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1.
抗肝纤维化姜黄素脂质体的制备及初步稳定性考察   总被引:3,自引:0,他引:3  
目的:制备姜黄素(Curcumin,Cur)脂质体并考察其包封率及稳定性.方法:采用逆相蒸发法制备Cur脂质体,葡聚糖凝胶柱层析法分离含药脂质体与游离药物并测定包封率;正交设计优选制备处方;离心加速实验与渗漏率的测定考察脂质体的稳定性.结果:所得优化处方为磷脂与药物的重量比60:1,磷脂与胆固醇的重量比4:1,pH6.5的磷酸盐缓冲液水合递质,超声时间为5 min.以优化处方制得的脂质体平均包封率达95.06%.结论:选用逆相蒸发法优化Cur脂质体处方合理,工艺简便可行,包封率高,稳定性好.  相似文献   

2.
目的 对雷公藤有效部位脂质体的处方进行优化。方法 运用薄膜分散技术与微孔滤膜法进行脂质体的制备。使用紫外分光光度法对雷公藤有效成分的主要代表建立标准曲线。通过正交试验法,以脂质体包封率为考察指标,对胆固醇和磷脂的比例、药物和磷脂的比例、超声时间在3水平下进行筛选,优化雷公藤有效部位脂质体的处方工艺。结果 雷公藤有效部位脂质体的最佳处方工艺是胆固醇∶磷脂为1∶25;药物:磷脂为1:20;超声时间为4 min。按最佳处方制备的脂质体平均粒径为(55.57±29.43)nm,Zeta电位为0.349 m V,平均包封率达到89.29%。透射电镜下观察制备的雷公藤有效部位脂质体呈类球形。结论雷公藤有效部位脂质体的最佳处方工艺稳定可靠,验证结果重复性佳。  相似文献   

3.
目的:研究川芎嗪脂质体的制剂。方法:以乙醚注入法制备川芎嗪脂质体,以正交设计优化制备工艺,并对其包封率进行计算。结果:最佳工艺条件为药物∶磷脂为1∶10,PBS缓冲液pH为7.0,胆固醇∶磷脂为1∶3,乙醚∶PBS缓冲液为1∶6。结论:川芎嗪脂质体制剂包封率高、稳定性好,适合开发应用。  相似文献   

4.
目的:研究复方鸦胆子油脂质体的处方筛选及制备工艺。方法:采用气相色谱法测定脂质体包封率。以脂质体包封率为主要指标,对制备工艺中可能影响脂质体性质的工艺参数和处方因素进行单因素考察和正交设计,优化制备工艺。用不同冻干保护剂进行冷冻干燥,以筛选合适的保护剂。测定Zeta电位、粒径大小及分布。结果:采用乙醇注入法制备复方鸦胆子油脂质体;最佳制备工艺为磷脂浓度为1.5%,磷脂胆固醇质量比为5∶1,油水两相体积比为1∶10,甘露醇∶蔗糖∶磷脂质量比为1∶1∶1,水相介质为蒸馏水,温度为50℃,搅拌速度为30 rpm。最佳处方制备的平均包封率为91.9%,Zeta电位为-32.1 mV,平均粒径为170.3 nm。结论:该实验制备的复方鸦胆子油脂质体具有包分率高、稳定性好、表面带负电等特点,为进一步研究复方鸦胆子油脂质体奠定了基础。  相似文献   

5.
目的 用离子抑制反相色谱法测定克林霉素磷酸酯片中克林霉素的含量。方法 采用C18柱 ,流动相为磷酸二氢钾(KH2 PO4 0 1mol·L-1)—乙腈 (775∶2 2 5 ) ,流速 1 0ml·min-1,柱温为室温 ,进样量 2 0 μl,检测波长 2 10nm ,工作曲线法定量。结果 方法的精密度用平均RSD表示为 1 6% ,测定克林霉素线性范围为 0 0 4~ 1 0 0 g·L-1,平均回收率为 (99 5±1 5 5 ) %。结论 该法简单、快速、准确。可用于克林霉素磷酸酯片中克林霉素的含量测定  相似文献   

