三种不同便潜血检测方法的对比观察

目的:比较三种不同便潜血检测方法的优劣性,寻找效果最好的联合检测方法。方法:使用化学法、单克隆抗体法和联合免疫法检测消化道肿瘤患者和正常人群便潜血,分别计算其阳性率。并使用不同浓度血红蛋白分别检测单克隆抗体法和转铁蛋白法的灵敏度。结果:化学法、单克隆抗体法和联合免疫法检测便潜血的阳性率分别是92.3%,94.6%和99.2%。转铁蛋白法灵敏度是0.1mg/L,单克隆抗体法灵敏度是0.02mg/L。结论:便潜血中转铁蛋白法的稳定性高于单克隆抗体法,但是灵敏度低于单克隆抗体法,所以两种方法的联合使用,可以很好的提高便潜血检测准确率。     

联合免疫法检测消化道粪便隐血结果分析

目的运用免疫法分析粪便隐血在消化道出血中的临床应用价值。方法分别采用血红蛋白免疫法和转铁蛋白免疫法对69例消化道出血病人及30例健康人的粪便进行隐血检测,其中上消化道出血23例,胃肿瘤病变14例,下消化道溃疡20例,大肠肿瘤病变12例。结果血红蛋白的免疫法检测阳性率分别为52．2％、71．4％、40．0％、66．7％;转铁蛋白免疫法检测阳性率分别为78．0％、85．7％、50．O％、83．3％;两种方法同时联合检测,其中任何一项阳性均判断为阳性,阳性率分别为87．0％、100％、65．0％、100％。结论粪便隐血检测仍是消化道出血疾病的筛检重要手段。血红蛋白与转铁蛋白的联合检测灵敏度高,特异性强,可有效地提高消化道出血及其恶性肿瘤病变的粪便隐血阳性检出率。     

单克隆抗体法检测尿中血红蛋白临床应用初探

目的 应用单克隆抗体法检测尿中血红蛋白，探讨鉴别尿中血红蛋白的准确而简便的方法，为临床提供可靠的实验依据。方法 测定单克隆抗体法及邻联苯胺法检测血红蛋白的灵敏度；应用单克隆抗体法及邻联苯胺法测定30例健康者及105例患者尿液。结果 单克隆抗体法检测尿血红蛋白最低检出浓度为0.21μg/ml，邻联苯胺法为1.66μg/ml。30例健康者尿液单克隆抗体法及邻联苯胺法检测结果均为阴性。105例患者尿液单克隆抗体法检测结果97例阳性，8例阴性；邻联苯胺法检测结果49例阳性，56例阴性，二者有显著性差异（P＜0.01)。结论 单克隆抗体法检测尿血红蛋白特异性强，灵敏度高，作为鉴别尿中有无血红蛋白的准确而简便的方法，可弥补干化学法和显微镜检测法的不足。     

转铁蛋白和血红蛋白联合检测消化道出血的临床应用

目的探讨血红蛋白法（OB）、转铁蛋白法（TF）和联合法检测消化道出血的临床应用价值。方法对1 944例临床标本分别使用转铁蛋白法、血红蛋白法和联合法进行潜血试验检测,并对结果进行比较分析。结果随机大便潜血试验阳性率转铁蛋白法为9.62%,血红蛋白法为5.76%,血红蛋白法与转铁蛋白法联合检测时为11.11%。其中,TF法、联合检测法与OB法阳性率比较,差异均有高度统计学意义（均P〈0.01）,TF法与联合检测法比较,差异无统计学意义（P〉0.05）。结论转铁蛋白法是检测大便潜血试验敏感的方法,血红蛋白法的敏感度最低。应用转铁蛋白法可以提高大便潜血的阳性检出率,有利于消化道出血性疾病的诊断和鉴别诊断。     

便潜血单克隆抗体法与联苯胺法特异性比较

为评价单克隆抗体法检测便潜血的特异性和准确性,对门诊及住院病人送检的便潜血100份随机标本用单克隆抗体法和联苯胺法检测粪便隐血。单克隆抗体法阳性率20％;联苯胺法阳性率36％,其中假阳性率16％。经临床证实20位确诊患者有消化道出血、直肠癌、胃癌、结肠炎、胃溃疡、子宫转移癌等疾病。16位假阳性的病人证实为食用过动物肉类或含过氧化物酶类蔬菜（如胡萝卜、西红柿、菠菜、韭菜、芹菜、油菜、木耳等）表明单克隆抗体法测定便潜血具有较高的准确性和特异性。     

