实时定量PCR(q PCR)法检测细胞内肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)mRNA的表达;酶联免疫吸附分析(ELISA)检测细胞上清液中TNF-α、IL-6含量;ELISA检测细胞内核因子kappa B(NF-κB)与DNA的结合活性。【结果】与空白对照组比较,模型对照组小鼠巨噬细胞上清液中NO、TNF-α、IL-6含量,巨噬细胞TNF-α、IL-6 mRNA表达及NF-κBp65与DNA的结合活性均显著升高(P 0.001);经四氢黄连碱预处理小鼠巨噬细胞24 h后,上述指标均显著降低,且呈剂量依赖性(P 0.05或P 0.01或P 0.001)。【结论】四氢黄连碱具有显著的体外抗炎作用,其机理可能与阻断细胞内NF-κB信号通路的激活,从而下调促炎因子NO、TNF-α及IL-6的表达和分泌有关。  相似文献   

4.
[目的] 通过构建脂多糖(LPS)诱导的RAW264.7巨噬细胞炎症模型,探讨清感冬饮(QGDY)的抗炎作用及其机制。[方法] 采用CCK-8法和LDH法检测不同浓度的清感冬饮对HEK293T细胞和RAW264.7巨噬细胞的细胞活力及LDH漏出量的影响。分别建立抗氧化反应元件(ARE)和核因子-κB(NF-κB)双荧光素酶报告系统,考察清感冬饮对HEK293T细胞ARE、NF-κB表达情况的影响。建立LPS诱导的RAW264.7巨噬细胞体外炎症模型,将RAW264.7巨噬细胞随机分为空白对照组、LPS模型组、清感冬饮低、中、高剂量组(1、10、100μg/mL),倒置显微镜下观察各组细胞的形态差异;采用Griess法和ELISA法测定各组细胞上清中一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)的含量;采用qRT-PCR法检测各组细胞中TNF-α、IL-6和IL-1β的mRNA表达水平;利用免疫荧光法观察核因子-κBp65(p65)核移位情况;采用Westernblot法检测各组细胞一氧化氮合酶(iNOS)和p65蛋白表达情况。[结果] 0.01~10μg/mL清感冬饮对HEK293T细胞活力无显著影响,1~10μg/mL清感冬饮明显抑制TNF-α诱导的NF-κB启动子的活性,清感冬饮对ARE启动子的活性无显著影响。初步证明清感冬饮具有抗炎作用,而无明显抗氧化作用。0.01~100μg/mL清感冬饮干预24h对RAW264.7巨噬细胞无细胞毒性作用。与模型组相比,1~100μg/mL清感冬饮显著抑制LPS诱导的RAW264.7巨噬细胞中NO及炎症因子TNF-α、IL-6和IL-1β的释放;10~100μg/mL清感冬饮显著下调TNF-α、IL-6和IL-1β的mRNA表达水平,抑制p65核移位;100μg/mL清感冬饮显著抑制总蛋白iNOS、核蛋白p65表达,显著促进浆蛋白p65表达。[结论] 清感冬饮可抑制LPS诱导的RAW264.7巨噬细胞的炎症反应,该抗炎作用可能与调控NF-κB/iNOS/NO信号通路、抑制促炎因子释放有关。  相似文献   

