白藜芦醇酒剂对老龄小鼠MDA、SOD和GSH-Px的影响

目的探讨白藜芦醇酒剂对老龄小鼠丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）活力的影响。方法将60只昆明种老龄小鼠按4%溶血液MDA水平随机分为空白对照组,酒基对照组,白藜芦醇酒剂低、中、高剂量组,分别连续给药30d后,分别测定MDA含量、SOD和GSH-PX活力。结果与酒基对照组比较,高剂量组能显著降低血中MDA含量,差异具有统计学意义（P〈0.05）;能显著提高血清SOD活力,差异具有统计学意义（P〈0.05）;各剂量组对血清GSH-PX活力无明显影响（P〉0.05）。结论白藜芦醇酒剂具有降低血液MDA含量,提高血清SOD活力的作用。     

苦瓜醇提取物降血糖作用动物实验研究

目的观察苦瓜醇提取物对II型糖尿病小鼠的降血糖作用。方法采用高脂饲料喂饲断乳小鼠制备II型糖尿病模型,选血糖≥8mmol/L者分入对照组和2个剂量组。剂量组分别给予0.125g/kg·bw、0.500g/kg·bw剂量的苦瓜醇提取物。30d后测定小鼠空腹血糖、糖耐量、血生化指标和血清MDA含量、SOD活力。同步给予正常小鼠0.500g/kg·bw剂量的苦瓜醇提取物观察其对正常小鼠血糖的影响。结果与高血糖模型小鼠对照组相比,苦瓜醇提取物0.500g/kg·bw剂量组空腹血糖显著降低(P0.05),实验前后血糖下降百分率显著提高(P0.01),糖耐量显著改善(P0.05)。0.125g/kg.bw、0.500g/kg.bw剂量组高血糖小鼠血清谷丙转氨酶显著降低(P0.05),0.500g/kg.bw剂量组血清白蛋白、尿素氮显著降低(P0.01)。各剂量对高血糖小鼠的其它生化指标、血清MDA含量、SOD活力无显著影响。苦瓜醇提取物对正常小鼠的血糖无显著影响。结论苦瓜醇提取物可降低II型糖尿病小鼠的高血糖,改善糖耐量,但可导致血清白蛋白降低。临床上应用时应加注意。     

不同剂量茶多酚对小鼠血清SOD、MDA水平影响的研究

目的观察不同剂量茶多酚（TP）对小鼠血清SOD、MDA水平的影响。方法将小鼠按体重随机分为四组,每组30只,雌雄各半。各组小鼠分别给予不同剂量茶多酚的饲养处理：1）对照组：普通饲料;2）实验组Ⅰ：TP5mg/kg.bw;3）实验组Ⅱ：TP15mg/kg.bw;4）实验组Ⅲ：TP25mg/kg.bw。于90天时测定小鼠血清SOD、MDA水平。结果与对照组相比,实验组Ⅰ小鼠血清SOD活性增强（P〈0.05）,MDA含量、SOD/MDA变化不显著（P〉0.05）;实验组ⅡSOD活性增强（P〈0.01）,MDA含量变化不显著（P〉0.05）,SOD/MDA升高（P〈0.05）;实验组ⅢSOD活性增强（P〈0.01）,MDA含量下降（P〈0.05）,SOD/MDA升高（P〈0.01）。结论茶多酚可提高小鼠血液抗氧化能力,以血清SOD活性增强为主要表现。     

葡萄籽提取物对人体抗氧化能力的影响

目的：研究葡萄籽提取物对人体抗氧化能力的影响。方法：选年龄在45～65岁的健康志愿者100人,根据血清超氧化物歧化酶（superoxide dismutase,SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH Px）活性和丙二醛（maleic dialdehyde,MDA）含量,随机分为试食组和对照组,每组50人,试食组受试者每天口服葡萄籽提取物软胶囊1g,连续用3个月,对照组不服药。试验前后每人测一次血清中SOD、GSH-Px、MDA三项指标。结果：试验后试食组血清SOD、GSH-PX显著高于对照组（P均＜0.05）,试食组血清MDA含量显著低于对照组（P＜0.01）;且试食组试验前后自身比较三项指标亦有统计学差异（P均＜0.01）。结论：葡萄籽提取物具有明显的体内抗氧化活性。     

葡萄籽原花青素改善睡眠功能试验研究

目的 观察葡萄籽原花青素（grape seed proanthocyanidin,GSP）对小鼠睡眠的影响。 方法 将小鼠随机分为对照组和高、中、低3个剂量组,通过改善睡眠功能试验,观察GSP对小鼠睡眠及血清谷胱甘肽（GSH）含量、大脑谷胱甘肽过氧化物酶（GSH-Px）活性和血清、大脑丙二醛（MDA）含量的影响。 结果 GSP能提高小鼠睡眠率、延长小鼠睡眠维持时间、增加血清GSH含量和大脑GSH-Px活性、降低血清和大脑MDA含量。 结论 GSP具有改善小鼠睡眠和增强大脑抗氧化能力的作用。     

