丹皮酚对豚鼠心肌细胞动作电位及钙通道电流的影响

目的 :研究丹皮酚对分离的单个豚鼠心肌细胞动作电位及钙通道电流 (ICa)的影响。方法 :单个细胞膜片钳技术。结果 :①丹皮酚 40 0 μg/ml可使动作电位时程 (APD)明显缩短。APD50 和APD90 分别由给药前的 (35 2±2 7)ms和 (416± 33)ms缩短至 (16 8± 2 0 )ms和 (2 6 5± 2 3)ms(P <0 .0 5 ,n =5 ) ,分别缩短了 5 2 .2 %和 35 .6 % ,而静息电位和动作电位幅值无明显改变 ;② 5 0～ 40 0 μg/ml丹皮酚浓度依赖性阻滞ICa,使其最大峰值由 (916 .7± 197.3)pA分别降至 (5 83 .3± 10 8.8)pA和 (2 5 0 .0± 12 0 .0 )pA(P <0 .0 1) ,抑制率分别为 36 .4%和 72 .7% ,并使ICa的I V曲线上移 ,但不使I V曲线发生偏移。结论 :丹皮酚对ICa的阻滞作用为其抗心律失常作用的主要机制之一     

三七总皂甙对大鼠心肌细胞L-型钙电流的抑制作用

目的 :探讨三七总皂甙对大鼠心肌细胞L -型钙电流 (ICa-L)的抑制作用 .方法 :采用全细胞膜片钳方法分别观测不同浓度三七总皂甙对大鼠心肌细胞L -型钙电流的抑制作用 .结果 :PNS 10 0mg/L和30 0mg/L对ICa -L的抑制率分别为 (2 6 73± 4 5 ) %和 (4 9± 19 6 ) % (P <0 0 1) .结论 :三七总皂甙能阻滞大鼠心肌细胞ICa -L,并呈剂量依赖性     

冬虫夏草对豚鼠心室肌细胞胞浆钙离子浓度及L-型钙电流的影响

目的　研究冬虫夏草 (Codycepssinensis,CS)水提液对豚鼠单个心室肌细胞胞浆内钙离子浓度 ([Ca2 ]i)及L 型钙电流 (ICa L)的影响。方法　酶解法分离豚鼠单个心室肌细胞 ,应用激光扫描共聚焦显微镜联合全细胞膜片钳技术测定豚鼠心室肌细胞 [Ca2 ]i以及ICa L的变化。结果　应用 0 1mg/mL (生药浓度 )冬虫夏草水提液 ,在静息状态下对 [Ca2 ]i 无影响 ;对 6 0mmol/LKCl诱导的胞浆 [Ca2 ]i升高有促进作用 ,峰值荧光强度在 12 0s时由12 0 4 3± 2 38 4增加至 185 5 2± 32 1 0 (n =6 ,P <0 0 5 )。膜片钳研究结果表明 ,冬虫夏草水提液可明显促进ICa L,使ICa L从 (- 15 1± 2 3)pA/ pF增加到 (- 19 7± 3 2 ) pA/pF (刺激电压为 10mV ,n =8,P <0 0 1)。 结论　冬虫夏草水提液促进心肌细胞钙内流 ,是该药治疗缓慢型心律失常的机制之一。     

