赤丹丸与其组方药材的体外抑菌试验

目的：研究赤丹丸及其组方药材的体外抑菌作用。方法：采用液体培养基2倍稀释法测定赤丹丸及其组方药材的水提取物和乙醇提取物对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、白色念珠菌的体外最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。结果：赤丹丸及其组方药材的水提取物和醇提取物对白色念珠菌的抑菌作用明显弱于对其它3种菌的作用（P〈0．05），其中药材的水提取物体外抑制白色念珠菌作用相对较强（P〈0．05）；各种提取物对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌的抑菌作用也各有差异。药材水提取物对铜绿假单胞菌、白色念珠菌的抑菌作用最强（P〈0．05）；赤丹丸醇提取物对金黄色葡萄球菌、大肠埃希菌的抑菌作用最强（P〈0．05）。结论：赤丹丸及其组方药材的水提取物与醇提取物对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、白色念珠菌均具有体外抑菌作用。     

彩虹牌银离子消毒气雾剂杀菌效果的研究

目的　评价银离子消毒气雾剂的消毒效果 ;方法　按《消毒技术规范》(2 0 0 2年版 )规定进行 ;结果　该消毒剂稀释液对金黄色葡萄球菌、大肠杆菌、白色葡萄球菌杀灭对数值均≥ 5 ;有机物对该消毒剂杀菌效果无影响 ;该消毒剂对桌面上的自然菌平均杀灭对数值为 1.2 3;该消毒剂对空气中自然菌平均消亡率 92 .4 4 % ;结论　银离子消毒气雾剂能有效杀灭金黄色葡萄球菌、大肠杆菌、白色葡萄球菌 ,可用于物表消毒和室内空气消毒。     

圣洁水生物液杀菌效果与稳定性试验研究

目的 了解圣洁水生物液杀菌效果与稳定性。方法 中和剂按消毒技术规划进行，鉴定用悬液定量杀菌试验和稳定性试验。结果 6%圣洁水生物液对大肠杆菌、金黄色葡萄球菌作用3min，杀灭率达99.90%；12.5%浓度对白色念珠菌作用5min，杀灭率达99.90%，其杀菌效果受有机物影响，而54℃放置14d无明显改变。结论 圣洁水生物液对大肠杆菌、金黄色葡萄球菌及白色念珠菌均具有较好的杀菌效果，且性能稳定。     

聚维酮碘泡腾片杀菌效果测定

目的：研究聚维酮碘泡腾片对金黄色葡萄球菌、大肠杆菌和白色念珠菌的杀菌效果。方法：采用定量杀菌试验方法，用不同有效碘含量的样品溶液分别对金黄色葡萄球菌、大肠杆菌和白色念珠菌作杀菌试验。结果：用2g/L有效碘溶液对金黄色葡萄球菌、大肠杆菌作用1min，白色念珠菌作用6min;1g/L有效碘溶液对金黄色葡萄球菌作用3min、大肠杆菌作用1min，白色念珠菌作用6min;0.5g/L有效碘溶液对金黄色葡萄球菌作用3min、大肠杆菌作用3min，白色念珠菌作用12min,杀灭率均为99.99%。其杀菌作用随着有效碘含量与作用时间增加而加强，有机物对杀菌效果有轻微影响。结论：本品杀菌效果显著，是一种理想的消毒杀菌剂。     

艾叶复方消毒剂杀菌效果的实验观察

目的：观察以艾叶中草药提取复方消毒剂的杀菌效果及有机物的影响。方法：按《消毒技术规范》规定的方法分组、操作与判断试验结果。结果：作用５ｍｉｎ，可将淋球菌，大肠杆菌，金黄色葡萄球菌，绿脓杆菌，白色念珠菌杀灭９９．９９％，受有机物影响小；且该消毒剂将电话机与冰箱门把表面喷湿，可杀灭自然菌９９．８３％，结论：艾叶复方消毒剂具有良好杀菌效果。     

