水杨梅化学成分的研究

从水杨梅根中分得８个成分，经光谱和化学方法鉴定为：ｑｕｉｎｏｖｉｃ ａｃｉｄ（Ｉ），３－ｏｘｏ－ｕｒｓ－１２－ｅｎｅ－２７，２８－ｄｉｏｉｃ ａｃｉｄ（Ⅱ），ｑｉｎｏｖｉｃ ａｃｉｄ－３β－Ｏ－β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｉｄｅ（Ⅲ），ｑｕｉｎｏｖｉｃ ａｃｉｄ－３－β－Ｏ－α－Ｌ－ｒｈａｍｎｏｐｙｒａｎｏｓｉｄｅ（Ⅳ），ｎｏｒｅｕｇｅｎｉｎ（Ⅴ），７－Ｏ－β－Ｄ－ｇｌｕｃｏｓｙｌ－ｎｏｒｅｕ     

Synthesis of Glycoproteins by Mucosa of Gastric Antrum in Chronic GastritisB

ＳｙｎｔｈｅｓｉｓｏｆＧｌｙｃｏｐｒｏｔｅｉｎｓｂｙＭｕｃｏｓａｏｆＧａｓｔｒｉｃＡｎｔｒｕｍｉｎＣｈｒｏｎｉｃＧａｓｔｒｉｔｉｓＢＴＩＡＮＤｅ－ａｎ（ＤｅｐａｒｔｍｅｎｔｏｆＧａｓｔｒｏｅｎｔｅｒｏｌｅｇｙ，ＴｏｎｇｊｉＨｏｓｐｉｔａｌ，Ｔｏｎｇｊ...     

日本七叶树化学成分的研究Ⅰ.七叶皂苷Ⅰa和Ⅰb的分离和鉴定

从日本七叶树 Ａｅｓｃｕｌｕｓ ｔｕｒｂｉｎａｔａ Ｂｌｕｍｅ．种子的 ７０％乙醇提取物中分得 ４个三萜皂苷化合物,经光谱学分析鉴定了其中 ２个化合物的结构,它们分别为：七叶皂苷Ⅰａ：２１β－巴豆酰基－２２ａ－乙酸基原七叶皂苷配基－３β－Ｏ－［β－Ｄ－吡喃葡萄糖基（１－２）］［β－Ｄ－吡喃葡萄糖基（１－４）］－β－Ｄ－吡喃葡萄糖醛酸（２１β－ｔｉｇｌｏｙｌ－２２α－ａｃｅｔｙｌｐｒｏｔｏａｅｓｃｉｇｅｎｉｎ－３β－Ｏ－［β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｙｌ（１－２）］［β－Ｄ－ｇｌｕｃｏｐｙｒａ－ｎｏｓｙｌ（１－４）］－β－Ｄ－ｇｌｕｃｏｐｙｒａ－ｎｏｓｉｄｕｒｏｎｉｃ　ａｃｉｄ）和七叶皂苷Ⅰｂ：２１β－当归酰基－２２α－乙酰基原七叶皂苷配基－３β－Ｏ－［β－Ｄ－吡喃葡萄糖基（１－２）］［β－Ｄ－吡喃葡萄糖基（１－４）］－β－Ｄ－吡喃葡萄糖醛酸（２１β－ａｎ－ｇｅｌｏｖｌ－２２ａ－ａｃｅｔｙｌｐｒｏｔｏａｅｓｃｉｇｅｎｉｎ－３β－Ｏ－［β－Ｄ－ｇｌｕｃｏｎｙｒａｎｏｓｙｌ（１－２）］［β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｙｌ（ｌ－４）］－β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｉｄｕｒｏｎｉｃ ａｃｉｄ）,均为首次从日本七叶树     

Die Glykoproteinsynthese antraler Magenschleimhaut beichronischer Gastritis Typ B

ＤｉｅＧｌｙｋｏｐｒｏｔｅｉｎｓｙｎｔｈｅｓｅａｎｔｒａｌｅｒＭａｇｅｎｓｃｈｌｅｉｍｈａｕｔｂｅｉｃｈｒｏｎｉｓｃｈｅｒＧａｓｔｒｉｔｉｓＴｙｐＢＴＩＡＮＤｅ－ａｎ（田德安）（ＡｂｔｅｉｌｕｎｇｆｕｒＧａｓｔｒｏｅｎｔｅｒｏｌｏｇｉｅ，Ｔｏｎｇｊｉ...     