6.
目的 研究古糖酯脂质体的制备方法,并建立其包封率的测定方法.方法 采用反相蒸发法制备古糖酯脂质体,运用正交实验确定最适的制备条件;采用高效凝胶渗透色谱法(HPGPC)测定古糖酯脂质体的包封率.结果 采用反相蒸发法制备古糖酯脂质体的最适条件是:磷脂与胆固醇的摩尔比为4:1,古糖酯药物的加入量为1%,脂水相体积比为3:1,在此条件下制备的脂质体包封率可达39.13%.经负染色法观察古糖酯脂质体的形态为圆形或椭圆形,平均粒径为200 nm.采用HPG-PC法可有效地分离古糖酯脂质体与游离药物,方法测定的线性范围为0.2~10.0 g·L-1,平均回收率为95.12%,RSD为1.83%(n=5).结论 反相蒸发法制备的古糖酯脂质体包封率高,粒径小,形态稳定.HPGPC方法简便、快捷,重现性好,适合于古糖酯脂质体包封率的测定.  相似文献   

7.
目的:制备包封率高、粒径均匀、稳定性好的pshRNA-survivin脂质体,并对其质量进行评价.方法:采用逆相蒸发法制备pshRNA-survivin脂质体;正交设计优选制备处方;考察脂质体的粒径分布、zeta电位;用超滤离心法测定脂质体的包封率;离心加速实验与渗漏率的测定考察脂质体的稳定性.结果:所得优化处方为磷脂...  相似文献   

8.
阿奇霉素脂质体的制备及其包封率测定   总被引:11,自引:0,他引:11  
目的:制备阿奇霉素脂质体并测定其包封率.方法:采用薄膜蒸发-冻融法制备阿奇霉素脂质体,以透析-高效液相色谱法测定含量和包封率.采用Lichrosphere-C18反相色谱柱(150 mm×4.6 mm,5 μm),以乙腈-异丙醇-0.002 mol/L磷酸氢二钾(60∶25∶15,v/v/v)为流动相,流速1.2 ml/min,检测波长215 nm,进样量20 μl.结果:阿奇霉素脂质体的平均体积粒径大于7 μm,包封率大于75%.选定的色谱条件下,阿奇霉素与空白辅料完全分离,在5.08~508 μg/ml范围内,药物浓度与峰面积呈良好的线性关系,r=0.9999.结论:薄膜蒸发-冻融法适用于制备肺靶向阿奇霉素脂质体;透析-高效液相色谱法操作简单,准确,重复性好,可用于测定阿奇霉素脂质体的含量和包封率.  相似文献   

9.
盐酸万古霉素阳离子脂质体的制备及其性质研究   总被引:1,自引:0,他引:1  
目的制备万古霉素阳离子脂质体(cationic liposomal vancomycin,CLVs),确定其最佳处方制备工艺,并研究其性质。方法采用正交法确定逆相蒸发法制备CLVs的最佳处方;采用HPLC法测定药物含量;用微型葡聚糖凝胶柱分离脂质体混悬液中的CLVs与游离药物;在电镜下观察CLVs的形态,并采用激光散射法测定其粒径和Zeta电位。结果制备的CLVs为多囊脂质体;通过正交实验,确定逆相蒸发法制备CLVs的最佳处方为∶磷脂∶硬脂酰胺∶胆固醇的摩尔比为7∶3∶1,脂质∶药物的质量比为15∶1,有机相∶水相的体积比为3∶1;制备的脂质体平均粒径、Zeta电位、pH值以及包封率分别为(185.75±16.33)nm、(69.11±4.62)mV、(6.98±0.01)和(8.58±0.045)%,脂质体在4℃和-80℃条件下保存3个月,稳定性良好,药物泄漏率〈5.0%。结论采用逆相蒸发法制备万古霉素阳离子脂质体,方法简便,重现性好,包封率较高。  相似文献   

10.
PEG修饰大蒜素长循环脂质体的制备工艺研究   总被引:1,自引:0,他引:1  
目的:制备PEG修饰大蒜素长循环脂质体。方法:合成聚乙二醇单甲醚磷脂酰乙醇胺衍生物(PEG-PE),采用Szoka改进逆相蒸发技术,制备PEG修饰大蒜素长循环脂质体(PEG-DATS-LCL),均匀设计法筛选优化处方工艺。结果:根据优化工艺制得的长循环脂质体粒子分布较均匀。测得脂质体包封率为90.77%,算术平均粒径为12.63μm,粒径分布在5~30μm范围内占总数目的91.8%。结论:该制备工艺包封率高,稳定性好,粒径大小符合肺靶向要求。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

20.
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.  相似文献   

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