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Oral lichen planus (OLP) is considered a T cell-mediated autoimmune disease with unknown aetiology. T helper cells appear to play an important role in the pathogenesis of OLP. We investigated the possible role of T helper cells, Th1 and Th17, in the lesions and circulation of patients with OLP. Forty patients with OLP and 15 healthy volunteers were recruited. Double immunofluorescence staining was used to detect Th1 and Th17 cells in the OLP lesions, and intracellular cytokine staining and flow cytometry to evaluate the proportion of Th1 and Th17 cells in peripheral blood. The levels of serum interferon (IFN)-γ and interleukin (IL)-17 were assessed by using an enzyme-linked immunosorbent assay. It was found that Th17 cells, as well as Th1 cells, were present in OLP lesions. The proportion of peripheral Th1 and Th17 cells was significantly increased in patients with OLP. The proportion of Th17 cells in atrophic-erosive OLP was elevated as compared with that in reticular OLP. Serum IL-17 levels in OLP patients were significantly higher than in controls, and those in the atrophic-erosive OLP group were increased as compared with the reticular OLP group. However, the levels of serum IFN-γ were slightly decreased in OLP patients. Our data suggested that Th1 and Th17 cells in the local lesions and peripheral blood may be associated with the pathogenesis of OLP, and that IL-17 may be an important proinflammatory cytokine in OLP. These findings enhance our understanding of OLP pathogenesis.  相似文献   

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To investigate the expression of vaginal Th1 and Th2 cytokines in rats with experimental vaginal candidiasis under different immune conditions, ICR murine vaginal candidiasis model was established and immno-suppressed murine models of vaginal cadidiasis were established in estrogen-treated mice. Non-estrogen-treated mice were used as controls. The mRNA level of Th1 (IL-2)/Th2 (IL-4, IL-10, TGF-β1) cytokines in murine vaginal tissues was determined by RT-PCR. The cykotine in local tissues was increased to different extent under normal immune condition. IL-2 mRNA was increased during early stage of infection, while IL-10 was increased transiently during late stage of infection. TGF-β1 production was found to be increased persistently. At same time, the expression of IL-2 mRNA was suppressed in immno-suppressed group, and the level of IL-4, IL-10, and TGF-β1 were higher than the normal immunity group to different degree during infection. The high level of IL-2 mRNA during early stage of infection was associated with clearance of mucosal Candidia albicans (C. albicans), and its expression suppressed leading to decreased clearance of mucosal C. albican in immuno-suppression. The over-expression of IL-4 and IL-10 could significantly enhance the susceptibility to C. albicans infection in mice.  相似文献   

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Expression of stromal cell-derived factor-1 in diabetic retinopathy   总被引:1,自引:0,他引:1  
Background Neovascularization can cause vision loss in proliferative diabetic retinopathy (PDR) and may be affected by many factors. Stromal cell-derived factor-1 (SDF-1) is a potent stimulator of angiogenesis. The study was aimed to investigate the expression of SDF-1 and its correlation with vascular endothelial growth factor (VEGF) in the eyes with diabetic retinopathy. Methods The levels of SDF-1 and VEGF were measured by enzyme-linked immunosorbent assay in the vitreous of 41 eyes of 41 patients with PDR and 12 eyes of 12 patients with idiopathic macular hole (IMH). Vitreous fluid samples and fibrovascular preretinal membranes were obtained at vitrectomy. SDF-1 and VEGF were localized using immunohistochemistry. Results The vitreous concentration of VEGF was significantly higher in eyes with PDR ((2143.7~1685.21) pg/ml) than in eyes with IMH ((142.42~72.83) pg/ml, P 〈0.001). The vitreous level of SDF-1 was also significantly higher in eyes with PDR ((306.37~134.25) pg/ml) than in eyes with IMH ((86.91~55.05) pg/ml, P 〈0.001). The concentrations of both VEGF and SDF-1 were higher in eyes with active PDR than in eyes with inactive PDR. Panretinal photocoagulation (PRP) could decrease the SDF-1 levels in the vitreous of PDR patients. The vitreous concentration of SDF-1 correlated with that of VEGF in eyes with PDR (r=0.61, P 〈0.001). The costaining of SDF-1 and VEGF was confined to the vascular components in preretinal membranes. Conclusions SDF-1 protein is highly expressed in both the vitreous and preretinal membranes of PDR patients; SDF-1 may be correlated with VEGF in angiogenesis in PDR.  相似文献   