Current Titles in Chinese

ＣｕｒｒｅｎｔＴｉｔｌｅｓｉｎＣｈｉｎｅｓｅＢａｓｉｃＲｅｓｅａｒｃｈ００４８５ＹａｎｇＲｅｎ－－ｚｈｕ（ｔｅＰＤ￥），ｅｔａｌ：ＣｈａｎｇｅｓｏｆＣａＭｌｅｖｅｌｉｎｒａｔｈｙＰＯｔｈａｌａｍｕｓ，ｏｖａｒｙａｎｄｕｔｅｒｕｓｏｆｒａｔｉｎａｃｃｏ...     

The Activity of Plasma Platelet－activating Factor Derived from Pregnant Women before and after Delivery and lts Relation to P

ＴｈｅＡｃｔｉｖｉｔｙｏｆＰｌａｓｍａＰｌａｔｅｌｅｔ－ａｃｔｉｖａｔｉｎｇＦａｃｔｏｒＤｅｒｉｖｅｄｆｒｏｍＰｒｅｇｎａｎｔＷｏｍｅｎｂｅｆｏｒｅａｎｄａｆｔｅｒＤｅｌｉｖｅｒｙａｎｄｌｔｓＲｅｌａｔｉｏｎｔｏＰｒｅｇｎａｎｃｙ－ｉｎｄｕｃｅｄＨｙ...     

Effects of 3，4－Dihydroxyacetophenone （3，4－DHAP） on Hypoxic Pulmonary and Systemic Vascular Response in Dogs

（王迪浔）（明志）（余上斌）Ｅｆｆｅｃｔｓｏｆ３，４－Ｄｉｈｙｄｒｏｘｙａｃｅｔｏｐｈｅｎｏｎｅ（３，４－ＤＨＡＰ）ｏｎＨｙｐｏｘｉｃＰｕｌｍｏｎａｒｙａｎｄＳｙｓｔｅｍｉｃＶａｓｃｕｌａｒＲｅｓｐｏｎｓｅｉｎＤｏｇｓ￥ＦａｒｍａｎＵｌｌａｈ；ＷＡＮ...     

The production of Strains Highly Expressing Human Interleukin-2 cDNA

(张大军)(冯博)(皇甫永穆)ＴｈｅｐｒｏｄｕｃｔｉｏｎｏｆＳｔｒａｉｎｓＨｉｇｈｌｙＥｘｐｒｅｓｓｉｎｇＨｕｍａｎＩｎｔｅｒｌｅｕｋｉｎ－２ｃＤＮＡ￥ＺＨＡＮＧＤａ－ｊｕｎ，ＦＥＮＧＢｏ，ＨＵＡＮＧＦＵＹｏｎｇ－ｍｕ（ＤｅｐａｒｔｍｅｎｔｏｆＭｅｄｉｃａ...     

女贞子化学成分的研究（Ⅱ）~（1））

继前报，作者又从女贞子（ＬｉｇｕｓｌｒｕｍＬｕｃｉｄｕｍＡｉｌ．）乙醇提取物的石油醚萃取部分和乙酸乙酯萃取部分分离鉴定了４个化合物．化合物Ⅵ为１９α－羟基－３－乙酰乌索酸（１９α－ｈｙｄｒｏｘｙ－３－ａｃｅｔｙｌ－ｕｒｓｏｌｉｃａｃｉｄ），化合物Ⅸ为对羟基苯乙醇－β－Ｄ－葡萄糖甙（ｐ－ｈｙｄｒｏｘｙｐｈｅｎｅｔｈｙｌ－β－Ｄ－ｇｌｕｃｏｓｉｄｅ），化合物Ｘ为芹菜素－７－Ｏ－β－Ｄ－葡萄糖甙（ｃｏｓｍｏｓｓｉｎ），化合物Ⅺ为甘露醇（ｍａｎｎｉｔｏｌ）．其中化合物Ⅵ为一新天然产物．     

Percutaneous Cervical Disecectomy for Treating Cervical Disc Herniation──A report of 12 Cases

ＰｅｒｃｕｔａｎｅｏｕｓＣｅｒｖｉｃａｌＤｉｓｅｃｅｃｔｏｍｙｆｏｒＴｒｅａｔｉｎｇＣｅｒｖｉｃａｌＤｉｓｃＨｅｒｎｉａｔｉｏｎ──Ａｒｅｐｏｒｔｏｆ１２ＣａｓｅｓＺＨＯＵＹｉ－ｃｈｅｎｇ（周义成），ＺＨＯＵＹｕｎ－ｑｉｎｇ（周韵清），ＷＡＮＧＣｈｅ...     

