新生儿败血症血培养分离菌及耐药性分析

目的:了解我院近期新生儿败血症病原菌及耐药性,为临床用药提供依据。方法:对我院2001年1月至2006年1月新生儿血培养阳性标本细菌鉴定及药敏结果进行分析总结。结果:251例阳性标本中表皮葡萄球菌和金黄色葡萄球菌分别占31.5%和16.7%,对多种抗生素耐药,苯唑西林的耐药率分别为87.5%、62.6%,对万古霉素100%敏感;革兰阴性菌中大肠埃希菌占首位,其次为肺炎克雷伯杆菌、肠杆菌属、普通变形杆菌;抗菌活性较好的药物是阿米卡星、环丙沙星、氧氟沙星。结论:葡萄球菌是新生儿败血症的主要病原菌;临床医师应根据细菌鉴定及药敏试验选择敏感药物治疗。     

新生儿败血症病原菌及常见药敏分析

目的:了解新生儿败血症的病原菌及其常见药物敏感情况,指导抗菌药物的应用。方法:对新生儿败血症的血细菌培养阳性及药敏监测结果分析,药敏试验用M-H法,并按NCCCS标准判断细菌耐药性。结果:血细菌培养阳性61株,凝固酶阴性葡萄球菌(CNS)为主要病原菌,其中表皮葡萄球菌16株(26.2%),金黄色葡萄球菌占7株(11.4%),大肠埃希菌5株(8.1%),肺炎克雷伯菌4株(6.5%);细菌对青霉素、克林霉素、红霉素、头孢唑啉耐药性高,亚胺培南、头孢匹罗、头孢哌酮/舒巴坦、万古霉素最敏感。结论:重视新生儿败血症病原菌检测,根据血培养药敏结果选用敏感抗生素,可减少细菌耐药性的产生,提高临床治疗效果。     

106例新生儿败血症的细菌学分类及抗生素耐药性分析

目的：了解新生儿败血症病原菌及耐药性,为临床用药提供依据。方法：对106例新生儿血培养阳性标本进行细菌鉴定及药敏结果进行分析。结果：106例阳性标本中金黄色葡萄球菌和表皮葡萄球菌分别占42.5%和14.2%,对多种抗生素耐药,苯唑西林的耐药率分别为63.6%、88.6%,对万古霉素100%敏感;革兰阴性菌中大肠埃希菌占首位,其次为洛菲不动杆菌,抗菌活性较好的药物是阿米卡星、环丙沙星。结论：葡萄球菌是新生儿败血症的主要病原菌;临床医师应根据细菌鉴定及药敏试验选择敏感药物治疗。     

新生儿败血症病原菌分类及耐药性分析

目的分析探讨新生儿败血症的病原菌分类及耐药性。方法选取我院新生儿败血症130例,均为2007年10月～2010年10月儿科患儿的血培养,对其临床资料进行回顾分析。结果 130例败血症患儿的血培养的病原菌有革兰氏阳性菌和革兰氏阴性菌,其中以葡萄球菌为主,对大多数抗生素耐药。结论根据细菌鉴定和药敏试验来选择敏感的药物进行治疗,掌握病原菌分类及耐药性的变化,对提高新生儿抗感染治疗具有重要意义。     

87例新生儿败血症的病原菌及耐药性分析

目的研究宜兴市人民医院新生儿败血症病原菌及药物敏感情况,指导临床合理应用抗菌药物。方法对宜兴市人民医院2006年1月至2010年lO月新生儿败血症血培养阳性标本共87例进行细菌鉴定及药敏结果分析。结果87例血培养阳性标本中,革兰阳性球菌61株,占70．1％,其中以凝固酶阴性葡萄球菌（CNS）为主,占56．3％,其次为金黄色葡萄球菌,占9．2％,CNS对多种抗生素耐药,对万古霉素及替考拉宁100％敏感;革兰阴性杆菌26株,占29．9％,其中肺炎克雷伯菌12株,占13．8％,其次为大肠埃希菌10株,占11．5％,二者产超广谱B内酰胺酶高,对其抗菌活性较好的是加酶抑制剂的B内酰胺类抗生素,如头孢哌酮／舒巴坦,对亚胺培南高度敏感。结论宜兴市人民医院新生儿败血症病原菌以CNS为主,其次为肺炎克雷伯菌,大肠埃希菌和金黄色葡萄球菌;并且上述病原菌具多重耐药性,应加强病原菌种类及耐药性监测,合理应用抗生素。     

