汉防己甲素抑制大鼠支气管肺泡灌洗液中SRS－A的活性

作者比较了大鼠在呼吸室内空气时及急性缺氧性肺动脉高压时支气管肺泡灌洗液（ＢＡＬＦ）中慢反应物质－Ａ（ＳＲＳ－Ａ）的生物活性，以及汉防己甲素对ＳＲＳ－Ａ活性的影响。结果表明，呼吸室内空气大鼠ＢＡＬＦ中未发现ＳＲＳ－Ａ活性；急性缺氧刺激使ＳＲＳ－Ａ活性明显增强；汉防己甲素（１０－５ｍｍｏｌ／Ｌ）可抑制ＳＲＳ－Ａ的活性。我们认为：大鼠急性缺氧性肺动脉高压的形成与肺内ＳＲＳ－Ａ合成增强有关；汉防己甲素降低缺氧性肺动脉高压作用的机理涉及时ＳＲＳ－Ａ活性的抑制。     

汉防己甲素对正常人及高血压病患者血液动力学作用的研究

运用漂浮导管热稀释法监测汉防己甲素（简称汉甲）对１０名健康受试者及１２例高血压病患者的急性血液动力学作用。结果：对正常人，汉甲碱慢心率（ＨＲ），升高平均肺动脉压（ＭＰＡＰ）和肺毛细血管嵌顿压（ＰＣＷＰ），而平均动脉压（ＭＡＰ）、心输出量（ＣＯ）、心脏指数（ＣＩ）用药前后差异无显著性。对高血压病患者，汉甲明显降低ＭＡＰ、ＭＰＡＰ、ＰＣＷＰ，明显升高ＣＯ、ＣＩ，对ＨＲ无影响。提示汉甲具有潜在负性变力性     

汉防己甲素抑制一氧化氮合酶活性防治肝硬化门脉高压症

为探讨汉防己甲素抑制肝纤维化和改善门脉高动力循环的作用机制，分３组测定肝组织中一氧化氮合酶（ＮＯＳ）活性和血清中一氧化氮（ＮＯ），观察门静脉血流量（ＰＶＦ）、门静脉压力（ＰＶＰ）、门静脉阻力（ＰＶＲ）及肝脏病量学改变。结果显示，钙拮抗剂汉防己甲素显著抑制ＮＯＳ活性和ＮＯ生成，明显降低ＰＶＰ和ＰＶＦ，病理切片显示汉防己甲素明显防止肝细胞坏死，阻止肝纤维化发展。提示抑制ＮＯＳ活性和ＮＯ生成是汉防己甲素防治肝硬化门脉高压症的主要作用机理。     

汉防己甲素对犬缺氧性肺动脉高压模型血流动力学的作用及对血气水平的影响

本文研究了汉防己甲素（ＴＥＴ）对犬缺氧性肺动脉高压时肺循环血流动力学及血气的影响。在急性肺泡缺氧犬模型，静脉注射ＴＥＴ使缺氧引起的肺动脉高压恢复４２％，但对Ｐｓａ无显著性影响，ＴＥＴ仪由缺氧引起下降的ＣＯ及升高的ＴＰＶＲ和ＳＶＲ恢复到接近呼吸室内空气时的水平。ＴＥＴ对动脉血无分压（ＰａＯ２）和血氧饱和度（ＳａＯ２）无形响，但由于其使ＣＯ升高，故明显增大氧搬运系数（ＴＯ２）．直线回归分析在明：ＴＥＴ降低肺动脉高压的作用与缺氧时肺动脉压升高的水平密切相关。我们的结论是：在缺氧性肺动脉高压犬，ＴＥＴ能明显降低升高的肺动脉压和肺血管阻力，并能提高心输出量和氧搬运能力，而对系统循环和血气水平无明显影响。     

汉防己甲素对正常人及高血压病患者血液动力学作用的研究

运用漂浮导管热稀释法监测汉防己素（简称汉甲）对１０名健康受试者及１２例高血压病患者的急性血液动力学作用。结果：对正常人，汉甲减慢心率（ＨＲ），升高平均肺动脉压（ＭＰＡＰ）和肺毛细血管嵌顿压（ＰＣＷＰ），而平均动脉压（ＭＡＰ）、心输出量（ＣＯ）、心脏指数（ＣＩ）用药前后差异无显著性。对高血压病患者，汉甲明显降低ＭＡＰ、ＭＰＡＰ、ＰＣＷＰ，明显升高ＣＯ、ＣＩ，对ＨＲ无影响。提示汉甲具有潜在负性变力性与负性变时性效应，对正常血压无影响；但对高血压病患者，汉甲在安全有效降压的同时，能促进左室功能的改善。     