二氢石蒜碱对家兔心室乳头肌及血管平滑肌收缩的影响

目的 :观察二氢石蒜碱 (dihydrolycorine ,DL)对家兔心室乳头肌的作用。方法 :采用家兔离体右心室乳头肌(PM)、胸主动脉 (TA)及基底动脉 (BA)标本 ,记录药物对其张力的影响。结果 :DL或Nim对电刺激诱发PM的收缩均有剂量依赖性抑制作用 ,其IC50 值分别为 (1.4 5± 0 .89)× 10 -3 mol·L-1、(1.30± 0 .4 5 )× 10 -6mol·L-1;KCl(6 0mmol·L-1)、NA(0 .1mmol·L-1)所致TA及BA收缩 ,DL使之呈剂量依赖性松弛 ,其IC50 值对TA分别为 (2 .91± 0 .99)× 10 -3mol·L-1、(1.4 9± 0 .5 9)× 10 -3 mol·L-1;对BA分别为 (3.4 1± 1.5 2 )× 10 -3 mol·L-1、(6 .6 9± 3.12 )× 10 -4mol·L-1,DL拮抗NA所致基底动脉收缩作用明显强于KCl(P <0 .0 1)。Nim对KCl所致收缩的抑制显著强于对NA(P <0 .0 1) ;DL对电刺激诱发PM收缩的IC50 值低于KCl所致TA、BA收缩IC50 值 ,且显著高于NA所致BA收缩IC50 值 ,低于NA所致TA收缩IC50 值。结论 :DL对电刺激所致PM的收缩具有抑制作用 ,其作用机制可能为抑制PDC、ROC ,且抑制作用ROC >PDC。     

高碳酸抑制兔离体脑动脉缺氧性收缩

在离体兔基底动脉观察了ＣＯ_２、ｐＨ值对血管缺氧反应的影响。在以５－羟包胺（１０~（－６）ｍｏｌ／Ｌ）预收缩后，缺氧使血管张力升高１１４．８７±４０．７５ｍｇ，Ｔ％为４８．６２±１２．１１（ｎ＝５１）。去内皮对缺氧性收缩无影响。随浴槽液中ＣＯ_２浓度增加，缺氧性收缩幅度减弱，在Ｐ_∞_２为８．００、９．３３、１０．６７ｋＰａ时，Ｔ％仅分别为３８．３０±５．３６％（ｎ＝１１）、３２．２５±７．７６％（ｎ＝１３）、２９．９０±９．２６％（ｎ＝１１），较对照值（Ｐ_∞_２＝５．３３ｋＰａ）均有显著差异（Ｐ＜０．０１）。ｐＨ７．３～７．７时血管缺氧反应无显著变化。一氧化氮合成酶抑制剂Ｌ—ＮＮＡ（１０~６）可抑制血管的缺氧收缩反应，也抑制了不同ＣＯ_２浓度对缺氧反应的影响，结果提示ＣＯ_２可能经促血管分泌ＮＯ的功能而抑制了缺氧性收缩。     

PI3K/Akt信号通路在缺氧性肺血管收缩中的机制

目的：探讨15-羟基二十碳四烯酸（15-HETE）致大鼠肺动脉收缩的信号转导途径,阐明15-HETE引起慢性缺氧性肺动脉收缩作用的可能机制。方法：采用组织浴槽血管环方法观察15-HETE对缺氧和正常组大鼠血管收缩的作用,同时观察PI3K/Akt抑制剂LY294002对肺动脉环收缩强度的改变,明确PI3K/Akt信号转导途径在15-HETE收缩肺动脉中的作用。结果：15-HETE对正常组和缺氧模型组大鼠血管环均有收缩作用,加入AKT抑制剂LY294002可明显阻断15-HETE对缺氧大鼠肺动脉的收缩作用（P〈0.05）。结论：15-HETE通过AKT信号转导途径收缩慢性缺氧性大鼠肺动脉。     

Effects of 3, 4-Dihydroxyacetophenone on Cytosolic Calcium in Pulmonary Artery Endothelial and Smooth Muscle Cells during Acute Hypoxia

Summary: The effects of 3, 4-Dihydroxyacetophenone (3. 4-DHAP) on cytosolic free calcium[Ca^2 ], in pulmonary artery endothelia (PAECs) and smooth muscle cells (PASMCs) during acute hypoxia were studied. Porcine pulmonary artery endothelial and smooth muscle cells (PASMCs)were cultured primarily, and they were divided into 4 groups: groups incubated under normoxia or hypoxia and those with or without treatment with 3. 4-DHAP. The [Ca^2 ]i of both PAECs and PASMCs was measured by determining the fluorescence of fura 2 AM on spetrofluorometer. Our results showed that hypoxia caused significant elevation of [Ca^2 ]i. in both PAECs and PASMCs,3, 4-DHAP could attenuate the hypoxic elevation of [Ca^2 ]i only in PASMCs but not in PAECs. It is concluded that 3, 4-DHAP decreases the hypoxic elevation of [Ca^2 ]i in PASMCs. This might contribute to its inhibitory effect on hypoxic pulmonary vasoconstriction.     