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The role of Fas/Fas ligand (FasL) in mediating alveolar macrophage (AM)-derived inflammatory cytokines in 29 human lung silicosis patients and 6 controls was studied. It showed that the serum levels of IL-1β, TNF-a, IL-8, MIP-la, MCP-1, and TGF-β1 were higher in silicosis patients than in controls, and increased with the progression of silicosis except TGF-β1 and IL-8. TGF-β1, IL-8, MCP-1,  相似文献   

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The cytokine repertoire of ADP/ATP carrier-specific humoral immune responses and the cytokine-dependent anti-ADP/ATP carrier antibody IgG subclasses were examined in a cohort of ADP/ATP carrier-immunized BALB/c mice treated with anti-CD4 monoclonal antibody. Eighteen male BALB/c mice (6–8 weeks old) were randomized into 3 groups: dilated cardiomyopathy (DCM) group, DCM-tolerance (Tol) group and control group. The mice in DCM group were immunized with the peptides derived from human ADP/ATP carrier protein for 6 months and mice in the control group were sham-immunized, while the mice in DCM-Tol group were immunized with ADP/ATP carrier protein and anti-CD4 McAb simultaneously. Serum autoantibody against ADP/ATP carrier and IgG subclasses were measured by ELISA, intracellular cytokines IFN-γ and IL-4 of Th cells were moni- tored with flow cytometry, and splenic T cell cytokines IFN-γ, IL-2, IL-4 and IL-6 were detected by using real-time fluorescent quantitative PCR. The results showed that the autoantibody against ADP/ATP carrier was found in all mice in DCM group, and the antibody level, serum IgG1 and IgG2a subclasses, cytokines in T cells and Th cells were all elevated in DCM group, as compared with those in control group (P〈0.01). On the other hand, in DCM-Tol group, the autoantibody level and contents of all the cytokines were significantly different from those in DCM group (P〈0.01), and were close to those in control group. And the levels of IgG1, IgG2a, IgG2b and IgG3 were influenced, to varying degrees, by anti-CD4 McAb as compared with those in DCM group. All these four types of IgG subclasses were substantially decreased in DCM-Tol group as compared with DCM group. It is concluded that the treatment with anti-CD4 McAb could prevent the activation of T cells, reverse the abnormal secretion of cytokines and the imbalance between Th1/Th2 cell subsets and abnormal production of autoantibody against ADP/ATP carrier, and eventually avoid myocardial injuries.  相似文献   

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This study examined the role of regulated upon activation normal T cell expressed and secreted(RANTES) and its receptor C-C chemokine receptor type 5(CCR5) in gastric cancer metastasis and the associated mechanism.The expression of RANTES and CCR5 was detected by using immunohistochemical staining and Western blotting in the gastric cancer tissues obtained from 60 gastric cancer patients with or without lymph node metastasis(n=30 in each).The results showed that the expression levels of RANTES and CCR5 were higher in gastric cancer with lymph node metastasis than in that without metastasis(P<0.05).The expression levels of RANTES in 30 lymph nodes with cancerous invasion were higher than in 30 normal lymph nodes(P<0.05).Chemotactic test revealed that the number of migrating gastric cancer cells(n=295.0±54.6) induced by the protein of cancer-invading lymph nodes was greater than that by the protein mixture from cancer-invading lymph nodes and RANTES antibody(n=42.5±11.6)(P<0.05).RT-PCR showed that the expression levels of the main Th1 cytokines(IL-2,γ-IFN) were lower in gastric cancer with lymph node metastasis(2.22±0.90,3.26±1.15 respectively) than in that without metastasis(3.07±1.67,4.77±1.52 respectively)(P<0.05),but the expression level of the main Th 2 cytokine(IL-10) was higher in gastric cancer with lymph nodes metastasis(6.06±2.04) than in that without metastasis(4.88±1.87)(P<0.05).It was concluded that RANTES and its receptor CCR5 may contribute to gastric cancer metastasis through influencing the balance of Th1/Th2.RANTES and CCR5 may become a marker of gastric cancer metastasis.  相似文献   

