SOD、脂质过氧化物、CP与自然流产的相关性

目的通过对体内超氧化物歧化酶（SOD）、脂质过氧化物及还原物质铜蓝蛋白（CP）的测定,探讨SOD、脂质过氧化物及cP与自然流产的关系。方法用Elisa法测定30例正常早孕、先兆流产、胚胎停育妇女血清中SOD水平,用化学比色法测定其脂质过氧化物水平,用酶学法测定其CP水平。结果与正常早孕妇女相比．先兆流产与胚胎停育妇女血清中脂质过氧化物水平都升高（P〈0．05）,SOD,CP水平都下降（P〈0.05）;胚胎停育与先兆流产患者相比血清脂质过氧化物水平升高（P〈0．05）,SOD,CP水平下降,但差异均无统计学意义（P〉0．05）。结论体内SOD,脂质过氧化物,CP的水平与自然流产具有相关性。     

尿毒症维持性透析患者炎症和氧化应激状态相关因素分析

目的分析尿毒症维持性透析患者炎症和氧化应激状态的相互关系及影响因素。方法分光光度法及免疫比浊法测定尿毒症患者血清超氧化物歧化酶（SOD）、维生素E（VitE）、维生素C（VitC）、丙二醛（MDA）、C-反应蛋白（CRP）。结果与非尿毒症相比，尿毒症患者SOD、VitE、VitC水平较低（P〈0．01），CRP和MDA浓度较高（P〈0．01）。腹膜透析患者SOD、VitC、VitE水平高于血仿膜透析组（P〈0．05）。与聚砜膜透析组无差异；MDA水平低于两血液透析组（P〈0．05）；CRP水平与血液透析组无差异。聚砜膜组VitC、VitE水平高于血仿膜组，SOD、MDA及CRP水平两组间无差异（P〉0．05）。结论尿毒症患者处于炎症和氧化应激状态。尿毒症炎症和氧化应激互为因果，相互作用。血液净化方式的选择对尿毒症炎症、氧化应激及羰基应激的影响尚不确定。     

尿毒症炎症、氧化应激和羰基应激状态的相关性分析

目的分析尿毒症炎症、氧化应激和羰基应激状态三者间的相互关系及不同透析方式对其的影响。方法采用分光光度法及免疫比浊法测定尿毒症患者和非尿毒症患者以及不同透析方式下尿毒症患者血清超氧化物歧化酶（SOD）、维生素E（VitE）、维生素C（VitC）、丙二醛（MDA）、C-反应蛋白（CRP）及总羰基化合物（TCC）水平。结果与非尿毒症患者相比，尿毒症患者SOD、VitE、VitC水平较低（P〈0．01），CRP、MDA及TCC较高（P〈0．01）。腹膜透析患者SOD、VitC、VitE水平高于血仿膜透析组（P〈0．05），与聚砜膜透析组差异无统计学意义IMDA、TCC水平低于两血液透析组（P〈0．05）；CRP水平与血液透析组差异无统计学意义。聚砜膜组VitC、VitE水平高于血仿膜组，SOD、MDA、CRP及透析前TCC水平两组间无差异（P〉0．05）；聚砜膜组透析前后TCC清除量高于血仿膜组（P〈0．01）。与TCC作多元回归分析所得标准化回归系数分别为MDA（b=0．727，P〈0．01）、CRP（b=0．370，P〈0．01）、SOD（b=0．192，P〈0．05）、VitC（b=-0．153，P〈0．01）、VitE（b=0．054，P〉0．05）。结论尿毒症患者处于炎症、氧化应激和羰基应激状态。尿毒症炎症、氧化应激可能是尿毒症羰基应激的重要机制。血液净化方式的选择对尿毒症炎症、氧化应激及羰基应激的影响尚不确定。     

血尿酸升高及氧化应激在冠心病发生中的作用

目的通过了解冠心病（CHD）患者血清尿酸（UA）水平、氧化应激损伤程度及其相互联系，探讨血UA升高及氧化应激损伤在CHD发生中的作用。方法选择急性冠脉综合征（ACS）、稳定型心绞痛（SAP）和对照组（CO）患者各30例，测定外周血清UA、丙二醛（MDA）和总超氧化物岐化酶（T—SOD）活力，然后进行统计学分析。结果ACS和SAP组患者的血清UA和MDA含量均高于CO组（P均〈0．05），而T—SOD活力均低于CO组（P均〈0．05）；ACS组和SA组相比，血清UA水平差异无统计学意义（P〉0．05），而血清MDA水平明显增高、T—SOD活力则明显降低（P均〈0．05）；冠心病患者的UA与MDA呈正相关（r=0．614，P〈0．05），与T—SOD活力呈负相关（r=-0．591，P〈0．05）；SAP组高尿酸血症（HUA）者与正常血UA者相比，血清T—SOD活力明显降低（P〈0．05）；ACS组HUA者与正常血UA者相比，血清MDA水平明显增高、T—SOD活力明显降低（P均〈0．05）。结论血清UA水平升高可能是CHD的一个危险因素，其可能通过促进氧化应激损伤增加，抗氧化能力降低，导致了CHD的发生发展。     

丹参对维持性血液透析患者AOPP、MDA和SOD的干预作用

目的研究透析和丹参对维持性血液透析（MHD）患者氧化应激指标的影响。方法36例MHD半年以上的患者随机分为常规透析组和透析加丹参组，同时18例健康成人作为正常对照组。分别测定各组基础以及透析组1、2和3个月的血浆氧化应激指标（AOPP、MDA和SOD）的水平。结果透析患者的AOPP、MDA明显高于正常对照组（P〈0．01）、SOD显著低于正常对照组（P〈0．01）。常规透析组3个月时AOPP、MDA较服药前增加（P〈0．05），SOD降低（P〈0．05）。丹参组在1、2和3个月时所测MDA、AOPP明显低于服药前以及同时点常规透析组（P〈0．01），而SOD则显著增高（P〈0．01），但3个月时AOPP、MDA仍高于正常对照组（P〈0．01），SOD低于正常对照组（P〈0．01）。结论丹参能有效地改善MHD患者的氧化应激，血液透析可能加重MHD患者的氧化应激。     

