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1.
目的 研究缺氧诱导因子-1α(HIF-1α)反义寡核苷酸对人胃癌裸鼠皮下移植瘤的治疗作用.方法 用人胃癌细胞株SCG-7901建立胃癌裸鼠皮下移植瘤模型,将荷瘤裸鼠随机分为反义寡核苷酸(ASOND)组、正义寡核苷酸(SOND)组和对照组,瘤体内分别注射阳离子脂质体包裹的HIF-1α ASOND、HIF-1α SOND和空白阳离子脂质体.观察各组动物的肿瘤生长曲线,测瘤重计算抑瘤率,光学显微镜下观察肿瘤细胞形态学变化,免疫组化方法 检测各组肿瘤组织HIF-1α、血管内皮生长因子(VEGF)和微血管密度(MVD)的表达.结果 ASOND组裸鼠的肿瘤生长受到明显抑制,ASOND组瘤重与对照组比较差异有统计学意义(P<0.05);ASOND组、SOND组抑瘤率分别为47.94%、16.88%,差异有统计学意义(P<0.01);ASOND组肿瘤组织中HIF-1α、VEGF和MVD的表达低于SOND组和对照组(P<0.01).结论 肿瘤原位注射HIF-1α反义寡核苷酸治疗胃癌裸鼠皮下移植瘤能降低HIF-1α的表达,继而减少VEGF的生成和新生血管的形成,抑制肿瘤的生长.  相似文献   

2.
目的探讨乙型肝炎病毒 x 蛋白(Hepatitis B virus X protein,HBx)和缺氧诱导因子-1α(Hypoxia-inducible factor,HIF-la)在肝癌组织中的表达及关系。方法采用免疫组织化学染色方法检测78例经福马林固定、石蜡包埋的原发性肝癌组织标本中HBx 和 HIF-1α的表达;免疫荧光检测 HepG2及稳定转染 HBx 基因的 HepG2(HBx-transfected HepG2)细胞中 HIF-1α的表达。结果78例肝癌组织标本中,HBx 和 HIF-1α免疫组织化学染色阳性率分别为74.23%(58/78)和69.23%(54/78);免疫荧光检测表明:正常氧状态下,HeptG2中 HIF-1α的表达阴性而 HBx-transfected HepG2中表达阳性,主要位于细胞浆.部分位于细胞核.在缺氧状态下,HeptG2和 HBx-transfected HepG2的细胞质和细胞核均有表达.结论 HBx 及 HIF-1α在人肝细胞肝癌组织中广泛表达,二者存在明显相关(P<0.01),在正常氧状态下,HBx 可诱导 HIF-α在 HepG2细胞中表达。提示:共同表达可能对原发性肝癌细胞的形成有重要作用。  相似文献   

3.
缺氧诱导因子-1α的表达与肝癌血管生成的关系   总被引:2,自引:1,他引:2  
目的:探讨缺氧诱导因子-1α(HIF-1α)的表达与肝癌血管生成的关系及HIF-1α、血管内皮生长因子(VEGF)、微血管密度(MVD)对肝癌生物学行为的影响。方法:应用免疫组织化学法检测肝癌组织中HIF-1α、VEGF的表达,用CD34单克隆抗体标记肿瘤血管内皮细胞,计数肝癌组织中的肿瘤MVD。结果:肝癌组织中HIF-1α、VEGF的表达阳性率分别为71%、75%,MVD为41.32±9.47,明显高于正常肝组织(P<0.01),HIF-1α与VEGF的表达及MVD的变化呈显著正相关(r=0.37,P<0.05;r=0.685,P<0.001)。HIF-1α、VEGF、MVD与肝癌的淋巴转移、包膜及静脉侵犯密切相关(P<0.05)。结论:HIF-1α的表达与肝癌新生血管的生成密切相关,HIF-1α的过表达、高MVD与肝癌的不良生物学行为有关,影响肝癌的预后,可能成为预测肝癌转移复发的参考指标。  相似文献   

4.
目的探讨乙型肝炎病毒x蛋白(Hepatititis B virus X protein,HBx)和缺氧诱导因子-1α(Hypoxia-inducible factor,HIF1α)在肝癌组织中的表达及关系.方法采用免疫组织化学染色方法检测78例经福马林固定、石蜡包埋的原发性肝癌组织标本中HBx和HIF-1α的表达;免疫荧光检测HepG2及稳定转染HBx基因的HepG2(HBx-transfected HepG2)细胞中HIF-1α的表达.结果78例肝癌组织标本中,HBx和HIF-1α免疫组织化学染色阳性率分别为74.23%(58/78)和69.23%(54/78);免疫荧光检测表明:正常氧状态下,HeptG2中HIF-1α的表达阴性而HBx-transfected HepG2中表达阳性,主要位于细胞浆,部分位于细胞核.在缺氧状态下,HeptG2和HBx-transfected HepG2的细胞质和细胞核均有表达.结论HBx及HIF-1α在人肝细胞肝癌组织中广泛表达,二者存在明显相关(P<0.01),在正常氧状态下,HBx可诱导HIF-α在HepG2细胞中表达.提示:共同表达可能对原发性肝癌细胞的形成有重要作用.  相似文献   