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Background Otitis media with effusion (OME) is a disease with complicated pathogeneses which are not clearly known Increasing interest has been focused on immunological cells, cytokines and their roles in chronic inflammatory states. This study was designed to disclose the existence and roles of interleukin-10 (IL-10) and transforming growth factor beta1 (TGF-β1) in the cause of OME in adults, and to investigate the probable role of Foxp3^+CD4^+CD25^+ T cells in OME. Methods The concentrations of IL-10 and TGF-β1 in the middle ear effusions (MEEs) and plasmas of 36 adults (45 ears) with OME were measured by means of enzyme linked immunosorbent assay (ELISA). As contrast, the concentrations of IL-10 and TGF-131 in the plasma of 30 normal volunteers were measured using the same method. Furthermore, the proportion of Foxp3^+CD4^+CD25^+ T cells in CD4^+ T cells of blood was tested by flow cytometry. Results (1) The concentrations of IL-10 in all MEEs and plasmas of the chronic OME patients were higher than those in patients with acute OME (both P 〈0.05), so was TGF-131 (both P 〈0.01). The concentration of IL-10 in MEEs was significantly higher than that in plasmas, not only in acute OME (P〈0.01), but also in chronic OME (P〈0.01). In chronic OME, the concentration of TGF-β1 in MEEs had no statistical difference with those in plasmas of the same patients. However, the concentration of TGF-β1 in plasmas of patients with chronic OME was significantly higher than that in plasmas of normal volunteers (P 〈0.01). (2) The concentrations of IL-10 and TGF-β1 in MEEs of the patients who had been treated more than once were higher than those MEEs of the patients who were treated for the first time, respectively (P〈0.05, P〈0.01). The level of TGF-β1 in plasmas of the patients who had been treated more than once was higher than in those of the patients who were treated firstly (P 〈0.05), while the level of IL-10 in plasmas had no difference. The concentration of IL-10 in mucoid MEEs was higher than those in serous ones (P〈0.05), while TGF-β1 had no statistical difference between mucoid and serous MEEs (P〉0.05). The concentration of IL-10 in MEEs had a strong correlation with the duration of the illness (r=0.547, P〈0.01). The same correlation was also found between the concentration of TGF-β1 in MEEs and the times patients being treated (r=0.579, P 〈0.01). (3) The proportion of Foxp3^+CD4^+CD25^+T/CD4^+ T cells in the blood of chronic OME was not only significantly higher than that in the acute OME (P〈0.01), but also higher than that in normal volunteers (P 〈0.01). In chronic OME, there was a correlation between the proportion of Foxp3^+CD4^+CD25^+ T/CD4^+ T cells in the blood and the concentration of IL-10 in the plasmas (r=0.602, P 〈0.05). Conclusions IL-10 and TGF-β1, as two important immunoregulatory mediators, participate in middle ear inflammatory response, especially in chronic course of OME in adults. Foxp3^+CD4^+CD25^+ T cells may play some immunoregulatory roles in the course of this disease.  相似文献   