5.
目的:观察半夏泻心汤体外抑制巨噬细胞分泌炎症因子的作用及对MAPK炎症信号通路的影响,以探讨其治疗胃炎的可能机制。方法:取小鼠8只,提取小鼠腹腔巨噬细胞,原代培养;用家兔制备半夏泻心汤含药血清和对照血清。小鼠腹腔巨噬细胞分为空白对照组、脂多糖(LPS)组、地塞米松组及半夏泻心汤5%含药血清组、10%含药血清组和20%含药血清组,各组分别给予对照血清、LPS、地塞米松和各浓度含药血清。作用6 h后收集细胞,用qRT-PCR方法检测细胞内IL-1β、IL-8和TNF-α的mRNA相对表达量;作用24 h后,用ELISA方法检测细胞培养上清中炎症因子IL-1β、IL-8和TNF-α的蛋白含量。收集20%含药血清作用30 min、60 min和2 h后的细胞,用Western blot方法检测细胞外调节蛋白激酶(ERK)和P38的磷酸化水平。结果:qRTPCR结果显示,与不含药血清对照组比较,LPS能明显促进小鼠腹腔巨噬细胞IL-1β、IL-8和TNF-α的mRNA表达(P0.01);与LPS组比较,地塞米松能明显抑制小鼠腹腔巨噬细胞IL-1β、IL-8和TNF-α的mRNA表达(P0.01);5%含药血清能明显抑制巨噬细胞TNF-α的mRNA表达(P0.01),10%和20%含药血清均能明显抑制IL-1β、IL-8和TNF-α的mRNA表达(P0.01)。ELISA结果显示,5%含药血清能明显抑制小鼠腹腔巨噬细胞IL-1β的分泌(P0.01),10%、20%含药血清能明显抑制小鼠腹腔巨噬细胞IL-1β、IL-8和TNF-α的分泌(P0.05,P0.01)。Western blot结果显示,半夏泻心汤含药血清作用30 min、60 min均能抑制小鼠腹腔巨噬细胞MAPK信号通路中P38磷酸化水平(P0.05、P0.01);含药血清作用2 h能抑制巨噬细胞的ERK和P38的磷酸化水平(P0.01)。结论:半夏泻心汤可能通过调节巨噬细胞内MAPK炎症信号通路中ERK、P38的磷酸化水平,抑制巨噬细胞分泌炎症因子IL-1β、IL-8和TNF-α而起到治疗胃炎的作用。  相似文献   

6.
目的 通过构建脓毒症肺损伤体内及体外模型,探讨外源性白细胞介素-4(IL-4)可否减轻脓毒症急性肺损伤及其可能的调节机制。方法 细胞实验:体外培养小鼠肺泡巨噬细胞MH-S,选用终浓度为1.0μg/mL的脂多糖(LPS)刺激2 h后,加入不同浓度的IL-4作用24 h, CCK-8法检测细胞活力;活性氧(ROS)测定试剂盒检测ROS的含量,Elish法检测IL-1β、IL-6、TNF-α表达水平,Western blot法检测NF-κB p65蛋白表达水平。小鼠实验:选用C57BL/6小鼠,随机分为空白对照组(生理盐水)、模型组(LPS)及实验组(LPS造模30 min后气管滴注IL-4),12 h后,将3组小鼠经异氟醚诱导后处死收集血清和组织样本。使用H&E染色法观察肺损伤,采用qRT-PCR和ELISA法检测相关基因的表达,流式细胞术检测巨噬细胞的极化情况。结果 外源性IL-4可明显改善肺损伤。体外实验表明,外源性IL-4在24 h内对MH-S细胞的活力几乎无影响;外源性IL-4能够降低MH-S细胞中TNF-α、IL-6、IL-1β水平,下调NF-κB p65蛋白表达量以及减...  相似文献   

7.
目的 探讨小白菊内酯(PTN)对炎症模型小鼠的巨噬细胞抗氧化能力的影响.方法 对15只8周Balb/c小鼠灌服大肠杆菌脂多糖构建炎症模型动物,对成功获取的15只炎症模型动物进行腹腔冲击收集巨噬细胞进行编号和体外共培养,然后体外培养巨噬细胞,随机分为对照组、A组、B组、C组、D组,每组3只,分别对应为空白对照、7.5μmol/L PTN+1 mg/L LPS、15μmol/L PTN+1 mg/L LPS、30μmol/L PTN+1 mg/L LPS及200μmol/L维生素C+1 mg/L LPS.上述各组细胞在相同的培养条件下实施体外共培养试验,在培养第24、48及72小时收集细胞上清液进行炎性因子(IL-1β、TNF-α、IL-6)酶联免疫吸附试验检测,同时对细胞抗氧化能力指标(SOD、MDA、GSH-Px)水平进行检测.结果 炎症模型动物的体外分离获得的巨噬细胞墨汁吞噬染色的吞噬率为95.5%,碱性磷酸酶染色阳性率为91.5%;在与PNT共培养24 h,C组TNF-α均显著高于其他各组(P<0.05);共培养24、48 h,C组IL-1β显著低于其他各组(P<0.05),C组GSH-Px水平显著高于其他各组(P<0.05),C组MDA水平显著低于其他各组(P<0.05);共培养72 h,对照组IL-1β、TNF-α水平均显著高于其他各组(P<0.05),且对照组SOD水平均显著低于其他各组(P<0.05),C组TNF-α、MDA均显著低于其他各组(P<0.05),且C组GSH-Px水平均显著高于其他各组(P<0.05).各组在与PNT共培养第24、48及72小时期间的IL-6水平比较差异均无统计学意义.结论 小白菊内脂可提升巨噬细胞的抗氧化活力,抑制炎性因子分泌,以30μmol/L PTN+1 mg/L LPS进行体外抗氧化效果最佳.  相似文献   

