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1.
目的:探讨血管紧张素转换酶(ACE)基因多态性在结直肠癌(CRC)发病中的作用。方法:入组120例CRC患者,采用聚合酶链反应(PCR)检测患者外周血中ACE基因多态性和等位基因分布频率,研究ACE基因缺失型(DD型)、II型和插入型(ID型)等3种基因型及I/D等位基因在CRC患者外周血中的分布频率,并与120例正常人检测结果进行对比。结果:CRC患者外周血标本中存在ACE基因插入/缺失(I/D)多态性分布,DD型、ID型和II型3种基因型的频率分别为15.00%,50.00%,35.00%,I/D等位基因分布频率分别为60.00%及40.00%;对照组的分布频率分别为16.67%,53.33%,30.00%,I/D等位基因分布频率分别为56.67%及43.33%,两组间3种基因型的分布频率无显著性差异(P>0.05)、I/D等位基因分布频率无显著差异(P>0.05)。分层分析表明,D等位基因变异与CRC低分化和远处转移相关(P<0.01及P<0.05)。结论:ACE基因I/D多态性与CRC存在一定关系,其中D等位基因异常与CRC低分化和易转移可能有关。  相似文献   

2.
目的:探讨2型糖尿病(DM)合并冠心病(CHD)与血管紧张素转化酶I(ACE)基因插入/缺失(I/D)多态性的相关性及其各基因型血脂代谢情况. 方法:采用多聚酶链反应(PCR)技术,对32例广西地区汉族2型DM合并CHD患者,88例2型DM无合并CHD患者及100名汉族正常对照的ACE基因I/D多态性及血脂进行检测.结果:2型DM合并CHD组ACE基因D等位基因及ID基因型发生频率高于正常对照组及无合并CHD组(P<0.05);I等位基因及II基因型频率低于正常对照组及无合并CHD(P<0.05).2型DM合并CHD组ID基因型组甘油三酯(TG)及总胆固醇(TC)高于正常对照组、DD基因型组及II基因型组,高密度脂蛋白胆固醇(HDL-C)低于正常对照组,低密度脂蛋白胆固醇(LDL-C)高于正常对照组(P<0.05);2型DM合并CHD DD基因型组TG、LDL-C及TC高于正常对照组(P<0.05).结论:①广西地区汉族2型DM合并CHD与ACE基因I/D多态性有关.②D等位基因及ID基因型可能为2型DM合并CHD的易感基因,I等位基因及II基因型可能为2型DM合并CHD的保护基因.③广西地区汉族2型DM合并CHD的 ID基因型者及DD基因型者易合并高甘油三酯血症及高胆固醇血症.  相似文献   

3.
目的:探讨血管紧张素转化酶基因I/D多态性与青海省贵德地区汉族冠心病(CHD)患者之间的相关关系。方法:冠心病患者89例和健康汉族75例,应用聚合酶链反应(PCR)技术检测两组人群血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性,观察II、ID、DD基因型频率。结果:通过冠心病组和正常对照组的ACE基因I/D多态性的比较发现,冠心病组DD、ID和II基因型频率分别为33.7%、46.1%、20.2%,D、I等位基因频率分别为56.7%、43.3%;健康对照组分别为20.0%、44.0%、36.0%和42.0%、58.0%。冠心病组血管紧张素转化酶基因型及等位基因频率与对照组相比有显著性差异(P<0.05)。ACE的DD基因型频率和D等位基因频率在冠心病组中显著增加(P<0.05)。结论:贵德地区冠心病与ACE基因多态性有一定相关性,ACE、DD基因型可能是冠心病的危险因子。  相似文献   

4.
目的研究血管紧张素转换酶(ACE)基因多态性与非胰岛素依赖型糖尿病并发冠心病的关系.方法应用PCR方法分析149例患者及100例正常对照的ACE基因型.结果①ACE基因型及等位基因构成比,正常对照组与患者组差异无统计学意义;②DD型及D等位基因频率并发冠心病组显著高于无冠心病组(P<0.01).结论:ACE基因I/D多态性与并发冠心病有关.  相似文献   

5.
食管癌患者血管紧张素转换酶基因多态性   总被引:1,自引:0,他引:1  
目的检测食管癌患者血管紧张素转换酶(ACE)基因多态性,评估ACE基因与食管癌的关系。方法选择50例食管癌患者为研究组,采用PCR检测ACE基因,观察缺失纯合子型(DD型)、缺失与插入纯合子型(ID型)和插入纯合子型(Ⅱ型)3种基因型多态性分布频率,及计算I/D等位基因比率,50例同年龄段健康人群作为对照。结果 50例食管癌患者ACE基因呈插入/缺失(I/D)多态性分布,DD型、ID型和Ⅱ型基因占比分别为44.0%,22.0%,34.0%,I/D等位基因分布频率分别为34.0%和66.0%,对照组DD型、ID型和Ⅱ型3种基因型的占比分别为18.0%、52.0%、30.00%,I/D等位基因分布频率分别为30.0%和70.0%,两组间DD型、ID型基因的分布频率差异有统计学意义(P<0.01)、I/D等位基因分布频率差异无统计学意义。结论食管癌患者存在ACE基因I/D多态性,DD型基因分布较正常对照组显著升高,ID型显著降低,提示DD基因型高表达与食管癌发病有一定联系。  相似文献   

