中药灯盏细辛化学成分的研究(Ⅱ)

目的：进一步研究灯盏细辛「Ｅｒｉｇｅｒｏｎ ｂｒｅｖｉｓｃａｐｕｓ（Ｖａｎｔ．）Ｈａｎｄ．－Ｍａｚｚ．」的化学成分。方法：采用硅胶柱层析对灯盏细辛的氯仿萃取部分的化学成分进行分离和纯化,应用理化常数和光谱分析（ＩＲ,ＭＳ,＾１ＨＮＭＲ,＾１３ＣＮＭＲ）鉴定其结构。结果：得到６个单体化合物,结构分别为３,４－二羟基肉桂酸（３,４－ｄｉｈｙｄｒｏｘｙ－ｐｈｅｎｙｌ ａｃｒｙｌｉｃ ａｃｉｄ,Ⅶ）,α－甲氧基－γ－吡喃酮（α－ｍｅｔｈｏｘｙ－γ－ｐｙｒａｎｏｎｅ,Ⅷ）、豆甾醇（ｓｔｉｇｍａｓｔｅｒｏｌ,Ⅺ）、胡萝卜甙（β－ｓｉｓｔｏｓｔｅｒｏｌ－３－Ｏ－β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｉｄｅ,Ⅻ）。结论：化合物Ⅶ,Ⅷ,Ⅺ,Ⅻ为首次从本植物中分得。     

光皮木瓜化学成分的研究(Ⅱ)

目的：研究光皮木瓜［Ｃｈａｅｎｏｍ ｅｌｅｓｓｉｎｅｎｓｉｓ（Ｔｈｏｕｉｎ．） Ｋｏｅｈｎｅ］果实中的化学成分,为进一步开发利用木瓜资源提供理论依据。方法：采用溶剂法和层析法分离、纯化光皮木瓜的化学成分,并通过理化性质与波谱数据,鉴定所得化合物的分子结构。结果和结论：从光皮木瓜果实的乙醇提取物中分离得到７个单体化合物,分别鉴定为棕榈酸（Ⅴ）、乌索酸－３－Ｏ－山嵛酸酯（Ⅵ）、乌索酸（Ⅶ）、３－乙酰乌索酸（Ⅷ）、３－乙酰坡模醇酸（Ⅸ）、胡萝卜苷（Ⅹ）和桦木酸（Ⅺ）。所有化合物均是首次从该植物中分离得到,其中化合物Ⅵ为一新化合物。     

粗叶悬钩子化学成分的分离鉴定

目的：研究粗叶悬钩子化学成分。方法;用色谱法和光谱解析方法对粗叶悬钩子叶的化学成分进行分离和结构测定。结果：共得到６个化合物,粗叶悬钩子甙（ａｌｃｅｓｅｆｏｌｉｓｉｄｅ,１）,金丝桃甙（ｈｙｐｅｒｏｓｉｄｅ,２）,３－氧化－６－羟基－柴罗兰醇（ｖｏｍｉｆｏｌｉｏｌ,３）,β－谷甾醇（β－ｓｉｔｏｓｔｅｒｏｌ,４）,胡锣卜甙（ｄａｕｃｏｓｔｅｒｏｌ,５）,碳三十二醇（ｄｏｔｒｉａｃｏｎｔｙｌ ａｌｃ     

中药羊蹄化学成分的研究(Ⅰ)

目的：研究蓼科药用植物羊蹄（Ｒｕｍｅｘ ｊａｐｏｎｉｃｕｓ Ｈｏｕｔｔ．）根的化学成分。方法：采用溶剂法和层析法分离、纯化羊蹄的化学成分,并通过理化性质与光谱数据,鉴定所得化合物的分子结构。结果：从羊蹄根的乙醇提取物中分离得到８个单体化合物,分别鉴定为大黄酚（Ⅰ）、大黄素甲醚（Ⅱ）、大黄素（Ⅲ）、酸模索（Ⅳ）、β－谷甾醇（Ⅴ）、大黄酚８－Ｏ－β－Ｄ－吡喃葡萄糖苷（Ⅵ）、大黄素甲醚８－Ｏ－β－Ｄ－吡喃葡萄糖苷（Ⅶ）及大黄素８－Ｏ－β－Ｄ－吡喃葡萄糖苷（Ⅷ）。其中化合物Ⅵ,Ⅶ,Ⅷ为首次从该植物中分离得到。结论：分离到的８个单体化合物为羊蹄活性成分的深入研究和药用资源的开发利用提供了实验依据。     

假鹰爪化学成分研究(Ⅱ)