5.
王燕  吴强 《现代医学》2009,37(4):270-273
目的 探讨缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)在宫颈癌中的表达及其与微血管生成的关系.方法 应用免疫组织化学技术分别检测43例宫颈癌、32例宫颈上皮内瘤样病变(CIN)和43例正常宫颈组织中的HIF-1α、VEGF的表达及微血管密度(MVD).结果 HIF-1α、VEGF、MVD在正常宫颈组织、CIN、宫颈癌中的阳性表达率均依次升高,3组之间比较均有显著性差异(均P<0.01);HIF-1α的表达与VEGF呈正相关(P<0.05),VEGF的表达与MVD也呈正相关(P<0.05).结论 HIF-1α、VEGF及MVD在宫颈癌的发生、发展中起重要作用,HIF-1α在宫颈癌组织中呈高表达,并通过VEGF表达促进肿瘤血管形成.HIF-1α可能成为治疗宫颈癌新的靶基因.  相似文献   

6.
目的揭示在常氧和缺氧状态下,缺氧诱导因子-1α(HIF-1α)在HepG2中的表达及HBx对HIF-1α表达的调节。方法 对肝癌细胞HepG2及HBx转染的HepG2分别进行常氧和缺氧培养,其中缺氧状态用1%O2、5%CO2和94%Nz模拟,缺氧时间分别为1h,2h,4h,8h,16h,32h。采用免疫印迹检测HIF-1α表达。结果 在常氧状态下,HepG2细胞中HIF-α几乎无表达而HBx转染的HepG2明显表达。二者在缺氧1h开始均表达,8h达到高峰,16h后逐渐下降,测量二者缺氧8h时的表达,发现HBx转染的HepG2细胞中HIF-1α的表达增高。结论 在常氧状态下,HBx可诱导HIF-1α在HepG2细胞中表达。在缺氧状态下,HIF-α在HepG2细胞中表达与缺氧的时间相关且HBx可增强缺氧状态下HIF-1α在HepG2细胞中表达。提示HBx可能通过HIF-1α通路在肝癌的形成过程中发挥重要的作用。  相似文献   

7.
目的 探讨靶向肝癌HepG2细胞VEGF基因siRNA表达载体对人肝癌细胞株裸鼠成瘤的抑制作用.方法 将设计并合成构建好的靶向肝癌HepG2细胞VEGF基因的psuper.retro.neo-VEGF-siRNA表达载体转入HepG2细胞,通过G418抗性筛选出稳定株(HepG2/psuper.retro.neo-VEGF-siRNA,实验组),同时设对照组(HepG2/psuper.retro.neo组)和空白组(HepG2组).分别移植BALA/c裸鼠成瘤,计算各组鼠成瘤潜伏期和接种20 d后瘤重,Western-blotting检测肿瘤组织中VEGF的表达变化,免疫组织化学SP法检测瘤组织内微血管密度(MVD).结果 实验组、对照组和空白组裸鼠成瘤潜伏期分别为(9.2±1.2)、(3.9±0.7)和(3.8±0.9)d;接种20 d后3组平均瘤重分别为(194±57)、(566±86)和(626±96)g;瘤组织VEGF蛋白表达水平分别为(0.075±0.012)、(0.198±0.009)和(0.205±0.008);MVD分别为(13.6±2.8)、(34.3±2.9)和(35.3±3.5),实验组与对照组和空白组比较差异有统计学意义(P<0.05).结论 合成和构建的靶向人肝癌HepG2VEGF基因干扰质粒具有抑制人肝癌细胞裸鼠成瘤和血管生成的作用.  相似文献   

8.
目的:研究缺氧诱导因子1α(HIF-1α)及其靶基因血管内皮细胞生长因子(VEGF)在子痫前期患者胎盘组织中的表达和相关性,探讨HIF-1α、VEGF在子痫前期发病机制中的作用。方法:采用免疫组化链霉素抗生物素蛋白-过氧化酶连接法(SP)法对20例子痫前期患者(子痫前期组)和15例正常妊娠妇女(对照组)胎盘组织中HIF-1α、VEGF蛋白表达进行半定量分析;应用RT-PCR技术检测两组胎盘组织中HIF-1α、VEGFmRNA的水平并进行相关性分析。结果:(1)免疫组化结果显示,子痫前期组HIF-1α蛋白表达明显增强,其中( )阳性表达率分别为45%,显著高于对照组13.3%(P<0.05),而VEGF蛋白表达明显减弱,其中( )阳性表达率为15%,显著低于对照组(P<0.01)。(2)子痫前期组HIF-1αmRNA的水平为(0.604±0.013),显著高于对照组的(0.208±0.007),两组比较,差异有统计学意义(P<0.05),VEGF mRNA表达水平虽有升高,但与对照组相比较,差异无统计学意义(P>0.05)。结论:HIF-1α在子痫前期胎盘组织中的表达明显升高,可能通过调节靶基因VEGF的转录影响滋养细胞的浸润功能和胎盘血管重铸,在子痫前期的发病过程中发挥重要作用。  相似文献   

