人肺腺癌细胞A549转导HSV1—TK基因体内外表达研究

目的：观察Ａ５４９细胞转导ＴＫ基因在体外和体内的表达。方法：将已构建的 转录病毒表达载体ＰＬＸＳＮ－ＴＫ用电转化法转化Ａ５４９细胞，原位杂交检测ＴＫｍＲＮＡ在Ａ５４９－ＴＫ和由它接种裸鼠形成肿瘤组织中的表达。斑点杂交检测外源基因在细胞中整合，并体外观察Ａ５４９－ＴＫ对ＧＣＶ的敏感性。结果：原发杂交表明Ａ５４９－ＴＫ细胞及其形成肿瘤组织ＴＫｍＲＮＡ表达阳性，对照细胞无表达，斑占杂交证明Ａ５４９－ＴＫ     

HSV1—TK基因转导入肺腺癌细胞A549体内外表达的研究

目的：观察ＴＫ基因转导Ａ４９细胞后在体外和体内的表达。方法：将已构建的逆转录病毒表达载体ＰＬＸＳＮ－ＴＫ用电穿孔转化Ａ５４９细胞，原位杂交检测ＴＫｍＲＮＡ在Ａ５４９－ＴＫ细胞和由它接种裸鼠形成肿瘤组织中的表达。斑点杂交检测外泊基因在细胞中整合，并体外观察Ａ５４９－ＴＫ细胞对ＧＣＶ的敏感性。结果：原位杂交表明Ａ５４９－ＴＫ细胞及由其所形成的肿瘤组织中ＴＫｍＲＮＡ表达阳性，对照细胞无表达，斑点杂交证明     

HSV1—TK／GCV系统对人肺腺癌细胞S549生物学特性的影响

目的 观察转ＴＫ基因肺癌细胞对丙氧鸟苷（ＧＣＶ）的敏感性及ＴＫ／ＧＣＶ系统杀灭肿瘤机制。方法 观察转ＴＫ基因，转空载体Ａ５４９细胞（下分别简称Ａ５４９－ＴＫ、Ａ５４９－ｐＬＸＳＮ）和Ａ５４９细胞形态学改变、生长增殖特性。ＧＣＶ对３种细胞生长抑制（ＭＴＴ法）。电镜、流式细胞仪观察Ａ５４９－ＴＫ、Ａ５４９细胞经５０μｍｏｌ／Ｌ ＧＣＶ作用３ｄ后细胞凋亡。结果 转基因细胞变为不规则，呈多角型，透亮度下降     

香菇多糖对正常小鼠以及免疫抑制小鼠免疫功能的影响

目的：研究香菇多糖对正常小鼠以及免疫抑制小鼠免疫功能的影响,探讨香菇多糖对小鼠免疫功能的影响及其量效相关性。方法：分别检测在3个不同剂量下香菇多糖对正常小鼠以及免疫抑制小鼠红细胞C3b受体(RBC-C3bR)花环形成率及红细胞免疫复合物(RBC-CIC)花环形成率、巨噬细胞吞噬功能、抗体生成实验、免疫器官重量及淋巴细胞转化实验等指标的影响。结果：香菇多糖可使正常小鼠以及免疫抑制小鼠红细胞C3b受体花环及红细胞免疫复合物花环形成均有不同程度的升高;巨噬细胞吞噬活性增强;抗体效价升高;免疫器官重量增加;淋巴细胞转化OD值增加,且以高剂量为佳。结论：香菇多糖能增强小鼠非特异性免疫、体液免疫及红细胞免疫能力,在一定的浓度范围内存在量效正相美性．并且对免疫抑制小鼠效果为佳．     

人肺腺癌A549多重抗药细胞株生物学特性变化

目的；探讨人肺腺癌Ａ５４９多重抗药细胞株生物学特性变化，方法：采用阿霉素（ＡＤＭ）浓度递增的方法诱导人肺腺癌Ａ５４９细胞系（Ａ５４９／Ｐ）在体外持续培养６个月，获得了具有多重抗药的Ａ５４９／Ｒ２细胞，该细胞能在０．２μｇ／ｍｌＡＤＭ下持续增殖，通过光镜和电镜观察其增殖及形态结构变化，结果：Ａ５４９／Ｒ２细胞由巨大细胞增殖而来，其微绒毛，线粒体及分泌泡明显增加，有进一步分化趋向，结论：Ａ５４９／Ｒ２     