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Background Airway remodeling is the specific pathological characteristics of asthma, which is related to the clinical symptoms, pulmonary function, and airway hyperreactivity. This study aimed at exploring the effects of dermatophagoides pteronyssinus allergen-specific immunotherapy (SIT) on the serum interleukin (IL)-13 and pulmonary functions in asthmatic children.
Methods Fifty-eight pediatric asthma patients allergic to dust mite participated in this study. Thirty-five children received SIT with a standardized dermatophgoides pteronyssinus extract for one year (SIT group), and the other 23 children treated with inhaled corticosteroids (ICS group) according to the Global Initiative for Asthma (GINA) for one year. Serum levels of IL-13, IL-4 and interferon (IFN)-γ were examined and the pulmonary functions were checked before and after the treatment.
Results After the treatment, the number of emergency visiting for asthma attack in SIT group was significantly less than that in ICS group. The serum levels of IL-4 and IL-13 were clearly reduced, IFN-γ and the ratio of IFN-γ/IL-4 were significantly increased, the pulmonary functions (forced vital capacity (FVC), forced expiratory volume in one second percentage (FEV1%) and peak expiratory flow percentage (PEF%) were significantly improved in the SIT group. Meanwhile, IFN-γ and the ratio of IFN-γ/IL-4 were greatly increased, but serum levels of IL-4 and IL-13 had less changes, the pulmonary functions (FVC, FEV1% and PEF%) were poorly improved in ICS group. The basic pulmonary functions in both groups were at the same level, which had made more improvement in SIT group than in ICS group one year later.
Conclusions One year of dermatophagoides pteronyssinus SIT can significantly reduce the frequencies of emergency visiting for asthma attack and improve the pulmonary functions of children with allergic asthma, and that is attributed to SIT, which can reduce the levels of IL-4 and IL-13 and regulate the imbalance of the Th1/Th2 cells in asthmatic children. All of these might be effective in preventing the asthmatic airway from remodeling.  相似文献   

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目的比较中国儿童及成人艾滋病(AIDS)患者的免疫活化水平。方法以103例儿童AIDS患者、38例成人AIDS患者、88名健康儿童和72名健康成人为研究对象,取抗凝血进行流式细胞计数检测CD4 T细胞数,荧光定量RT-PCR测定血清HIV-RNA滴度,采用ELISA法检测血清中Th1/Th2免疫因子(IL-10、IL-16、IL-18)、趋化因子[包括调节活化正常T细胞表达与分泌的趋化因子(RANTES)、单核细胞趋化蛋白1(MCP-1)、基质细胞衍生因子1(SDF-1)]及巨噬细胞刺激蛋白(MSP)的水平,并以β2微球蛋白(β2-MG)和可溶性Fas(sFas)的表达水平作为机体免疫活化指标。结果儿童AIDS患者组的CD4 T细胞数显著低于儿童正常对照组(P<0.01),各细胞因子水平较儿童正常对照组显著升高(P均<0.01)。成人AIDS患者组的CD4 T细胞数显著低于成人正常对照组(P<0.01),除MSP水平显著低于成人对照组(P<0.01)外,其余各细胞因子均显著高于成人对照组(P<0.01)。儿童AIDS患者组各因子水平除SDF-1α和β2-MG与成人AIDS患者组差异无显著性(P>0.05)外,其余均显著高于成人AIDS患者组(P<0.01)。结论儿童和成人AIDS患者的免疫系统均表现为明显的异常活化,儿童患者的免疫活化水平高于成人患者。  相似文献   

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目的:检测过敏性疾病患者血清Th1细胞因子IFN-γ和Th2细胞因子IL-4、IL-5、IL-13以及趋化因子Eotaxin、RANTES、LTB4的水平,探讨其临床意义.方法:过敏性疾病患者64例,正常对照21例.采用双抗体夹心酶联免疫吸附实验(ELISA)检测血清IL-4、IL-5、IL-13、IFN-γ、LTB4、RANTES和 Eotaxin水平.结果:①过敏性疾病患者血清IL-4、IL-5、IL-13和 Eotaxin、LTB4水平较对照组明显增高,差异有统计学意义(P<0.05).②IFN-γ和RANTES在过敏性疾病患者血清中的水平低于对照组,但无统计学差异(P>0.05).③IL-4、IL-5、IL-13和LTB4彼此之间呈显著正相关(P<0.01).④Eotaxin、RANTES和IFN-γ彼此之间呈显著正相关(P<0.05).⑤LTB4和Eotaxin之间呈显著正相关(P<0.01).结论:过敏性疾病的发生存在多种细胞因子和趋化因子复杂的相互作用.  相似文献   

