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1.
李植飞  唐辉  唐祖年 《华夏医学》2013,(6):1057-1060
目的:探讨野菊花不同提取物对大鼠、小鼠、家兔离体子宫平滑肌的兴奋作用.方法:采用常规方法制备动物离体子宫实验材料,用BL-420F生物机能实验系统记录离体子宫平滑肌的活动,以子宫收缩的幅度、频率及收缩力作为指标,观察野菊花提取物对注射己烯雌酚后大鼠、小鼠、家兔离体子宫平滑肌的影响,计算兴奋子宫的IC50.结果:野菊花正丁醇组分、水溶性组分对大鼠、小鼠、家兔离体子宫平滑肌均有明显的兴奋作用,收缩的幅度增高,频率加快,张力增加,并呈量效关系;石油醚组分仅对家兔离体子宫平滑肌有兴奋作用,而对大鼠和小鼠离体子宫平滑肌无兴奋作用.结论:野菊花提取物正丁醇组分及水溶性组分对大鼠、小鼠和家兔离体子宫平滑肌有明显兴奋作用.  相似文献   

2.
目的观察不同浓度甜茶提取液对家兔离体小肠的收缩活动及对小鼠在体小肠推进运动的影响。方法采用离体肠段实验法和小肠推进运动实验法,观察甜茶提取液对家兔离体小肠收缩活动及小鼠在体小肠推进运动的效应。结果三种浓度甜茶提取液(终浓度:2×10-6g/L、4×10-6g/L、6×10-6g/L),对家兔不同部位离体小肠平滑肌的收缩运动均有抑制作用,使其张力降低,收缩幅度变小(P<0.01),而高剂量组对收缩频率有减少作用(P<0.05);对小鼠在体小肠推进运动有明显的抑制作用(P<0.01)。结论甜茶提取液对离体小肠平滑肌的收缩运动、小鼠在体小肠推进运动均具有明显抑制作用。  相似文献   

3.
目的研究陈皮提取物对在体小鼠胃排空、肠推进的影响及对家兔离体回肠平滑肌肌条收缩活动的影响。方法将48只昆明小鼠随机分为陈皮提取物高剂量(10mL/kg)、中剂量(5mL/kg)、低剂量(2.5mL/kg)及空白对照(蒸馏水)4个组,每组12只,以胃肠内标记物墨汁在小鼠胃内色素相对残留率及肠推进比为指标,观察提取物对在体小鼠胃排空及肠推进的影响;在离体条件下,以家兔回肠平滑肌肌条的张力和频率为指标,通过不同浓度的陈皮提取物作用于家兔离体回肠平滑肌,观察提取物对离体回肠平滑肌收缩活动的影响。结果陈皮提取物在2.5、5、10mL/kg剂量下都能够提高小鼠胃排空率和促进小肠的推进作用,与对照组相比,差异具有显著性;在离体条件下,陈皮提取物在0.1g/L时能显著降低家兔离体回肠平滑肌的张力和收缩频率;在0.05g/L时也可明显降低平滑肌张力;在0.025g/L时,家兔离体回肠平滑肌的张力、收缩频率无显著性变化。结论陈皮提取物对在体动物胃肠动力具有促进作用,并存在一定的剂量—效应关系,而对离体肠管有一定的抑制作用,其抑制效应可能由胆碱能M受体介导。  相似文献   

4.
滇虹妇康膏对离体及在体大鼠子宫调节作用的研究   总被引:1,自引:0,他引:1  
目的 :实验观察滇虹妇康膏对子宫收缩的影响 .方法 :采用文献报道的大鼠离体和在体子宫实验方法 .结果 :滇虹妇康膏体外 (1 5 ,3 0mg生药 /mL)显著增强大鼠离体子宫最大收缩幅度 (P <0 0 5 ) ,显著降低基础张力 (P <0 0 1) ,并在基础张力及最大收缩幅度方面显著对抗垂体后叶激素作用 (P <0 0 5 ) ;滇虹妇康膏 (13 3,2 6 6g生药 /kg)对在体大鼠子宫收缩频率呈双向调节作用 ,同时在收缩频率及收缩幅度方面显著对抗垂体后叶激素作用 (P <0 0 5 ) .结论 :滇虹妇康膏具有调节子宫收缩的作用  相似文献   

5.
目的 观察泽泻醇B( 2 3-acetatealisolB)对豚鼠离体回肠收缩的影响。方法 将豚鼠离体回肠悬吊于麦氏浴槽中 ,通过体外给药的方法 ,观察回肠的收缩情况 ,并计算回肠的收缩幅度。结果 浴槽浓度为 1 .2 5× 1 0 -2 mg/mL、1 .2 5×1 0 -3 mg/mL、1 .2 5× 1 0 -4 mg/mL时 ,泽泻醇B对组胺引起的豚鼠离体回肠收缩有对抗作用 ,且随着剂量的增加而增加。结论 泽泻醇B拮抗组胺对离体豚鼠回肠的收缩作用是通过非特异性竞争而发挥的。  相似文献   