丁硫氨酸亚砜胺逆转多重抗药细胞的抗性作用及其作用机理的研究

应用阿霉素诱导的人肺腺癌Ａ５４９多重抗药细胞Ａ５４９／Ｒ２，对丁硫氨酸亚砜胺（ＢＳＯ）逆转多重抗药细胞的抗性作用及其作用机理进行了初步探讨。研究发现：非细胞抑制剂量ＢＳＯ能使Ａ５４９／Ｒ２细胞的ＩＣ５０值显著下降（Ｐ＜０．０１）；大剂量ＢＳＯ能直接抑制癌细胞增殖，且对抗药细胞更有效；Ａ５４９／Ｒ２细胞内总体谷胱甘肽（ＧＳＨ）含量和谷胱甘肽Ｓ－转移酶（ＧＳＴ）活性均非常显著地高于其敏感细胞（Ａ５４９／Ｐ）水平（Ｐ＜０．０１）；ＢＳＯ能使Ａ５４９／Ｒ２细胞内总ＧＳＨ含量和ＧＳＴ活性非常显著地下降（Ｐ＜０．０１）。结果表明：ＢＳＯ能有效地逆转Ａ５４９／Ｒ２细胞的抗药性或对其直接产生细胞毒作用；ＢＳＯ阻断细胞内ＧＳＨ合成，降低抗药细胞内ＧＳＨ水平或干扰ＧＳＴ活性，是对抗药细胞产生抗药性逆转或发挥直接杀伤效应的重要原因。     

HGPRT缺失和抗8-AG的人肺癌细胞(A_(549))突变株的建立

用ＭＮＮＧ处理Ａ５４９细胞和８－ＡＧ软琼脂集落形成方法，成功地筛选一株抗高浓度８－ＡＧ和完全缺失ＨＧＰＲＴ活性的人肺癌细胞突变株。该株细胞能抵抗８－ＡＧ的毒性作用，对ＨＡＴ选择培养基十分敏感。从而为体细胞融合提供亲本细胞。     

ACV治疗转TK基因人肺腺癌细胞A549裸鼠皮下成瘤的实验研究

目的 观察活体内转ＴＫ基因肺腺癌细胞对ＡＣＶ（无环鸟苷）的敏感性。方法 转ＴＫ基因肺腺癌细胞Ａ５４９－ＴＫ、亲代细胞Ａ５４９分别接种于裸鼠腹部皮下左右侧成瘤后,给予腹腔注射ＡＣＶ治疗２周,测量治疗前后肿瘤大小,并做病理观察。结果 Ａ５４９－ＴＫ细胞接种的肿瘤治疗前后体积增大不明显（Ｐ〉０．０５）;Ａ５４９细胞接种的肿瘤治疗后体积明显大于治疗前,光镜发现Ａ５４９－ＴＫ细胞接种的肿瘤有局部坏死,胞核裂     

干扰素α对人肺腺癌细胞系A549增殖抑制和诱导分化作用

目的：探讨干扰素α（ＩＦＮ－α）对人肺腺癌细胞的增殖抑制和诱导分化作用。方法：用不同质量浓度ＩＦＮ－α（２５μｇ／Ｌ,７５μｇ／Ｌ,１００μｇ／Ｌ）处理人肺腺癌Ａ５４９细胞,记录细胞数目,进行甲基绿－派洛宁染色,测定软琼脂集落形成率。结果：ＩＦＮ－α能明显抑制Ａ５４９细胞的增殖,药物组与对照组之间差异有显著性（Ｐ〈０．０５）,药物组与对照组比较增殖型细胞减少,分化型细胞数增加,双层软琼脂中细胞集落     

3种干扰因子对人肺癌细胞株A549体外增殖,侵袭力入ICAM— …

目的：研究全反维甲酸（ＡＴＲＡ）等干扰因子对肺癌细胞株Ａ５４９侵袭、无殖及ＩＣＡＭ－１分子表达的调节作用。方法：根据细胞穿过铺有Ｍａｔｒｉｇｅｌ胶多孔膜数量检测细胞侵袭力,用改良龙胆紫吸收比色计数法检测细胞增殖,以流式细胞分析和酶联免疫吸附试验检测ＩＣＡＭ－１、ｓＩＣＡＭ－１表达水平。结果：Ａ５４９经ＡＴＲＡ处理生,细胞表面ＩＣＡＭ－１表达降低,体外侵袭力明显降低,体外增殖明显抑制;ＩＦＮα不增加     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

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