AUTHOR INDEX FOR VOLUME 5

A AGARWAL, A.K., 87 B BANERJEE, T.K., 325 BHATTACHARYA, B., 178 BHATTACHARYA, SHELLEY, 18, 178 236, 355 BIAS, HVARONG, 115 BOSE, SHAMBHUNATH, 355 C CAI, LU, 46 CAI, SHIWEN,130 CAO, ZHAOJING, 136 CHANG, LOUIS, W., 283, 293 CHAUDHURY, SHIBANI, 355 CHEN, CHANG, 149 CHEN, CHIEH-Fu, 283,293 CHEN, HUA, 229 GUAN, LI, 257 GUHA, DHRITI, 92 GUPTA, B.N., 321 GUPTA, N., 87 H HALDER, P., 178 HE, SHUNZHONG, 115 HE, XIWEN, 276 HOWELL, KATHY, S., 3 HU, WENJUAN, 161 HUANG, CHENGYU, 25 HUANG, JIANG, 136 HUANG, JIANPING, 115 HUSAIN, FIDA, 251 J JIANG, XUEZHI,266 JING, XIPENG, 221 JIU, YIQUAN, 115 JONES, ROSS, E., 314 LU, JING, 276 LUO, XUEYUN, 161 LUO, YUNDA 366 M MALLICK, NIRUPAMA, 65, 241 MAO, XU, 125 MATHUR, A.K., 321 MUKHERJEE, DILIP, 92 N NARA NG, S., 321 NASU, M., 25 OKUDA, A., 25 PAK, D.C.H., 99     

延边大学医学学报编辑委员会

主　任 :孙东植副主任 :崔炯谟 ,金　海 ,金哲洙委员 :(按姓氏笔划排列 )　　　　方今女 ,皮永浩 ,朴伍范 ,朴惠善 ,朴熙绪 ,全哲山 ,安昌善 ,许文燮 ,许东哲 ,李林虎 ,宋京郁 ,吴　光 ,吴龙仁 ,金正勇 ,金东洙 ,金青松 ,金顺吉 ,金哲虎 ,孟繁平 ,柳明洙 ,崔京浩 ,韩春姬延边大学医学学报编辑委员会     

Subject Index Chinese Medical Journal Volume 117,2004

abortion,spontaneous,492absorptionratecoefficient,689Acat,1259acetylcholinetest,1564acetylcholine,1161actinskeleton,213actionpotentials,532,538activationmarkers,347activation,1665activinreceptorlikekinase1gene,808acupunctureandmoxibustion,1502acutecoronarysyndrome,133,1130acutelunginjury,1859acutemyocardialinfarctin,492,1443,1772acutepromeylocyticleukaemia,953acuterejection,408adenoassociatedvirus,1370adenocarcinoma,1590adenoidectomy,657adenosinetriphosphate,581adenoviralvector,1353adenovirusty…     

浅谈眼针的护理

1 眼针适应症 眼针对中风偏瘫早期,急性扭伤,落枕,胆绞痛,心律不齐,膈肌痉挛,三叉神经痛,坐骨神经痛,胃痉挛,鼻炎,牙痛,面瘫,五十肩,腰痛,呕吐,腹泻,便秘,痢疾,足跟痛,神经衰弱,月经不调,阳痿,肾绞痛,心绞痛,高血压病,电光性眼炎等均有较好疗效。2 眼针禁忌症 病势垂危、抢救期间、精神错乱、气血虚脱者     

AUTHOR INDEX FOR VOLUME 18

A AN,Lx一J认,297 AN,S哑一JU胡,302 DU,CHAN,326 DU,Ho喝,227 DU,R溯一LEI,363 DU,X川一Hu^,181 HUSA】N,K人ZIM,198 B BAO,YONG叼M则G,297 BAO,YU一QIAN,103 BIAN,Ll一Hu^,192 BIAN,QIAN,108 B认N,ZHI三No一ZH州。,87 FAN,HoNG一X泥,36 FENG,YE一C甩瀚,5 FU,CHA。一WEI,l Fll,Q水G一丫人N,159 FU,ZH洲G一Guo,326 C G CAI,Y人N一凡x,341 CHAO,FU一HUAN,43,164 CHE,F它Ne一X认NG,82 CHEN,B从。,260 CHEN,BIN,260 CHEN,BING…     