夹竹桃麻素对快速起搏离体兔心脏氧化应激损伤的保护作用

目的研究还原性烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶抑制剂夹竹桃麻素（APO）对快速起搏离体兔心脏氧化应激损伤的保护作用。方法采用离体兔心脏Langendorff灌注装置建立快速起掉右心房实验模型,离体兔心脏24只随机分成正常对照组、快速起搏组（RAP）和夹竹桃麻素预处理组（APO）,每组8只。正常对照组用K—H液灌注离体兔心脏80min;RAP组离体灌流30min,电极快速起搏右心房50min;APO纰用夹竹桃麻素预处理30min,快速起搏50min。所有组灌流结束后,留取心房肌组织。H—E染色检测心房肌病理变化,免疫组化和Westernblot检测心房肌组织中蛋白表达并测定超氧化物歧化酶（SOD）、丙二醛（MDA）的变化。结果①正常对照组心肌细胞排列整齐,结构正常;RAP组心肌细胞排列紊乱,大量心肌细胞溶解;APO组心肌细胞排列较RAP组整齐,可见部分心肌细胞溶解;②与正常对照组比,RAP组心房肌钙激活蛋白酶calpainl表达增加（P〈0．05）,SOD蛋白和肌钙蛋白T（cTnT）表达减少（P〈0．05）;与RAP组比,APO组心房肌calpainl蛋白表达减少（P〈0．05）,SOD蛋白和cTnT蛋白表达均增加（P〈0．05）;③与正常组比,RAP组心房肌MDA水平明显增加（P〈0．05）,SOD水平明显减少（P〈0．05）;与RAP组比,APO组心房肌MDA水平减少（P〈0．05）,SOD水平增加（P〈0．05）。结论夹竹桃麻素可以通过抑制氧化应激,减轻快速起搏离体兔心房导致的心肌氧化应激损伤。     

口服VitE对维持性血液透析患者氧化应激的影响

目的 探讨VitE对维持性血液透析（MHD）患者氧化应激和微炎症状态的影响。方法 40例MHD1年以上的患者随机分为常规透析组（A组）和透析加口服VitE对照组（B组）。分别测定两组基础以及分组干预后1个月的血浆氧化应激指标（MDA）和微炎症指标（CRP、IL-6）的水平。结果 两组患者的基础MDA无显著差异（P〉0．05）。分组干预1个月后，常规透析组MDA较基础值增加（P〈0．05），CRP、IL-6增加但无显著性差异（P〉0．05），透析加Vit E组在1个月时MDA、CRP、IL-6明显低于基础值以及同时点常规透析组（P〈0．01）。结论 口服VitE能有效地改善MHD患者的氧化应激和微炎症状态。     

支原体感染与稽留流产

目的研究支原体感染与稽留流产的关系。方法采用Bact-IST支原体分析系统和PER方法，分别对49例（研究组）稽留流产的患者和正常早孕人流患者63例（对照组）的宫颈分泌物和绒毛组织进行检测，观察其支原体感染率。结果研究组支原体感染率分别为46．94％和26．98％，对照组分别为36．73％和14．29％，两者比较P〈0．05和P〈0．01，有显著性差异。结论生殖道支原体感染与稽留流产具有明显的相关性。     

细胞凋亡及凋亡相关基因bcl-2和bax在胚胎停止发育绒毛组织中的表达

目的研究细胞凋亡及凋亡相关基因bcl-2、bax在胚胎停止发育绒毛组织中的表达。方法采用TUNEL技术和免疫组织化学SP法对40例正常早孕绒毛组织和40例胚胎停止发育绒毛组织进行凋亡细胞和bcl-2、bax表达测定。结果胚胎停止发育绒毛组织中凋亡细胞显著增加，与正常早孕绒毛组织比较有显著性差异（P〈0．05）；胚胎停止发育绒毛组织中bcl-2表达低于正常早孕绒毛组织，而bax表达高于正常早孕绒毛组织，两者均有显著性差异（P〈0．05）。结论胚胎停止发育绒毛组织细胞凋亡指数显著增加，且与bcl-2的表达呈负相关，与bax的表达呈正相关。     

短期NCPAP治疗对OSAHS患者氧化应激相关指标的影响

目的观察短期经鼻持续气道正压通气（NcPAP）治疗对阻塞性睡眠呼吸暂停低通气综合征（0SAHS）患者血清氧化应激指标水平的影响。方法选择中、重度OSAHS患者43例和正常对照组24例,比较两组空腹静脉血超氧化物歧化酶（SOD）、硫氧还蛋白-1（TRX-1）及丙二醛（MDA）水平。对OSAHS患者行NCPAP治疗7d,再测上述血清指标并与治疗前比较。结果与对照组比较,OSAHS组血清MDA及TRX-1水平增高,SOD水平降低,差异均有统计学意义（均P〈0．05）。OSAHS组经过7dNCPAP治疗后,TRX-1、MDA及SOD水平与治疗前差异均有统计学意义（均P〈0．05）;与对照组TRX-1、MDA差异无统计学意义（均P〉0．05）,SOD水平有所升高但与对照组仍有统计学意义（P〈0．05）。结论OSAHS是一种氧化应激性疾病,而及早给予短期NCPAP治疗可改善缺氧程度,逆转氧化应激反应。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.