五味子乙素诱导的HSP27和HSP70对小鼠肝损伤的保护作用

目的 观察五味子乙素(Sch B)诱导的HSP27和HSP70对小鼠肝损伤的保护作用.方法 采用扑热息痛(APAP)小鼠肝损伤模型,Sch B和槲皮素给药后检测血清转氨酶水平,Western blot法检测小鼠肝脏HSP27和HSP70的表达.结果 Sch B在APAP模型小鼠显著诱导HSP27和HSP70的表达及降低小鼠血清转氨酶水平,槲皮素可抑制上述作用.结论 Sch B可通过诱导小鼠肝脏HSP27和HSP70的表达而发挥保肝作用.     

五味子乙素对小鼠肝脏HSP27和HSP70的诱导作用

目的 观察五味子乙素对正常小鼠肝脏HSP27和HSP70的诱导作用.方法 将实验小鼠分为空白对照组、五味子乙素给药组及高温处理组,采用Western blot法检测小鼠肝脏HSP27和HSP70的表达.结果 五味子乙素200mg/kg、100mg/kg、50mg/kg组小鼠肝脏的HSP27和HSP70表达均明显升高,但远远小于热休克反应对其表达的诱导.结论 五味子乙素能诱导小鼠肝脏HSP27和HSP70的表达.     

加味四逆汤对Con A肝损伤小鼠细胞凋亡的保护作用

目的探讨加味四逆汤对刀豆蛋白A（Con A）诱导肝细胞损伤过程中肝脏组织Fas抗原的表达和血清肿瘤坏死因子-α（TNF-α）水平的干预情况。方法雌雄各半的NIH小鼠随机分成5组：正常对照组、模型组、阳性药物组、加味四逆汤大、小剂量组,采用Con A（18mg·kg-1）尾静脉注射制作急性免疫性肝损伤模型。造模给药结束后,观察血清中TNF-α含量变化及肝组织细胞Fas抗原表达。结果模型组小鼠肝脏细胞内有大量的Fas抗原表达,且血清中TNF-α含量也明显升高,与对照组比较差异具有统计学意义（P<0.05）;对照组和药物组血清中TNF-α水平变化不大,肝细胞Fas抗原表达不明显。结论加味四逆汤能够干预Con A小鼠肝脏组织Fas抗原和血清TNF-α水平的表达,对急性肝损伤有较好的保护作用。     

弓形虫热休克蛋白70诱导建立弓形虫感染小鼠急性肝损伤模型研究

[目的]通过给弓形虫感染小鼠腹腔注射给予弓形虫热休克蛋白70(T.g.HSP70)建立急性肝损伤模型,观察T.g.HSP70诱导弓形虫感染小鼠急性肝损伤的作用机制.[方法]将20只KM雌性小鼠随机分为正常对照组、感染组、T.g.HSP70组及模型组(感染+T.g.HSP70).于弓形虫感染第7d,腹腔注射给予小鼠T.g.HSP70.次日处死小鼠,取血液,分离血清,取肝脏组织.测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)水平,测定肝组织中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量.[结果]模型组小鼠ALT,AST,MDA水平较其他组明显升高(P<0.01),SOD水平明显降低(P<0.05).[结论]T.g.HSP70可诱导弓形虫感染小鼠急性肝损伤,其机制可能与T.g.HSP70加重弓形虫感染小鼠肝组织的脂质过氧化有关.     

HSP70在对乙酰氨基酚诱导的小鼠急性肝损伤组织中的表达

目的研究(热体克蛋白70)HSP70在对乙酰氨基酚诱导的小鼠急性肝损伤中的动态变化,进一步阐明HSP70在药物性肝损伤中的重要作用。方法健康雄性小鼠40只,分别腹腔注射对乙酰氨基酚溶液(用生理盐水稀释,550 mg/kg),在注射对乙酰氨基酚后62,44,2,54 h分别眼球取血,分离血清检测AST和ALT活性,同时取小鼠肝脏制备10%肝匀浆,每个时间点10只小鼠。同时取10只正常小鼠的血清和肝脏作为对照进行研究。利用蛋白印迹方法检测小鼠急性肝损伤过程中HSP70的表达变化。结果小鼠注射对乙酰氨基酚后,血清中AST和ALT活性随着时间的延长,酶活性逐渐升高,24 h达到高峰,54 h逐渐恢复到正常水平。HSP70在注射对乙酰氨基酚后6 h,其表达量略有增高,在24 h表达量达到高峰,说明小鼠肝脏的应激反应达到了高峰,此后HSP70的表达量逐渐恢复到正常水平。结论 HSP70在对乙酰氨基酚诱导的小鼠急性肝损伤过程中表达出现显著变化,可能在肝损伤的修复和再生过程中起到重要的作用。     