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王丹  陈秋霞  孟琼  陈铭珍  曹军 《右江医学》2004,32(3):201-202
目的 探讨IL -18、sFas和sFasL在特应性皮炎 (AD)发生发展中的作用。方法 采用固相酶联免疫分析 (ELISA)法检测 62例AD患儿和 2 5例正常儿童血清中IL -18、sFas和sFasL的含量。结果 AD患儿血清中的IL -18、sFas和sFasL水平与正常对照组比较有明显增高 (P <0 .0 1) ,且AD患儿血清中的IL -18增高与sFas水平增高呈正相关 (r =0 .472 ,P <0 .0 1)。结论 IL -18、sFas与sFasL均参与AD免疫发病过程 ,IL -18、sFas的血清水平检测有助于了解疾病的活动性、判断病情及监测治疗效果。  相似文献   

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目的 分析杂合式血液净化对维持性血液透析患者β2微球蛋白(β2-MG)水平及微炎症状态的影响.方法 选取2015年4-10月重庆医科大学附属第一医院及重庆医科大学附属第一医院第一分院收治的维持性血液透析患者128例,将其分为单纯血液透析组与杂合式血液净化组,各64例.于治疗前及治疗6个月后,分别采集患者透析前血标本,测定β2-MG、超敏C反应蛋白(hs-CRP)与白细胞介素-6(IL-6)水平,并进行比较分析.结果 治疗6个月后,杂合式血液净化组患者血β2-MG、hs-CRP、IL-6水平较治疗前明显降低,差异均有统计学意义(P<0.05);而单纯血液透析组患者血β2-MG水平较治疗前降低,hs-CRP、IL-6水平较治疗前升高,但差异均无统计学意义(P>0.05);治疗6个月后,杂合式血液净化组患者血β2-MG、hs-CRP、IL-6水平均低于单纯血液透析组,差异均有统计学意义(P<0.05).结论 杂合式血液净化可以更有效地清除透析患者血液中分子毒素,并减轻机体慢性微炎症状态.  相似文献   

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OSAHS患儿血清Th1/Th2细胞因子检测及临床意义   总被引:1,自引:0,他引:1       下载免费PDF全文
目的 探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患儿血清Th1/Th2免疫平衡相关细胞因子的检测及临床意义.方法 收集61例OSAHS患儿内镜下腺样体阻塞程度数据,并测定患儿血清中Th1细胞因子-肿瘤坏死因子β(TNF-β)和干扰素-γ(IFN-γ),Th2细胞因子IL-10、IL-13和IL-12的水平.同时收集26名正常儿童样本为对照组,进行相同血清细胞因子检测.结果 OSAHS患儿血清IFN-γ的水平要明显低于正常对照组儿童,差异有统计学意义(P〈0.01);但OSAHS 患儿其他血清Th1/Th2免疫平衡相关细胞因子(TNF-β、IL-10、IL-13和IL-12)与对照组之间差异无统计学意义(P〉0.05).OSAHS患儿IFN-γ水平与其腺样体肥大阻塞后鼻孔程度之间并无相关性.结论 Th1和Th2免疫反应间存在制衡机制.儿童OSAHS病例中,血清IFN-γ的水平下降表明,由此导致Th1细胞介导的保护性细胞免疫反应下降,Th1/Th2失衡,从而使儿童腺样体扁桃体过度肥大而发病.  相似文献   

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OSAHS患儿血清Th1/Th2细胞因子检测及临床意义   总被引:1,自引:0,他引:1       下载免费PDF全文
目的 探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患儿血清Th1/Th2免疫平衡相关细胞因子的检测及临床意义。方法 收集61例OSAHS患儿内镜下腺样体阻塞程度数据,并测定患儿血清中Th1细胞因子—肿瘤坏死因子β(TNF-β)和干扰素-γ(IFN-γ),Th2细胞因子IL-10、IL-13和IL-12的水平。同时收集26名正常儿童样本为对照组,进行相同血清细胞因子检测。结果 OSAHS患儿血清IFN-γ的水平要明显低于正常对照组儿童,差异有统计学意义(P<0.01);但OSAHS 患儿其他血清Th1/Th2免疫平衡相关细胞因子(TNF-β、IL-10、IL-13和IL-12)与对照组之间差异无统计学意义(P>0.05)。OSAHS患儿IFN-γ水平与其腺样体肥大阻塞后鼻孔程度之间并无相关性。结论 Th1和Th2免疫反应间存在制衡机制。儿童OSAHS病例中,血清IFN-γ的水平下降表明,由此导致Th1细胞介导的保护性细胞免疫反应下降,Th1/Th2失衡,从而使儿童腺样体扁桃体过度肥大而发病。  相似文献   