粪便中血红蛋白转铁蛋白联合检测的临床价值研究

目的评价同时检测血红蛋白（Hb）与转铁蛋白（Tf）在粪便隐血试验中的临床价值。方法对180例消化道出血的患者及50例健康人的粪便标本分别用化学法（匹拉米洞半定量检测法）、单克隆抗体胶体金法进行隐血检测。化学法检测Hb、单克隆抗体胶体金法检测Hb和Tf。结果血红蛋白化学法（以下简称化学法）检测上消化道出血的阳性率为44.8%,检测下消化道出血的阳性率为57.5%,检测健康组阳性率为16.0%;血红蛋白单克隆抗体胶体金法（以下简称血红蛋白单克隆法）检测上消化道出血的阳性率为47.8%,检测下化道出血的阳性率66.3%,检测健康组阳性率为2.0%;转铁蛋白单克隆抗体胶体金法（以下简称转铁蛋白单克隆法）检测上消化道出血的阳性率为41.8%,检测下消化道出血的阳性率64.6%,检测健康组阳性率为2.0%;联合检测法（血红蛋白与转铁蛋白两次同时检测,有一项阳性即为阳性）检测上消化道出血的阳性率为53.7%,下消化道出血的阳性率为70.8%,健康组阳性率为2.0%。结论粪便中的Hb与Tf联合检测可显著提高消化道出血性疾病（包括胃、肠的溃疡、钩虫病、恶性肿瘤、息肉等）的阳性检出率。     

酶联法与联苯胺法检测粪潜血比较

目的：对便潜血关法与联苯胺法做对比分析。方法“对６０例临床送检便潜血的粪便标本同时用上述两法进行检测。结果：６０例便潜血粪便标本便潜血阳性检出率酶联法为２３．４％,联苯胺法为４０％;特异性试验中酶联法仅对人血蛋白阳性,而联苯胺法对人及动物的血红蛋白均为阳性。结论联苯胺法第三性虽高,但其特异性差,假阳性高;酶联法敏感快速快速,特异性强,是病人粪便潜血检查的首选试验。     

血红蛋白与转铁蛋白检测在消化道出血筛查中的应用

分别用血红蛋白免疫法与转铁蛋白免疫法对40例健康者及81例消化道出血患者的粪便进行检测,其中上消化道出血32例,胃癌6例,下消化道溃疡34例及直、结肿瘤9例。血红蛋白单克隆免疫法检测阳性率分别为46．9％、66．7％、32．3％、77．8％;转铁蛋白单克隆免疫法检测阳性率分别为71．9％、83．3％、47．1％、88．9％;两种方法联合检测,其中任何一项阳性即为阳性,阳性率分别为87．5％、100％、55．9％、100％。说明血红蛋白与转铁蛋白联合检测灵敏度高、特异性强。     

化学法、单克隆抗体法和联合免疫法检测便隐血的临床比较

目的：对比化学法、单克隆抗体法和联合免疫法检测便隐血的临床效果。方法选取大便标本90例,采用化学法、单克隆抗体法和联合免疫法3种试验方法,对其进行便隐血检测。结果化学法、单克隆抗体法和联合免疫法3种方式检测出血红蛋白的最低检出率分别为2、0.2、0.2 mg/L;化学法对人和动物的血红蛋白可发生反应,联合免疫法和单克隆抗体仅和人的血红蛋白发生反应;维生素C浓度超过10 g/L可引起化学法的阴性结果,而对联合免疫法和单克隆抗体的检测无影响。化学法、单克隆抗体法和联合免疫法3种不同的检测方法检测上消化道出血组的阳性率分别为95.2%、72.1%、91.2%,检测下消化道出血组的阳性率分别为75.6%、96.8%、100%;检测正常组的阳性率分别为10.5%、3.2%、6.8%。结论化学法、单克隆抗体法和联合免疫法3种检测方法相互结合、相互补充,有助于提高消化道出血性疾病的检出率,为临床提供更加精确的结果。     

胃液潜血单克隆抗体法假阴性实例试验初探

目的：针对胃液潜血单克隆抗体法假阴性实例进行初探试验。方法：采用联苯胺和单克隆抗体两种方法，对1例经不同方法处理的胃液标本同步进行潜血试验。结果：联苯胺法：经不同方法处理后的各组标本，测得结果均呈强阳性至阳性。单克隆抗体法：原胃液标本测得阴性，稀释后的A1～3组均测得阴性，加入血红蛋白液后的B1～3组测得结果均为阳性，孵育12h后的C1～3组结果为弱阳性至阳性，孵育24h后的D1～3组结果为阴性至弱阳性，单纯血红蛋白组结果为阳性。结论：该例胃液标本中存在内源或外源性物质，致使患血红蛋白免疫原活性降低或丧失，导致临检胃液潜血单克隆抗体法多次呈假阴性结果。提示，临床检验中应注意结合患临床症状，对可疑标本采用其他化学法复检，以求其真。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.