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1.
MMP—2,MMP—9,TIMP—1和TIMP—2在肝癌中原位表达   总被引:2,自引:0,他引:2  
OBJECTIVE: To explore the expression and clinical prognostic significance of matrix metalloproteinase-2 mRNA (MMP-2 mRNA), tissue inhibitor of matrix metalloproteinase-2 mRNA (TIMP-2 mRNA), matrix metalloproteinase-2 protein (MMP-2), matrix metalloproteinase-9 protein (MMP-9), tissue inhibitor of matrix metalloproteinase-1 protein (TIMP-1), and tissue inhibitor of matrix metalloproteinase-2 protein (TIMP-2) in the hepatocellular carcinomas (HCCs). METHODS: Fifty-six specimens of HCCs from 56 patients, who were followed-up, were investigated by in situ hybridization with specific probes for MMP-2, TIMP-2, and by immunohistochemistry with anti-MMP-2, MMP-9 and anti-TIMP-1, TIMP-2 monoclonal antibody. We analyzed the data with chi-square test, spearmans correlation analysis, monovariate Kaplan-Meier plot and multivariate Cox regression analysis. RESULTS: 1. The positive expression of MMP-2mRNA, TIMP-2mRNA, MMP-2 protein, MMP-9 protein, TIMP-1 protein and TIMP-2 protein in the 56 HCCs cases were 48 (85.7%), 35 (62.5%), 44 (78.6%), 41 (73.2%), 30 (53.6%), and 38 (68%), respectively. 2. We found over-expression of MMP-2 mRNA, MMP-2 protein, and MMP-9 protein, but low expression of TIMP-1 protein in the 56 cases of HCCs (P < 0.01, P < 0.05). 3. There was a positive association between TIMP-2mRNA and TIMP-2 protein expression, and between MMP-2 mRNA and MMP-2 protein in HCCs, respectively (r = 0.316, P < 0.05; r = 0.356, P < 0.05). 4. Over-expression of MMP-2 mRNA was positively correlated to the tumor size and TNM classification (r = 0.441, P < 0.001; r = 0.340, P < 0.05), and MMP-9 protein was related to shortened survival (P < 0.05). 5. In both monovariate Kaplan-Meir plot and multivariate Cox regression analysis, the expression of MMP-2 protein and MMP-9 protein were linked to unfavorable prognosis. These results were further confirmed by multivariate analysis in which MMP-2 protein and MMP-9 protein emerged as independent prognostic factors for poor survival regardless of the age, tumor size, tumor grades, TNM classification and expression of MMP-2mRNA, TIMP-2mRNA, TIMP-1 protein and TIMP-2 protein. The hazard ratios of expression of MMP-2 protein and MMP-9 protein were 3.875 and 4.528, respectively. CONCLUSION: The over-expression of MMP-2mRNA, MMP-2 protein and MMP-9 protein and the imbalance between MMP-2 and TIMP-2 play pivotal roles in the degradation of excellular matrix of HCCs. MMP-2 and MMP-9 immunoreactive protein have been closely related to a shortened survival independent of major prognostic indicators in the primary HCC and increase the risk of the patients after the operation.  相似文献   

2.
乙型肝炎病毒(HBV)表面抗原(HBsAg)基因组分别编码S、前S_1和前S_2 3种蛋白,从而组成HBsAg的主要蛋白,中分子蛋白和大分子蛋白。我们现将94份HBsAg阳性血清在检测乙肝血清标志物的同时,检测了HBV-Pre-S_1以了解分析其相互关系及其  相似文献   

3.
5—Br—PADAP光度法测定粮食中微量铜   总被引:1,自引:0,他引:1  
目的:建立简便、无毒、灵敏、准确地测定粮食中微量铜的方法。方法:2- (5- 溴- 2-吡啶偶氮)- 5- 二乙氨基苯酚(5- BrPADAP)光度法。结果:铜含量在0.00~10.00μg 范围内符合比尔定律,Y= 2.349×10- 3+ 0.05148X,r= 0.9999;用本法测定了6 个样品,回收率为90.2% ~106.0% ,平均回收率为97.8% ;日间重复试验(7 次),标准差为0.0107~0.0215m g/kg,相对标准偏差为0.59% ~1.29% 。结论:本法无毒、简便、灵敏,适用于粮食中微量铜的测定。  相似文献   