甘草(Licorice)

又名Alcacua,Alcazuz,Chinese Licorice,Gan Cao,GanZao,Glycyrrhiza,Lakritze,Licorice Root,Liquiritiaeradix,Liquirizia,Liquorice,Orozuz,Phytoestrogen,Reglisse,Regliz,Russian Licorice,Spanish Licorice,Sub-holz,Sweet Root。植物名Glycyrrhiza glabra;G.glabra typi     

养生三字经

老年人,是个宝,喜谦让,免争吵,养生法,应知道,情绪稳,睡眠好。 退休后,勿气馁,失意时,玩花鸟,交游广,阅历深,听音乐,看舞蹈。 尝酸甜,品苦乐,想得开,乐逍遥,     

AUTHOR INDEX FOR VOLUME 16

AG,J气,︸IJ内,︶飞︸内」AN,Yl一HU^,90,2 12ANGELOS,GEORGE,AN」U,B”276BGAO,Z川一X创q 90GE,KE一You,348GU,SHLI一ZHU,83GUO,CllANG一J认NqGUPTA,K获lsH卜A,9GIJ冈叭,R,369BALL,M月〕ELEINE兀BAO,YtJ一QtJ胡,206Bl,SHENG一Ll,246HBYRD,DANIELM,355CCAI,DoNq 53CAI,N认N一SHE卜q 267CAO,W曰,157CHAKRABARTI,工,68CHANq HAo,83CHEN,BAo一S现Nq 227CHEN,B创G一HENQ 133CHEN,CHIJN一MINq 187,195CHEN,HUA一L班,163CHEN,HUAx一HONq 53,295CHEN,LEI,206CHEN…     

仙家禁疮法

李野朴,古代气功家. 主治:头、面、肩、背处各种疮疾.具体操作法:以身坐定,直舒两脚,两手掌按住大腿根,调息运气,以意引导攻冶疾处,共十二次。配合炼功,可服“羌活白芷汤”.药方组成:柴胡,茯苓,防风,荆芥,黄连,泽泻,当归,白术,蔓荆,石膏,苍术,辛夷,生地,川芎,藁本,甘草,白芷,羌活,黄芩,细辛,芍药.以上二十一味各等分,加生姜二,三片,用水煎服,每日一杯.     

延边大学医学学报编辑委员会

主　任 :孙东植副主任 :崔炯谟 ,金　海 ,金哲洙委员 :(按姓氏笔划排列 )　　　　方今女 ,皮永浩 ,朴惠善 ,朴熙绪 ,全哲山 ,安昌善 ,许文燮 ,许东哲 ,李林虎 ,宋京郁 ,吴　光 ,吴龙仁 ,金　政 ,金正勇 ,金东洙 ,金青松 ,金顺吉 ,金哲虎 ,孟繁平 ,柳明洙 ,南极星 ,韩春姬延边大学医学学报编辑部主　　编 :金哲洙责任编辑 :李美花延边大学医学学报编辑委员会     

Effect of Smac on TRAIL-induced apoptosis of prostate cancer cell line PC-3 and the molecular mechanism