17.
目的探讨脑囊虫病患者经吡喹酮治疗后,机体免疫应答的状态和对病程转归影响。方法35例脑囊虫病患者经吡喹酮治疗6个月后,对血清中IL-2、INF-γ、IL-5、IL-10、TNF-α、IL-12进行检测。结果治疗6个月后,Th1型细胞因子IL-2、IFN-γ、IL-12明显升高(P<0.01),Th2型细胞因子IL-5和IL-10在患者中检出率明显下降(P<0.01),TNF-α水平较治疗前显著下降(P<0.05)。治疗组对药物反应良好的患者,IL-2、IFN-γ、IL-12明显高于对药物反应不良者。结论脑囊虫病患者治疗后Th2型细胞因子反应减弱,而Th1型细胞因子反应增强,机体的保护性免疫应答已经开始恢复。  相似文献   

18.
初发SLE患者Th1/Th2及其调控因子基因的研究   总被引:2,自引:0,他引:2  
目的:探讨未经药物治疗初发狼疮病人Th1/Th2细胞亚群分布及其调控细胞因子基因表达的差异。方法:运用三色荧光标记流式细胞术检测35例初发狼疮病人细胞亚群分布,并以10例正常人作对照;ABI 7700real-time PCR法同时检测38例病人和28例正常人IL-10、IL-12P40、IL18mPNA表达水平的差异。结果:1、初发狼疮病人Th1较正常人明显减低(P<0.05),但Th1/Th2无显著性改变。2、与正常组相比,SLE组病人IL-12P35、IL-12P40、IL-18mRNA及其受体表达较正常人明显降低(P均<0.05);3、面部红斑组病人Th1/Th2、IL-12P35较正常组降低(P均<0.05);4、RNP阳性组病人IL-12P40较正常组升高,IL-12P35、IL-18较正常组降低(P均<0.05);5、有关节炎较无关节炎患者IL-18RmRNA表达升高(P<0.05)。结论:SLE是一种以Th1细胞下降,Th2细胞相对占优势的自身免疫性疾病,源于诱导向Th1细胞分化的一系列细胞因子及其受体减少和细胞因子间失衡所致。  相似文献   

19.
目的:探讨血、尿β2-微球蛋白(8rMG)对糖尿病患者的临床意义。方法:用放射免疫方法测定糖尿病患者血、尿β2-MG的水平。结果:糖尿病患者血、尿β2-MG含量明显高于健康人员(P〈0.01),且随着病程的延长,血、尿β2-MG的含量明显增加;血、尿β2-MG的含量在非胰岛素依赖性糖尿病(NIDDM)和胰岛素依赖性糖尿病(IDDM)患者之间比较,差异无统计学意义(P〉0.05)。结论:测定糖尿病患者血、尿β2-MG对早期发现、预防和治疗糖尿病肾病有重要的临床意义。  相似文献   

20.
目的 通过对充血性心力衰竭(CHF)患者细胞凋亡调节因子Fas,Fas-L及其他细胞因子的研究,以探讨CHF的病理生理,初步讨论细胞凋亡与CHF的关系。方法 按NYHAFC,选择CHF病例125例及24例年龄,性别匹配正常对照者。运用ELISA法测sFas及sFas-L,放射免疫法测IL-6,NO,ET,并进行相关研究。结果 CHF患者各细胞因子水平明显高于正常对照组,并随CHF程度加重而升高。结论 CHF患者神经内分泌过度激活,心肌细胞存在凋亡,随心衰程度加重,凋亡加剧。各细胞因子直接或间接调节细胞凋亡,在CHF中起重要作用。  相似文献   

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