9.
目的探讨乙肝病毒X蛋白(hepatitis B virus X protein,HBx)与缺氧诱导因子-1α(hypoxia-inducible factor,HIF-1α)在乙肝相关性肝细胞肝癌(hepatocellular carcinoma,HCC)组织血管生成及转移中的作用。方法选择84例乙肝相关性肝癌组织和22例非乙肝相关性肝癌组织,免疫组化法检测HBx、HIF-1α及血管内皮细胞表面抗原(CD34)表达,光镜下记录微血管计数(microvessel density,MVD)。结果 84例乙肝相关性肝癌组织中HBx阳性表达率为73.81%(62/84)。62例HBx阳性表达组中的HIF-1α阳性率为69.35%(43/62),明显高于HBx阴性组40.91%(9/22)和非乙肝相关性肝癌组36.36%(8/22)(P<0.05)。HBx阴性表达的乙肝相关性肝癌组和非乙肝相关性肝癌组中HIF-1α阳性表达无统计学意义(P>0.05)。HBx和HIF-1α的表达呈正相关(rs=0.573,P<0.01);HBx和HIF-1α在高分化肿瘤组织中的表达高于低分化组织(P<0.05),且转移组表达高于无转移组;HBx阳性表达组平均MVD值明显高于HBx阴性组和非乙肝相关性肝癌组(P<0.01),有转移组MVD高于无转移组(P<0.01);有门脉侵犯组高于非侵犯组(P<0.05)。结论 HBx和HIF-1α广泛表达于乙肝相关性肝癌组织中,二者呈正相关;HBx可能通过上调HIF-1α的表达在乙肝相关性肝癌组织血管生成及转移中起促进作用。  相似文献   

10.
目的探讨肝细胞癌组织中缺氧诱导因子1α(HIF-1α)和血管内皮生长因子(VEGF)的表达及其与肝细胞癌分化程度、恶性程度及侵袭性的关系。方法根据艾德曼森(Edmondson)分级对肝癌组织进行分级,采用免疫组化的方法检测23例肝细胞癌组织、癌旁组织和23例正常肝组织中HIF-1α和VEGF的表达,结果(1)3组HIF-1α、VEGF表达程度比较,差异均有统计学意义(P<0.05)。其中正常肝组织组HIF-1α、VEGF表达程度与癌旁组织、肝癌组织组比较,差异均有统计学意义(P<0.05);癌旁组织组HIF-1α与肝癌组织组比较,差异无统计学意义(P>0.05);癌旁组织组VEGF表达程度与肝癌组织组比较,差异有统计学意义(P<0.01)。(2)高侵袭性与低侵袭性肝癌组织中HIF-1α、VEGF的表达程度比较,差异均无统计学意义(P>0.05)。(3)肝癌组织中HIF-1α、VEGF的表达程度与Edmondson分级间均无等级相关关系(rs分别为0.225及0.162,P>0.05)。(4)肝癌组织中HIF-1α的表达与VEGF表达间有等级相关关系(rs=0.597,P<0.05)。结论 HIF-1α和VEG...  相似文献   

11.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

12.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

13.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

14.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

15.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

16.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.  相似文献   

19.
Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.  相似文献   

20.
ANTI-PROLIFERATION EFFECT OF ORIDONIN ON HL-60 CELLS AND ITS MECHANISM   总被引:4,自引:0,他引:4  
Objcetive To investigate the anti-proliferation effect of oridonin on leukemic HL-60 cells and its mechanism. Methods HL-60 cells in vitro in culture medium were given different concentrations of oridonin. The inhibitory rate of cells were measured by microculture tetrazolium (MTT) assay, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the activity of telomerase was detected using telomere repeat amplification protocol (TRAP) PCR-ELISA before and after apoptosis occurred. Results Oridonin could decrease telomerase activity, inhibit growth of HL-60 cells, and cause apoptosis significantly. The suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by hoechst 33258 fluorescence staining especially after cells were treated 48-60 hours by oridonin. Conclusions Oridonin has apparent anti-proliferation and apoptotic effects on HL-60 cells in vitro, decreasing telomerase activity of HL-60 cells may be one of its most important mechanisms. These results will provide strong laboratory evidence of oridonin for clinical treatment of acute leukemia.  相似文献   

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