The effect of Smac gene on the TRAIL-induced apoptosis of the prostate cancer cell line PC-3 and the molecular mechanism were investigated. The Smac gene was transfected into PC-3 cells under the induction of liposome. The intrinsic Smac gene expression was detected by Western blotting. After treatment with TRAIL as an apoptosis inducer, in vitro cell growth activity was as-sayed by MTT colorimetry. The apoptosis rate of PC-3 cells was determined by annexin Ⅴ-FITC and propidium iodide staining flow cytometry. The expression of cellular XIAP and caspase-3 genes was examined by Western blotting. Smac-transfected cells (PC-3/Smac group) had significantly in-creased Smac protein level as compared with PC-3 controls (P<0.01). After induction with 100-200 ng/mL TRAIL for 12-36 h, cellular proliferation rate in PC-3/Smac group was significantly lower than in PC-3 controls (P<0.05). After induction with 100 ng/mL TRAIL for 24 h, the apoptosis rate in PC-3/Smac group was significantly enhanced as compared with that of PC-3 controls (P<0.05). Ac-cordingly, the XIAP expression level was down-regulated significantly (P<0.05) and caspase-3 sub-unit P20 was up-regulated significantly (P<0.05). It is suggested that the over-expression of cellular Smac can inhibit inhibitor of apoptosis proteins (IAPs), enhance caspases activity and the apoptosis rate of PC-3 cells induced by TRAIL, which may provide a useful experimental basis for prostate cancer therapy.     

Effects of blocking androgen receptor expression with specific hammerhead ribozyme on in vitro growth of prostate cancer cell line

Aseriesofstudieshaveindicatedthatandrogenreceptor(AR) playsakeyroleinthedevelopmentofprostatecancerbymediatingandrogenactivityontargetcells 1Ithasbeen proposedthatdecreasingandrogenandARlevelscouldbeaneffectivetherapeuticstrategyforprostatecancer 2 　InthisstudyaribozymeapproachtoselectivedegradationofARmRNAwasusedtoexploretheeffectsofblockingARexpressiononinvitrogrowthofhumanprostatecancercells METHODSRibozymedesignandsynthesisUsingtheMFOLDcomputerprogramanARspecificribozyme(RZ)was…     

Effect of small interfering RNA targeting survivin gene on biological behaviour of bladder cancer

Background Bladder cancer is the most common type of urinary system tumours. It is frequently associated with genetic mutations that deregulate the cell cycle and render these tumours resistant to apoptosis. Survivin, a newly discovered member inhibitor of apoptosis protein （IAP） family in several human cancers, by inducing cell proliferation and inhibiting apoptosis is frequently activated in bladder cancer. We studied the influence of small interfering RNA （siRNA） targeting survivin on the biological behaviour of bladder cancer cells. Methods A double strand survivin target sequence specific siRNA was designed and synthesized. After transfection of bladder cancer cell line T24 by siRNA/liposome complex with increasing concentrations （50-200 nmol/L）, the transfectant cells were intratumourally injected at different doses （5 μg or 50 μg）. The effects were measured in vitro and in vivo. Results The selected siRNA efficiently down-regulated survivin mRNA expression in a dose and time dependent manner. The maximal effect was achieved at the concentration of 100 nmol/L, at which survivin expression level was down-regulated by 75.91%. The inhibition rate of cell growth was 55.29% （P〈0.01） and the markedly increased apoptotic rate was 45.70% （P〈0.01）. In vivo intratumoural injection of 50 μg siRNA-survivin could notably orevent the growth of bladder cancer （P〈0.01） in xenografted animals. Conclusion The application of siRNA-survivin could markedly inhibit survivin expression in bladder cancer cell line by inducing apoptosis and inhibiting the growth of the tumour. It may become a new gene therapy tool for bladder cancer.     

Inhibitory effect of tea polyphenols on renal cell apoptosis in rat test subjects suffering from cyclosporine-induced chronic nephrotoxicity

Objective To investigate the inhibitory effect of tea polyphenols on renal cell apoptosis in rat test subjects suffering from cyclosporine A (CsA)-induced chronic nephrotoxicity.Methods Four groups of rats with CsA-induced chronic nephrotoxicity were respectively treated with vehicle olive oil, tea polyphenols, CsA and tea polyphenols plus CsA. At the end of the 28th day of treatment, 24 hours urine and blood samples were obtained, and the animals were then sacrificed. The serum and urine samples were analysed for creatinine clearance, and kidney tissue was used for pathologic analysis of renal tubular injury and interstitial fibrosis. The TUNEL assay, apoptosis-related enzyme caspase-3 mRNA detected by RT-PCR, and its enzymatic activity were analysed for the possible detections of cell apoptosis.Results CsA-treated rats displayed increased apoptosis of the tubular and interstitial cells, in comparison with vehicle-treated controls (18. 3±4. 6 vs 4. 8±1.3 cells/mm2, P < 0. 05 ) . In comparision with a     