自发性高血压大鼠左心室肌细胞电生理研究

目的　研究自发性高血压大鼠 (SHR)左心室肌细胞的电生理特性。方法　以正常血压Wistar大鼠左心室肌细胞作为对照 ,采用玻璃微电极技术记录动作电位 ,应用膜片钳全细胞技术记录膜离子流 ,观察SHR左心室肌细胞动作电位及膜离子流的改变。结果　①SHR和Wisar大鼠的心脏 /体重比分别为 5 .6 6± 0 .46mg/g(n =2 0 )和 3.7± 0 .2 9mg/g(n =2 8) (P <0 .0 0 1) ,平均细胞膜电容分别为 2 80 .6 8± 6 7.98pF(n =91)和 189.94± 5 6 .5 9pF(n =137) (P <0 .0 5 )。②SHR左心室肌细胞动作电位时程较Wistar大鼠明显延长 [APD50 :2 1.33± 1.5 6ms(n =6 ) ,vs 14.91± 2 .95ms(n =11) ,P <0 .0 0 1;APD90 :16 4.6 7± 4ms ,vs 93.2 7± 10 .5 9ms ,P <0 .0 0 1]。③内向电流 :SHR左心室肌细胞的ICa -L密度与Wistar大鼠间无差异 [6 .93± 1.71pA/pF(n =2 0 ) ,vs 6 .19± 2 .85pA/pF(n =37) ],但前者的慢失活时间常数显著延长 (5 6 .0 1± 13.36ms,vs 43.6 3± 17.89ms,P <0 .0 0 1)。SHR左心室肌细胞的INa密度与Wistar大鼠间无差异 [2 4.6 1± 6 .72 pA/pF(n =16 ) ,vs 2 4.95± 6 .99pA/pF(n =18) ]。④外向电流 :SHR左心室肌细胞IK1内向电流密度显著小于Wistar大鼠 [- 12 0mV时 ,11.3± 2 .2 6 pA/pF(n =17) ,v     

异丙肾上腺素对犬右心室肌细胞L型钙电流不均一性的影响

目的测定犬右心室三层心肌细胞上参与离子流平衡的内向电流L型钙电流(ICa.L)的特性,并研究其对异丙肾上腺素的反应。方法经酶解分离获得犬右心室外膜下细胞、M细胞和内膜下细胞,应用全细胞膜片钳技术,记录并比较不同部位心肌细胞的ICa.L,分析使用异丙肾上腺素前后电流-电压曲线的差异。结果ICa.L的峰值电流密度(pA/pF)在右心室外膜下细胞、M细胞和内膜下细胞分别为-4.868±1.362(n=20),-3.594±0.544(n=20)和-2.874±0.547(n=20),差异有统计学意义(F=33.493,P<0.05)。使用0.5μmol/L异丙肾上腺素后,右心室外膜下心肌细胞ICa.L的峰值电流密度由-4.832±1.127(n=11)增至-22.348±4.057(P<0.01),M细胞由-3.726±1.051(n=11)增至-17.992±4.159(P<0.01),心内膜下心肌细胞用药前后变化(-2.714±0.879,-2.987±1.012)差异无统计学意义(P>0.05)。结论ICa.L在犬右心室肌的不同细胞(外膜下心肌细胞、M细胞、内膜下心肌细胞)存在不均一性,β-肾上腺能激动剂可以减小右心室外膜下心肌细胞和M细胞动作电位复极1期末离子流的外向转移。     

犬左右心室肌细胞L型钙电流不均一性的研究

目的测定犬左、右心室3层心肌细胞上参与离子流平衡的内向电流L型钙电流(ICa.L)的特性。方法经酶解分离获得犬左、右心室外膜下细胞、M细胞和内膜下细胞,应用全细胞膜片钳技术,记录并比较不同部位心肌细胞的ICa.L,分析电流-电压曲线。结果ICa.L的峰值电流密度(pA/pF)在右心室外膜下细胞、M细胞和内膜下细胞分别为:-4.896±1.907(n=31),-3.406±0.904(n=37)和-2.788±0.756(n=33),心外膜下心肌细胞与M细胞比较,差异有统计学意义(P<0.05);在左心室分别为:-3.824±1.201(n=18),-4.854±1.485(n=20)和-2.988±1.082(n=17),3者之间两两比较,差异有统计学意义(P<0.05)。ICa.L的峰值电流密度在右心室心外膜下心肌细胞大于左心室(P<0.05),而右心室M细胞小于左心室(P<0.01),心内膜下心肌细胞左、右心室之间差异无统计学意义(P>0.05)。结论ICa.L在犬左、右心室肌的不同细胞(外膜下心肌细胞、M细胞、内膜下心肌细胞)存在不均一性,导致右心室跨室壁的电不均一性较左心室明显。     