6.
目的探讨血管紧张素转化酶(ACE)基因型及其血清活性与原发性高血压(EH)的关系.方法以人类基因组DNA为模板,应用聚合酶链式反应(PCR)技术检测120例EH组和93例正常对照组ACE基因第16内含子的插入/缺失(I/D)多态性,并按性别分组计算各组基因型和等住基因频率,另外用比色法测量各研究对象的ACE血清活性.结果男性EH组DD基因型和D等位基因频率均显著高于对照组(均P<0.05).女性EH组DD基因型频率和D等位基因频率与对照组比较差异无统计学意义(P>0.05);在EH组中ACE活性与对照组比较差异无统计学意义(P>0.05);ACE血清活性在ACE基因DD,ID和Ⅱ型间有显著性差异(P<0.05).结论EH与ACE基因I/D多态性有显著相关性,特别是男性,ACE基因DD基因型可能在EH的发生发展过程中是重要的危险因素之一.ACE血清活性与ACE基因I/D多态性有关.  相似文献   

7.
抑郁症患者ACE和AGT基因多态性研究   总被引:1,自引:0,他引:1  
王佳  谢健  陈岳明 《浙江医学》2010,32(12):1750-1753
目的 探讨血管紧张素转换酶(ACE)基因插入或缺失(I/D)多态性与血管紧张素原(AGT)基因M235T多态性和抑郁症之间的关联.方法 运用聚合酶链反应结合限制性片段长度多态性检测技术(RFLP-PCR)检测150例抑郁症患者(研究组)和180例健康体检者(对照组)的ACE I/D、AGT M235T等位基因频率和基因型.同时用酶联免疫吸附法(ELISA)检测两组患者的血浆ACE含量.结果 研究组和对照组的ACE I/D位点等位基因频率和基因型差异均有统计学意义(P<0.05),DD基因型的抑郁症患病风险升高(OR=2.06,95%CI:1.29~3.85).对照组血浆ACE含量明显低于研究组,II、ID、DD基因型的血浆ACE含量不同,以DD基因型的ACE含量最高.差异均有统计学意义(均P<0.05).两组间AGT M235T等位基因频率和基因型的差异无统计学意义(P>0.05).结论 ACE基因I/D多态性和抑郁症有关,DD基因型个体的抑郁症患病风险增高.  相似文献   

8.
内蒙古地区妊娠高血压患者ACE基因多态性研究   总被引:1,自引:0,他引:1  
目的:检测内蒙古地区妊娠高血压患者ACE基因Ⅰ/D多态性与妊娠高血压的相关性。方法:采用PCR-SSP(序列特异性引物)检测80例正常妊娠组和40例妊娠高血压组ACE基因I/D基因型频率分布。结果:妊娠高血压组患者DD基因型频率高于正常妊娠组;妊娠高血压组D型等位基因频率高于对照组。结论:内蒙古地区汉族妊娠期高血压疾病的发病与ACE基因I/D多态性有相关性。  相似文献   

9.
目的 研究云南省晋宁县彝族人群ACE基因插入/缺失(I/D)多态性分布的情况.方法 采用聚合酶链式反应(PCR)技术,检测居住在云南省晋宁县129例彝族健康人的ACE基因I/D多态性,计算DD、ID、II基因型频率和D、I等位基因频率.结果 云南省晋宁县彝族ACE基因的DD、ID、II基因型频率分别为17.1%、42.6%和40.3%,D、I等位基因频率分别为38.4%和61.6%.和不同种族人群比较,云南彝族人群ACE基因I/D多态性分布与古巴黑人、古巴白人和美国黑人差异有统计学意义(P<0.01),而与日本人接近(P>0.05).与我国其它民族比较,除云南彝族人群的II基因型频率和I等位基因频率高于维吾尔族(P<0.05)、D等位基因频率低于维吾尔族(P<0.05)外,云南彝族人群ACE基因I/D多态性分布接近汉族、黎族、哈萨克族、蒙古族和藏族(P>0.05).结论 ACE基因型频率和基因频率存在种族差异.  相似文献   

10.
血管紧张素转换酶基因多态性与冠心病关系的研究   总被引:5,自引:4,他引:1  
目的 :研究血管紧张素转换酶 (ACE)基因多态性与冠心病的关系。方法 :应用聚合酶链式反应 (PCR)检测ACE基因第 16内含子I/D多态性 ,并计算基因型和等位基因频率。结果 :在 5 1例冠心病组中ACE基因DD基因型和D等位基因频率分别是 35 %和 6 1% ,83例正常对照组中的ACE基因DD基因型和D等位基因频率分别是 16 %和 45 % ,两者相比具有显著性差异。结论 :ACE基因DD基因型可能是冠心病发生发展过程中重要的危险因素之一。  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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