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1.
观察了经雷公藤单体T_4处理2h后对正常人和类风湿关节炎(RA)患者外周血单个核细胞(PBMC)及RA患者消化的滑膜单个细胞(RA-DSSC)分泌TNF的影响。在35ng/ml浓度下,正常人PBMC(n=10)及RA-PBMC(n=6)T_4处理组产生TNF活性水平明显低于对照组(P<0.001)。延长T_4作用时间至6h,RA-PBMCT_4处理组TNF活性水平下降更为明显(P<0.001),表明T_4可抑制PBMC体外产生TNF活性水平,其对RA-PBMC作用不同时间的抑制率分别为25.54%及40.51%(P<0.01),说明T_4抑制作用可能与作用时间有关。同时T_4亦可抑制RA-DSSC体外产生TNF活性水平(P<0.01),提示T_4有望成为一新的植物性治疗RA药物。  相似文献   

2.
观察了经雷公藤单体T4处理2h后对正常人和类风湿关节炎患者外周血单个核细胞及RA患者消化的滑膜单个细胞分泌TNF的影响。在35ng/ml浓度下,正常人PBMC(n=10)及RA-PBMC(n=6)T4处理组产生TNF活性水平明显低于对照组(<0.001)。延长T4作用时间至6hRA-PBMCT4处理组TNF活性水平下降更为明显(P<0.001),表明T4可抑制PBMC体外产生TNF活性水平,其对R  相似文献   

3.
肺癌患者产生肿瘤坏死因子能力的研究   总被引:1,自引:0,他引:1  
采用生物学检测手段,对38例肺癌患者末梢血单个核细胞(PBMC)产生肿瘤坏死因子(TNF)的能力进行了研究。结果表明:肺癌患者PBMC产生TNF能力较对照组明显增高(P〈0.01),Ⅰ、Ⅱ、Ⅲ期之间PBMC产生TNF能力无明显性差异(P〉0.05),肺癌患者PBMC产生TNF能力增强,可能是机体的主动抗肿瘤免疫反应。  相似文献   

4.
采用ELISA法对33例肺癌和28例健康人外周血单个核细胞(PBMC)分泌可溶性白细胞介素-2受体(sIL-2R)能力的研究显示:肺癌患者PBMC分泌sIL-2R能力明显高于健康人(P〈0.01)。但鳞癌、腺癌、小细胞未分化癌之间,PBMC分泌SIL-2R能力无差别,三者分别较显著增高(P〈0.01)。在肺癌Ⅰ、Ⅱ、Ⅲ、Ⅳ期之间,PBMC分泌SIL-2R能力亦显显著性差异。  相似文献   

5.
采用ELISA法对33例肺癌和28例健康人外周血单个核细胞(PBMC)分泌可溶性白细胞介素-2受体(sIL-2R)能力的研究显示:肺癌患者PBMC分泌;IL-2R能力明显高于健康人(P<0.01)。但鳞癌、腺癌、小细胞未分化癌之间,PBMC分泌sIL-2R能力无差别,三者分别较健康人显著增高(P<0.01)。在肺癌Ⅰ、Ⅱ、Ⅲ、Ⅳ期之间,PBMC分泌sIL-2R能力亦无显著性差异。  相似文献   

6.
对30例直肠癌患者血清可溶性白细胞介素2受体(sIL-2R)和肿瘤坏死因子(TNF)表达水平进行了动态及临床分期观察,并以30例正常人作为对照,结果表明,直肠癌患者血清sIL-2R及TNF水平较正常人显著升高(P〈0.01),其中DukesC,D期SIL-2R水平又明显高于DukesA,B期(P〈0.010。手术治疗一周后,sIL-2R未见下降,患者机制仍处于免疫抑制状态。提示手术后患者免疫功能的  相似文献   

7.
目的 探讨食管癌、贲门癌患者围期外周血T淋巴细胞亚群、sIL-2R、TNF-β改变的规律性、相关因素及临床意义。方法 应用SAP法、ELISA法及ABC-ELISA法分别检测32例食管癌、22例贲门癌患者术前、术后1、2、3周外周血T淋巴细胞亚群、sIL-2R及TNF-β表达,并将所得结果与正常对照组作对比研究。结果 与正常对照组比较,食管癌、贲门癌患者外周血CD3^+、CD4^+明显减少,而CD8^+明显增加,CD4^+/CD8^+比值明显下降(P〈0.01),sIL-2R及TNF-β水平明显升高(P〈0.01)。患者免疫功能损害程度与肿瘤TNM分期相关。手术创伤使机体免疫功能在术后短期内受到抑制。Ⅲ期手术探查组患者与同期手术根治组患者比较,sIL-2R及TNF-β水平显著增加(P〈0.01)。结论 食管癌、  相似文献   

8.
对30例直肠癌患者血清可溶性白细胞介素2受体(sIL-2R)和肿后坏死日子(TNF)表达水平进行了动态及临床分期观察,并以30例正常人作为对照。结果表明:直肠癌患者血清sIL-2R及TNF水平较正常人显著升高(P<0.01),其中DukesC,D期SIL-2R水平又明显高于DukesA,B期(P<0.0l)。手术治疗一周后,sIL一2R未见下降,患者机体仍处于免疫抑制状态。提示手术后患者免疫功能的恢复可能是一个较为缓慢的过程。  相似文献   

9.
对36例子宫内膜异位症(Endomtrisis EMS)患者的外周血及腹腔液进行了sIL-2R、TNF-α及T细胞亚群的检测,与对照组相比,EMS患者sIL-2R、TNF-α水平明显升高(P〈0.01).T细胞亚群CD4^+及CD4^+/CD8^+经值明显升高(P〈0.01)。实验结果显示EMS患者免疫调节功能紊乱。  相似文献   

10.
检测6例活动期、6例缓解期系统性红斑狼疮(SLE)患者和10例正常人血清和外周血单个核细胞(FBMC)自发及经天然DNA(nDNA)、重组白介素2(rIL-2)和重组γ干扰素(rIFN-γ)锈导后分泌IgG类抗双链DNA(dsDNA)特异性抗体水平,观察到活动期患者血清中含量和自发分泌量显著高于缓解期,后者又显著高于正常人,且体外细胞分泌水平与血清中含量密切相关(r=0.9882)。nDNA能特异地激发SLB患者的PBMXC分泌抗dsDNA抗体,但对正常人无作用。rIL-2和rIFN-γ皆能增强SLE患者PBMC产生抗deDNA抗体。nDNA和rIL-2联合应用时对活动期患者PBMC分泌抗dsDNA抗体有协同作用。作者未发现重组白介素6(rIL-6)对抗dsDNA抗体分泌的影响。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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