6.
清香桂提取物对胃肠功能影响的实验研究   总被引:4,自引:0,他引:4  
目的:评价清香桂提取成分对胃肠道平滑肌的作用和对急性胃溃疡的防治作用.方法:采用豚鼠离体回肠、大鼠离体胃底条和十二指肠实验,活性碳法观察对小鼠的肠推进作用,应用盐酸-乙醇致小鼠实验性胃溃疡模型,观察清香桂正丁醇部分和总碱对胃肠道平滑肌收缩功能和对急性胃溃疡的防治作用.结果:清香桂正丁醇部分和总碱对乙酰胆碱和组胺引起的豚鼠离体回肠平滑肌强烈收缩无明显抑制作用,而药物本身可引起回肠平滑肌收缩,并呈浓度依赖性,EC50分别为9.9×10-6和7.3×10-6g/L;对离体大鼠胃肌条亦有明显的收缩作用,其EC50分别为1.65×10-5和4.3×10-6g/L;此外,清香桂总碱尚能增加十二指肠平滑肌的自发收缩幅度,对收缩频率无明显影响;而正丁醇提取物对十二指肠平滑肌的自发收缩幅度和频率均无明显影响.清香桂正丁醇部分和总碱均明显促进小鼠肠蠕动,而对盐酸-乙醇致小鼠实验性胃溃疡无明显的保护作用.结论:提示清香桂提取成分对胃肠道平滑肌有明显的兴奋作用,促进胃肠蠕动,所含甾体生物碱可能是其主要的活性成分.  相似文献   

7.
Nisoldipine 10μg/kg明显缩小家兔心肌梗塞范围,3μg/kg明显降低麻醉狗血压及左室内压,减慢心率,增加冠脉血流量及冠状窦血氧含量;1,3,10μg/kg降低麻醉狗冠脉及椎动脉阻力强于股动脉阻力。1~10μg明显增加离体豚鼠心脏冠脉沉量,抑制心肌收缩振幅,大剂量则减慢心率。2.6×10~(-10)~1×10~(-8)M非竞争性对抗KCl收缩家兔离体主动脉条反应,但不影响去甲肾上腺素所致收缩反应。nisoldipine抑制Cacl_2所致离体兔耳血管收缩反应的IC_(50)为1.7×10~(-11)M,而抑制去甲肾上腺素收缩反应的JC_(50)为4.0×10~(-4)M。小鼠灌胃LD_(50)为292±32mg/kg。  相似文献   

8.
目的:研究白萝卜提取物(Raphanus sativus extract,Ecr)对在体小鼠胃排空及肠推进的影响及对家兔离体回肠平滑肌条收缩活动的影响.方法:通过水煮蒸发的方法,制备得到白萝卜提取物;将48只昆明小鼠随机分为白萝卜提取物高剂量(20 mL/kg)、中剂量(10 mL/kg)、低剂量(5 mL/kg)组及...  相似文献   

9.
妇血宁3mg/ml有兴奋大鼠离体子宫平滑肌作用,生物效价测定表明1mg妇血宁收缩大鼠和小鼠子宫肌的作用强度与5×10~(-5)u和1.8×10~(-5)u的垂体后叶素相当。2mg/ml妇血宁松弛豚鼠气管条和家兔回肠平滑肌,对蟾蜍心脏有兴奋作用。猫静脉注射妇血宁500mg/kg血压先升后降,心率加快,未见其致敏作用。  相似文献   

10.
鸡矢藤[Paederia Scandens]提取物0.32mg 加入营养槽内,对小白鼠各期离体子宫的收缩张力、收缩强度、收缩频率以及子宫活动力均有增强作用。与给药前比较,诱导期组分别增加85%、47%、21%和72%;动情期组分别增加70%、77%、41%和41%;非动情组分别增加47%、76%、62%和66%,而且三组间作用无显著性差异。本品还能增强 PGE_2对小白鼠离体子宫的收缩张力、收缩强度、收缩频率以及子宫活动力。与 PGE_2的作用比较,分别增加34%、39%、9%和45%,但收缩频率增加无统计学意义(P>0.05)。上述结果表明,鸡矢藤提取物对小鼠子宫有显著性兴奋作用,而且还能增强PGE_2对小鼠子宫兴奋作用,其机制尚待进一步研究。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

20.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

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