Effect of acetylcholine on membrane potential in toad dorsal root ganglion neurons and its underlying ionic basis

Intracellular recordings were made to investigate the responses of membrane potential to acetylcholine (ACh) on neurons in isolated toad dorsal root ganglion (DRG). In the 73 neurons examined, 67 were of type A, and the remaining 6 of type C cell. The resting membrane potential of these two types of cells was −67.5±1.3 mV (× ±SE). During the application of ACh (4 × 10^-4–6 × 10^-4 mol/L), the changes in membrane potential were as follows: 1) hyperpolarization, with amplitude of 9.1±3.0 mV (X ± SE; n = 23); 2) depolarization, with amplitude of 12.9 ±2.2 mV (X ±SE; n = 20); 3) biphasic response, i.e., hyperpolarization with amplitude of 8.0±2.4 mV (X±SE) followed by depolarization with amplitude of 10.9±2.1 mV (X±SE) (n=24); no effect (n=6). The hyperpolarization induced by ACh was blocked by superfusion with atropine (1.3 × 10^-5 mol/L; n = 23), while ACh depolarization was blocked by the mixture of d-tubocurarine (1.4 × 10^-5 mol/L) and hexamethonium (1.4 ×10^-5 mol/L) (n = 18). When ACh caused hyperpolarization, the membrane conductance wascin reased by 13.8% and the reversal potential was about -96 mV (n=3). TEA (20 mmol/L) superfusion enhanced ACh depolarization amplitude by 48.2 ±3.2 % (× ± SE;n = 6), and depressed ACh hyperpolarization amplitude by 79.4 ±4.3 % (× ± SE; n= 8).MnCl₂ (4 mmol/l) superfusion decreased the amplitudes of ACh depolarization and hyperpolarization by 54.2 ±7.2 % (X ±SE; n= 5) and by 69.2±6.4 % (X±SE; n = 14) respectively. These results suggest that the depolarization and hyperpolarization induced by ACh are mediated by nicotinic and muscarinic receptors on the soma of toad DRG neurons separately, and it seems that ACh hyperpolarization involves activation of calcium-activated potassium conductance.     

用肺组织条作缺氧性肺血管收缩反应研究的探索

目的　探索由急性缺氧所致肺组织条张力变化与肺动脉和气管张力变化的关系 ,以探讨用肺组织条研究缺氧性肺血管反应的可行性。方法　因为肺组织条中可收缩的部分主要是血管和气管 ,所以分别做肺组织条、肺动脉、气管的张力实验。纵向剪取Wistar大鼠肺组织条以及肺动脉环 (肺动脉三级分支制成的肺动脉环 )和肺内段气管条悬挂于张力传感器上 ,记录张力变化。结果　急性缺氧使肺组织条及肺动脉环张力增加 ,而气管条张力下降 ,在加入消炎痛阻断前列腺素生成后 ,肺组织条缺氧性收缩反应增强 ,肺血管环缺氧反应也增加 ,而对肺内气管条的缺氧反应无明显影响 ;在加入L -NAME阻断一氧化氮生成后 ,肺组织条和肺血管环缺氧性收缩反应均增强 ,而对肺内气管条的缺氧反应无明显影响 ;在加入 4-AP阻断电压门控钾通道后 ,肺组织条与肺血管环缺氧性收缩反应均下降 ,气管缺氧性舒张反应减弱。结论　必要时可用肺组织条作缺氧性肺血管反应性研究     

Comparison of loading with maintenance dose of clopidogrel on platelet reactivity in Chinese with different CYP2C19 genotypes prior to percutaneous coronary intervention