新生儿败血症病原菌分布及耐药性分析

目的探讨新生儿败血症的病原菌种类及耐药性,指导临床合理使用抗生素。方法对366份新生儿血培养阳性标本的细菌进行鉴定及药敏分析。结果366株病原菌中G^＋菌为80．9％（以表皮葡萄球菌占首位,占41．2％,其次为金黄色葡萄球菌,占29．8％。）,对常用的一线抗生素如青霉素、苯唑西林、庆大霉素耐药率高,对万古霉素无耐药。G^-菌为15．8％（以大肠埃希菌、肺炎克雷伯菌及肠杆菌属为主,共占81．0％）,对第一、二代头孢等敏感率很低,大多数几乎完全耐药,对三代头孢耐药率较低,亚胺培南无耐药。真菌为3．3％。结论引起新生儿败血症的细菌以革兰氏阳性球菌为主,病原菌耐药呈增高趋势,全面了解和掌握细菌种类和耐药性变化,提高新生儿抗感染治疗的疗效,降低耐药性的发生。     

新生儿败血症血培养中病原菌分布及耐药性分析

目的分析我院住院新生儿败血症患儿血培养病原菌的分布及药敏情况。方法采用BACTEC9050全自动血培养仪培养,MicroScan auto vSCAN-4微生物鉴定/药敏分析仪对分离出的细菌进行鉴定及药敏分析。结果1016例标本中分离鉴定出154株细菌,阳性率15.2%,革兰阳性菌占80.5%,以凝固酶阴性葡萄球菌（CNS）占多数（60.4%）,其次是肠球菌（9.1%）,金黄色葡萄球菌（5.2%）;革兰阴性菌以大肠埃希菌占多数（10.5%）。药敏结果除葡萄球菌、肠球菌对万古霉素100%敏感,大肠埃希菌、肺炎克雷伯菌对亚胺培南100%敏感外,其余抗菌药物均有不同程度的耐药。结论CNS为我院新生儿败血症的主要病原菌,血培养检出病原菌及其耐药性是临床诊治的重要参考。     

新生儿血培养常见病原菌的分布及药物敏感率分析

目的探讨新生儿败血症的病原菌分布及药物敏感情况，以合理地选择抗生素。方法无菌抽取临床怀疑为败血症的新生儿血液进行培养、鉴定及药敏实验。结果820份血培养分离出病原菌174株，其中G^＋球菌135株，以凝固酶阴性葡萄球菌为主，其次为金黄色葡萄球菌；G^-杆菌38株。G^＋球菌对青霉素、苯唑青霉素、红霉素等敏感率较低，G^-菌对哌拉西林、氨苄西林及第二、三代头孢等有较高的耐药性。结论葡萄球菌、克雷伯菌属、大肠埃希菌及肠杆菌属是新生儿败血症的主要病原菌；所有细菌对常用抗生素有较高的耐药性。     

新生儿败血症76例血培养阳性结果及耐药性分析

目的:了解新生儿败血症病原学特点并分析致病菌耐药情况,为临床及时明确病原、正确选用抗生素提供依据.方法:对新生儿败血症患儿76例血培养检出细菌及药敏结果进行回顾性分析.结果:临床诊断败血症的血液标本中检出病原菌76株,其中革兰阳性菌71株(93.4%),前3位的是表皮葡萄球菌35株(46.0%),溶血性葡萄球菌20株(26.3%),肠球菌6株(7.9%);革兰阴性菌4株(5.3%);假丝酵母菌1株(1.3%).革兰阳性菌对青霉素、阿莫西林克拉维酸钾、氨苄西林、苯唑西林耐药性高,对万古霉素、利奈唑胺无耐药性.结论:重视新生儿败血症病原菌耐药性检测,根据血培养药敏结果选择敏感的抗菌药物,合理使用抗生素,可减少细菌耐药性的产生,提高临床治疗效果.     

新生儿败血症274例病原菌分布及耐药性调查研究

目的:调查274例新生儿败血症病原菌分布及耐药情况,以指导临床合理用药。方法:回顾性分析274例败血症新生儿的临床资料,采取病例对照研究法,根据不同发病时间,分为早发型败血症98例、晚发型败血症176例,研究病原菌分布及耐药性。结果:败血症患儿的血标本病原菌培养为单一致病菌,主要为革兰阳性球菌、革兰阴性杆菌、真菌,分别是154株(56.2%),114株(41.6%),6株(2.2%);早发型败血症病原菌主要是革兰阴性菌(57.1%);晚发型败血症病原菌主要是革兰阳性菌(64.2%);早发型新生儿败血症凝固酶阴性葡萄球菌、表皮葡萄球菌感染比例明显低于晚发型新生儿败血症(P<0.05),晚发型新生儿败血症大肠埃希菌感染比例明显低于早发型新生儿败血症(P<0.05)。革兰阳性菌中,金黄色葡萄球菌、凝固酶阴性葡萄球菌、表皮葡萄球菌,对万古霉素的敏感性最高,对青霉素G敏感率最低;革兰阴性菌中,大肠埃希菌、肺炎克雷伯菌、阴沟肠杆菌对美罗培南、亚胺培南的敏感性最高。结论:新生儿早发型、晚发型败血症常见病原菌不同,临床在治疗时应根据细菌鉴定、药敏试验结果合理选择抗生素药物,并注意监测耐药性。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.