Casticin对宫颈癌细胞凋亡和线粒体凋亡途径的影响

目的探讨紫花牡荆素（Casticin,CAS）是否通过线粒体凋亡途径诱导人宫颈癌HeLa细胞凋亡。方法体外培养人宫颈癌HeLa细胞。EusA法检测细胞组蛋白／DNA碎片;碘化丙啶（propidium iodide,PI）染色流式细胞术（flow cytometry,FCM）分析细胞凋亡率;比色法测定细胞半胱天冬酶caspase-9,-3活性;罗丹明123探针FCM检测细胞线粒体跨膜电位。结果CAS以浓度依赖方式增高HeLa细胞组蛋白／DNA碎片水平和凋亡率（P〈0．05）。CAS增强HeLa细胞caspase-9,-3活性（P〈0．05）和诱导Hela细胞线粒体膜电位下降（P〈0．05）,呈浓度依赖性。结论CAS诱导HeLa细胞凋亡涉及线粒体凋亡途径的激活。     

三重靶向介导的Smac过表达对乳腺癌MDA-MB-231细胞凋亡和周期进程的影响

目的：利用基因重组技术获得三重靶向性Smac过表达的条件复制型腺病毒,探讨其对乳腺癌MDA-MB-231细胞凋亡和周期的影响。方法：利用重组技术构建Smac过表达载体pShuttle-Egr1-Smac-HRE-hTERT-E1A-E1Bp-E1B55K,与骨架载体pAdEasy在BJ5183（AdEasy-1+）菌中进行重组,获得Smac过表达的条件复制型腺病毒CRAd.pE-Smac,感染MDA-MB-231细胞后,利用化学试剂氯化钴模拟肿瘤细胞乏氧状态,设立对照组、CARd.pE-Smac组、乏氧组和CARd.pE-Smac+乏氧组,并根据是否进行4 Gy照射,每组分为未照射组和照射组,共8个实验组。利用Western blotting法检测各组细胞Smac蛋白的表达,利用流式细胞术检测细胞凋亡率和不同周期进程细胞百分率。结果：Western blotting法检测,经条件复制型腺病毒CRAd.pE-Smac感染、乏氧和照射后,Smac蛋白表达水平增加,CARd.pE-Smac+乏氧+4Gy组Smac蛋白表达水平最高。流式细胞术检测,与对照组比较,CARd.pE-Smac组、乏氧组和CARd.pE-Smac+乏氧组细胞凋亡率均明显升高（P<0.05或P<0.01）;与相应未照射组比较,4 Gy照射后CARd.pE-Smac+4Gy组、乏氧+4Gy组和CARd.pE-Smac+乏氧+4Gy组细胞凋亡率均明显升高（P<0.05或P<0.01）,CARd.pE-Smac+乏氧+4Gy组升高最为明显,且S期和G₂/M期细胞百分率明显增加（P<0.05或P<0.01）,与诱导凋亡的结果有相似的趋势。结论：在条件复制型腺病毒CRAd.pE-Smac感染MDA-MB-231细胞、并经乏氧和辐射处理后,实现了三重靶向介导的Smac过表达,其具有促进肿瘤细胞凋亡和诱导G₂/M期细胞阻滞的作用。     

Smac/DIABLO Promotes Mitomycin C-induced Apoptosis of Bladder Cancer T24 Cells

Thesecondmitochondria derivedactivatorofcaspase(Smac)ordirectIAPbindingproteinwithlowpl(DIABLO),amitochondrialproteinthatisreleasedtogetherwithcytochromeCfromthemi tochondriainresponsetoapoptoticstimuli,wasrecentlyfoundtopromotecaspaseactivationbybindingandneutralizingtheinhibitorofapoptosisproteins(IAPs)[1-3].Inthisstudy,inordertoex plorethepossibilityofSmac/DIABLOasanoveltargetofgenetherapyforbladdercancer,theeffectoftheSmacexpressiononthemitomycinC(MMC)inducedapoptosisofthebladder…     