汉防己甲素对缺氧性肺动脉高压大鼠肺血管形态学改变的影响

为考察汉防己甲素对常压低氧性肺动脉高压大鼠肺血管形态学改变的影响，作者采用缺氧性肺动脉高压大鼠模型研究汉防己甲素处理后肺小动脉的光镜和电镜改变，并进行形态计量学分析。结果显示缺氧性肺动脉高压大鼠肺小动脉变细，平滑肌细胞增生。图象分析发现肺小动脉外径变小，管壁厚度占外径的比例增大，血管壁面积占血管总面积增大，经汉防己甲素处理后，上述病变明显减轻。由此表明，汉防己甲素主要通过抑制肺小动脉胶原纤维的增生和抑制缺氧引起的肺血管增厚和肌化，使肺小动脉管腔狭窄明显减轻，肺动脉压降低，从而部分阻抑肺动脉高压的发展     

汉防己甲素治疗野百合碱诱发大鼠肺动脉高压的作用研究

以野百合碱复制Ｗｉｓｔａｒ大鼠肺动脉高压模型，观察汉防己甲素对其血流动力学的作用及对肺腺泡内动脉的影响。结果表明：汉防己甲素可使肺动脉收缩压、右心室收缩压及右心房收缩压分别降低２８．０％、２８．３％及３１．５％；并使肺腺泡内动脉内皮细胞空泡变减少，内皮下腔减少和缩小，中膜胶原纤维和平滑肌细胞亦减少，使其管壁变薄。     

汉防己甲素治疗野百合碱诱发大鼠肺动脉高压的作用研究

以野百合碱复制Ｗｉｓｔａｒ大鼠肺动脉高压模型，观察汉防己甲素对其血流动力学的作用及对肺腺泡内动脉的影响。结果表明：汉防己甲素可使肺动脉收缩压，右心室收缩压及右心房收缩压分别降低２８．０％、２８．３％及３１．５％，并使肺腺泡内动脉内皮细胞空泡变减少，内皮下腔减少和缩小，中膜胶原纤维和平滑肌细胞亦减少，使其管壁变薄。     

应用肺阻抗微分波测定肺动脉压的初步探讨

本文用肺阻抗微分波测定４６例肺心病患者的肺动脉平均压（ｍＰＡＰ），肺动脉收缩压（ＰＡＳＰ），肺动脉舒张压（ＰＡＤＰ）并与３２例正常人的肺劝脉压对照。发现肺心组与正常组，ｍＰＡＰ、ＰＡＳＰ、ＰＡＤＰ人有显著差异性。４６例肺心病病人有４３例符合肺动脉高压标准，对照组肺动脉压均在正常范围，肺阻抗微分波图的临床应用，为无创伤性估测肺动脉压提供了一种新方法，该法能直接反映血流情况，故其指标与肺动脉压相关性较     

培哚普利抑制慢性缺氧高二氧化碳大鼠肺动脉高压的形成

研究慢性低氧高二氧化碳性肺动脉高压大鼠模型的血浆血管紧张素Ⅱ（ＡｎｇⅡ）的变化与平均肺动脉压（ｍＰＡＰ）的关系，并观察血管紧张素转换酶抑制剂（ＡＣＥＩ）—培哚普利的干预作用。实验分五组。结果：①缺氧四周组（Ｆ１）与八周组（Ｅ１）ＡｎｇⅡ浓度和ｍＰＡＰ都比正常对照组（Ｃ）明显升高（Ｐ＜０．０１），且两者成正相关（ｒ＝０．８１１，Ｐ＜０．０１）。②相对应的用培哚普利四周组（Ｆ２）与八周组（Ｅ２），其ＡｎｇⅡ与ｍＰＡＰ分别比Ｆ１组、Ｅ１组低（Ｐ＜０．０５，Ｐ＜０．０１），与Ｃ组相比无升高（Ｐ＞０．０５）。提示：慢性缺氧高二氧化碳大鼠肺动脉压升高与ＡｎｇⅡ有关，培哚普利有阻抑肺动脉高压形成的作用。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

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<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.