8.
目的研究胸腺素β4(thymosin β4,Tβ4)对内毒素休克小鼠72 h存活率的影响,并初步探讨Tβ4治疗内毒素休克的机制.方法选用清洁级昆明小鼠腹腔注射脂多糖(LPS)制备内毒素休克动物模型,(1)观察内毒素休克前后Tβ4变化情况;(2)观察注射LPS后不同时间给予Tβ4对内毒素休克小鼠存活率的影响;(3)观察不同剂量Tβ4对内毒素休克小鼠存活率的影响;(4)ELISA法检测Tβ4对内毒素休克小鼠血浆TNF-α及IL-1β变化情况的影响;(5)用RT-PCR的方法检测外周血单核细胞(PBMC)的TNF-α mRNA及IL-1β mRNA表达情况.结果 Tβ4在内毒素休克小鼠的血浆中明显降低,A、B、C组内毒素休克小鼠72 h存活率均明显低于D组(P<0.01,P<0.05),Tβ4预处理LPS组TNF-α及IL-1β血浆水平明显低于LPS组(P<0.01),LPS组TNF-α mRNA及IL-1α mRNA的表达明显高于Tβ4预处理LPS组(P<0.01).结论 Tβ4治疗内毒素休克应在LPS腹腔注射后0 h、2 h、4 h连续注射Tβ4(5 mg/kg),Tβ4可降低PBMC表达TNF-α mRNA及IL-1β mRNA.实验提示Tβ4可能是通过下调炎症介质的基因表达来治疗内毒素休克的.  相似文献   

9.
目的:从炎症理论角度出发,探讨薏苡仁多糖(Coixan)对溃疡性结肠炎(ulcerative colitis,UC)体外炎症模型的干预作用及其机制。方法:体外培养NCM460细胞,采用不同剂量(0、2.5、5、10、20和40μg/mL)脂多糖(lipopolysaccharide,LPS)诱导NCM460细胞,筛选出最佳诱导剂量(20μg/mL);用不同浓度(0、5、10、20、40和80μg/mL)的Coixan诱导NCM460,筛选出最佳促增殖浓度(10、20、40μg/mL)。实验随机设为空白组、模型组(LPS诱导)、LPS+Coixan低、中、高剂量(10、20、40μmol/L)组,LPS+柳氮磺吡啶(sulfasalazine,SASP)(200μg/mL)组,除空白组外用LPS(20μg/mL)刺激48 h建立UC体外细胞炎症模型后,分别以薏苡仁多糖及SASP进行48 h干预治疗后,ELISA法检测细胞上清液中炎症因子白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平;免疫荧光...  相似文献   

10.
初步探讨1,3-二环己基-1,2,3,6-四氢嘧啶-4,5-二甲酸二乙酯(ZL-5010)体内、体外的抗炎镇痛活性。方法 醋酸所致小鼠扭体反应模型评价镇痛作用;二甲苯致小鼠耳廓肿胀模型和角叉菜胶致大鼠足跖肿胀模型评价抗炎作用。细菌脂多糖(LPS,10 μg/mL)刺激小鼠腹腔渗出细胞活化作为体外炎症模型。ELISA法检测白介素-1β (IL-1β)和肿瘤坏死因子-α (TNF-α)的含量。结果 ZL-5010经灌胃给药,每天1次,连续3 d,在0.25和0.5 0 mmol/kg的剂量下能减少醋酸所致小鼠的扭体次数,抑制二甲苯所致的小鼠耳廓肿胀,抑制角叉菜胶诱导的大鼠足跖肿胀,均具有统计学意义(P<0.05);ZL-5010体外能抑制LPS诱导的小鼠腹腔渗出细胞产生促炎细胞因子IL-1β和TNF-α,具有统计学意义的最低有效浓度分别为10和20 μmol/L(P<0.05)。结论 首次发现ZL-5010经动物体内灌胃给药具有抗炎镇痛作用,体外可抑制小鼠腹腔渗出细胞产生促炎细胞因子IL-1β和TNF-α。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

20.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

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