目的：寻找番荔枝科假鹰爪属植物假鹰爪Ｄｅｓｍｏｓ ｃｈｉｎｅｎｓｉｓ抗肿瘤活性成分。方法：采用硅胶柱色谱分离化学成分。根据理化性质及波谱分析等方法鉴定结构。结果：从氯仿萃取物中分离鉴定了７个化合物,分别是ｕｎｏｎ－ａｌ（Ⅰ）,黄芩素－７－甲醚（Ⅱ）,去甲氧杜鹃花素（Ⅲ）,去甲氧杜鹃花素－７－甲醚（Ⅳ）,苯甲酸（Ⅴ）,β－谷甾醇（Ⅵ）和豆甾醇（Ⅶ）。结论：该种植中鉴定的黄酮类化合物均为β－环未取代,     

景洪哥纳香中四个新生物碱的结构鉴定

目的：对云南西双版纳产的景洪哥纳香（Ｇｏｎｉｏｔｈａｌａｍｕｓ ｃｈｅｌｉｅｎｓｉｓ Ｈｕ．）树皮的化学成分进行分离和结构鉴定。方法：采用各种色谱技术进行分离,根据理化性质和光谱数据进行结构鉴定。结果：分离得到４个化合物,鉴定为景洪哥纳香碱（ｃｈｅｌｉｅｎｓｉｓｉｎｅ,Ⅰ）,景洪哥纳香胺（ｃｈｅｌｉｅｎｓｉｓａｍｉｎｅ,Ⅱ）,景洪哥纳香内酰胺甲（１０－氨基－４－羟基－３,８－二甲氧基菲－１－羧酸内酰胺,ｃｈｅｌｉｅｎｓｉｓａｍ Ａ,Ⅲ）,景洪哥纳香同酰胺乙（１０－氨基－４,８－二羟基－３－甲氧基菲－１－羧酸内酰胺,ｃｈｅｌｉｅｎｓｉｓａｍ Ｂ,Ⅳ）。结论：所有化合物均为新化合物,其中Ⅰ和Ⅱ为从哥纳香属中发现的两种新结构类型的生物碱,Ⅲ和Ⅳ为首次发现的Ａ环为４－羟基－３－甲氧基取代的菲内酰胺生物碱。     

中药灯盏细辛化学成分的研究(Ⅰ)

研究灯盏细辛「Ｅｒｉｇｅｒｏｎ ｂｒｅｖｉｓｃａｐｕｓ（Ｖａｔ．）Ｈａｎｄ．－Ｍａｚｚ．」的化学成分。方法采用柱层析对灯盏细的苯溶解部位中的化学成分进行分离和纯化,应用理化党数和光谱分析（ＩＲ,ＭＳ,＾１Ｈ－ＮＭＲ,＾１３）鉴定其结构。结果：得到６个单体化合物,结构分别为木栓酮（ｆｒｉｅｄｅｌｉｎ,Ⅰ）木栓烷（ｆｒｉｅｄｅｌａｎｅ,Ⅱ）,木栓醇（ｆｒｉｅｄｅｌｉｎｏｌ,Ⅲ）,表木栓醇（ｅｐｉｆｒｉ     

密蒙花三萜等成分的研究

目的：寻找中药密蒙花（Ｂｕｄｄｌｅｉａｏｆｆｉｃｉｎａｌｉｓ）的抗菌消炎成分。方法：用色谱法对花蕾的亲脂性化学成分进行分离。结果：共分离得到６个化合物：齐墩果－１３（１８）－烯－３－酮（１），δ－香树脂醇（２），大戟烷－８，２４－二烯－３－醇乙酸酯（３），α－菠甾醇（４），半乳糖醇（５）和香豆酸（６）。结论：所有化合物均为首次从该植物分得，化合物１是首次从天然界中获得。     

女贞子化学成分的研究

女贞子化学成分的研究硕士研究生尹双指导教师吴立军，王素贤沈阳药科大学药物研究所１１００１５我们从女贞属植物女贞子（ＬｉｇｕｓｔｒｕｍｌｕｃｉｄｕｍＡｉｔ．）中分离得到了１７个化合物，其中的１２个化合物经理化性质及波谱分析分别鉴定为：β－谷甾醇（β－ｓ...     

大叶马蹄香根中的黄酮类成分

目的：研究大叶马蹄香（Ａｓａｕｒｍ ｍａｘｉｍｕｍ Ｈｅｍｓｌ。）根中的化学成分。方法：用溶剂提取、色谱法分离大叶马蹄香的化学成分,并用理化常数和波谱法测定其结构。结果：从大叶马蹄香根的乙醇提取于物中分离到５个黄酮类成分,根据理化性质和汉谱数据鉴定其结构分别为轴皮素（１）,轴皮素２－Ｏ－β－Ｄ吡喃区区糖苷（Ⅱ）,柚皮轴０５,７－Ｏ－β－Ｄ－２吡喃葡萄糖苷（Ⅲ）,４,２＇,４＇,６＇－四羟基查耳酮－     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…