原花青素对小鼠耐力和抗氧化酶活性的实验研究

目的研究原花青素对小鼠耐力和血中丙二醛（MDA）、超氧化物歧化酶（SOD）水平的影响,进而探讨原花青素的抗衰老作用机制。方法取小鼠随机分为原花青素大、小剂量组和对照组,然后行小鼠游泳耐疲劳、常压耐缺氧实验,测定并比较原花青素组与对照组血清MDA含量和SOD的活性。结果原花青素可以延长小鼠力竭游泳时间,提高抗疲劳能力;延长小鼠常压耐缺氧时间,提高抗耐缺氧能力;同时使血清SOD活力明显升高、MDA含量明显降低,与对照组相比,差异有统计学意义（P〈0.01）。结论原花青素具有良好的体内抗氧化作用,可以延缓衰老。     

新生牛肝提取物生物学活性分析

复肝肽（FGP）系采用膜法提取技术,从新生牛肝脏中提取制备出的生物活性物质。测定其多肽的含量为950．03mg／100ml。本实验对其促生长、免疫增强作用、对化学性肝损伤辅助保护作用等生物学活性进行了研究。结果表明：小鼠体重较对照组有增加趋势（P〉0．05）;腹腔注射2．5、5．0、10．0ml／（kg·bw）复肝肽组小鼠玫瑰花环成环率分别高于对照组（生理盐水）33．3％,34．2％,39．6％,差异显著（P〈0．05）。注射10．0ml／（kg·bw）复肝肽的小鼠血清溶血素OD值与对照组差异显著（P〈0．01）。剂量组小鼠腹腔巨噬细胞吞噬百分率分别比对照组高22．1％,25．8％,26．5％,差异均有显著性（P〈0．05或P〈0．01）,说明复肝肽具有免疫增强作用,且呈剂量依赖性。复肝肽5．Oral／（kg·bw）剂量组可有效降低四氯化碳模型小鼠血清中丙氨酸氨基转移酶（ALT）,差异具有显著性（方差分析,P〈0．01）,病理组织学指标亦有明显改善。说明新生牛肝提取物对化学性肝损伤的辅助保护作用。     

沙棘果提取物对Wistar老龄鼠抗衰老作用的实验研究

目的：观察沙棘果提取物对Wistar老龄鼠抗衰老作用。方法：给Wistar老龄大鼠灌胃沙棘果提取物，25d后，用生化法测定其血清超氧化物歧化酶（SOD）、谷胱甘肽过氧化酶（GSH-Px）及脂质过氧化物（LPO）的降解产物（MDA）的水平，用荧光法测定其大脑皮质脂褐素（Lipofusin）含量。结果：实验组灌胃沙棘果提取物与对照组比较，老龄大鼠体内MDA、Lipofusin含量明显下降；SOD、GSH-Px活力明显提高。结论：沙棘果提取物有降脂质过氧化的作用，有较强的抗氧化功能。     

玛咖提取物对体育运动的抗疲劳作用

目的 探讨玛咖提取物对体育运动的抗疲劳作用。方法 50只雄性Wistar大鼠随机分为,①对照组：正常饲养,不游泳,灌胃等量蒸馏水;②单纯游泳组：游泳,灌胃等量蒸馏水;③游泳+给药组：游泳,并分别灌胃玛咖提取物4.0、8.0和16.0 g/（kg·bw）。每组10只动物。采用循环水流自由游泳,给予大鼠4.0、8.0和16.0 g/（kg·bw）玛咖提取物,连续给药15 d,第16天采样测定肝过氧化脂质（LPO）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和肝糖元水平。结果 与单纯游泳组动物比较,给予玛咖提取物4.0、8.0和16.0 g/（kg·bw）15 d后,大鼠下沉次数分别减少了27.44%、42.86%、64.11%（P<0.01）,下沉前游泳时间和游泳总时间分别延长了18.99%、64.46%、90.69%和37.12%、56.23%、94.72%（P<0.01）,LPO水平分别降低了31.31%、42.00%、56.95%（P<0.01）,SOD、GSH-Px和肝糖原水平分别升高了25.92%,31.82%、62.09%,12.33%,23.01%、46.36%和17.83%、44.69%、62.99%（P<0.01）。结论 玛咖提取物具有降低肝LPO水平,增加肝糖原水平,提高SOD、GSH-Px活性,增强运动耐力和抗疲劳作用。     

救必应对小鼠急性化学性肝损伤的保护作用

目的研究救必应的水提物（IRTW）对小鼠急性化学性肝损伤的保护作用及其机制。方法将60只小鼠随机平均分成6组：正常对照组、CCl4模型组、BPD组及IRTW高、中、低三个剂量组;采用四氯化碳（CCl4）诱导小鼠急性肝损伤模型,观察IRTW对小鼠血清丙氨酸氨基转移酶（ALT）、门冬氨酸氨基转移酶（AST）活性和肝匀浆超氧化物歧化酶（SOD）、丙二醛（MDA）、谷光甘肽过氧化物酶（GSH-Px）含量及对肝组织病理变化的影响。结果 IRTW各剂量组能显著降低小鼠血清ALT、AST含量（P〈0.01或P〈0.05）,降低肝匀浆MDA含量（P〈0.01或P〈0.05）,升高SOD、GSH-Px活性（P〈0.01或P〈0.05）,减轻肝组织病理损伤程度。结论 IRTW对小鼠急性化学性肝损伤具有显著保护作用,其作用机制可能与抗脂质过氧化有关。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.