汉防己甲素体外辅助多柔比星杀伤胃癌细胞及机制研究

目的探讨中药活性成分汉防己甲素是否能在体外辅助多柔比星杀伤胃癌细胞并研究其机制。方法噻唑蓝(MTT)法检测胃癌细胞系MGC-803相对细胞活力。Western blot实验检测MGC-803细胞中小窝蛋白-1(caveolin-1)表达水平、蛋白激酶B(AKT)和B淋巴细胞瘤-2蛋白(Bcl-2)相关细胞死亡激动子(Bad)磷酸化水平、半胱天冬酶-9(caspase-9)和半胱天冬酶-3(caspase-3)活化水平。免疫共沉淀法检测Bad与大分子B淋巴细胞瘤蛋白(Bcl-xl)及Bcl-2相互作用。流式细胞术检测MGC-803细胞的凋亡。结果汉防己甲素+多柔比星组MGC-803相对细胞活力(0.34±0.03)显著低于多柔比星(0.79±0.06)(P0.05),汉防己甲素+多柔比星组MGC-803的细胞凋亡率显著高于多柔比星组[(40.32±3.51)%比(12.23±1.13)%,P0.05]。汉防己甲素+多柔比星组MGC-803细胞的caveolin-1表达水平、AKT和Bad的磷酸化水平均低于多柔比星组,而汉防己甲素+多柔比星组MGC-803细胞的Bad与Bcl-xl及Bcl-2结合水平、caspase-9和caspase-3活化水平均高于多柔比星组。汉防己甲素+多柔比星+caveolin-1质粒组MGC-803相对细胞活力显著高于汉防己甲素+多柔比星组[(0.71±0.06)比(0.34±0.03),P0.05],而其细胞凋亡率显著低于汉防己甲素+多柔比星组[(15.94±1.22)%比(40.32±3.51)%,P0.05]。结论汉防己甲素可能通过caveolin-1/AKT/Bad途径增强多柔比星对胃癌细胞的凋亡诱导活性。     

Par-4基因沉默对人骨髓间充质干细胞中Smac基因表达和半胱氨酸蛋白水解酶3酶活性的影响及其抗凋亡作用

目的 研究Par-4基因沉默对人骨髓间充质干细胞(hBMSC)中Smac基因表达和半胱氨酸蛋白水解酶(Caspase)3酶活性的影响及其抗凋亡作用.方法 原代培养hBMSC.谷氨酸诱导hBMSC凋亡.设计和合成两对针对Par-4基因mRNA的小RNA干扰(siRNA)序列(Par-4-SiRNA-1、2),构建真核细胞表达载体,用脂质体介导转染hBMSC,利用G418筛选稳定表达细胞株.实时荧光定量PCR法检测Par-4 mRNA表达水平.流式细胞术测定细胞凋亡百分率.Western印迹测定Smac蛋白表达量.比色法测定Caspase-3酶的活性.结果 设计的Par-4-SiRNA-1、2可显著诱导hBMSC中Par-4基因沉默,Par-4 mRNA水平分别被降低88%、67%,谷氨酸诱导hBMSC凋亡,凋亡百分率为(58.9±8.9)%.Par-4-SiRNA-1可显著拮抗谷氨酸的诱导凋亡作用,凋亡百分率降为(37.2±6.3)%(F=58.26,P＜0.01).Par-4-SiRNA-1对谷氨酸诱导的hBMSC中Smac蛋白表达上调(P＜0.01)和Caspase-3酶活性上调(P＜0.01)有显著的抑制作用(P＜0.01).结论 Par-4基因沉默能拮抗谷氰酸对hBMSC凋亡的诱导作用.其机制可能与Smac基因表达和Caspase-3酶活性被抑制有关.