高效液相色谱法同时测定四种抗癫痫药物的血药浓度

目的 建立用HPLC同时测定血清中多种抗癫痫药物卡马西平(carbamazepine,CBZ)、苯巴比妥(phenobarbital,PB)、硝西泮(nitrazepam,NTZ)、氯硝西泮(clonazepam,CNZ)等的方法. 方法 用一定方法处理血清样本,色谱柱为Nova-pak C18柱(150 mm ×3.9 mm,4μm);流动相为0.01 mol/L磷酸二氢钾缓冲液(pH 2.15)-乙腈(29∶71,V/V),监测波长223 nm;流速1 ml/min,柱温30℃. 结果 样品血清经处理,所留杂质不干扰被检测药品.在该色谱条件下以上药物能良好分离,在0.01-10 mg/L范围浓度与峰面积呈良好的线性关系(r＞0.999 0),方法回收率均大于90％,日内、日间RSD均小于10％.结论 该方法快速、准确、简便、实用,适用于以上治疗药物的治疗监测.     

高效液相色谱法同时测定血清中硝西泮、氯硝西泮的探讨

目的:建立一种同时测定血清中硝西泮与氯硝西泮的方法.方法:采用高效液相色谱法,色谱条件: ZORBAX 300SB-C8柱,柱温25℃,流动相:甲醇-水-乙腈=12∶68∶20,流速:1ml/min,检测波长:254nm;内标:150mg/L氢化可的松溶液.结果:两药在5～200μg/L的浓度范围内,药物-内标峰面积比(Y)与浓度(X)呈线性关系,最低检出浓度均为1μg/L;日内精密度分别为:硝西泮RSD=2.7%,氯硝西泮RSD=2.5%;硝西泮平均回收率为94.8%～104.0%,RSD为0.9%～3.2%;氯硝西泮平均回收率为96.3%～105.0%,RSD为1.2%～3.7%.结论:本方法快速、准确,适合对血清中两药的定性、定量分析.     

HPLC法同时测定人血清中氯硝西泮、硝西泮、三唑仑浓度

目的建立单步萃取-HPLC法同时测定人血清中硝西泮、氯硝西泮、三唑仑浓度。方法血清样品采用二氯甲烷单步萃取,采用Ultimate XB-C18色谱柱（250.0mm×4.6mm,5μm）,流动相为甲醇∶水∶乙腈=10∶56∶34,流速为1mL/min,检测波长为254nm,柱温为30℃,卡马西平为内标物。结果硝西泮、氯硝西泮、三唑仑的线性范围分别为32.5～650.0μg/L（r=0.9997）、31.5～630.0μg/L（r=0.9999）、74～1480μg/L（r=0.9997）,最低检测限分别为8.13μg/L、7.88μg/L、18.5mg/L,日内精密度和日间精密度良好,回收率较高。结论本法操作简便,结果准确可靠。可满足临床快速监测的需要。     

新型二维高效液相色谱测定人血清伏立康唑浓度方法的建立与应用

目的建立全自动二维高效色谱法(2D-HPLC)测定血清中伏立康唑的浓度并应用于临床。方法第一维色谱柱为ASTON SC2柱(4.6mm×25mm,5μm),流动相为20mmol/L磷酸铵∶乙腈∶甲醇=3∶1∶1(V∶V∶V,磷酸调pH=5.4),流速1.2mL/min;第二维色谱柱为SHIMADZU C18柱(4.6 mm×150 mm,5μm),流动相为10mmol/L醋酸铵(三乙胺调pH7.0)∶10mmol/L醋酸铵(三氟乙酸调pH3.0)∶甲醇∶乙腈=30∶10∶10∶50(V∶V∶V∶V),流速1.0mL/min;中间柱为ASTON SH柱(3mm×10mm,5μm),辅助流动相为纯水。样品由一维色谱柱萃取,经中间柱捕获并转移至第二维色谱柱分析,检测波长256nm,柱温40℃,进样量200μL。结果伏立康唑在0.28～22.56μg/mL范围内线性关系良好,r=0.999 9,最低定量限为0.28μg/mL,方法回收率为99.01%～104.62%,提取回收率为85.81%～89.82%,日内、日间精密度RSD均小于9%。结论该方法操作简单、准确、灵敏,符合血清样品的测定要求,适用于伏立康唑临床血药浓度测定。     

氯硝西泮单次给药与重复给药的实验研究

目的:研究氨硝西泮在大鼠血清及脑组织药物浓度的动态变化。方法:采用高效液相色谱法(HPLC)测定药物浓度,色谱柱为C18柱(200mm×4.6mm,4μm),流动相为甲醇∶水(55∶45),流速:0.8ml/min,检测波长310nm,柱温25℃。对48只健康成年SD大鼠血清及脑匀浆上清液经氯仿提取,挥干,用流动相重溶,进行色谱分析。结果:单次给药后大鼠血清及脑组织药物浓度快速下降,尤其是脑组织;重复给药后,血清及脑组织药物浓度再次升高。结论:氯硝西泮血清及脑组织中药物浓度的快速下降,可以导致惊厥复发。氯硝西泮重复给药可有效地控制惊厥复发,但也可引起药物在体内的蓄积。     