Background Whether two clopidogrel pretreatment strategies prior to elective percutaneous coronary intervention （PCI）： a 300 mg loading dose （LD） in clopidogrel naive patients and a 75 mg maintenance dose （MD） once daily in patients on chronic clopidogrel therapy play the same role in the platelet inhibition in Chinese with different CYP2C19 genotypes remains unknown. We aim to evaluate the impact on platelet inhibition by clopidogrel pretreatment strategy and its interaction effect with CYP2C19 genotype. Methods Chinese patients undergoing PCI （n=840） were assigned to 2x2 groups in the trial according to different clopidogrel pretreatment strategies （470 patients in LD, 370 patients in MD） and CYP2C19 genotypes （494 carriers of any CYP2C19 ＊2 or ＊3 loss-of-function allele, 346 non-carriers）. The primary outcome was platelet aggregation （PA） as measured by the 10 umol/L adenosine diphosphate induced light transmission aggregation. Results Compared with MD group, LD strategy showed a significantly higher PA-（（59.22＋11.67）% vs. （52.83±12.17）%, P 〈0.01）, similar PA difference was observed in CYP2C19 loss-of-function carriers compared with non-carriers （（59.41±10.91）% vs. （52.10±12.90）%, P 〈0.01）. LD patients in either the CYP2C19 loss-of-function allele carrier or non- carrier group showed a significantly higher PA compared with MD group （（61.50±10.61）% vs. （56.84±10.74）%, P 〈0.01; （56.06±12.34）% vs. （46.88±11.78）%, P 〈0.01, respectively）. A quantitative interaction effect was observed between clopidogrel pretreatment strategy and CYP2C19 genotype （P=0.001）. Conclusion The 300 mg LD strategy results in a decreased effect on platelet inhibition compared with the 75 mg MD in Chinese patients receiving clopidogrel prior to PCI, especially in the CYP2C19 ＊2 or ＊3 loss-of-function allele non-carriers. （ClinicalTrials.gov number NCT01710436） Chin tided J 2014;127 （14）： 2571-2577     

心房组织中5-HT4受体亚型mRNA的变化与房颤发生与维持

目的:探讨房颤患者房间隔5-HT4受体4种亚型(5-HT4Ra、5-HT4Rb、5-HT4Rg、5-HT4Ri)mRNA的表达及其临床意义.方法:将行瓣膜置换手术的患者36例,分为窦性心律(SR)组和房颤(AF)组.术中取房间隔组织,采用SYBR Green实时荧光定量PCR检测各组中5-HT4受体亚型mRNA的表达.结果:5-HT4Ra mRNA在SR组和AF组中的表达水平分别为(19.89±9.68)×10-4和(336.86±255.56)× 10-4,AF组比SR组增加了1 593.61%(P＜0.01);5-HT4Rb mRNA在SR组和AF组中的表达水平分别为(23.32±8.37)×10-4和(240.32±242.49)×10-4,AF组比SR组增加了930.53%(P＜0.01);5-HT4Rg mRNA在SR组和AF组中的表达水平分别为(2.23±0.77)×10-4和(0.72±0.45)× 10-4,AF组比SR组减少了67.71%(P＜0.01);5-HT4Ri mRNA在SR组和AF组中的表达水平分别为(34.95±8.08)×10-4和(392.15±347.75)×10-4,AF组比SR组增加了1 022.03%(P＜0.01).结论:5-HT4受体亚型mRNA表达的变化可能在房颤的发生和维持中发挥一定的作用.     

2型糖尿病合并冠心病患者纤溶酶原激动抑制剂-1基因多态性分析

为探讨纤溶酶原激活抑制剂 1 (PAI 1 )基因调节区 -675bp位点 4G/5G多态性与 2型糖尿病、2型糖尿病合并冠心病 (CAD)的关系 ,采用特异性引物聚合酶链反应 (PCR)方法 ,测定 4G/5G基因型 ,并检测PAI 1抗原水平 ,共观察了 1 2 1例单纯 2型糖尿病 (typeⅡdiabetes)患者、87例 2型糖尿病合并CAD患者和 1 0 0例对照者。结果 :合并CAD组的 4G/4G基因型携带者明显高于对照组 ( 4 9.4%比 3 7.0 % ,P <0 .0 5 ) ,CAD组中PAI 1与三酰甘油 (TG)呈正相关 ,并与 4G/5G多态性有关 ,表现为 4G/4G基因型携带者的PAI 1质量浓度明显高于 5G/5G基因型携带者〔( 3 9.82× 1 0 - 3± 3 .1 1× 1 0 - 3)ng/L比 ( 3 8.0 4× 1 0 - 3± 2 .73× 1 0 - 3)ng/L ,P <0 .0 5〕。提示 :PAI 1基因 4G/5G多态性与TG的联合作用与PAI 1抗原水平相关 ,并与 2型糖尿病合并CAD的发病过程有关。