去甲肾上腺素对豚鼠缺血及再灌注性室颤的作用

目的 :探讨去甲肾上腺素 (NE)在缺血性室颤及缺血 /再灌注性室颤发生中的作用及机制。方法 :运用高效液相色谱 电化学法测定离体豚鼠缺血性室颤心脏 (n =2 4 )及缺血 /再灌注性室颤心脏 (n =2 6 )流出液中NE的含量 ,并与未发生室颤心脏 (n =2 9)相比较 ,用全细胞膜片钳记录技术检测NE对缺氧豚鼠心肌细胞 (n =8)钙通道电流 (ICa)的影响。结果 :发生缺血性室颤心脏及缺血 /再灌注性室颤心脏NE释放量明显高于未发生室颤心脏 (P <0 0 1) ;NE可明显增加缺血及缺血 /再灌注心肌细胞ICa(P <0 0 1,P <0 0 5 )。结论 :NE增加心肌细胞ICa为其致缺血及缺血 /再灌注性室颤的机制之一。     

血管紧张素Ⅱ1型受体经蛋白激酶C调节L型钙流

目的　以往的研究表明血管紧张素Ⅱ (AngⅡ )对心室肌细胞L型钙流 (ICa,L)的影响及作用机制尚有争议。本文应用AngⅡ 1型受体 (AT1)阻滞剂Losartan和蛋白激酶C(PKC)抑制剂H 7来研究AngⅡ对单个豚鼠心室肌细胞ICa ,L的作用及机制。方法　应用膜片钳技术研究AngⅡ对单个豚鼠心室肌细胞ICa ,L影响的细胞机制。结果　结果表明 ,在膜片钳全细胞记录模式 ,AngⅡ刺激ICa ,L,呈浓度依赖性 ,最大作用浓度为 10 0nmol/L (n =9)。30nmol/L的AngⅡ使ICa ,L峰电流由 11 3± 0 6pA/pF增大为 15 3± 0 6pA/pF( 10mV ,n =9,P <0 0 5)。 10 0nmol/L的Losartan本身对ICa ,L无影响 ,但可抑制AngⅡ对ICa,L的作用。AngⅡ对ICa,L的作用也能被 2 0nmol/L的H 7所抑制 ,而H 7本身对ICa,L无影响。结论　以上结果提示AngⅡ经AngⅡ 1型受体刺激ICa,L,这一作用经由PKC介导而实现。     

雌激素对大鼠心肌细胞钙的调控作用研究

目的　观察雌激素对大鼠心肌细胞钙转运的调控作用 .方法　应用膜片钳全细胞记录方法观察 L型钙通道电流 (ICa.L) ,共聚焦显微镜系统分析细胞内钙浓度 ([Ca2 +]i)的变化 .结果　应用 10 μmol· L- 1和 30 μmol· L- 1的 17β- Estradiol,分别使 ICa.L抑制到正常的 (4 8.1± 4.5 ) %和 (2 9.3± 5 .2 ) % (n=5 ,P<0 .0 1) . 10μmol· L- 1 的 17β- Estradiol可升高静息的培养心肌细胞[Ca2 +]i,荧光强度 (FI)值由 5 4.2± 13.6增加到 86 .5± 15 .3(n=6 ,P<0 .0 1) ;而对具有自发收缩的心肌细胞钙瞬变幅度有明显的抑制作用 ,由给…     

模拟失重大鼠心肌细胞I_(Ca)对β-受体激动剂的反应性

目的　研究模拟失重大鼠心肌细胞钙电流 (ICa)对β-肾上腺素受体激动剂的反应性变化 .方法　用尾部悬吊大鼠模型模拟失重 (4 wk) ,胶原酶分离心肌细胞 ,用膜片钳全细胞方式记录单个心肌细胞的膜电容和 ICa等 .给予β-肾上腺素受体激动剂 (异丙肾上腺素 ) ,记录观察 ICa的反应性 .结果　对照组与悬吊组的 ICa密度无明显差别 (P>0 .0 5 ) ,ICa电流密度 -电压曲线基本一致 ;加入异丙肾上腺素后 ,两组 ICa密度均明显增大 ,但是两组的增加幅度不同 ,悬吊组的增加幅度 (84.3± 8.2 ) %明显小于对照组 (10 9.3± 7.9) % (P<0 .0 5 ) .结论　模拟失重 4wk大鼠心肌细胞的 ICa密度无明显改变 ;模拟失重后心肌细胞 ICa对 β-肾上腺素受体激动剂的反应性降低     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…