白介素22融合蛋白对刀豆蛋白A诱导肝损伤病理状态下肝切除后肝再生的影响

目的 研究白介素22融合蛋白(IL-22-FP)对刀豆蛋白A(ConA)诱导急性肝损伤后行70%肝切除手术(PHx)的小鼠术后肝再生及肝脏保护的作用.方法 建立小鼠ConA肝损伤和PHx手术模型,术后32 h通过过量麻醉法处死小鼠.5-溴脱氧尿嘧啶核苷染色,观察小鼠肝脏再生情况;全自动生化分析仪检测小鼠血清谷丙转氨酶(ALT)和谷草转氨酶(AST)水平;HE染色观察小鼠肝脏组织损伤情况;Western blot法检测小鼠肝组织中增值细胞核抗原(PCNA)、细胞周期蛋白D1(CycliD1)、信号转导子及转录激活子3(STAT3)及p-STAT3蛋白的表达情况.结果 ConA+PHx+IL-22-FP组小鼠同ConA+PHx+rh-IL-22组及其他组小鼠相比,表现出更多的肝细胞增殖和更高的肝指数(肝脏重量/体重);HE染色显示ConA+PHx+IL-22-FP组小鼠肝脏坏死及炎症细胞浸润程度较低;生化检测显示ConA+PHx+IL-22-FP组血清ALT和AST水平明显低于其他组;Western blot结果表明ConA+PHx+IL-22-FP组小鼠的肝脏组织PCNA、CyclinD1和p-STAT3蛋白的表达量也明显高于其他组.结论 IL-22-FP能够促进由ConA诱导肝损伤的小鼠PHx后的肝再生,并表现出显著的肝脏保护作用.     

芹菜素对刀豆蛋白A诱导的免疫性肝损伤小鼠的保护作用

目的 研究芹菜素(AP)对免疫性肝损伤小鼠的保护作用.方法 通过尾静脉注射20 mg/kg刀豆蛋白A(Con A) 诱发免疫性肝损伤小鼠模型,连续6天给予小鼠75、150和300 mg/kg AP灌胃,用石蜡切片-HE染色检测肝脏病理变化,速率法检测谷丙转氨酶(ALT) 的活性.结果 与模型组比较,75～300 mg/kg AP 均能抑制Con A 诱发的肝门静脉炎症细胞侵润和血清ALT 活性的升高,具有浓度依赖性.结论 AP 对Con A 诱发的小鼠免疫性肝损伤具有较好的保护作用.     

双歧杆菌脂磷壁酸对刀豆蛋白A诱导小鼠免疫性肝损伤的保护作用

目的 研究双歧杆菌脂磷壁酸(LTA)对ConA诱导的小鼠免疫性肝损伤的保护作用,探讨其在该疾病预防和治疗中的应用可行性.方法 小鼠随机分为正常组、模型组、联苯双酯组(150 mg/kg)和LTA处理组(100、200和400μg/kg),尾静脉注射Con A复制小鼠免疫性肝损伤模型；观察各组小鼠的一般情况及肝、脾指数,生化法和酶联免疫法测定血清ALT、AST和TNF-α的含量；RT-PCR检测小鼠肝组织内NF-кB的基因表达.结果 LTA处理组小鼠的一般情况和肝脏指数均好于模型组,其血清内ALT、AST和TNF-α的水平显著低于模型组(P＜0.05),但与联苯双酯组基本相当(P＞0.05)；不同浓度LAT处理组小鼠肝细胞NF-кB的mRNA水平均低于模型组(P＜0.05),且LTA 400μg/kg组与联苯双酯组基本相当(P＞0.05).结论 双歧杆菌脂磷壁酸的双向免疫调节作用可以有效保护Con A诱导的小鼠免疫性肝损伤.     

肝康颗粒对Con A诱导小鼠急性免疫性肝损伤的Caspase-3mRNA表达的影响

目的:观察caspase-3基因在Con A性肝损伤小鼠肝组织中的表达、肝细胞凋亡的情况以及肝康颗粒对其影响.方法:TUNEL法肝细胞凋亡,用逆转录-聚合酶链反应(RT-PCR)半定量分析肝组织Caspase-3 mRNA表达水平.结果:模型组肝组织中Caspase-3 mRNA的相对单位值最高,说明在模型组动物肝组织Caspase-3 mRNA有明显表达;肝康颗粒低、中、高剂量组小鼠Caspase-3 mRNA的相对单位值均低于模型组.表示肝康颗粒对Con A诱导肝损伤小鼠抑制肝细胞凋亡作用.结论:肝康颗粒能下调或延迟肝细胞Caspase-3 mRNA的表达,影响蛋白酶级联切割过程,进而减轻或抑制Con A肝损伤小鼠肝细胞凋亡.     

重组鲨肝刺激物质类似物对刀豆蛋白A致小鼠急性肝损伤的保护作用

为探讨重组鲨肝刺激物质类似物(r-sHSA)对刀豆蛋白A（Con A）致小鼠急性肝损伤的保护作用及其作用机制,利用尾静脉注射Con A制备小鼠急性肝损伤动物模型,以谷丙转氨酶（ALT）和谷草转氨酶（AST）活性以及组织病理变化为指标判断r-sHSA(30,60,120 μg/kg)对免疫性肝损伤的保护作用;同时,检测肝组织丙二醛、谷胱甘肽水平和总抗氧化能力以及血清中TNF-α和IFN-γ的含量,并测定肝组织匀浆中iNOS活力。结果表明,3个给药剂量的r-sHSA均可显著降低肝损伤小鼠血清转氨酶活性,明显减轻肝细胞坏死,减少炎细胞浸润,改善肝脏氧化应激水平,并下调炎症因子表达。结果提示,r-sHSA对Con A致小鼠急性肝损伤具有明显的保护作用,其机制可能与抗氧化和抗炎作用有关。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…