高效液相色谱-质谱法测定宣肺止嗽合剂中吗啡含量

目的建立高效液相色谱-质谱(HPLC-MS)法测定宣肺止嗽合剂中吗啡含量的方法。方法采用HPLC-MS正离子多反应监测模式(MRM)测定宣肺止嗽合剂中吗啡的含量,色谱柱为Shim-pack GIST-HP C18柱(50.0 mm×2.1 mm,3μm),流动相为甲醇-0.1%甲酸(30∶70,V/V),流速0.3 m L/min,柱温40℃;采用电喷雾离子源(ESI),以MRM进行定量分析,吗啡在正离子模式下定量分析离子对m/z 286. 40→m/z201.10。结果吗啡在0.517 5～5.175 0μg/m L质量浓度范围内线性关系良好(r=0.999 3);平均加样回收率为98.34%,RSD为1.44%;最低检测限为0.207 ng/m L,最低定量限为0.414 ng/m L。结论该方法简单可靠、重复性好,适用于药品中低含量吗啡的检测。     

反相高效液相色谱法测定神经酸片剂中神经酸含量

目的建立神经酸片剂中神经酸的反相高效液相色谱含量测定方法。方法色谱柱为UltimateR XB-C8 (4. 6 mm×250 mm,5μm),流动相为0.1%(V/V)磷酸水溶液-乙腈(93:7),检测波长为203 nm,流速1.0 m L/min,柱温25℃。结果神经酸与其他组分分离良好,神经酸浓度在0.01～0.2 mg/m L范围内线性关系良好(Y=3 000 000 X (mg/m L)+4 795.9,P=0.000,R2=0.999 9,n=5),该方法精密度实验RSD为1.80%(n=6)。样品溶液在24 h稳定,峰面积RSD为1.52%(n=6)。回收率为96.94%～104.46%(RSD在0.81%～3.80%之间)。结论首次采用C8柱,建立了一种反相高效液相色谱方法,实现了对片剂中神经酸含量的快速准确测定。     

固相萃取-反相HPLC荧光法快速测定血浆中氟罗沙星

目的建立固相萃取-反相HPLC荧光法快速测定人血浆中氟罗沙星的方法。方法采用C18固相萃取小柱萃取血浆中的氟罗沙星。色谱柱为ODS-BP柱(5μm,250 mm×4.6 mm),流动相为含1.31 g/L L-异亮氨酸和0.80 g/LCuSO4的水溶液-甲醇(80∶20,V∶V),激发波长为280 nm,发射波长为504 nm,流速为0.8 ml/min。结果该方法在0.4-20μg/ml浓度范围内线性关系良好(r=0.999 3),最小检测限50 ng/ml,萃取回收率和方法回收率分别为89.52%和100.45%,高、中、低3个浓度的日内RSD为3.2%,日间RSD为1.5%。结论该法血样处理简单,干扰小,灵敏度高,精密度好,可用于该药物体内监测的快速测定。     

RP-HPLC法测定人血浆中氯氮平浓度

目的:建立测定人血浆中氯氮平高效液相色谱法。方法:血浆样品经适当处理,色谱柱为Luna C18(250×4.6mm,5μm)柱,流动相为乙腈:30mmol/L醋酸铵(46:54,V/V),柱温40℃,流速0.9mL/min,检测波长243nm,艾司唑仑为内标。结果:测定方法在0.05-1.6μg/mL范围内线性良好(r=0.9992,n=6),高、中、低浓度加样回收率均在98.8%~104.0%之间,日内、日间RSD在2.96%~11.5%之间,最低检测浓度为0.01μg/mL(S/N=3)。结论:本方法快速、简便、准确,适用于临床氯氮平浓度监测。     

HPLC测定静脉给药后大鼠血清中得力生注射液中酯蟾毒配基与柔红霉素的含量

目的:建立大鼠血清中酯蟾毒配基与柔红霉素浓度的测定方法。方法:血清经乙酸乙酯提取,炔诺酮(酯蟾毒配基)、多柔比星(柔红霉素)作内标,采用高效液相色谱法测定酯蟾毒配基与柔红霉素浓度。色谱柱:phenomenex C18(250 mm×4.6 mm,5μm),流动相:甲醇-0.01 mol/L磷酸二氢铵溶液(pH 4.65)-冰醋酸(V∶V∶V=30∶20∶0.1)(柔红霉素),乙腈-水(55∶45)(酯蟾毒配基),检测波长:柔红霉素233 nm;酯蟾毒配基296 nm。结果:酯蟾毒配基在55.0～8800μg/L范围内,柔红霉素在0.1478～14.778 mg/L范围内,待测物与内标物的峰面积与浓度呈现良好线性关系,r=0.9997(酯蟾毒配基),r=0.9984(柔红霉素),平均回收率均>80%,日内、日间RSD均<5%(n=3)。结论:该方法快速、简便、准确,适合于临床得力生注射液与柔红霉素浓度的监测。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.