4.
红花 Carthamus tinctorius L.为菊科 1年生草本植物红花的干燥花 ,是一种常用的活血化瘀药 ,具有活血通络 ,祛瘀止痛之功效。从红花中分离得到的6-羟基山柰酚 - 3- O-葡萄糖苷 [1] ,经药效学试验证明 ,具有抑制血小板聚集 ,对抗去甲肾上腺素引起的血管平滑肌收缩等作用。本文用 RP- HPLC测定了红花中 6-羟基山柰酚 - 3- O-葡萄糖苷的含量。方法简便 ,快速 ,灵敏 ,为控制红花药材的质量 ,提供了一个良好的方法。1 仪器和药品HP 1 1 0 0型高效液相色谱仪 Chemstation工作站 ;对照品 6-羟基山柰酚 - 3- O-葡萄糖苷由红花中分离提纯得到…  相似文献   

5.
采用失重法、电化学极化曲线和电化学阻抗谱考察了0.5mol/L HCl溶液中2-巯基苯并咪唑(MBI)和1-苯基-5-疏基-四氮唑(PMTA)对铜的缓蚀作用。MBI和PMTA的加入使铜的自腐蚀电位正移,对铜电极的阳极腐蚀过程存在抑制作用,两者复配使用增强了对铜电极的阳极和阴极电化学过程的抑制作用,提高了铜电极膜电阻,从而具有较好的缓蚀协同效果。  相似文献   

6.
大肠癌中C—erbB—2基因扩增的研究   总被引:1,自引:0,他引:1  
应用差异PCR技术研究了大肠癌中C-erbB-2基因的扩增情况。结果发现,大肠癌组织中C-erbB-2基因存在明显扩增,该基因的扩增与大肠癌组织学分级、远处转移之间均有非常显著的关联。大肠癌中随着癌组织学分级程度的加重,C-erbB-2基因扩增逐渐向高、低拷贝转移,说明,C-erbB-2基因扩增在一定程度上反映着大肠癌分化状态和生物学行为,可作为大肠癌患者预后的重要参数,并可指导临床治疗。  相似文献   

7.
8.
应用免疫组化技术(ABC法),对94例胃癌及癌旁组织中表皮生长因受体(EGFR)和C-erbB-2的表达进行了研究,结果显示:(1)EGFR和C-erbB-2在正常胃粘膜中均无表达,而在胃癌中的表达率分别为40.4%,和46.8%,癌旁组织及新生血管中EGFR有阳性表达,C-erbB-2表达只限于癌灶,癌旁组织均为阴性;(2)EGFR和C-erbB-2的表达与患者的性别,年龄和肿瘤大小无关,而与肿  相似文献   

9.
目的 寻找有β-受体阻滞活性,且高效低毒的化合物。方法 以β-受体阻滞剂咔唑洛尔为先导化合物,根据药物设计中的结构拼合原理,对其丙醇胺侧链进行结构修饰。设计并合成了3个1-(9H-咔唑-4-氧)-3-取代氨基-2-丙醇类化合物V11-V13及其6个相应的酯化物VI1-VI6。结果和讨论 所合成的目的物均未见文献报道,结构经红外光谱,核磁共振氢谱,质谱,元素分析或高分辨质谱确证,初步药理筛选结果显示,9个化合物均能够不同程度地拮抗异丙肾上腺素引起的心动过速,其中化合物V12,Ⅵ3,Ⅵ4的活性与先导化合物相当。  相似文献   

10.
恶性淋巴瘤组织中FAS—FASL及Bcl—2的表达   总被引:1,自引:0,他引:1  
1 对象和方法1 1 对象  19931996年在我院确诊并甲醛固定 ,石蜡包埋病理活检组织的恶性淋巴瘤 (malignantlymphoma ,ML)患者 5 6例 ,其中男 42例 ,女 14例 ,平均年龄 43 (872 )。5 6例中非霍奇金淋巴瘤 (non Hodgkin′sLymphoma ,NHL)48例 (B细胞型 2 2例 ,T细胞 12例 ,未确定者 14例 ) ,霍奇金病 (Hodgkin′sdisease ,HD) 8例。淋巴结反应性增生 (reac tivehyperplasiaoflymphnode ,LNRH) 10例作为对照组。所有标本均制成 4μm厚连续…  相似文献   

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