蜂胶与杨树胶抑菌作用的比较研究

目的探讨蜂胶与杨树胶的体外抑菌效果。方法以金黄色葡萄球菌、大肠杆菌、伤寒杆菌、枯草杆菌和白色念珠菌等几种常见菌为试验菌株,采用琼脂打孔扩散法对不同浓度蜂胶、杨树胶进行体外抑菌试验。结果蜂胶对金黄色葡萄球菌、大肠杆菌、白色念珠菌和枯草杆菌有抑制作用,抑菌圈大小与80％乙醇比较均有显著性差异（P〈0．01）;杨树胶对金黄色葡萄球菌、伤寒杆菌、白色念珠菌和枯草杆菌有抑制作用,抑菌圈大小与80％乙醇比较均有显著性差异（P〈0．01）。结论蜂胶与杨树胶均有明显的抑菌活性。     

妇康栓消毒效能和毒性观察

目的：为了解妇康栓的杀菌性能和使用安全性。方法：按1999年版卫生部《消毒技术规范》规定的方法对妇康栓杀灭金黄色葡萄球菌、大肠杆菌和白色念珠菌及毒性进行了实验研究。结果：在37℃水浴条件下。该妇康栓原液对金黄色葡萄球菌、大肠杆菌作用2min的杀灭率在99．90％以上。对白色念珠菌作用5min杀灭率在99．90％以上。有机物对其杀菌作用无明显影响，毒性检测表明无毒。对皮肤无刺激。结论：妇康栓有较好的杀灭细菌作用，可用于阴道消毒。     

青连空气消毒剂的研制与杀菌效果观察

目的:探讨自制中药空气消毒剂青连夜的消毒效果。方法:实验室对青连液单味药提取物的杀菌效果、不同浓度的杀菌效果分别进行对比:原液对病室空气自然菌消毒前后的效果、与0.5%的过氧乙酸的杀菌效果分别进行对比。方法:青连消毒剂原液对金黄色葡萄球菌、大肠杆菌、白色念珠菌10分钟杀菌率达100%。效果好与单味药提取物:对病室空气自然菌杀灭率均>92%:与过氧乙酸杀菌作用基本相同均>91%。结论:青连液是一种对人体无刺激的纯中药空气消毒剂。     

珊瑚姜油消毒液的消毒杀菌研究

目的：研究珊瑚姜油达到消毒杀菌剂的条件。方法：观察中和剂对消毒剂的中和作用以达到作为消毒剂的基本条件。采用细菌定量杀灭试验,测定珊瑚姜油消毒液对微生物的杀灭效果;采用悬液定量杀菌试验对消毒液的稳定性进行测试。结果：在（20±1）℃的条件下,用含2%甘氨酸、0.3%卵磷脂、3%吐温-80的0.03 mol/L PBS作中和剂,可有效中和金黄色葡萄球菌和白色念珠菌表面残留消毒液对受试菌的作用;珊瑚姜油消毒液对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、白色念珠菌都有很好杀灭效果,且具有很好的稳定性。结论：珊瑚姜油消毒液的消毒作用强,稳定性好。     

LED紫外线灯对足部感染指标菌的杀菌效果研究

目的观察LED紫外线灯在不同照射距离、不同照射时间条件下对金黄色葡萄球菌、白色念珠菌、黑曲霉菌的杀菌效果。方法采用载体定量杀菌试验,菌片经LED紫外线灯照射后,将细菌洗下制成细菌悬液后进行活菌培养计数,比较杀菌效果。结果 0.12W LED紫外线灯在垂直照射距离为2 cm时照射3 min,对金黄色葡萄球菌、白色念珠菌、黑曲霉菌的杀灭率均为100.00%;在垂直照射距离为3 cm时照射时间为4 min,对金黄色葡萄球菌、白色念珠菌、黑曲霉菌的杀灭率均为100.00%;在垂直照射距离为4 cm、5 cm时照射时间为5 min,对金黄色葡萄球菌、白色念珠菌、黑曲霉菌的杀灭率均为100.00%。结论 0.12W LED紫外线灯可杀灭足部感染指标菌。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…